2012 International Conference on Biomedical Engineering and Biotechnology最新文献_第4页

Synthesis and Evaluation of a Folate-linked Anti-cancer Prodrug 一种叶酸联抗肿瘤前药的合成与评价

2012 International Conference on Biomedical Engineering and Biotechnology Pub Date : 2012-05-28 DOI: 10.1109/ICBEB.2012.388

Duan Changli, Tian Jun-mei, Wang Shunan, Cao Jie, Chi Xuemei, Guan Yueqing

{"title":"Synthesis and Evaluation of a Folate-linked Anti-cancer Prodrug","authors":"Duan Changli, Tian Jun-mei, Wang Shunan, Cao Jie, Chi Xuemei, Guan Yueqing","doi":"10.1109/ICBEB.2012.388","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.388","url":null,"abstract":"Doxorubicin (DOX) is a highly active anti-cancer drug for the clinical use, but its therapeutic effectiveness is limited by its severe cardio toxic side effects and dose limiting. In order to over come these limitations, we produced a folate-bovine serum albumin(BSA)-cis-aconitic anhydride-doxorubicin (FA-BSA-CAD) conjugates. Folic acid (FA) is an essential vitamin and has high affinity to folate receptor (FR). FA was bound to BSA for increasing the active targeting of the conjugates. BSA was used as a carrier to provide passive tumor targeting by the enhanced permeability and retention (EPR) effect. DOX is conjugated with BSA with a pH sensitive linker cis-aconitic anhydride (CAA), which can be hydrolyzed in acidic lyzosomal environment. The conjugate was purified and separated by a Sephadex G-25 column. The successful synthesized of FA-BSA-CAD conjugates was further confirmed by mass spectrometry, UV-visible spectroscopy and FTIR. The therapeutical efficacy of the product was evaluated on three different tumor lines (BEL-7402, MDA-MB-231, MCF-7), with normal cell line as control (HELF). Results indicated that FA-BSA-CAD has selectively targeting ability to tumor cell lines, with less toxicity to the normal cells. In addition, the therapeutical efficacy is obvious increased compared to the pure DOX.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89098057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Impedimetric PSA Immunosensor: Prostate Specific Antigen Biosensor 阻抗性PSA免疫传感器:前列腺特异性抗原生物传感器

2012 International Conference on Biomedical Engineering and Biotechnology Pub Date : 2012-05-28 DOI: 10.1109/ICBEB.2012.247

C. Ching, Tzong-Ru Chou, T. Sun, H. Shieh

引用次数: 1

Enantioseparation of Enantiomers across Hollow Fiber Supported Liquid Membranes 中空纤维支撑液膜对映体的分离

2012 International Conference on Biomedical Engineering and Biotechnology Pub Date : 2012-05-28 DOI: 10.1109/ICBEB.2012.195

D. Huang, Wei Liu, Zhaolong Huang, Yashun Chen, Jing Wang, Na Wu

引用次数: 0

Removal of Non-uniform Stripe Noises from AFM Images AFM图像中非均匀条纹噪声的去除

2012 International Conference on Biomedical Engineering and Biotechnology Pub Date : 2012-05-28 DOI: 10.1109/ICBEB.2012.335

J. Pellequer, Y. Chen, S. W. Chen

引用次数: 4

Baculovirus-Mediated Gene Delivery for Bone Engineering 杆状病毒介导的骨工程基因传递

2012 International Conference on Biomedical Engineering and Biotechnology Pub Date : 2012-05-28 DOI: 10.1109/ICBEB.2012.91

Yu-Chen Hu, Chin-Yu Lin

{"title":"Baculovirus-Mediated Gene Delivery for Bone Engineering","authors":"Yu-Chen Hu, Chin-Yu Lin","doi":"10.1109/ICBEB.2012.91","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.91","url":null,"abstract":"Baculovirus has emerged to be a promising gene delivery vector and adipose-derived stem cells have captured interests for bone regeneration. Massive segmental defects arising from trauma or tumor resection remain a challenging clinical problem. To heal massive, segmental bone defects using adipose derived stem cells (ASCs), which alone cannot heal large defects, we hypothesized that sustained expression of factors promoting bone regeneration (BMP2) and angiogenesis (VEGF) provides continuous stimuli to augment the healing. Insect baculovirus (BV) holds promise for gene therapy and efficiently transduces stem cells, but it only mediates transient transgene expression. Therefore we developed a dual BV system whereby one BV expressed FLP recombinase (BacFLP) while the other hybrid BV harbored an Frt-flanking transgene cassette. After sequential transduction with BacFLP and the hybrid BV, FLP/Frt-mediated recombination occurred in up to 46% of ASCs, leading to cassette excision off the BV genome, formation and persistence of episomal transgene and prolongation of expression to >;28 days. Transduction of ASCs with the BMP2-encoding hybrid BV led to prolonged BMP2 expression and augmented ASCs osteogenesis even without other osteogenic supplements. ASCs engineered by the hybrid vectors mediating sustained BMP2/VEGF expression healed the critical-size (10 mm) segmental bone defects in 12 out of 12 New Zealand White rabbits in 8 weeks, which significantly outperformed ASCs engineered with BV transiently expressing BMP2/VEGF with respect to healing rate, bone metabolism, bone volume, bone density, angiogenesis and mechanical properties. These data confirmed our hypothesis that persistent BMP2/VEGF expression is essential. The use of the hybrid BV vector represents a novel therapy to treat massive segmental defects necessitating concerted ossification and vascularization.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87350232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Different Mixed Fertilizers on the Growth and Development in Stevia Rebaudiana Bertoni 不同混合肥料对甜菊叶生长发育的影响

