Journal of Natural Medicines最新文献

{"title":"Alliaxylines A–E: five new mexicanolides from the stem barks of Dysoxylum alliaceum (Blume) Blume ex A.Juss","authors":"Sandra Amalia Riyadi,&nbsp;Al Arofatus Naini,&nbsp;Tri Mayanti,&nbsp;Ronny Lesmana,&nbsp;Mohamad Nurul Azmi,&nbsp;Sofa Fajriah,&nbsp;Siriporn Jungsuttiwong,&nbsp;Unang Supratman","doi":"10.1007/s11418-024-01794-2","DOIUrl":"10.1007/s11418-024-01794-2","url":null,"abstract":"<div><p>A total of five new mexicanolides (<b>1</b>–<b>5</b>), namely alliaxylines A–E, together with two known limonoids <b>6</b> and <b>7</b>, were isolated and identified from <i>Dysoxylum alliaceum</i> (Blume) Blume ex. A.Juss. (Meliaceae). The structures of these compounds were elucidated based on extensive spectroscopic analyses, including HR-ESI–MS, UV, IR, 1D, and 2D NMR, as well as theoretical stimulation of NMR shifts with the DP4 + algorithm. Consequently, this study aimed to examine cytotoxic activities of these compounds against MCF-7 and A549 cell lines. The results implied that compound <b>2</b> was the most potent against the two tested cells, with IC₅₀ values of 34.95 ± 0.21 and 44.39 ± 1.03 µM.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"78 3","pages":"558 - 567"},"PeriodicalIF":2.5,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140189336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new 3,4-seco-isopimarane and three new isopimarane diterpenoids from Kaempferia champasakensis collected from Vietnam and their cytotoxic activities","authors":"Kiep Minh Do,&nbsp;Shotaro Hoshino,&nbsp;Takeshi Kodama,&nbsp;Hien Minh Nguyen,&nbsp;Son Van Le,&nbsp;Naotaka Ikumi,&nbsp;Hiroyasu Onaka,&nbsp;Hiroyuki Morita","doi":"10.1007/s11418-024-01789-z","DOIUrl":"10.1007/s11418-024-01789-z","url":null,"abstract":"<div><p>A phytochemical investigation of <i>Kaempferia champasakensis</i> rhizomes led to the isolation of a new 3,4-seco-isopimarane diterpene, kaempferiol A (<b>1</b>), and three new isopimarane diterpenes, kaempferiols B–D (<b>2</b>–<b>4)</b>, together with six known isopimarane diterpenes (<b>5–10)</b>. The structures of <b>1</b>–<b>4</b> were elucidated by extensive spectroscopic analyses, including HR-ESI–MS, UV, IR, and 1D and 2D NMR. The absolute configurations of <b>1</b>,<b> 3</b>, and<b> 4</b> were determined by ECD calculations, while that of <b>2</b> was established using the modified Mosher method. All isolated compounds were tested for cytotoxicity against three human cancer cell lines, lung cancer (A549), cervical cancer (HeLa), and breast cancer (MCF-7). Among them, <b>6</b> and <b>7</b> showed moderate cytotoxic activities against the three tested cell lines, with IC₅₀ values ranging from 38.04 to 27.77 μM, respectively.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"78 3","pages":"537 - 546"},"PeriodicalIF":2.5,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140189311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-enzymatic reactions in biogenesis of fungal natural products","authors":"Shinji Kishimoto","doi":"10.1007/s11418-024-01797-z","DOIUrl":"10.1007/s11418-024-01797-z","url":null,"abstract":"<div><p>Fungi have long been regarded as abundant sources of natural products (NPs) exhibiting significant biological activities. Decades of studies on the biosynthesis of fungal NPs revealed that most of the biosynthetic steps are catalyzed by sophisticated enzymes encoded in biosynthetic gene clusters, whereas some reactions proceed without enzymes. These non-enzymatic reactions complicate biosynthetic analysis of NPs and play important roles in diversifying the structure of the products. Therefore, knowledge on the non-enzymatic reactions is important for elucidating the biosynthetic mechanism. This review focuses on non-enzymatic reactions we recently encountered during biosynthetic studies of four types of NPs (viridicatins, Sch210972, lentopeptins, and lentofuranine).