Myogenic induced adipose-derived stem cells sheets combined with electrospun scaffolds of silk fibroin and poly(lactide-co-glycolide) for stress urinary incontinence treatment

IF 2.2 4区生物学 Q3 CELL BIOLOGY

Journal of Molecular Histology Pub Date : 2025-09-10 DOI:10.1007/s10735-025-10531-2

Ming Liu, Yongwei Li, Yining Zhao, Fengze Sun, Youyi Lu, Jitao Wu, Qingsong Zou

{"title":"Myogenic induced adipose-derived stem cells sheets combined with electrospun scaffolds of silk fibroin and poly(lactide-co-glycolide) for stress urinary incontinence treatment","authors":"Ming Liu, Yongwei Li, Yining Zhao, Fengze Sun, Youyi Lu, Jitao Wu, Qingsong Zou","doi":"10.1007/s10735-025-10531-2","DOIUrl":null,"url":null,"abstract":"<div><p>The stress urinary incontinence (SUI) is a difficulty in urology and current sub-urethral sling treatments are associated with inflamation and recurrence. In this study, we developed a novel tissue-engineered sling with myogenic induced adiposederived stem cells (MI-ADSCs) sheets induced by 5-Aza and combined with electrospun scaffolds of silk fibroin and poly(lactide-co-glycolide) (SF/PLGA) for the treatment of stress urinary incontinence. MI-ADSCs increased α-SMA, MyoD and Desmin the mRNA and protein expression. ICG001, a specific inhibitor of β-catenin signaling pathway, could inhibit 5-Aza-induced expression of myoblast genes and proteins. Electrospun scaffolds of SF/PLGA (50/50) showed favorable mechanical properties and the biocompatibility. The MI-ADSCs sheets was combined with electrospun scaffolds of SF/PLGA (50/50) to form a novel tissue-engineered sling. The tissue-engineered sling was transplanted into female SUI rats to evaluate the feasibility and effcacy for the treatment of SUI. The tissue-engineered sling with MI-ADSCs sheets combined with SF/PLGA can restore leak point pressure (LPP) in the model of SUI steadily. It is of great potential to be a novel tissue-engineered sling in the treatment of SUI.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 5","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Molecular Histology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10735-025-10531-2","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The stress urinary incontinence (SUI) is a difficulty in urology and current sub-urethral sling treatments are associated with inflamation and recurrence. In this study, we developed a novel tissue-engineered sling with myogenic induced adiposederived stem cells (MI-ADSCs) sheets induced by 5-Aza and combined with electrospun scaffolds of silk fibroin and poly(lactide-co-glycolide) (SF/PLGA) for the treatment of stress urinary incontinence. MI-ADSCs increased α-SMA, MyoD and Desmin the mRNA and protein expression. ICG001, a specific inhibitor of β-catenin signaling pathway, could inhibit 5-Aza-induced expression of myoblast genes and proteins. Electrospun scaffolds of SF/PLGA (50/50) showed favorable mechanical properties and the biocompatibility. The MI-ADSCs sheets was combined with electrospun scaffolds of SF/PLGA (50/50) to form a novel tissue-engineered sling. The tissue-engineered sling was transplanted into female SUI rats to evaluate the feasibility and effcacy for the treatment of SUI. The tissue-engineered sling with MI-ADSCs sheets combined with SF/PLGA can restore leak point pressure (LPP) in the model of SUI steadily. It is of great potential to be a novel tissue-engineered sling in the treatment of SUI.

查看原文本刊更多论文

肌源性脂肪干细胞片联合丝素-聚乳酸-羟基乙酸酯电纺丝支架治疗应激性尿失禁

压力性尿失禁（stress urinary incontinence， SUI）是泌尿外科的一个难点，目前的尿道下吊带治疗与炎症和复发有关。在这项研究中，我们开发了一种新型的组织工程吊带，该吊带由5-Aza诱导的肌源性脂肪源性干细胞（MI-ADSCs）片与丝素蛋白和聚乳酸-羟基乙酸酯（SF/PLGA）电纺丝支架联合使用，用于治疗应激性尿失禁。MI-ADSCs增加α-SMA、MyoD和Desmin mRNA和蛋白的表达。ICG001是β-catenin信号通路特异性抑制剂，可抑制5- aza诱导的成肌细胞基因和蛋白的表达。SF/PLGA（50/50）电纺丝支架具有良好的力学性能和生物相容性。将MI-ADSCs片与SF/PLGA（50/50）的电纺丝支架结合，形成一种新型的组织工程吊索。将组织工程吊带移植雌性SUI大鼠，评价其治疗SUI的可行性和效果。MI-ADSCs片与SF/PLGA结合的组织工程吊索可以稳定恢复SUI模型的泄漏点压力（LPP）。作为一种新型的组织工程吊索在SUI的治疗中具有很大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Molecular Histology 生物-细胞生物学

CiteScore

5.90

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.