Demet Erdag, Serkan N Koc, M Faruk Oksuzomer, Leman Yalcintepe
{"title":"Synergistic effect of selenium and genipin triggers viability of 3T3 cells on PVA/Gelatin scaffolds.","authors":"Demet Erdag, Serkan N Koc, M Faruk Oksuzomer, Leman Yalcintepe","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>The aim of this study was to reveal the first time synergistic effect of GP and selenium (Se) on 3T3 cells seeded on natural and non-cytotoxic porous scaffolds with poly(vinyl alcohol) (PVA) and gelatin (GE).</p><p><strong>Methods: </strong>Electrospinning scaffolds were produced as PVA/GE/GA crosslinked with glutaraldehyde (GA) and freeze/dried scaffolds crosslinked with genipin (GP) were divided into two groups as PVA/GE/GP5 and PVA/GE/GP8. The scaffolds were investigated in terms of pore morphology, swell ratio, biodegradation, and biocompatibility. The biocompatibility of the material was tested in vitro by MTT assay on 1, 2, and 3 days to test the cell viability of 3T3 cells.</p><p><strong>Results: </strong>It was observed that Se triggered the excellent cell growth and proliferation on electrospinning and freeze drying PVA/GE scaffolds.</p><p><strong>Conclusions: </strong>Selenium with PVA/GE scaffolds can be a promising candidate for wound healing application, as it significantly increases cell viability on scaffolds. It is thought that the synergistic effect of selenium with genipin may be an important step in tissue engineering applications. The preliminary study can be supported by in vivo studies in the future.</p>","PeriodicalId":6897,"journal":{"name":"Acta of bioengineering and biomechanics","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139680505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Justyna Kędziorek, Michalina Błażkiewicz, Katarzyna Kaczmarczyk
{"title":"Using nonlinear measures to evaluate postural control in healthy adults during bipedal standing on an unstable surface.","authors":"Justyna Kędziorek, Michalina Błażkiewicz, Katarzyna Kaczmarczyk","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>This study examined the use of nonlinear measures - sample entropy (SampEn), fractal dimension (FD), and the Lyapunov exponent (LyE) - to evaluate postural control in adults during standing on an unstable surface, with and without visual feedback.</p><p><strong>Methods: </strong>14 healthy young adults (24.07 ± 7.32 years) completed bipedal standing trials on an unstable-plate Biodex Balance System (BBS) connected to a Vicon system, with eyes open and closed. Each trial lasted 20 sec. Analysis was performed based on the center of mass (CoM), for which the three nonlinear measures were calculated.</p><p><strong>Results: </strong>Excluding visual feedback was found to cause a significant increase in linear and nonlinear parameters. Moreover, SampEn and FD values were found to be significantly higher in the PD direction, compared to AP or ML, whereas LyE values in this direction were minimal.</p><p><strong>Conclusions: </strong>Results show that the three nonlinear measures provide a useful way of evaluating postural control in healthy adults. Moreover, it seems that introducing an unstable surface meant that the projection of the CoM was not perpendicular to the surface, but rather set at a certain continually changing angle, forcing the whole system to adapt to chaotic and unpredictable conditions. Such refined changes in conditions can be evaluated in a precise way only by using nonlinear measures.</p>","PeriodicalId":6897,"journal":{"name":"Acta of bioengineering and biomechanics","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139680507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Julia Lisoń, Anna Taratuta, Zbigniew Paszenda, Marcin Dyner, Marcin Basiaga
{"title":"A study on the physicochemical properties of surface modified Ti13Nb13Zr alloy for skeletal implants.","authors":"Julia Lisoń, Anna Taratuta, Zbigniew Paszenda, Marcin Dyner, Marcin Basiaga","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>As it is widely stated in the literature, biofilms are responsible for most chronic infections, which have grown exponentially over the past three decades. The use of so-called alloys, as a new generation of materials, enables us to find the golden mean in the arena widely known as implantology. The use of the surface layer, using the chosen Atomic Layer Deposition method, is to be the basis for minimizing the risk of an organism reactions. Therefore, the primary objective of this study was to observe the impact of physicochemical properties of the surface layers (bactericidal) on the processes that occur on the implants surface made of titanium biomaterials used in bone structures. The study also attempted to evaluate the physicochemical properties of the ZnO coatings, deposited on the substrate of one of the new generation Ti13Nb13Zr alloys, using the ALD method. Included in the assessment of the physicochemical properties of the surface layers formed in this manner, we perform pitting corrosion resistance tests, scratch tests, tribological tests and surface wettability tests. Based on the obtained data, the differing physicochemical properties of the alloy with ZnO coatings are found to be dependent on the applied surface modification. For the conducted tests, differences are determined for the tests on the corrosion resistance, surface wettability and the abrasion resistance for samples with and without the ZnO coating. In addition, tests show that the coating applied to the alloy, which is previously subjected to the sand-blasted process, is characterized by improved adhesion.