2012 International Conference on Biomedical Engineering and Biotechnology最新文献

{"title":"Photosynthtic Inhibition on the Microalga Dunaliella sallina (Chlorophyta) by the Dried Macroalga Gracilaria lemaneiformis (Rhodophyta)","authors":"C. Ye, M. C. Zhang, T. Ganapathy, Y. Zuo, Y. F. Yang","doi":"10.1109/ICBEB.2012.296","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.296","url":null,"abstract":"The effects of the dried macro alga, Gracilaria lemaneiformis (Rhodophyta), on photosynthesis of a highly stress-resistant micro alga, Dunaliella sallina, were studied. D. sallina was cultured with dried G. lemaneiformis under controlled laboratory conditions. Photosynthetic oxygen evolution was measured and the chlorophyll a (Chl a) fluorescence transient O-J-I-P curve coupled with its specific parameters was established. In vivo, the Chl a fluorescence transients were recorded at high time resolution and analyzed via the JIP-test to evaluate photo system II activity. A clear dose-relevant relationship was estabished between the dried biomass of G. lemaneiformis and its inhibitory effect on D. sallina. A decrease in the light-saturated maximum photosynthetic oxygen evolution rate (Pmax) was observed, which was also time-relevant. Also an O-J-I-P curve along with its specific parameters were reduced. The dark respiration rate (Rd) showed a reverse pattern. The main photosynthetic inhibitory targets of the macro alga on the micro alga, based on the JIP-test and the amount of oxygen evolution, can be expressed as the number decrease of active reaction centers, the blocking of the electron transport chain, and the damage to the oxygen-evolving complex. The results of this study indicate that dried fragments of G. lemaneiformis effectively inhibit photosynthesis in D. sallina and could thus be potential candidates in controlling and mitigating harmful algal blooms.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"70 1","pages":"400-404"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73823178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction and Selection of Stenotrophomonas maltophilia DHHJ for Feather Degradation","authors":"Xiaoqian Wu, Jing Wang, Yuan Zhuang, Zhang-Jun Cao, Mei-hua Zhou","doi":"10.1109/ICBEB.2012.248","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.248","url":null,"abstract":"Stenotrophomonas maltophilia DHHJ, a new feather-degrading bacterium, was subjected to mutation by ultraviolet light for 90 s. The mutant L2 was selected due to its greater activity in comparison with the wild strain. After incubation at 40°C for 6 days, the feathers degraded by mutant L2 were 1.3-fold more than S. maltophilia DHHJ, and the keratinase was secreted. After cultivation for 6 days in 5 L fermenter with 50% air saturation, the rate of feather degradation was 92.3% and the activity of keratinase produced was 8.6 U/mL by mutant L2. The results proved that mutant L2 had high genetic stability and could be possibly used in the industrial processes.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"35 1","pages":"1521-1524"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75104023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Forensic Determination of Toxication of Strychnos Nuxvomica","authors":"Xi-ji Shu, Wei Liu, Shumei Zhu","doi":"10.1109/ICBEB.2012.226","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.226","url":null,"abstract":"Strychnos nux-vomica has been used as a poisonous raw material for the preparation of Chinese medicinal herb for thousands of years in China, it is required that the herb should be specially processed before being put into clinical use. Since its treatment dosage is very close to its toxic dosage, poisoning frequently take place due to misuse, brucine and its derivatives are the principal component responsible for its pharmacodynamic effect and toxicity, strychnine is main component responsible for its toxicity. Both strychnine and brucine are typical antagonists of the glycine receptors on postsynatic membrane. Its toxic effects are mainly to cause central excitation. Because of its strong toxicity, narrow safety range and cumulative effect, the use of the drug in the clinical practice tends to cause poisoning, and poisoning of the drug is associated with the large dosage, and/or the long time use. Its toxicity also varies with its origins of production or batches. The symptoms of strychnos nuxvomica poisoning vary. The autopsy findings mainly include pathological changes caused by acute disorder of blood circulation, characterized by the congestion and edema of organs or pinpoint bleeding of mucosa or serous membrane. Long-term toxicity test revealed no characteristic pathological changes of vital organs in animals. For the detection of poisonous substances, the stomach tissue or stomach contents are the best choices, and urine, intestinal contents are the next best materials. Liver, brain and spinal medulla also have high level of strychnos nuxvomica after poisioning. In the forensic identification of strychnos nuxvomica poisoning, it is important to eliminate the other death causes, than get sufficient information concerning the source of the herb (variety, batch, origin of the product), the physical condition (metabolic status) of the patients or victim, dosage, and take the symptoms, pathological changes into consideration. Because of its accumulation, the possibility of the drug being given multiple-times and in small-dose should be considered. The detection of strychnos nuxvomica and quantitative analysis is essential and most reliable in the identification of strychnos nuxvomica poisoning. Exceptionally, great care should be exercised to differentiate it from the intoxication of tetra mine, fumarin and armazide and other spasm-causing agents.