IF 2.7

血液科学(英文) Pub Date : 2026-03-02 eCollection Date: 2026-03-01 DOI: 10.1097/BS9.0000000000000283

Sha Hao, Changya Chen

引用次数: 0

Anti-thymocyte globulin-resistant CD4⁺ memory T cells contribute to haplo-fever after allogeneic hematopoietic stem cell transplantation. 抗胸腺细胞球蛋白抵抗CD4+记忆T细胞有助于异基因造血干细胞移植后单倍体发热。

IF 2.7

血液科学(英文) Pub Date : 2026-02-26 eCollection Date: 2026-03-01 DOI: 10.1097/BS9.0000000000000280

Huihong Huang, Ting Lu, Honglin Duan, Jingtao Huang, Quan Gu, Hui Cheng, Zengkai Pan, Xiaoxia Hu

{"title":"Anti-thymocyte globulin-resistant CD4+ memory T cells contribute to haplo-fever after allogeneic hematopoietic stem cell transplantation.","authors":"Huihong Huang, Ting Lu, Honglin Duan, Jingtao Huang, Quan Gu, Hui Cheng, Zengkai Pan, Xiaoxia Hu","doi":"10.1097/BS9.0000000000000280","DOIUrl":"https://doi.org/10.1097/BS9.0000000000000280","url":null,"abstract":"Haplo-fever (HF) is a form of non-infectious fever that occurs shortly after haploidentical (HID) hematopoietic stem cell transplantation (HSCT). Its clinical significance, risk factors, and pathophysiology, particularly in the context of anti-thymocyte globulin (ATG)-based prophylaxis for graft-versus-host disease (GvHD), remain largely unknown. Here, we retrospectively analyzed clinical data from 143 consecutive HID HSCT recipients. Patients with HF exhibited a higher incidence of chronic GvHD, a lower risk of measurable residual disease recurrence, and improved event-free survival, particularly among those receiving ATG-based acute GvHD prophylaxis. Identified risk factors for HF included higher infused doses of CD34+ cells, mononucleated cells, CD3+ T cells, and CD4+ memory T cells. At 90 days post-transplant, patients with HF demonstrated elevated absolute numbers of CD4+ memory T cells and activated CD4+ and CD8+ T cells. RNA sequencing of peripheral blood CD3+ T cells at day 2 post-transplant revealed transcriptional signatures of T-cell activation and an abundance of activated CD4+ memory T cells as hallmarks of HF. Using multiple complementary approaches, we demonstrated the impact of HF on transplant outcomes, identified its risk factors, and elucidated the underlying cellular and molecular mechanisms.","PeriodicalId":67343,"journal":{"name":"血液科学(英文)","volume":"8 1","pages":"e00280"},"PeriodicalIF":2.7,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12947993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147327938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A pipeline for single-cell chromatin accessibility data analysis. 单细胞染色质可及性数据分析管道。

IF 2.7

血液科学(英文) Pub Date : 2026-02-25 eCollection Date: 2026-03-01 DOI: 10.1097/BS9.0000000000000259

Mengke Zhang, Changya Chen

引用次数: 0

Erratum: Super-resolution imaging informed scRNA sequencing analysis reveals the critical role of GDF15 in rejuvenating aged hematopoietic stem cells. 勘误：超分辨率成像告知scRNA测序分析揭示了GDF15在衰老造血干细胞中恢复活力的关键作用。

IF 2.7

血液科学(英文) Pub Date : 2025-12-08 eCollection Date: 2025-12-01 DOI: 10.1097/BS9.0000000000000265

Zhongyu Shi, Xuefei Zhang, Huihui Yang, Xiaolu Zheng, Mengxiao Niu, Yongjian Zhang, Pengfei Yuan, Wensheng Wei, Gang Huang, Riguo Fang, Liangyi Chen

引用次数: 0

FLT3-ITD with NPM1 and/or DNMT3A co-mutations in acute myeloid leukemia: prognostic significance and the role of maintenance therapy post-transplantation. 急性髓系白血病FLT3-ITD伴NPM1和/或DNMT3A共突变：移植后维持治疗的预后意义和作用