</p>","PeriodicalId":6897,"journal":{"name":"Acta of bioengineering and biomechanics","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139680528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Iwona Zarzyka, Anna Czerniecka-Kubicka, Karol Hęclik, Lucjan Dobrowolski, Beata Krzykowska, Anita Białkowska, Mohamed Bakar
{"title":"Biobased poly(3-hydroxybutyrate acid) composites with addition of aliphatic polyurethane based on polypropylene glycols.","authors":"Iwona Zarzyka, Anna Czerniecka-Kubicka, Karol Hęclik, Lucjan Dobrowolski, Beata Krzykowska, Anita Białkowska, Mohamed Bakar","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Poly(3-hydroxybutyrate) (P3HB) is the most important of the polyhydroxyalkanoates. It is biosynthesized, biodegradable, biocompatible, and shows no cytotoxicity and mutagenicity. P3HB is a natural metabolite in the human body and, therefore, it could replace the synthetic, hard-to-degrade polymers used in the production of implants. However, P3HB is a brittle material with limited thermal stability. Therefore, in order to improve its mechanical properties and processing parameters by separating its melting point and degradation temperature, P3HB-based composites can be produced using, for example, linear aliphatic polyurethanes as modifiers. The aim of the study is a modification of P3HB properties with the use of linear aliphatic polyurethanes synthesized in reaction of hexamethylene diisocyanate (HDI) and polypropylene glycols (PPG) by producing their composites. Prepared biocomposites were tested by the scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and thermogravimetry (TGA). Furthermore, selected mechanical properties were evaluated. It has been confirmed that new biocomposites showed an increase in impact strength, relative strain at break, decrease of hardness and higher degradation temperature compared to the unfilled P3HB. The biocomposites also showed a decrease in the glass transition temperature and the degree of crystallinity. Biocomposites obtained with 10 wt. % polyurethane synthesized with polypropylene glycol having 1000 g ⋅ mole-1 and HDI have the best thermal and mechanical properties.</p>","PeriodicalId":6897,"journal":{"name":"Acta of bioengineering and biomechanics","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139680531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amadeusz Bartoszek, Artur Struzik, Sebastian Jaroszczuk, Marek Woźniewski, Bogdan Pietraszewski
{"title":"Comparison of the optoelectronic BTS Smart system and IMU-based MyoMotion system for the assessment of gait variables.","authors":"Amadeusz Bartoszek, Artur Struzik, Sebastian Jaroszczuk, Marek Woźniewski, Bogdan Pietraszewski","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>Although inertial measurement unit (IMU)-based systems have been validated against optoelectronic systems for recording joint kinematics, the accuracy of each system must be evaluated, and measurements from different systems cannot be easily compared. Therefore, this study compared the joint angles recorded using the IMU-based MyoMotion system and the optoelectronic BTS Smart-DX 700 system during Nordic walking.</p><p><strong>Methods: </strong>The study subject, a long-time Nordic walking instructor, was assigned to walk 12 m/trial (14 trials with 5 sampled gait cycles) at a velocity preferred for Nordic walking. The trials were simultaneously recorded by both systems. The instantaneous lower (ankle, knee, hip) and upper (shoulder, elbow, wrist) limb joint angles were recorded.</p><p><strong>Results: </strong>The joint angles from MyoMotion were significantly larger or smaller (depending on the joint and plane) than those from BTS.</p><p><strong>Conclusions: </strong>Joint angles measured by MyoMotion are not interchangeable with values from BTS, and IMU-recorded values should be interpreted carefully. However, MyoMotion can still provide information about intra-individual changes based on the joint angle profiles, e.g., following Nordic walking training.</p>","PeriodicalId":6897,"journal":{"name":"Acta of bioengineering and biomechanics","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139680532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Noor Arifah Azwani Abdul Yamin, Khairul Salleh Basaruddin, Shahriman Abu Bakar, Ahmad Faizal Salleh, Mohd Hanafi Mat Som, Asyraf Hakimi Abu Bakar