2012 International Conference on Biomedical Engineering and Biotechnology Pub Date : 2012-05-28 DOI: 10.1109/ICBEB.2012.478

Liyuan Yan, X. Liu, Yan Shi

引用次数: 1

Stem Tissue Culture of Pinus elliottii × Pinus caribaea 湿地松与东北松的茎组织培养

2012 International Conference on Biomedical Engineering and Biotechnology Pub Date : 2012-05-28 DOI: 10.1109/ICBEB.2012.366

C. Lv, Baoling Huang

引用次数: 1

Determination of Quinine with Phosphotungstic Acid Modified Ion Sensitive Field Effect Transistor Sensor 磷钨酸修饰离子敏感场效应晶体管传感器测定奎宁

2012 International Conference on Biomedical Engineering and Biotechnology Pub Date : 2012-05-28 DOI: 10.1109/ICBEB.2012.135

Xianwen Li, Hefeng Xu

引用次数: 0

3D Liver Segmentation and Model Reconstruction from CT Images 基于CT图像的三维肝脏分割与模型重建

2012 International Conference on Biomedical Engineering and Biotechnology Pub Date : 2012-05-28 DOI: 10.1109/ICBEB.2012.18

Ya-Yun Cheng, Hsiao-Mei Chang, Hong-Ren Su, S. Lai, Kai-Che Liu, Chien Lin

引用次数: 3

The Changes of Submaxilary Glands Trefoil Factor2 Gene and Trefoil Factor3 Gene during Experimental Gastric Ulcer in Rat 实验性胃溃疡大鼠上颌下腺三叶因子2和三叶因子3基因的变化

2012 International Conference on Biomedical Engineering and Biotechnology Pub Date : 2012-05-28 DOI: 10.1109/ICBEB.2012.401

Jing-Fang Wu, Jing Zhang

{"title":"The Changes of Submaxilary Glands Trefoil Factor2 Gene and Trefoil Factor3 Gene during Experimental Gastric Ulcer in Rat","authors":"Jing-Fang Wu, Jing Zhang","doi":"10.1109/ICBEB.2012.401","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.401","url":null,"abstract":"To explore the expression changes of trefoil factor 2 (TFF2) and trefoil factor3 (TFF3) gene in submandibular glands during the self healing of experimental gastric ulcer in rats for revealing the relationship between them. The expression of TFF2, TFF3 peptide and genes were detected by immunohistochemistry and RT-PCR methods respectively in gastric ulcer group ,saline control group and normal group. The results were as follows: (1) TFF2 peptide showed strong expression in dark cells with few granules or in those cells without granules, but weakly positive or negative in cells with many granules. The expression was stronger in male rats than that in female rats. The optical density values (OD) values obviously increased on the day 1 after gastric ulcer (P<;0.01), and the lowest on the day 2, then gradually increased again on the day 4, 6 (P<;0.01) , and kept on a high level till 10d to 23d (P<;0.05). Compared with normal group, the OD of TFF2/GAPDH in 1,4,6,10,14 and 23d in gastric ulcer group were obviously higher than that in normal group except the 2d (P<;0.01 or P<;0.05). (2) TFF3 expression was higher in gastric ulcer than that in normal groups on the first day after surgery (P<;0.01), the optical density values (OD) were lower on the 2nd day than that on the 1st day (P<;0.05), then increased gradually on the 4th day and 6th day after gastric ulcer, and reached the highest on the 10th day (P<;0.01), It was still higher on the 23rd than that in normal group (P<;0.05). The OD values of TFF3/GAPDH in gastric ulcer group on the day of 1, 2, 4, 6, 10, 14, 23 are obviously higher than that in normal group (P<;0.01). Rat submaxillary glands TFF2 and TFF3 gene is chiefly in submaxillary ducts of normal and gastric ulcer rat.They may participate in the regulation of self-healing in gastric ulcer with the maner of exocrine.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84853600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0