Journal of Molecular Histology最新文献_第6页

Identification of the oncogenic role of centromere protein M in non-small cell lung cancer via CDC20/MYBL2/Wnt signaling pathways 通过CDC20/MYBL2/Wnt信号通路鉴定着丝粒蛋白M在非小细胞肺癌中的致癌作用

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-04-26 DOI: 10.1007/s10735-025-10423-5

Ling Wu, Jun Li, Haoyu Wang, Xu Chang, Qinglong Kong

{"title":"Identification of the oncogenic role of centromere protein M in non-small cell lung cancer via CDC20/MYBL2/Wnt signaling pathways","authors":"Ling Wu, Jun Li, Haoyu Wang, Xu Chang, Qinglong Kong","doi":"10.1007/s10735-025-10423-5","DOIUrl":"10.1007/s10735-025-10423-5","url":null,"abstract":"<div><p>Lung cancer remains the most prevalent carcinoma with a high mortality rate, yet the underlying mechanisms driving pulmonary neoplasia and disease progression are not fully understood. In our study, we conducted a comprehensive analysis of the transcriptome profiles and clinicopathological characteristics of 515 patients diagnosed with non-small cell lung cancer (NSCLC) from the TCGA database. We identified a significant upregulation of centromere protein M (CENPM) in NSCLC tissues, which was positively correlated with poor prognosis. Furthermore, overexpression of CENPM markedly promoted cell proliferation and increased the tumorigenic potential of NSCLC cell lines (A549/NCI-H1299), leading to accelerated tumor progression and reduced survival time in tumor-bearing mice. Mechanistically, CENPM activated the Wnt/β-catenin signaling pathway via the cell division cycle 20 (CDC20)/MYB proto-oncogene-like 2 (MYBL2) axis. Inhibition of either Wnt signaling or the CDC20/MYBL2 axis attenuated the tumorigenic potential and proliferative effects induced by CENPM. Our findings underscore the critical role of CENPM in driving NSCLC development and suggest that CENPM could serve as a novel biomarker for predicting NSCLC progression in clinical settings.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10735-025-10423-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vildagliptin topical ointment: an effective treatment for imiquimod-induced psoriasis in mice 维格列汀外用软膏：对吡喹莫致小鼠牛皮癣的有效治疗

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-04-26 DOI: 10.1007/s10735-025-10416-4

Basma Farooq Ali, Ahmed Rahmah Abu-Raghif, Hayder Ridha-Salman, Ali Jihad Hemid Al-Athari

{"title":"Vildagliptin topical ointment: an effective treatment for imiquimod-induced psoriasis in mice","authors":"Basma Farooq Ali, Ahmed Rahmah Abu-Raghif, Hayder Ridha-Salman, Ali Jihad Hemid Al-Athari","doi":"10.1007/s10735-025-10416-4","DOIUrl":"10.1007/s10735-025-10416-4","url":null,"abstract":"<div><p>Psoriasis is a chronic immune-related dermatosis characterized by inflamed, thickened, brownish-red, peeling skin patches. Vildagliptin is an anti-diabetic drug with novel anti-inflammatory, anti-oxidative, and anti-proliferative activities. This study aimed to assess the anti-psoriatic activity of topical vildagliptin. 40 Swiss albino mice were sorted into five groups, each with 8 animals. The control group obtained no treatment. The induction group obtained imiquimod cream (5%) at a dose of 62.5 mg per day. The vehicle group obtained imiquimod (as did the induction group), accompanied by topical vehicle application. The clobetasol group obtained imiquimod cream (as did the induction group), and two hours later, clobetasol ointment (0.05%) was administered. The vildagliptin group obtained imiquimod (as in the induction group), followed by topical vildagliptin ointment (3%), two hours after induction. The experiment lasts for 8 consecutive days. Evaluations were conducted on the results of biochemical indicators, histological assessments, and clinical observations. Vildagliptin administered topically effectively corrected psoriatic histological irregularities, improved the psoriasis-like skin lesions such as erythema, flacking, and acanthosis, and attenuated the imiquimod-provoked elevations of PASI and Baker’s score. Further, overexpression of inflammatory markers (TNF-α, IL-17 A, IL-23, and IL-22), angiogenic markers (VEGF), oxidative-stress components (MDA and SOD), and proliferative factors (Ki-67) were dramatically mitigated by vildagliptin treatment. Topical vildagliptin has profound anti-psoriatic effects.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: The Sirt1/FOXO signal pathway involves in regulating osteomyelitis progression via modulating mitochondrial dysfunctions and osteogenic differentiation 纠正：Sirt1/FOXO信号通路通过调节线粒体功能障碍和成骨分化参与调节骨髓炎的进展