{"title":"Lower extremity joint reaction forces and plantar fascia strain responses due to incline and decline walking.","authors":"Noor Arifah Azwani Abdul Yamin, Khairul Salleh Basaruddin, Shahriman Abu Bakar, Ahmad Faizal Salleh, Mohd Hanafi Mat Som, Asyraf Hakimi Abu Bakar","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>The present study aims to investigate the effect of incline and decline walking on ground and joint reaction forces (JRF) of lower extremity and plantar fascia strain (PFS) under certain surface inclination angles.</p><p><strong>Methods: </strong>Twenty-three male subjects walked on a customized platform with four different surface inclinations (i.e., 0, 5, 7.5 and 10°) with inclined and declined directions. The motion of the ten reflective markers was captured using Qualysis motion capture system (Qualysis, Gothenburg, Sweden) and exported to a visual three-dimensional (3D) software (C-motion, Germantown, USA) in order to analyze the GRF, JRF and PFS.</p><p><strong>Results: </strong>The results found that the peak vertical GRF is almost consistent for 0 and 5° inclination slope but started to decrease at 7.5° onwards during decline walking. The most affected JRF was found on knee at medial-lateral direction even as low as 5 to 10° inclination for both walking conditions. Furthermore, the findings also show that the JRF of lower extremity was more affected during declined walking compared to inclined walking based on the number of significant differences observed in each inclination angle. The PFS was found increased with the increase of surface inclination.</p><p><strong>Conclusions: </strong>The findings could provide a new insight on the relationship of joint reaction forces and strain parameter in response to the incline and decline walking. It would benefit in providing a better precaution that should be considered during hiking activity, especially in medial-lateral direction in order to prevent injury or fall risk.</p>","PeriodicalId":6897,"journal":{"name":"Acta of bioengineering and biomechanics","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139680537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characteristic research of lower extremity injuries in elderly pedestrians during collisions.","authors":"Sen Xiao, Lidong Zhang, Jindong Wu, Xinran Liu, Xiaoang Liu, Hao Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>The aim of this research is to study the trend of pedestrian lower extremity injuries during vehicle-pedestrian collisions.</p><p><strong>Methods: </strong>In this study, pedestrian's age, collision angle and pedestrian's position are considered influencing factors. Nine experiments using a novel lower extremity mechanical model are designed with the orthogonal experiment method.</p><p><strong>Results: </strong>Under the same collision angle, collisions in the left and right positions caused more serious tibia injuries than the middle position. As for the collision angle, the tibial injury at +45° is more significant than the tibial injury at -45°, and the injury of oblique collisions is slightly greater than that at 0°. Moreover, tibial injury is more sensitive to research variables than femoral injury. When the collision angle and position are changed, the difference ratio of tibia stress is by 483.2% higher than that of femur stress. The axial force and bending moment of the quadriceps tendon in the left-position collision reach peak values, which are 3.83 kN and 165.98 Nm, respectively. The peak quadriceps tendon axial force is captured with the collision angle of -45°, and the peak quadriceps tendon bending moment is obtained with a collision angle of +45°.</p><p><strong>Conclusions: </strong>The effects of differences in impact position and angle on lower extremity injury in the elderly were analyzed, and the results of this study can be used as a reference for research on lower extremity protection.</p>","PeriodicalId":6897,"journal":{"name":"Acta of bioengineering and biomechanics","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9676704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Demet Erdag, S. Koç, M. F. Öksüzömer, L. Yalcintepe