</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"78 3","pages":"467 - 473"},"PeriodicalIF":2.5,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11101550/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140189337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory effects of senkyuchachosan on SARS-CoV-2 papain-like protease activity in vitro","authors":"Yuka Kiba,&nbsp;Takashi Tanikawa,&nbsp;Tsuyoshi Hayashi,&nbsp;Takami Yokogawa,&nbsp;Aiko Sano,&nbsp;Ryuichiro Suzuki,&nbsp;Masashi Kitamura","doi":"10.1007/s11418-024-01788-0","DOIUrl":"10.1007/s11418-024-01788-0","url":null,"abstract":"<p>Papain-like protease (PLpro) enzyme plays a vital role in viral replication as it breaks down polyproteins and disrupts the host's immune response. There are few reports on Kampo formulas that focus on PLpro activity. In this study, we evaluated the inhibitory effects of senkyuchachosan, a traditional Japanese medicine, on PLpro of SARS-CoV-2, the virus responsible for causing COVID-19. We purified the PLpro enzyme and conducted in vitro enzymatic assays using specific substrates. Among the nine crude drugs present in senkyuchachosan, four (Cyperi Rhizoma, Schizonepetae Spica, Menthae Herba, and Camelliae sinensis Folium [CsF]) strongly inhibited PLpro activity. CsF, derived from <i>Camellia sinensis</i> (green tea), contains polyphenols, including catechins and tannins. To confirm that the PLpro inhibitory effects of senkyuchachosan predominantly stem from tannins, the tannins were removed from the decoction using polyvinylpolypyrrolidone (PVPP). The inhibitory effect of senkyuchachosan on PLpro activity was reduced by the removal of PVPP. In addition, the tannin fraction obtained from the CsF extracts showed significant PLpro inhibitory effects. These findings lay the groundwork for the potential development of therapeutic agents that target SARS-CoV-2 infection by intervening in proteolytic cleavage of the virus.</p>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"78 3","pages":"784 - 791"},"PeriodicalIF":2.5,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140183433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent trends in ginseng research","authors":"Honoka Ito,&nbsp;Michiho Ito","doi":"10.1007/s11418-024-01792-4","DOIUrl":"10.1007/s11418-024-01792-4","url":null,"abstract":"<div><p>Ginseng, the dried root of <i>Panax ginseng</i>, contains ginsenosides and has long been used in Korea, China, and Japan to treat various symptoms. Many studies on the utility of ginseng have been conducted and in this paper we investigate recent trends in ginseng research. <i>P. ginseng</i> studies were collected from scientific databases (PubMed, Web of Science, and SciFinderⁿ) using the keywords “<i>Panax ginseng</i> C.A. Meyer”, “ginsenosides”, “genetic diversity”, “biosynthesis”, “cultivation”, and “pharmacology”. We identified 1208 studies up to and including September 2023: 549 studies on pharmacology, 262 studies on chemical components, 131 studies on molecular biology, 58 studies on cultivation, 71 studies on tissue culture, 28 studies on clinical trials, 123 reviews, and 49 studies in other fields. Many researchers focused on the characteristic ginseng component ginsenoside to elucidate the mechanism of ginseng’s pharmacological action, the relationship between component patterns and cultivation areas and conditions, and gene expression.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"78 3","pages":"455 - 466"},"PeriodicalIF":2.5,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140183434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoreaction products of extract from the fruiting bodies of Polyozellus multiplex","authors":"Hayato Otsuka,&nbsp;Keiyo Nakai,&nbsp;Emi Shimizu,&nbsp;Takamasa Yamaguchi,&nbsp;Mitsuhisa Yamano,&nbsp;Hiroaki Sasaki,&nbsp;Kiyotaka Koyama,&nbsp;Kaoru Kinoshita","doi":"10.1007/s11418-024-01790-6","DOIUrl":"10.1007/s11418-024-01790-6","url":null,"abstract":"<div><p>Photochemical reactions are powerful tools for synthesizing organic molecules. The input of energy provided by light offers a means to produce strained and unique molecules that cannot be assembled using thermal protocols, allowing for the production of immense molecular complexity in a single chemical step. Furthermore, unlike thermal reactions, photochemical reactions do not require active reagents such as acids, bases, metals, or enzymes. Photochemical reactions play a central role in green chemistry. This article reports the isolation and structure determination of four new compounds (<b>1</b>–<b>4</b>) from the photoreaction products of the <i>Polyozellus multiplex</i> MeOH ext. The structures of the new compounds were elucidated using MS, IR, comprehensive NMR measurements and microED. The four compounds were formed by deacetylation of polyozellin, the main secondary metabolite of <i>P. multiplex</i>, and addition of singlet oxygen generated by sunlight. To develop drugs for treating Alzheimer’s disease (AD) on the basis of the amyloid cascade hypothesis, the compounds (<b>1</b>–<b>4</b>) obtained by photoreaction were evaluated for BACE1 inhibitory activity. The hydrolysates (<b>5</b> and <b>6</b>) of polyozellin, the main secondary metabolites of <i>P. multiplex</i>, were also evaluated. The photoreaction products (<b>3</b> and <b>4</b>) and hydrolysates (<b>5</b> and <b>6</b>) of polyozellin showed BACE1 inhibitory activity (IC₅₀: 2.2, 16.4, 23.3, and 5.3 μM, respectively).