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-04-25 DOI: 10.1007/s10735-025-10422-6

Runyao Zhang, Nannan Kou, Feifei Liu, Huan Tong, Shaobo Li, Lirong Ren

引用次数: 0

Mechanism of mitochondrial dysfunction on placental trophoblastic cells in intrahepatic cholestasis of pregnancy 妊娠肝内胆汁淤积症中胎盘滋养细胞线粒体功能障碍的机制

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-04-25 DOI: 10.1007/s10735-025-10427-1

Xiaomei Huang, E. Liao, Aixing Chen, Yong Shao

{"title":"Mechanism of mitochondrial dysfunction on placental trophoblastic cells in intrahepatic cholestasis of pregnancy","authors":"Xiaomei Huang, E. Liao, Aixing Chen, Yong Shao","doi":"10.1007/s10735-025-10427-1","DOIUrl":"10.1007/s10735-025-10427-1","url":null,"abstract":"<div><p>Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy-specific disorder characterized by elevated serum bile acids and adverse fetal outcomes. Elevated bile acids can lead to excessive accumulation of reactive oxygen species (ROS) in the placenta, and high ROS levels can cause mitochondrial damage. This study primarily investigates the mechanisms of bile acid-induced mitochondrial dysfunction to provide precise targets for the treatment of ICP. Single-cell sequencing of human placental tissues was conducted to analyze changes in mitochondrial function of ICP placental trophoblasts. An ICP cell model was established using TCA, and the effects of TCA on trophoblast mitochondrial function were observed through detection of ROS, mitochondrial membrane potential, fluorescence confocal microscopy, and other methods. Single-cell sequencing indicated significant impairment of mitochondrial function in ICP placental trophoblasts, and electron microscopy results also suggested severe damage to the mitochondrial structure of ICP placental trophoblasts. Both the morphology and function of mitochondria in the ICP cell model were significantly altered, possibly due to impaired mitochondrial transcription mechanisms mediated by NRF1/PGC-1α pathway. Elevated serum bile acids in ICP pregnant women may lead to mitochondrial damage in placental trophoblasts through the NRF1/PGC-1α pathway, thereby affecting the function of placental trophoblasts.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143871347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nobiletin ameliorates hormone-induced osteoblast apoptosis by modulating JAK2/STAT3 signaling 诺比列素通过调节JAK2/STAT3信号通路改善激素诱导的成骨细胞凋亡

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-04-25 DOI: 10.1007/s10735-025-10424-4

Xiang Li, Yuanzhen Bai, Jia Tong, Guoqing Mao

{"title":"Nobiletin ameliorates hormone-induced osteoblast apoptosis by modulating JAK2/STAT3 signaling","authors":"Xiang Li, Yuanzhen Bai, Jia Tong, Guoqing Mao","doi":"10.1007/s10735-025-10424-4","DOIUrl":"10.1007/s10735-025-10424-4","url":null,"abstract":"<div><p>Our paper aimed to disclose the effects of nobiletin (NOB) on hormone-induced osteoblast apoptosis and potential action mechanism. MC3T3-E1 cells were randomly separated into normal group, glucocorticoid (GC) group, L-NOB group, M-NOB group, H-NOB group, and Colivelin group (Colivelin: JAK2/STAT3 activator). CCK-8 was applied to ascertain the activity of MC3T3-E1 cells. FITC-Annexin V/PI method was applied to measure cell apoptosis. Alkaline phosphatase (ALP) assay kit was applied to measure ALP activity. Enzyme linked immunosorbent assay (ELISA) was implemented to ascertain the levels of IL-6, IL-1β, TNF-α, and ROS. Western blotting was implemented to distinguish the expressions of JAK2/STAT3 pathway proteins. The viability of MC3T3-E1 cells, ALP activities, and bcl-2 protein level were considerably decreased, while the apoptotic rate, the levels of TNF-α, IL-1β, IL-6, ROS, and the expressions of pJAK2/JAK2, pSTAT3/STAT3, Caspase-3, and bax proteins were greatly increased in each group after GC treatment. In comparison with GC group, MC3T3-E1 cell viability, ALP activity, and bcl-2 protein level in the L-NOB group, M-NOB group, and H-NOB group were greatly increased. Conversely, the apoptotic rate, the levels of TNF-α, IL-1β, IL-6, ROS, and the expressions of pJAK2/JAK2, pSTAT3/STAT3, Caspase-3, and bax proteins were markedly reduced. In contrast to H-NOB group, the apoptotic rate, the levels of TNF-α, IL-1β, IL-6, ROS, and the expressions of pJAK2/JAK2, pSTAT3/STAT3, Caspase-3, and bax proteins in Colivelin group were considerably enhanced, while MC3T3-E1 cell viability, ALP activity, and bcl-2 protein level were greatly declined. NOB ameliorates hormone-induced osteoblast apoptosis by reducing JAK2/STAT3 signaling activity.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10735-025-10424-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143871348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Minocycline attenuates TLR-4, NF-kB, TNF-α and COX-2 protein expression after lipopolysaccharide-induced neuroinflammation in the rat medial prefrontal cortex (mPFC) 米诺环素降低脂多糖诱导的大鼠内侧前额叶皮层（mPFC）神经炎症后TLR-4、NF-kB、TNF-α和COX-2蛋白的表达

