细胞与分子免疫学杂志最新文献

{"title":"[Advances in the study of sialic acid-binding immunoglobulin-like lectins in ocular diseases].","authors":"Junwen Ouyang, Yun He, Kai Hu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In recent years, increased attention has been payed to the immune regulatory mechanisms underlying ocular diseases, which has gradually challenged the traditional perception of the eye as an \"immune-privileged\" organ. Sialic acid-binding immunoglobulin-like lectins (Siglecs), a family of glycan-recognizing receptors predominantly expressed on immune cells, are broadly involved in immune cell recognition, activation, and the regulation of inflammatory responses. This review provides a comprehensive overview of the expression profiles and functional roles of Siglecs in allergic, autoimmune, and degenerative ocular disorders, evaluating their potential as diagnostic biomarkers and therapeutic targets. These findings are expected to enhance our understanding of the immunopathology of ocular diseases and contribute to the development of novel strategies in precision intervention.</p>","PeriodicalId":61378,"journal":{"name":"细胞与分子免疫学杂志","volume":"42 8","pages":"723-728"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148809584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress on the correlation and treatment of the cGAS-STING pathway in systemic lupus erythematosus].","authors":"Runying Wang, Nan Jiang, Shuo Wang, Haiyan Han","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Systemic lupus erythematosus (SLE) is a chronic autoimmune disease, characterized by inflammation and immune-mediated damage to multiple organ systems. Emerging evidence demonstrates that the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signaling pathway plays a pivotal role in the pathogenesis of SLE. The cGAS-STING pathway is a pattern recognition pathway of the innate immune system. After cGAS senses double-stranded DNA or endogenous DNA in the cytoplasm, it generates 2'3'-cyclic GMP-AMP to mediate intracellular signal transduction. This process activates STING, which in turn promotes the production of downstream interferons and other immune factors, ultimately triggering infection responses, cellular stress, and tissue damage. Under physiological conditions, the cGAS-STING pathway exerts protective functions in host defense. However, factors such as persistent activation may lead to dysregulation of the pathway, resulting in autoimmune diseases. Recent studies have explored interventions targeting this pathway at multiple levels, including genes, mitochondrial membranes, exonucleases, and lipopolysaccharides(LPS), which result in varying degrees of inhibition. These approaches offer novel therapeutic strategies for SLE. This study comprehensively reviews the recent research progress on inhibiting SLE-related inflammatory factor production via the cGAS-STING signaling axis by dividing the cGAS-STING pathway into two stages: dsDNA sensing and intracellular signal transduction. It also summarizes the existing therapeutic approaches related to this pathway, providing insights for more effective treatment of SLE.</p>","PeriodicalId":61378,"journal":{"name":"细胞与分子免疫学杂志","volume":"42 8","pages":"755-760"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148809650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[PTPRO inhibitor alleviates cytoskeletal rearrangement and pyroptosis in brain microvascular endothelial cells in sepsis-associated encephalopathy via PI3K/AKT-related signaling].","authors":"Fengjiao Wu, Haitao Tang, Qianqian Xiong, Chang Li, Yuwei Song, He Cao, Mengjie Tang, Zhengyan Pan, Guiping Liu, Tianzhen Li, Zongming Gou, Wusheng Zhou, Zhongqing Qian, Huaxue Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Objective Sepsis-associated encephalopathy (SAE) is a common and severe complication of sepsis and is closely associated with blood-brain barrier (BBB) dysfunction and brain microvascular endothelial cell injury. Protein tyrosine phosphatase receptor type O (PTPRO) plays an important role in inflammatory signal transduction and cellular functional regulation; however, its role in regulating brain endothelial barrier function during SAE remains unclear. This study aimed to investigate the effects of a PTPRO inhibitor on cytoskeletal remodeling and pyroptosis-related molecular events in brain microvascular endothelial cells and to explore the underlying molecular mechanism. Methods A mouse SAE model was established by intraperitoneal injection of lipopolysaccharide (LPS). Brain inflammatory cytokines were measured, and BBB permeability was evaluated by detecting albumin leakage and Evans Blue extravasation. In vitro, mouse brain microvascular endothelial bEnd.3 cells were stimulated with LPS. Immunofluorescence, transendothelial electrical resistance (TEER), and Western blotting were used to detect PTPRO expression, filamentous actin (F-actin) remodeling, monolayer barrier function, caspase-11/gasdermin D (GSDMD)-related pyroptotic molecular events, and phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) phosphorylation. PTPRO siRNA was used to verify the pharmacological inhibition results. Results LPS significantly increased interleukin-1β and interleukin-6 levels in mouse brain tissues and enhanced albumin leakage and Evans Blue extravasation. Pretreatment with the PTPRO inhibitor attenuated LPS-induced BBB permeability damage. In vitro, LPS markedly upregulated PTPRO expression in bEnd.3 cells, reduced TEER, induced F-actin remodeling, cell contraction, and intercellular gap formation, and increased caspase-11 and GSDMD-N levels. PTPRO inhibition or PTPRO siRNA intervention reversed these changes to varying degrees and was accompanied by reduced phosphorylation of PI3K and AKT. ConclusionPTPRO inhibition alleviates LPS-induced cytoskeletal remodeling, barrier dysfunction, and caspase-11/GSDMD-related pyroptotic molecular events in brain microvascular endothelial cells, possibly through PI3K/AKT-related signaling changes.