Synergistic effect of selenium and genipin triggers viability of 3T3 cells on PVA/Gelatin scaffolds.

IF 0.8 4区医学 Q4 BIOPHYSICS

Acta of bioengineering and biomechanics Pub Date : 2022-01-01

Demet Erdag, Serkan N Koc, M Faruk Oksuzomer, Leman Yalcintepe

{"title":"Synergistic effect of selenium and genipin triggers viability of 3T3 cells on PVA/Gelatin scaffolds.","authors":"Demet Erdag, Serkan N Koc, M Faruk Oksuzomer, Leman Yalcintepe","doi":"","DOIUrl":null,"url":null,"abstract":"Purpose: The aim of this study was to reveal the first time synergistic effect of GP and selenium (Se) on 3T3 cells seeded on natural and non-cytotoxic porous scaffolds with poly(vinyl alcohol) (PVA) and gelatin (GE).Methods: Electrospinning scaffolds were produced as PVA/GE/GA crosslinked with glutaraldehyde (GA) and freeze/dried scaffolds crosslinked with genipin (GP) were divided into two groups as PVA/GE/GP5 and PVA/GE/GP8. The scaffolds were investigated in terms of pore morphology, swell ratio, biodegradation, and biocompatibility. The biocompatibility of the material was tested in vitro by MTT assay on 1, 2, and 3 days to test the cell viability of 3T3 cells.Results: It was observed that Se triggered the excellent cell growth and proliferation on electrospinning and freeze drying PVA/GE scaffolds.Conclusions: Selenium with PVA/GE scaffolds can be a promising candidate for wound healing application, as it significantly increases cell viability on scaffolds. It is thought that the synergistic effect of selenium with genipin may be an important step in tissue engineering applications. The preliminary study can be supported by in vivo studies in the future.","PeriodicalId":6897,"journal":{"name":"Acta of bioengineering and biomechanics","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta of bioengineering and biomechanics","FirstCategoryId":"5","ListUrlMain":"","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOPHYSICS","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose: The aim of this study was to reveal the first time synergistic effect of GP and selenium (Se) on 3T3 cells seeded on natural and non-cytotoxic porous scaffolds with poly(vinyl alcohol) (PVA) and gelatin (GE).

Methods: Electrospinning scaffolds were produced as PVA/GE/GA crosslinked with glutaraldehyde (GA) and freeze/dried scaffolds crosslinked with genipin (GP) were divided into two groups as PVA/GE/GP5 and PVA/GE/GP8. The scaffolds were investigated in terms of pore morphology, swell ratio, biodegradation, and biocompatibility. The biocompatibility of the material was tested in vitro by MTT assay on 1, 2, and 3 days to test the cell viability of 3T3 cells.

Results: It was observed that Se triggered the excellent cell growth and proliferation on electrospinning and freeze drying PVA/GE scaffolds.

Conclusions: Selenium with PVA/GE scaffolds can be a promising candidate for wound healing application, as it significantly increases cell viability on scaffolds. It is thought that the synergistic effect of selenium with genipin may be an important step in tissue engineering applications. The preliminary study can be supported by in vivo studies in the future.

本刊更多论文

硒和吉尼平的协同效应可激发 PVA/明胶支架上 3T3 细胞的活力。

目的：本研究旨在首次揭示GP和硒（Se）对播种在天然无细胞毒性的多孔聚乙烯醇（PVA）和明胶（GE）支架上的3T3细胞的协同作用：用戊二醛（GA）交联的电纺支架为 PVA/GE/GA，用基因素（GP）交联的冻干支架分为 PVA/GE/GP5 和 PVA/GE/GP8 两组。从孔隙形态、膨胀率、生物降解和生物相容性等方面对支架进行了研究。通过 MTT 试验在体外测试材料的生物相容性，测试 3T3 细胞在 1 天、2 天和 3 天的存活率：结果：在电纺丝和冷冻干燥 PVA/GE 支架上观察到，硒引发了细胞的良好生长和增殖：结论：硒与 PVA/GE 支架可显著提高支架上的细胞存活率，是伤口愈合应用的理想候选材料。硒与吉尼平的协同作用可能是组织工程应用的重要一步。这项初步研究可在未来的体内研究中得到支持。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Acta of bioengineering and biomechanics BIOPHYSICS-ENGINEERING, BIOMEDICAL

CiteScore

2.10

自引率

10.00%

发文量

期刊介绍： Acta of Bioengineering and Biomechanics is a platform allowing presentation of investigations results, exchange of ideas and experiences among researchers with technical and medical background. Papers published in Acta of Bioengineering and Biomechanics may cover a wide range of topics in biomechanics, including, but not limited to: Tissue Biomechanics, Orthopedic Biomechanics, Biomaterials, Sport Biomechanics.