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-04-25 DOI: 10.1007/s10735-025-10419-1

Entesar Yaseen Abdo Qaid, Zuraidah Abdullah, Rahimah Zakaria, Idris Long

{"title":"Minocycline attenuates TLR-4, NF-kB, TNF-α and COX-2 protein expression after lipopolysaccharide-induced neuroinflammation in the rat medial prefrontal cortex (mPFC)","authors":"Entesar Yaseen Abdo Qaid, Zuraidah Abdullah, Rahimah Zakaria, Idris Long","doi":"10.1007/s10735-025-10419-1","DOIUrl":"10.1007/s10735-025-10419-1","url":null,"abstract":"<div><p>Minocycline has been shown to ameliorate neuroinflammation that was encountered in many neurodegenerative diseases. This study aims to investigate the expression of inflammatory mediators in the rat medial prefrontal cortex (mPFC) after minocycline treatment in a lipopolysaccharide (LPS)- induced neuroinflammation rat model. Adult male Sprague Dawley (SD) rats (N = 50) were divided into 5 groups: (1) control, (2) LPS (5 mg/kg), (3) LPS + minocycline (25 mg/kg), (4) LPS + minocycline (50 mg/kg) and (5) LPS + memantine (10 mg/kg). Intraperitoneal minocycline and memantine were given daily for 14 days, while LPS injection was given once on the 5th day. Western blot and immunohistochemistry were used to assess density and expression of toll-like receptor-4 (TLR-4), nuclear factor kappa-B (NF-kB), tumor necrosis factor (TNF)-α and cyclooxygenase (COX)-2 in the medial prefrontal cortex (mPFC) of rats. Findings displayed that minocycline significantly decreased expression and density of TLR-4, NF-kB, TNF-α and COX-2 proteins that were comparable to memantine in mPFC of SD rat injected with single intraperitoneal LPS. Interestingly, the anti-inflammatory effects of minocycline 50 mg/kg were significantly more than minocycline 25 mg/kg. This study suggested that minocycline can modulate LPS-induced neuroinflammation in dose-dependent manner in the mPFC area. Thus, it is suggested that minocycline can be used as potential preventive-therapeutic drug for neuroinflammatory diseases such as depression and anxiety.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143871345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N6-methyladenosine-modified circ_0000517 promotes non-small cell lung cancer metastasis via miR-1233-3p/CDH6 axis n6 -甲基腺苷修饰的circ_0000517通过miR-1233-3p/CDH6轴促进非小细胞肺癌转移

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-04-25 DOI: 10.1007/s10735-025-10421-7

Weixian Lin, Lifang Huang, Zhu’an Ou, Yiwen Xuan, Daoqi Zhu, Qipeng Zhang, Enwu Xu