IF 2.7

血液科学(英文) Pub Date : 2025-12-05 eCollection Date: 2025-12-01 DOI: 10.1097/BS9.0000000000000267

Ruixin Li, Jiaxin Cao, Mingyang Wang, Jinting Fan, Yang Yang, Hongye Gao, Fengjiao Wang, Donglin Yang, Rongli Zhang, Weihua Zhai, Yigeng Cao, Jialin Wei, Aiming Pang, Yi He, Sizhou Feng, Mingzhe Han, Erlie Jiang

{"title":"FLT3-ITD with NPM1 and/or DNMT3A co-mutations in acute myeloid leukemia: prognostic significance and the role of maintenance therapy post-transplantation.","authors":"Ruixin Li, Jiaxin Cao, Mingyang Wang, Jinting Fan, Yang Yang, Hongye Gao, Fengjiao Wang, Donglin Yang, Rongli Zhang, Weihua Zhai, Yigeng Cao, Jialin Wei, Aiming Pang, Yi He, Sizhou Feng, Mingzhe Han, Erlie Jiang","doi":"10.1097/BS9.0000000000000267","DOIUrl":"10.1097/BS9.0000000000000267","url":null,"abstract":"FLT3-ITD, NPM1, and DNMT3A mutations are common in acute myeloid leukemia (AML). However, the prognostic role of FLT3-ITD combined with NPM1 and/or DNMT3A mutations after allogeneic hematopoietic stem cell transplantation (allo-HSCT) remains unclear. In this retrospective study, 100 AML patients were selected from a cohort of 1292 who underwent allo-HSCT between 2014 and 2024. Patients were stratified by co-mutation profiles to compare prognosis, identify predictors of survival and relapse, and assess the efficacy of maintenance therapy. With a median follow-up after allo-HSCT of 16.1 months (interquartile range 8.1-26.2), 2-year overall survival (OS) rates were 65.1%, 68.3%, and 67.1%; leukemia-free survival (LFS) rates were 61.6%, 68.7%, and 63.2%; and cumulative incidence of relapse (CIR) rates were 16.9%, 12.5%, and 15.8%, respectively. No significant differences were observed among the groups. In multivariate analysis with FLT3 inhibitor as a time-dependent covariate, FLT3-ITD measurable residual disease (MRD) positivity prior to allo-HSCT was independently associated with inferior OS (hazard ratio [HR] = 3.51, 95% CI 1.34-9.17), LFS (HR = 3.05, 95% CI 1.26-7.35), and CIR (HR = 4.78, 95% CI 1.55-14.81). In contrast, posttransplant maintenance therapy with FLT3 inhibitors independently conferred a favorable impact on OS (HR = 0.15, 95% CI 0.03-0.66), LFS (HR = 0.24, 95% CI 0.07-0.83), CIR (HR = 0.10, 95% CI 0.01-0.66), and nonrelapse mortality (NRM) (HR = 0.25, 95% CI 0.07-0.89). In conclusion, FLT3-ITD-based double or triple mutations showed comparable posttransplant outcomes. FLT3-ITD MRD status and early maintenance therapy were key prognostic and therapeutic factors.","PeriodicalId":67343,"journal":{"name":"血液科学(英文)","volume":"7 4","pages":"e00267"},"PeriodicalIF":2.7,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12685400/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145717040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dissecting cellular ecosystem with single-cell CRISPR screens. 用单细胞CRISPR屏幕解剖细胞生态系统。

IF 2.7

血液科学(英文) Pub Date : 2025-12-04 eCollection Date: 2025-12-01 DOI: 10.1097/BS9.0000000000000266

