{"title":"嗜麦芽窄养单胞菌DHHJ降解羽毛的诱导与筛选","authors":"Xiaoqian Wu, Jing Wang, Yuan Zhuang, Zhang-Jun Cao, Mei-hua Zhou","doi":"10.1109/ICBEB.2012.248","DOIUrl":null,"url":null,"abstract":"Stenotrophomonas maltophilia DHHJ, a new feather-degrading bacterium, was subjected to mutation by ultraviolet light for 90 s. The mutant L2 was selected due to its greater activity in comparison with the wild strain. After incubation at 40°C for 6 days, the feathers degraded by mutant L2 were 1.3-fold more than S. maltophilia DHHJ, and the keratinase was secreted. After cultivation for 6 days in 5 L fermenter with 50% air saturation, the rate of feather degradation was 92.3% and the activity of keratinase produced was 8.6 U/mL by mutant L2. The results proved that mutant L2 had high genetic stability and could be possibly used in the industrial processes.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"35 1","pages":"1521-1524"},"PeriodicalIF":0.0000,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Induction and Selection of Stenotrophomonas maltophilia DHHJ for Feather Degradation\",\"authors\":\"Xiaoqian Wu, Jing Wang, Yuan Zhuang, Zhang-Jun Cao, Mei-hua Zhou\",\"doi\":\"10.1109/ICBEB.2012.248\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Stenotrophomonas maltophilia DHHJ, a new feather-degrading bacterium, was subjected to mutation by ultraviolet light for 90 s. The mutant L2 was selected due to its greater activity in comparison with the wild strain. After incubation at 40°C for 6 days, the feathers degraded by mutant L2 were 1.3-fold more than S. maltophilia DHHJ, and the keratinase was secreted. After cultivation for 6 days in 5 L fermenter with 50% air saturation, the rate of feather degradation was 92.3% and the activity of keratinase produced was 8.6 U/mL by mutant L2. The results proved that mutant L2 had high genetic stability and could be possibly used in the industrial processes.\",\"PeriodicalId\":6374,\"journal\":{\"name\":\"2012 International Conference on Biomedical Engineering and Biotechnology\",\"volume\":\"35 1\",\"pages\":\"1521-1524\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-05-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2012 International Conference on Biomedical Engineering and Biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/ICBEB.2012.248\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2012 International Conference on Biomedical Engineering and Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/ICBEB.2012.248","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Induction and Selection of Stenotrophomonas maltophilia DHHJ for Feather Degradation
Stenotrophomonas maltophilia DHHJ, a new feather-degrading bacterium, was subjected to mutation by ultraviolet light for 90 s. The mutant L2 was selected due to its greater activity in comparison with the wild strain. After incubation at 40°C for 6 days, the feathers degraded by mutant L2 were 1.3-fold more than S. maltophilia DHHJ, and the keratinase was secreted. After cultivation for 6 days in 5 L fermenter with 50% air saturation, the rate of feather degradation was 92.3% and the activity of keratinase produced was 8.6 U/mL by mutant L2. The results proved that mutant L2 had high genetic stability and could be possibly used in the industrial processes.