海军军医大学学报最新文献

{"title":"HPLC in simultaneous determination of seven main flavonoids in Epimediumof different places of origin","authors":"Mi-na Huang, Yan-ni Zhou, Qiang Liu, Jin Zhou, L. Lv","doi":"10.3724/SP.J.1008.2015.01352","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.01352","url":null,"abstract":"Objective To determine the contents of seven main flavonoids in Epimediumof different places of origin by high performance liquid chromatography(HPLC).Methods The HPLC condition was as follows:SHISEIDO MG-C18column(3.0mm×100 mm,3.0μm);the mobile phase consisted of acetonitrile and water containing 0.1% formic acid was used as gradient elute;the gradient of acetonitrile was 25%(0-10min),25%-40%(10-12min),40%-45%(12-22min),45%-75%(22-25min)and 75%(25-30 min);flow rate was 0.6 mL/min;UV detection wavelength was set at 270 nm;column temperature was 25℃;and injection volume was 5μL.The sample was ultrasonically extracted with 70% ethanol solution.Results The seven flavonoids,including epimedin A,epimedin B,epimedin C,icariin,icariside Ⅰ,icariside Ⅱ and anhydroicaritin were separated at baseline within 30 min.The standard curves had good linearity(r=0.999 9),with the RSD%of intraday and interday precision being both less than 2.0%,the recovery being 98%-102%,and the RSD% of stability and repeatability also being less than 2.0%.The results showed that the method could meet the requirements of method validation.The contents of seven flavonoids in reference crude meterial and Herba Epimedii from Liaoning,Gansu and Hubei province were determined.Conclusion The present method is rapid and simple,and can be used for quality control of Epimedium,which may lay a foundation for studying the pharmacokinetics and tissue distribution of the flavonoids of Epimedium.","PeriodicalId":6893,"journal":{"name":"Academic Journal of Second Military Medical University","volume":"36 1","pages":"1352"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69859890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Presetting negative pressure drainage for anastomotic leakage after esophageal cancer surgery: a clinical comparative analysis","authors":"Kenan Huang, Zhi-fei Xu, Xinyu Ding, Xu Li, Chen Qi, Bin Wu, Hua Tang","doi":"10.3724/SP.J.1008.2015.01356","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.01356","url":null,"abstract":"","PeriodicalId":6893,"journal":{"name":"Academic Journal of Second Military Medical University","volume":"36 1","pages":"1356"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69859898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modified microneedle roller enhancing percutaneous absorption of triamcinolone acetonide by human hyperplastic scar skin","authors":"Ya Li, Juan-juan Zhang, Li-Chao Zhang, Quan‐gang Zhu, Hai-jin Yu, Zhijuan Wu","doi":"10.3724/SP.J.1008.2015.00058","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.00058","url":null,"abstract":"Objective To evaluate the role of modified microneedle roller in promoting permeation of triamcinolone acetonide across human hyperplastic scar skin.Methods The morphology of human hyperplastic scar skin was visualized by methylene blue staining and H-E staining after the insertion by microneedles of different lengths.The content of triamcinolone acetonide was determined by high performance liquid chromatography(HPLC).The in vivo absorption characteristics across human hyperplastic scar skin were investigated by elution method and the homogenization method at 1hand 12 hafter drug application.Skin irritation was assessed by transepidermal water-loss(TEWL)measurement and laser Doppler flowmetry.Results The preferred microneedle length of 500μm effectively penetrated through the stratum corneum,and the distribution of the microchannels was homogeneous.It was found that 27.42% and 60.64% of the drug administered entered the skin at 1h and 12 h,respectively.The drug accumulation in the skin at 12 hdecreased to 45.98% of that at 1hafter intracutaneous injection,while the accumulation increased to 2.73-fold and 4.18-fold in the microneedle group and direct application of triamcinolone acetonide cream group,respectively,with the content of microneedle group being 3.56-fold that of the direct cream application group(P0.05).Triamcinolone acetonide distribution was not homogeneous at 12 hafter intracutaneous injection,with the level being(4.83±5.51)μg,while the distribution in the microneedle group was homogenous,with the level being(0.93±0.14)μg.TEWL results showed no significant difference in skin irritation between microneedle and intracutaneous injection group;however,laser Doppler flowmetry showed that the skin irritation of intracutaneous injection group was 8.40-fold that of microneedle treatment group.Conclusion Modified microneedle roller treatment followed by cream application,compared with intracutaneous injection,can