Zhirui Liu, Zilin Lan, Xiaoli Kang, Yao Yao, Shuquan Rao

{"title":"Dissecting cellular ecosystem with single-cell CRISPR screens.","authors":"Zhirui Liu, Zilin Lan, Xiaoli Kang, Yao Yao, Shuquan Rao","doi":"10.1097/BS9.0000000000000266","DOIUrl":"10.1097/BS9.0000000000000266","url":null,"abstract":"Clustered regularly interspaced short palindromic repeats (CRISPR) screens represent a transformative force in biological discovery, enabling the unbiased interrogation of gene function in a wide range of applications. Traditional screening approaches predominantly hinge on cell fitness or established markers, which inherently constrain their abilities for unbiased biological discovery. By contrast, single-cell CRISPR screening technologies, which combine pooled CRISPR screens with an array of sophisticated single-cell omics platforms, permit comprehensive profiling of the transcriptome and epigenome following individual genetic manipulations within complex cellular ecosystems. Over the past decade, a panoply of single-cell CRISPR platforms has emerged, each tailored to address specific experimental challenges. Iterative refinements in protocols have bolstered precision, scalability, and reproducibility, thereby enormously advancing functional genomics and translational research. However, technical obstacles such as perturbation efficiency, scalability, and data integration persist, necessitating cross-disciplinary collaboration and innovation. As single-cell CRISPR platforms evolve to incorporate spatial resolution, multi-omics integration, and AI-guided design, they are poised to bridge the gap between genetic perturbation and system-level interpretation. Here, we summarize recent advances in single-cell CRISPR technologies, outline their applications, and provide a comparative framework to guide platform selection (Perturb-seq, CROP-seq, ECCITE-seq, Direct-seq, and Mosaic-seq).","PeriodicalId":67343,"journal":{"name":"血液科学(英文)","volume":"7 4","pages":"e00266"},"PeriodicalIF":2.7,"publicationDate":"2025-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12680425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145703089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

T Cell immune reconstitution after allo-HSCT: key factors and implications. 同种异体造血干细胞移植后T细胞免疫重建：关键因素及其意义。

IF 2.7

血液科学(英文) Pub Date : 2025-12-01 DOI: 10.1097/BS9.0000000000000261

Weiqian Dai, Fang Dong, Tao Cheng, Sha Hao

{"title":"T Cell immune reconstitution after allo-HSCT: key factors and implications.","authors":"Weiqian Dai, Fang Dong, Tao Cheng, Sha Hao","doi":"10.1097/BS9.0000000000000261","DOIUrl":"10.1097/BS9.0000000000000261","url":null,"abstract":"Allogeneic hematopoietic stem cell transplantation (allo-HSCT) represents a curative therapy for hematological malignancies, with T-cell immune reconstitution playing a pivotal role in determining clinical outcomes. This review comprehensively illustrates the processes and influencing factors of T-cell recovery post-HSCT, highlighting the dual pathways of reconstitution: thymus-independent peripheral expansion and thymus-dependent central regeneration. Key factors such as recipient and donor age, human leukocyte antigen disparity, conditioning regimens, immunosuppressive therapies, cytomegalovirus reactivation, and graft-versus-host disease (GVHD) significantly impact T-cell reconstitution dynamics and functional recovery. Furthermore, the article discusses the critical balance between graft-versus-leukemia (GVL) effects and GVHD, emphasizing how T-cell exhaustion, inhibitory receptor overexpression, and clonal dynamics contribute to relapse. Emerging technologies, including single-cell multi-omics, spatially resolved proteomics, T cell receptor repertoire analysis, and artificial intelligence-driven modeling, are explored for their potential to deepen mechanistic understanding and enable personalized therapeutic strategies. Ultimately, enhancing T-cell reconstitution through optimized transplantation protocols and targeted interventions is essential for reducing complications and improving long-term survival.","PeriodicalId":67343,"journal":{"name":"血液科学(英文)","volume":"7 4","pages":"e00261"},"PeriodicalIF":2.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12672197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145679431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Large-scale proteomic and phosphoproteomic analysis of erythroid enucleation and maturation. 红系去核和成熟的大规模蛋白质组学和磷酸化蛋白质组学分析。

IF 2.7

血液科学(英文) Pub Date : 2025-11-26 eCollection Date: 2025-12-01 DOI: 10.1097/BS9.0000000000000263