</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"78 3","pages":"547 - 557"},"PeriodicalIF":2.5,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140178978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resultant compound from sublimation test for Gentianae Radix in Japanese Pharmacopoeia was 5-(hydroxymethyl)furfural","authors":"Atsushi Tsuge,&nbsp;Yuto Goto,&nbsp;Sayaka Masada,&nbsp;Michiho Ito","doi":"10.1007/s11418-024-01802-5","DOIUrl":"10.1007/s11418-024-01802-5","url":null,"abstract":"<div><p>Gentianae Radix, an herbal medicine, has been used as a gastrointestinal drug in Japan. In the Japanese Pharmacopoeia 18th Revision, the sublimation test is specified as an identification test for Gentianae Radix. The compound obtained in this sublimation test was believed to be gentisin, a xanthone family compound. However, the compound we identified using liquid chromatography-high-resolution mass spectrometry (LC-HRMS) and ¹H- and ¹³C-NMR was 5-(hydroxymethyl)furfural (5-HMF). The same compound was found to be a sublimate of Gentianae Scabrae Radix and Gentianae Macrophyllae Radix, belonging to the same genus as Gentianae Radix. These results indicate the necessity to revise the identification test for Gentianae Radix to a more unique method.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"78 3","pages":"799 - 802"},"PeriodicalIF":2.5,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140168651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The protective effects of Ninjin’yoeito against liver steatosis/fibrosis in a non-alcoholic steatohepatitis model mouse","authors":"Kyohei Takano,&nbsp;Marisa Kaneda,&nbsp;Yayoi Aoki,&nbsp;Nina Fujita,&nbsp;Shigeki Chiba,&nbsp;Seiwa Michihara,&nbsp;Li-Kun Han,&nbsp;Ryuji Takahashi","doi":"10.1007/s11418-024-01786-2","DOIUrl":"10.1007/s11418-024-01786-2","url":null,"abstract":"<div><p>Non-alcoholic steatohepatitis (NASH) is a progressive fibrotic form of non-alcoholic fatty liver disease. Liver fibrosis leads to liver cancer and cirrhosis, and drug therapy for NASH remains lacking. Ninjin’yoeito (NYT) has shown antifibrotic effects in a model of liver fibrosis without steatosis but has not been studied for NASH. Therefore, we evaluated the efficacy of NYT in mice fed a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) as a NASH model. Compared with the normal diet group, mice fed CDAHFD showed decreased body weight and increased white adipose tissue, liver weight, and triglyceride content in the liver. Furthermore, a substantial increase in the hepatic concentration of hydroxyproline, expression of α-smooth muscle actin (<i>α-SMA</i>), and transforming growth factor-β was observed in CDAHFD-fed mice. Masson’s trichrome and Picro-Sirius red staining revealed a remarkable increase in collagen fiber compared with the normal diet group. Compared with mice that received CDAHFD alone, those supplemented with NYT exhibited reduced hepatic triglyceride and hydroxyproline levels and <i>α-SMA</i> expression. Additionally, compared with the group fed CDAHFD alone, the stained liver tissues of NYT-treated mice exhibited a reduction in Masson’s trichrome- and Picro-Sirius red-positive areas. Locomotor activity was significantly reduced in the CDAHFD-fed group compared with the normal diet group. In the NYT-treated group, the CDAHFD-induced decrease in locomotor activity was significantly suppressed. The findings indicate that NYT inhibited fatty and fibrotic changes in the livers of NASH mice and alleviated the decrease in locomotor activity. Therefore, NYT may serve as a novel therapeutic approach for NASH.