{"title":"N6-methyladenosine-modified circ_0000517 promotes non-small cell lung cancer metastasis via miR-1233-3p/CDH6 axis","authors":"Weixian Lin, Lifang Huang, Zhu’an Ou, Yiwen Xuan, Daoqi Zhu, Qipeng Zhang, Enwu Xu","doi":"10.1007/s10735-025-10421-7","DOIUrl":"10.1007/s10735-025-10421-7","url":null,"abstract":"<div><p>Circular RNAs (circRNAs) exhibit dysregulation in non-small cell lung cancer (NSCLC) and regulate the malignant biological behavior of NSCLC. The N6-methyladenosine (m6A) modification of circRNAs plays a critical role in multiple malignant tumors, and their biological relevance in NSCLC is unclear. Herein, this study was conducted to investigate the novel functional mechanism of highly expressed circ_0000517 in NSCLC by developing in vitro experiments. We found that circ_0000517 was upregulated in NSCLC tissues and cells, and that increased circ_0000517 expression was associated with m6A modification. Biologically, silenced circ_0000517 hindered the proliferation, colony formation, migration and invasion of NSCLC cells in vitro, and also suppressed the EMT-related process. Mechanistically, highly expressed circ_0000517 activated CDH6 expression and EMT evolution through sponging miR-1233-3p. Notably, miR-1233-3p had the opposite effect and reversed the promotion effect of circ_0000517 on the malignant biological behavior of NSCLC cells. Our study revealed a promising novel endogenous regulatory network that m6A-modified circ_0000517 accelerated malignant evolution of NSCLC by targeting the miR-1233-3p/CDH6 axis.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143871346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effects of wheat sprout extract on acrylamide-induced toxicity in testis, prostate gland and sperm parameters of rats 小麦芽提取物对丙烯酰胺致大鼠睾丸、前列腺及精子参数毒性的保护作用

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-04-19 DOI: 10.1007/s10735-025-10414-6

Hamid Reza Moradi, Nasrin Kazemipour, Saeed Nazifi, Melika Khodayari, Laleh Samadi, Alireza Yousefi

{"title":"Protective effects of wheat sprout extract on acrylamide-induced toxicity in testis, prostate gland and sperm parameters of rats","authors":"Hamid Reza Moradi, Nasrin Kazemipour, Saeed Nazifi, Melika Khodayari, Laleh Samadi, Alireza Yousefi","doi":"10.1007/s10735-025-10414-6","DOIUrl":"10.1007/s10735-025-10414-6","url":null,"abstract":"<div><p>Acrylamide (ACR) is a harmful compound that forms in food cooked at high temperatures, influenced by food type, preparation methods, temperature, and cooking time. Recent studies have indicated that ACR adversely affects male reproductive system and sperm health, primarily through oxidative stress. Wheat sprout (WS) is a unique medicinal plant that contains a high number of antioxidants. The main objective of this study was to investigate the possible effects of WS treatment on histological, biochemical and immunohistochemical changes in the testis and prostate as well as on the sperm parameters of rats exposed to ACR. Twenty adult male rats were split into four groups. One group received 1 mL normal saline, another group received 50 mg/kg ACR, third group received 200 mg/kg WS and fourth group received a combination of ACR (50 mg/kg) and WS (200 mg/kg). After 21 days, the epididymis was immediately examined for assessment of sperm. The prostate and left testis were placed in 10% formalin for histomorphometric and immunohistochemical analyses. The right testes were removed to measure testosterone, malondialdehyde (MDA) and total antioxidant capacity (TAC) levels. ACR consumption significantly decreased sperm count, viability, motility, and DNA fragmentation, whereas WS intake significantly improved these sperm parameters (<i>p</i> < 0.05). Compared with ACR, WS enhanced spermatogenesis indices, including TDI- and SI-positive seminiferous tubules, Johnson score, testis weight, body weight, and relative weight (<i>p</i> < 0.05). Furthermore, WS significantly reduced p53 expression and increased Bcl-2 expression, thereby counteracting ACR-induced apoptosis. The findings suggest that WS may effectively enhance and restore the histomorphometric, cellular, and hormonal changes in the male reproductive system caused by ACR.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143849019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosomes from miR-21-5p-modified adipose-derived stem cells promote wound healing by regulating M2 macrophage polarization in a rodent model of pressure ulcer 在啮齿动物压疮模型中，来自mir -21-5p修饰的脂肪来源干细胞的外泌体通过调节M2巨噬细胞极化促进伤口愈合

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-04-18 DOI: 10.1007/s10735-025-10407-5