Yuanliang Peng, Liming Zhu, Min Duan, Jing Liu

引用次数: 0

Single-cell transcriptomic dissection of two waves of endothelial-hematopoietic transition in a murine yolk sac. 小鼠卵黄囊内皮-造血转变两波的单细胞转录组学解剖。

IF 2.7

血液科学(英文) Pub Date : 2025-11-24 eCollection Date: 2025-12-01 DOI: 10.1097/BS9.0000000000000260

Zhe Chen, Chaojie Wang, Xupeng Chen, Yang Yang, Yandong Gong, Yingpeng Yao, Yanli Ni, Zongcheng Li, Bing Liu, Yu Lan

{"title":"Single-cell transcriptomic dissection of two waves of endothelial-hematopoietic transition in a murine yolk sac.","authors":"Zhe Chen, Chaojie Wang, Xupeng Chen, Yang Yang, Yandong Gong, Yingpeng Yao, Yanli Ni, Zongcheng Li, Bing Liu, Yu Lan","doi":"10.1097/BS9.0000000000000260","DOIUrl":"https://doi.org/10.1097/BS9.0000000000000260","url":null,"abstract":"The yolk sac drives vertebrate embryonic hematopoiesis through primitive hematopoiesis and endothelial-to-hematopoietic transition (EHT) waves. However, dynamic cellular and molecular changes during EHT of the yolk sac remain to be elucidated. We built a comprehensive atlas of early endothelial and hematopoietic development in the yolk sac by integrating single-cell transcriptomic data from mouse embryos (E6.75-E11.0). Focusing on the yolk sac (E7.5-E9.5), we established a refined atlas capturing key cell populations of EHT in the yolk sac. This enabled the identification of distinct hemogenic endothelial cell (HEC) subpopulations and revealed 2 fundamentally distinct waves of yolk sac hemogenesis via EHT that differed in temporal emergence, cellular origin, molecular signature, and lineage bias. The first EHT wave, emerging around E8.0, originated from primordial endothelial cells and exhibited a bias toward the generation of erythromyeloid progenitors. In contrast, the second EHT wave, emerging around E8.5, originated from maturing yolk sac endothelial cells, expressed key intraembryonic HEC markers (Hlf, Nupr1, Gfi1), and showed a hematopoietic stem and progenitor cell fate bias. Furthermore, molecular dynamics analysis of the pseudo-trajectory during the 2 waves of EHT in mouse yolk sacs revealed different dynamic changes in several pathways, particularly the ribosome and metabolic pathways. The yolk sac endothelial and hematopoietic atlas is accessible from an interactive web server (https://lllab.shinyapps.io/ysshinyapp/). Collectively, this study provides novel insights into the multi-wave nature of yolk sac hematopoiesis, clarifies the fundamental principles of yolk sac EHT at a single-cell resolution, and offers potential guidance for in vitro blood cell regeneration strategies.","PeriodicalId":67343,"journal":{"name":"血液科学(英文)","volume":"7 4","pages":"e00260"},"PeriodicalIF":2.7,"publicationDate":"2025-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12657049/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145643504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Late-onset radiation-induced brain necrosis following allogeneic transplantation for CNS-involved acute myeloid leukemia after whole brain irradiation: two case reports. 全脑放射治疗累及中枢神经系统的急性髓性白血病异体移植后迟发性脑坏死2例报告

IF 2.7

血液科学(英文) Pub Date : 2025-11-24 eCollection Date: 2025-12-01 DOI: 10.1097/BS9.0000000000000264

Jotaro Yamamoto, Shinsuke Takagi, Yuki Shimizu, Takeshi Fujii, Mika Kuno, Otoya Watanabe, Kyosuke Yamaguchi, Kosei Kageyama, Daisuke Kaji, Shinji Ito, Yuki Taya, Aya Nishida, Hisashi Yamamoto, Go Yamamoto, Yuki Asano-Mori, Atsushi Wake, Shuichi Taniguchi, Naoyuki Uchida

引用次数: 0

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