{"title":"Synergistic effect of selenium and genipin triggers viability of 3T3 cells on PVA/Gelatin scaffolds","authors":"Demet Erdag, S. Koç, M. F. Öksüzömer, L. Yalcintepe","doi":"10.35248/2168-9296.21.S1.236","DOIUrl":"https://doi.org/10.35248/2168-9296.21.S1.236","url":null,"abstract":"Purpose: The aim of this study was to reveal the first time synergistic effect of GP and selenium (Se) on 3T3 cells seeded on natural and non-cytotoxic porous scaffolds with poly(vinyl alcohol) (PVA) and gelatin (GE). Methods: Electrospinning scaffolds were produced as PVA/GE/GA crosslinked with glutaraldehyde (GA) and freeze/dried scaffolds crosslinked with genipin (GP) were divided into two groups as PVA/GE/GP5 and PVA/GE/GP8. The scaffolds were investigated in terms of pore morphology, swell ratio, biodegradation, and biocompatibility. The biocompatibility of the material was tested in vitro by MTT assay on 1, 2, and 3 days to test the cell viability of 3T3 cells. Results: It was observed that Se triggered the excellent cell growth and proliferation on electrospinning and freeze drying PVA/GE scaffolds. Conclusions: Selenium with PVA/GE scaffolds can be a promising candidate for wound healing application, as it significantly increases cell viability on scaffolds. It is thought that the synergistic effect of selenium with genipin may be an important step in tissue engineering applications. The preliminary study can be supported by in vivo studies in the future.","PeriodicalId":6897,"journal":{"name":"Acta of bioengineering and biomechanics","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69995233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aitor Marcos-Blanco, F. García-Pinillos, Alejandro Molina-Molina, D. Jaén-Carrillo, V. Soto-Hermoso, Santiago A. Ruiz-Alias
{"title":"Reliability of the RunScribe™ system to determine kinematic variables of the pelvis during locomotion at different speeds","authors":"Aitor Marcos-Blanco, F. García-Pinillos, Alejandro Molina-Molina, D. Jaén-Carrillo, V. Soto-Hermoso, Santiago A. Ruiz-Alias","doi":"10.37190/abb-02073-2022-03","DOIUrl":"https://doi.org/10.37190/abb-02073-2022-03","url":null,"abstract":"Purpose This study aimed to determine the reliability of the RunScribe™ system to measure kinematic variables of the pelvis during walking and running. Methods In this study, a treadmill protocol was implemented where the participants (n=23) completed 3 sets of 1 minute at 5, 10 and 15 km.h-1. Results All the recorded measurements during walking reported a low reliability with coefficients of variation (CV) greater than 10% in all variables and small-moderate intraclass correlation coefficient (ICC) (< 0.6) in seven out of ten variables. Similarly, the CVs reported in running were greater than 10% except for the maximum angular rate in the obliquity of the pelvis and the vertical oscillation that together with the angular velocity variables showed almost perfect ICCs (> 0.92). Conclusions Therefore, the data obtained suggests that the RunScribe™ system with 3 IMUs does not provide reliable metrics about the kinematics of the pelvis during locomotion (i.e., walking and running).","PeriodicalId":6897,"journal":{"name":"Acta of bioengineering and biomechanics","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69999362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Han Jiang, A. Kruszewski, E. Cherkashina, A. Theodorou, E. Zacharakis, I. Cherkashin, Igor Konovalov, M. Kruszewski
{"title":"Compensatory action of the index and middle finger in the kinematic chain of a basketball shot","authors":"Han Jiang, A. Kruszewski, E. Cherkashina, A. Theodorou, E. Zacharakis, I. Cherkashin, Igor Konovalov, M. Kruszewski","doi":"10.37190/abb-02092-2022-03","DOIUrl":"https://doi.org/10.37190/abb-02092-2022-03","url":null,"abstract":"Purpose Performing effective actions requires the basketball player to balance factors such as motor variability, error minimalization and a complex sequence of coordination to determine the best action. Aim of study. The aim of the study was to differentiate the strength of the muscles of the index and middle fingers when performing a basketball shot. Methods Material and method. Study group enrolled 122 male college basketball league students. The study included psychophysiological tests to determine indices of individual and typological characteristics of higher nervous activity, proprioceptive sensitivity tests of the fingers of the leading hand, and field tests to assess participants' shooting skills. The touch-based finger pressure sensing system measured the different levels of pressure exerted by the participants' main index and middle finger during grasping. Results For both the middle and index finger, the highest correlation with shot efficiency was found for a 120 g load g (p < 0,01 for 2PS; 2PS40 suc; FT; 3PSO and 3,5mS). Furthermore, high reproducibility of proprioceptive sensitivity of the index and middle finger of the leading hand was found in basketball players. Conclusions The research indicates that it is possible to organize compensatory behavior between joints on the basis of proprioception, with the last compensatory movements of the kinematic chain being performed by the fingers of the hand. The demonstrated high proprioceptive sensitivity of the index and middle finger of the leading hand in basketball players at a weekly interval may indicate ability to maintain high repeatability of movements controlled by these fingers.","PeriodicalId":6897,"journal":{"name":"Acta of bioengineering and biomechanics","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69999405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}