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"78 3","pages":"514 - 524"},"PeriodicalIF":2.5,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11418-024-01786-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140148546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Four new resin glycosides from Ipomoea muricata seeds: muricatins XIV–XVII","authors":"Masateru Ono,&nbsp;Daiki Tenmaya,&nbsp;Maki Tarumi,&nbsp;Syouri Satou,&nbsp;Kotone Tsuji,&nbsp;Hirotaka Nishikawa,&nbsp;Shin Yasuda,&nbsp;Hiroyuki Miyashita,&nbsp;Jian-Rong Zhou,&nbsp;Kazumi Yokomizo,&nbsp;Hitoshi Yoshimitsu,&nbsp;Ryota Tsuchihashi,&nbsp;Masafumi Okawa,&nbsp;Junei Kinjo","doi":"10.1007/s11418-024-01787-1","DOIUrl":"10.1007/s11418-024-01787-1","url":null,"abstract":"<div><p><i>Ipomoea muricata</i> (L.) Jacq. seeds (Convolvulaceae) are used as a traditional laxative and carminative medicine. Muricatins XIV (<b>1</b>), XV (<b>2</b>), XVI (<b>3</b>), and XVII (<b>4</b>), were isolated from<i> I. muricata</i> seeds as four new resin glycosides, along with seven known compounds, three of which were isolated for the first time as natural products; their structures were determined using MS and NMR spectroscopy. Compounds <b>1</b>–<b>4</b> are macrolactones (jalapins); the sugar moieties of <b>1</b>, <b>2</b>, and <b>4</b> are partially acylated with 2<i>S</i>-methylbutyric acid, while that of <b>3</b> is esterified with 2<i>S</i>-methylbutyric and 2<i>S</i>-methyl-3<i>S</i>-hydroxybutyric acids. In addition, the antiviral activities of the seven compounds obtained in this study, together with five known compounds obtained in our previous study into resin glycosides from <i>I. muricata</i> seeds, were evaluated against herpes simplex virus type 1 (HSV-1); their cytotoxicities against HL-60 human promyelocytic leukemia cells were also investigated. All examined jalapins exhibited similar or slightly weaker anti-HSV-1 activities than acyclovir, the positive control; however, the glycosidic acid of <b>4</b> was inactive, while its methyl ester was weakly active. On the other hand, cytotoxicity testing against HL-60 cells showed similar results to those observed during anti-HSV-1 activity testing, with the exception that one jalapin was less active.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"78 3","pages":"525 - 536"},"PeriodicalIF":2.5,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140058389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protopanaxadiol improves lupus nephritis by regulating the PTX3/MAPK/ERK1/2 pathway","authors":"Zhenyuan Li,&nbsp;Hailin Gan,&nbsp;Kai Ji,&nbsp;Mingyan Yang,&nbsp;Tao Pan,&nbsp;Xiangting Meng,&nbsp;Teng Liu,&nbsp;Zhixia Wang,&nbsp;Baifang Gong,&nbsp;Ke Liu,&nbsp;Dong Qi,&nbsp;Huaying Fan","doi":"10.1007/s11418-023-01777-9","DOIUrl":"10.1007/s11418-023-01777-9","url":null,"abstract":"<div><p>Lupus nephritis (LN) is a kidney disease that occurs after systemic lupus erythematosus (SLE) affects the kidneys. Pentraxin 3 (PTX3) is highly expressed in the serum of patients with LN. Renal PTX3 deposition is directly related to clinical symptoms such as proteinuria and inflammation. The excessive proliferation of mesangial cells (MCs) is one of the representative pathological changes in the progression of LN, which is closely related to its pathogenesis. Protopanaxadiol (PPD) is the main component of ginsenoside metabolism and has not been reported in LN. The aim of this study was to investigate the relationship between PTX3 and mesangial cell proliferation and to evaluate the potential role and mechanism of PPD in improving LN. PTX3 is highly expressed in the kidneys of LN patients and LN mice and is positively correlated with renal pathological indicators, including proteinuria and PCNA. The excessive expression of PTX3 facilitated the proliferation of MCs, facilitated the activation of the MAPK/ERK1/2 signaling pathway, and increased the expression of HIF-1α. Further studies showed that PPD can effectively inhibit the abnormal proliferation of MCs with high expression of PTX3 and significantly improve LN symptoms such as proteinuria in MRL/lpr mice. The mechanism may be related to the inhibition of the PTX3/MAPK/ERK1/2 pathway. In this study, both in vitro, in vivo, and clinical sample results show that PTX3 is involved in the regulation of MCs proliferation and the early occurrence of LN. Natural active compound PPD can improve LN by regulating the PTX3/MAPK/ERK1/2 pathway.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":654,"journal":{"name":"Journal of Natural Medicines","volume":"78 3","pages":"474 - 487"},"PeriodicalIF":2.5,"publicationDate":"2024-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140019569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}