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"20 1","pages":"1482-1485"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75519889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis on Water Consumption and Conservation of Petrochemical Industry in China","authors":"Yajun Zhang, Ping Xu, Jing Wang, Cuimin Feng, Tao Chen","doi":"10.1109/ICBEB.2012.71","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.71","url":null,"abstract":"Emphasizing the important role of saving replenish water in recycled cooling water system to improving the level of water use in petrochemical industry, then the detailed analysis is made on the water consumption links of petrochemical industry. According to the features of each water consumption link separately, water-saving measures are proposed correspondingly aimed at maximizing the water conservation and promoting the level of water-saving in petrochemical industry.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"33 1","pages":"1217-1220"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76967717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterizing the Spatiotemporal Pattern of Land Use and Cover Change in Oujiang River Basin, China","authors":"Chi Xu, Junyu Lu, Chao Hong, Junjie Li, Fenfei Chen, Maosong Liu","doi":"10.1109/ICBEB.2012.101","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.101","url":null,"abstract":"Land use and cover change (LUCC) produced enormous environmental impacts throughout the world. In this study, we quantified the spatiotemporal pattern of LUCC in the Oujiang River Basin, Eastern China, using remotely sensed data, landscape metrics and spatial analysis techniques. Results showed that residential growth and cropland reduction were the major forms of LUCC during 1992-2007. Despite of small coverage, the bare lands continuously increased in the riparian areas, which could produce significant impacts on the aquatic ecosystems. Results from the landscape metrics showed that pattern of residential growth could be characterized by the coalescence-diffusion process, and the croplands were increasingly fragmented. The hotspots of cropland reduction presented an aggregated pattern in adjacent to the residential areas. The hotspots of deforestation mostly occurred in the riparian areas and around the existed croplands in 1992-2000, while they were prone to exist in the fringe of the cities and towns in 2000-2007. Associated with urbanization and riparian destruction, environmental risks could largely increase in the basin. Our study has useful implications for regional management of the environments.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"152 1","pages":"1264-1267"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76974531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Development of Neural Ischemia Tolerance Model Induced by NGF Combined with OGD in PC12 Cells","authors":"C. Mei, Hui-Min Liu","doi":"10.1109/ICBEB.2012.414","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.414","url":null,"abstract":"Stroke is a major disease in humans. To better study this disease, a good ischemia model of nerve cells is needed. Nerve growth factor (NGF) can induce PC12 cells to become neurons. Oxygen glucose deprivation (OGD) leads to hypoxia and neuronal ischemia. In this study, we used NGF and OGD to stimulate PC12 cells and convert them into neurons in order to establish an ischemia model. After stimulation with NGF (100ng/ml for 6 d), PC12 cells show a neuron-like function as measured by physiology and biochemistry. After 6 d of NGF stimulation, we performed OGD treatment for 16 hours to establish an oxygen glucose deprivation model. The results showed that PC12 cells transformed into cells that looked like neurons and that MAP2 was up-regulated in NGF-treated PC12 cells. Cell apoptosis was found to be up-regulated after NGF stimulation and OGD (5% CO2 and 95% N2, 1mmol/l NaS2O4 in sugar-free DMEM for 16 h). A western blot analysis showed that OGD treatment increased the expression of HIF-1. The apoptosis rate after 16 hours of OGD was 19.44%. These results may help to show that NGF treatment can be combined with OGD to establish an in vitro model of acute ischemic brain damage.