yield more homogeneous distribution and less skin irritation in human hyperplastic scar skin,but its efficacy for scar treatment remains to be further verified.","PeriodicalId":6893,"journal":{"name":"Academic Journal of Second Military Medical University","volume":"36 1","pages":"58"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69842542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physical therapy in grassroot military medical units of PLA: current situation","authors":"Fanfu Fang, G. Han, Fei Zhou, Dong Li, Hong-da Chen, Wei Gu","doi":"10.3724/SP.J.1008.2015.00065","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.00065","url":null,"abstract":"","PeriodicalId":6893,"journal":{"name":"Academic Journal of Second Military Medical University","volume":"20 1","pages":"65"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69842660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Vav3 gene inhibition on inhibitor of apoptosis proteins and multidrug resistance in gastric cancer cells","authors":"B. Tan, L. Yong, L. Fan, Qun Zhao, Dong Wang, Yu Liu","doi":"10.3724/SP.J.1008.2015.00136","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.00136","url":null,"abstract":"Objective To explore the role of Vav3 gene in multidrug resistance(MDR)of gastric cancer and the related mechanism.Methods QRT-PCR was used to examine the expressions of Vav3 gene in gastric cancer tissues,tumor-adjacent tissues,human gastric cancer cell line SGC7901,and gastric epithelial cell line GES-1.Then Vav3-siRNA was synthesized and tansfected into SGC7901 cells. MTT assay was then used to determine the inhibition rates of tumor cells exposed to chemotherapeutic agents(5-FU,L-OHP)before and after Vav3-siRNA transfection.Real-time RT-PCR and Western blotting analysis were used to observe the expressions of inhibitor of apoptosis proteins(IAPs):xIAP,Survivin,and Livin;meanwhile,the expression and activity of Caspase-3and Caspase-8 were also determined.Results Vav3 was over-expressed in gastric cancer tissues and gastric cell line compared with those in tumor-adjacent tissues and gastric epithelial cell line GES-1(P0.05).Expression of Vav3 was significantly inhibited by Vav3-siRNA(P0.01).Inhibition rates of tumor cells exposed to 5-FU and L-OHP were significantly increased 48 hafter Vav3-siRNA tansfection(P0.05).The expressions of xIAP and Survivin were significantly decreased in cancer cells after Vav3-siRNA tansfection(both P0.05),and no notable change was found for Livin expression;also the expression and activity of Caspase-3and Caspase-8protein were significantly increased after Vav3-siRNA tansfection in SGC7901cells(all P0.05).Conclusion Vav3 can participate in MDR of gastric cancer by regulating apoptotic pathways,and inhibition of Vav3 can help reverse MDR of gastric cancer cells by regulating some IAPs.","PeriodicalId":6893,"journal":{"name":"Academic Journal of Second Military Medical University","volume":"36 1","pages":"136"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69844428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endoscopic resection of third ventricular colloid cysts: a case report","authors":"S. Deng, Jing-hui Li, Tao Sun, Hua-lin Yu, Yi-liu Ma, Ying Yang","doi":"10.3724/SP.J.1008.2015.00115","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.00115","url":null,"abstract":"","PeriodicalId":6893,"journal":{"name":"Academic Journal of Second Military Medical University","volume":"36 1","pages":"115"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69844457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First line XELOX chemotherapy combined with subsequent capecitabine maintenance chemotherapy for patients with advanced gastric cancer: a clinical analysis of effectiveness","authors":"Jing Yang, Mian-hua Wu, Qi Zhang, Xiangcheng Wang","doi":"10.3724/SP.J.1008.2015.00346","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.00346","url":null,"abstract":"","PeriodicalId":6893,"journal":{"name":"Academic Journal of Second Military Medical University","volume":"36 1","pages":"346"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69845938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Junctophilin-2 protein in cardiac myocytes: progress in physiological and pathophysiological functions","authors":"Hua Shen, Miaoling Li, Deng-ke He, Liang Yin, Wang Xi, Zhinong Wang","doi":"10.3724/SP.J.1008.2015.00429","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.00429","url":null,"abstract":"The normal cardiac function relies on highly specialized subcellular architectures.The subcellular domains junctional membrane complexes(JMCs)are essential for excitation-contraction coupling of the myocardium.Junctophilin-2(JPH2)has been widely recognized as the crucial structural protein involved in JMCs.Initial studies limited the role of JPH2 to anchoring junctional sarcoplasmic