Yongsheng Su, Zhibin Huang, Yuanwen Chen, Jingcheng Deng, Yubin Huang, Wei Xiong

{"title":"Exosomes from miR-21-5p-modified adipose-derived stem cells promote wound healing by regulating M2 macrophage polarization in a rodent model of pressure ulcer","authors":"Yongsheng Su, Zhibin Huang, Yuanwen Chen, Jingcheng Deng, Yubin Huang, Wei Xiong","doi":"10.1007/s10735-025-10407-5","DOIUrl":"10.1007/s10735-025-10407-5","url":null,"abstract":"<div><p>Pressure ulcers represent a significant healthcare burden worldwide. Numerous research has demonstrated the therapeutic potential of adipose-derived stem cell (ADSC)-derived exosomes in promoting wound healing. This study aims to investigate whether exosomes derived from miRNA-modified ADSCs play a role in pressure ulcers by affecting inflammation and macrophage polarization. ADSCs were identified by detecting the surface markers and multilineage differentiation potential. Lentiviruses carrying miR-21-5p were transduced in ADSCs for stable overexpression. Exosomes were extracted from ADSCs and identified. RT-qPCR was employed to detect RNA levels. A mouse model of pressure ulcers was established, followed by injection of exosomes. DiO staining was conducted to assess exosome biodistribution at wound sites. Hematoxylin-eosin and Masson staining were conducted for histological analysis. Immunofluorescence staining was used to evaluate TNF-α and IL-6 expression in mouse wound tissues. Western blotting was conducted to evaluate protein levels of macrophage polarization markers in vivo and in vitro. The results revealed that exosomes derived from miR-21-5p-overexpressing ADSCs promoted wound healing and reduced inflammatory cytokine expression in mouse wound tissues. Moreover, exosomal miR-21-5p induced macrophage M2 polarization in both mouse wound tissues and bone marrow-derived macrophages. Mechanistically, exosomal miR-21-5p inhibited NF-κB signal transduction in mouse wound tissues. In conclusion, ADSC-derived exosomes promote M2 macrophage polarization and inhibit inflammatory response in pressure ulcers via miR-21-5p delivery.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of ozone exposure on apoptotic mechanisms in Colo-320 and Colo-741 cell lines: implications for cancer therapy 臭氧暴露对Colo-320和Colo-741细胞系凋亡机制的影响：对癌症治疗的影响

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-04-18 DOI: 10.1007/s10735-025-10409-3

Remziye Kendirci-Katirci, Ertan Katirci, Tuna Onal, H. Seda Vatansever

{"title":"The effects of ozone exposure on apoptotic mechanisms in Colo-320 and Colo-741 cell lines: implications for cancer therapy","authors":"Remziye Kendirci-Katirci, Ertan Katirci, Tuna Onal, H. Seda Vatansever","doi":"10.1007/s10735-025-10409-3","DOIUrl":"10.1007/s10735-025-10409-3","url":null,"abstract":"<div><p>This study aimed to investigate the effects of ozone exposure on apoptotic mechanisms in primary (Colo-320) and metastatic (Colo-741) cell lines. Colo-320 and Colo-741 cells were grown in RPMI-1640 medium supplemented with 10% fetal bovine serum and treated with 20 µg/mL ozone for 72 h after MTT assay. Inverted microscopy was used for morphological assessment, and immunocytochemistry followed by H-SCORE analysis was performed to measure the expression of apoptotic markers. The statistical analysis was conducted using unpaired T-tests or Mann–Whitney U tests, with significance set at <i>p</i> < 0.05. Morphological analysis showed no significant changes in cell shape in Colo-320 or Colo-741 cells after ozone exposure. However, immunocytochemical analysis revealed significant increases in semi-quantitative histological scores (H-SCORES) for cleaved caspase-3, Bax, and cytochrome c in both cell lines, indicating enhanced apoptotic activity (<i>p</i> < 0.05). Conversely, Bcl-2 expression was significantly decreased in both Colo-320 and Colo-741 cell lines after ozone exposure (<i>p</i> < 0.05). Ozone exposure promoted apoptosis in both Colo-320 and Colo-741 cell lines, as evidenced by increased pro-apoptotic markers and decreased anti-apoptotic markers. These molecular changes were notable, yet they did not visibly alter cell morphology. The observed similarities between Colo-320 and Colo-741 cell responses suggest the need for further investigation. These findings indicate that ozone exposure may influence tumor cell apoptosis while preserving cell structure, highlighting the necessity of further research into its potential therapeutic implications.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0