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"56 1","pages":"1105-1108"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76418904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on Extractive Distillation of Dimethyldichlorosilane and Methyltrichlorosilane","authors":"Jihai Duan, Z. Wei, Jian-Lin Li","doi":"10.1109/ICBEB.2012.340","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.340","url":null,"abstract":"This paper introduced extractive distillation to overcome the drawbacks of conventional distillation in separating dimethyldichlorosilane and methyltrichlorosilane. Based on the prediction of UNIFAC model, this paper adopted vapor-liquid equilibrium experiment for the entrainer selection. Aspen plus was employed to simulate the extractive distillation process to determine the overall energy consumption and separation effect. Simulation results showed that extractive distillation of dimethyldichlorosilane and methyltrichlorosilane was industrially feasible.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"8 1","pages":"492-495"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76547007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Studies on Chemical Constituents and Biological Activities of Typhonium giganteum Engl. Tuber Extracts","authors":"Yan-ling Song, Baina Li, Yan-qiu Meng","doi":"10.1109/ICBEB.2012.50","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.50","url":null,"abstract":"The dried tuber of Typhonium giganteum Engl. as a Chinese traditional medicine (Baifuzi) was recorded in Chinese Pharmacopoeia, which has the effects of \"dispelling wind-phlegm\" and apoplexy with gurgling in the throat etc. Recently, it has been shown that Typhonium giganteum Engl. tuber extracts possess a wide range of pharmacological properties, including effects on anti-tyrosinase, anti-inflammation, immunomodulation, apoptosis induction in cancer cells and so on. In this paper, we summarized the chemical constituents, biological activities and metabolism research advancement of Typhonium giganteum Engl. tuber extracts.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"67 1","pages":"30-33"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76715493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Texture Analysis and Volumetry of Hippocampus and Medial Temporal Lobe in Patients with Alzheimer's Disease","authors":"Hong Xia, Longzheng Tong, Xiaoxia Zhou, Jing Zhang, Zhen Zhou, Weifang Liu","doi":"10.1109/ICBEB.2012.395","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.395","url":null,"abstract":"This study investigated the texture features and volumes of bilateral hippocampus and medial temporal lobe (MTL) in magnetic resonance imaging (MRI) in patients with Alzheimer's disease (AD). Texture features were extracted using gray level co-occurrence matrix (GLCM) in 29 elderly AD patients, 19 age-matched non-demented controls (EC) and 18 young healthy controls (YC). The correlation between texture features and corresponding volumes was analyzed. Significant differences were found in both HF and MTL between AD and EC group in both volumes (p<;0.001) and texture features (p<;0.01). In addition, the texture features were markedly correlated with the corresponding volumes of both sites (p<;0.01 for HF, and p<;0.05 for MTL). While no significant differences were detected between EC and YC group on both texture features and volumes of HF, which indicated that ageing-associated regional changes such as atrophy were distinct from the AD-associated changes. The results suggest that texture features may be valuable to detect the underlying pathological changes in AD and like volumetry, texture analysis may serve as an aid for AD diagnosis.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"11 1","pages":"905-908"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78989730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Analysis of Fructus forsythiae by Near-infrared Spectroscopy","authors":"Xing Wang, Yan Bai, Yan-ling Zhang, Yanjiang Qiao","doi":"10.1109/ICBEB.2012.326","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.326","url":null,"abstract":"In this paper, Near-infrared spectroscopy (NIRS) was introduced into the field of Chinese herbal medicines and a rapid analytical method which can not only differentiate the two species of Fructus forsythiae, QingQiao (QQ) and LaoQiao (LQ), but also determine the contents of Phillyrin, Forsythoside A and Moisture in Fructus forsythia was established. The total content of volatile oil in Fructus forsythia was used as an important parameter for discrimination of QQ and LQ. Two lignans components, Phillyrin and Forsythoside A were analyzed successively by high-performance liquid chromatography (HPLC). Scattering effect and baseline shift in the NIR spectra were corrected by several pre-processing methods. By using discriminant analysis, a model which can be used to identify the species of Fructus forsythiae was established and samples were separated successfully into two different clusters corresponding to QQ and LQ. Finally, partial least squares (PLS) regression was used to build the correlation model. The results showed that the correlation coefficients of the calibration models are R = 0.959 for the Moisture, R = 0.957 for Phillyrin and R = 0.960 for Forsythoside A. The outcome showed that NIRS can provide a simple and accurate way in the quality control of Chinese herbal medicine (CHM).","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"46 1","pages":"473-476"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83168531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}