reticulum(SR)and transverse-tubule(T-tubule) membrane invaginations.Recently,researchers have found an expanded role of JPH2 in the development of postnatal T-tubule in mammals,progression of disease in failing hearts,hypertrophic cardiomyopathy and arrhythmias.In this review we summarized the role of JPH2 in the above physiological or pathophysiological processes and discussed the perspective in future investigation.","PeriodicalId":6893,"journal":{"name":"Academic Journal of Second Military Medical University","volume":"36 1","pages":"429"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69847257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of silk fibroin on degradation and in vivo biocompatibility of poly (L-lactic-co-ε-caprolactone) electronspun nanofibrous scaffolds","authors":"Zixuan Cheng, Cheng-wei Yang, Li Liu, Yang Yang, Guoying Deng, Xiao-Jian Ye","doi":"10.3724/SP.J.1008.2015.00500","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.00500","url":null,"abstract":"Objective To investigate the effect of silk fibroin(SF) on degradation and biocompatibility of poly(L-lactic acidco-e-caprolactone)(P[LLA-CL]) in vivo.Methods The scaffolds of P(LLA-CL)(w / w = 1 ∶ 1) blended with 25% of SF(SF / P[LLA-CL]) and P(LLA-CL) were prepared by electrospinning.Both kinds of scaffolds were subcutaneously implanted in 45 6-monthold rats for up to 6 months to evaluate their degradation and biocompatibility characteristics.Results Pathological sections showed P(LLA-CL) scaffold become swollen and began to separate into different layers after 3 months,and then become broken after 6 months;while SF / P(LLA-CL) scaffold largely maintained its structure after 6 months.Immunohistochemical staining showed a large number of macrophages on the surface and in P(LLA-CL) scaffolds 1 month after implantation,and they could still be found 3 months after implantation,accompanied by foreign body giant cells; while no obvious macrophages or foreign body giant cells were found in SF / P(LLA-CL) scaffolds at different time points.Examination of inflammatory gene expression showed that TNF-α and IL-10 expression in P(LLA-CL) scaffolds was significantly higher than that in SF / P(LLA-CL) scaffolds 1 week after implantation(P 0.05),the same was also true for TNF-α,IL-1β and IL-10 expression 1 month after implantation(P 0.05),for TNF-α and IL-10 expression 2 months after implantation(P 0.05),for TGF-β expression 3 months after implantation(P 0.05),and for IL-1β and TGF-β expression 6months after implantation(P 0.05).Conclusion SF incorporation can delay degradation,reduce inflammation,and improve the biocompatibility of P(LLA-CL) scaffolds,which may provide reference for scaffold design in tissue engineering.","PeriodicalId":6893,"journal":{"name":"Academic Journal of Second Military Medical University","volume":"36 1","pages":"500"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69847742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prostate biopsy guided by transrectal ultrasound combined with MRI compartmental localization in diagnosis of prostate cancer","authors":"Hui-qing Wang, Guang’an Xiao, Bo Yang, Guodong Jing, Chao Zhang, F. Guo, Yinghao Sun","doi":"10.3724/SP.J.1008.2015.00522","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.00522","url":null,"abstract":"Objective To explore the value of prostate biopsy guided by transrectal ultrasound combined with MRI compartmental localization in the diagnosis of prostate cancer.Methods Fifty patients with elevated prostate specific antigen levels were recruited in this study.All the patients underwent MRI scan before biopsy and the prostate was divided into 12 compartments accordingly.The direction and region of the division were consistent with the ultrasound.Systematic 12-samples needle biopsy was performed and additional biopsy was done for the suspected compartments.According to histopathology,the positive detection rates of systematic 12-samples needle biopsy and systematic biopsy combined with added punctures of suspected compartments were analyzed and compared.Results Prostate cancer detection rate of systematic biopsy combined with punctures of suspected compartment was significantly higher than that of systematic 12-samples needle biopsy(42.0% vs 36.0%,P 0.05).Systematic biopsy combined with punctures of suspected compartment caused hematuria in 5 cases,bloody stools in 13 cases,and fever in one,and there were no other complications.Conclusion Transrectal ultrasound combined with MRI compartmental localization can improve the outcome of prostate biopsy,benefiting the early diagnosis of prostate cancer.","PeriodicalId":6893,"journal":{"name":"Academic Journal of Second Military Medical University","volume":"12 1","pages":"522"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69848112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}