</p>","PeriodicalId":61378,"journal":{"name":"细胞与分子免疫学杂志","volume":"42 8","pages":"692-700"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148809674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Non-invasive prenatal testing: The simulation application of QIAxcel Advanced automated nucleic acid analysis system in fetal Diego blood group detection].","authors":"Yongqiang Li, Yufeng Wang, Jiahui Huang, Qiurong Liu, Yuanming Wu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Objective To establish an QIAxcel Advanced automatic nucleic acid analysis system with high sensitivity and specificity for detecting the fetal Diego blood group. Methods Preliminary simulation of non-invasive prenatal detection of different proportions of fetal free DNA content in maternal plasma. QIAxcel Advanced automatic nucleic acid analysis system was used to detect the Di^a antigen sequences in simulated DNA to verify the accuracy and sensitivity of the method. ResultsQIAxcel Advanced automatic nucleic acid analysis system successfully detected the Di^a antigen sequences in different proportions of simulated DNA. Moreover, the sex-determining region Y (SRY) gene sequences of male fetuses in different proportions of simulated DNA were detected. Conclusion The QIAxcel Advanced automatic nucleic acid analysis system can be used for non-invasive prenatal detection of the DI*A genotype in Diego blood group system.</p>","PeriodicalId":61378,"journal":{"name":"细胞与分子免疫学杂志","volume":"42 8","pages":"708-714"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148809583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[To explore the immune landscape of lung adenocarcinoma patients with draining lymph nodes based on single cell sequencing].","authors":"Tingwei Shi, Wen Li, Zhiliang Fan, Hanshen Liu, Qing Zhao, Yilong Yu, Xiang Pu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Objective To explore the immune landscape of patients with lung adenocarcinoma with drainage lymph nodes by single-cell sequencing technology. Methods The lung cancer single cell dataset (GSE277742) was obtained from the Gene Expression Omnibus (GEO) database of the National Center for Biotechnology Information (NCBI). The dataset included intrathoracic DLN from 18 patients with pathologically confirmed lymph node metastasis and 4 control patients without evidence of metastasis. R language was used for data quality control, dimensionality reduction clustering, cell subgroup annotation and cell communication analysis to identify key cell subsets and screen differentially expressed genes. Kaplan-Meier survival analysis was performed using the TCGA database. Results Single cell sequencing analysis showed that the proportion of CD8⁺ T cells in patients with draining lymph nodes was significantly higher, among which cyclin-dependent kinase 6(CDK6), cyclin D3(CCND3), CCNH, cyclin-dependent kinase inhibitor 2A/B/D(CDKN2A/B/D) and retinoblastoma transcriptional corepressor 1(RB1) were highly expressed. TCGA database analysis showed that the expression of genes related to the BIOCARTA_CELLCYCLE_PATHWAY pathway was significantly different between tumor and normal samples, and was related to survival. Conclusion Several key genes differentially expressed in the draining lymph nodes of patients with lung adenocarcinoma are screened by bioinformatics methods, which provides a new perspective for further analysis of the regulatory mechanism of the immune microenvironment of lung adenocarcinoma with draining lymph nodes.</p>","PeriodicalId":61378,"journal":{"name":"细胞与分子免疫学杂志","volume":"42 7","pages":"616-624"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148474431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The dual effects of letermovir in allogeneic hematopoietic stem cell transplantation].","authors":"Jiaying Gao, Yueru Ji, Xiaotong Gao, Weiwei Qin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Human cytomegalovirus (HCMV) infection is one of the most common and severe viral complications following allogeneic hematopoietic stem cell transplantation (allo-HSCT), significantly increasing the risk of graft-versus-host disease (GVHD) and non-relapse mortality. Letermovir, as the first inhibitor targeting the HCMV terminase complex, blocks the cleavage of viral DNA and its packaging into viral capsids, demonstrating remarkable efficacy in early post-transplant HCMV prophylaxis. It significantly reduces the incidence of clinically significant HCMV infection (csCMVi) and indirectly contributes to improved GVHD- and relapse-free survival. However, while the potent viral suppression of this treatment provides clinical benefits, it also limits viral antigen exposure, which leads to delays in the reconstitution of HCMV-specific T cells and NK cells and consquently the increased risk of late-onset HCMV reactivation after drug discontinuation. This may also be associated with an elevated risk of Epstein-Barr virus reactivation and post-transplant lymphoproliferative disease (PTLD). This review aims to systematically summarize the dual effects of letermovir in allo-HSCT and analyzes its pharmacological mechanisms, clinical efficacy, impact on immune reconstitution, and potential consequences. Furthermore, it explores individualized management strategies based on immune monitoring to provide a theoretical basis for optimizing clinical practice.</p>","PeriodicalId":61378,"journal":{"name":"细胞与分子免疫学杂志","volume":"42 7","pages":"632-638"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148474346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The mechanism and research progress of thymic stromal lymphopoietin in chronic wound fibrosis].","authors":"Huafa Que, Yanchun Wang, Junqing Pan, Zhangren Yan","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The formation of chronic refractory wound (CRW) is the result of the combined effect of multiple factors, among which fibrosis plays a dual role in the pathogenesis. Moderate fibrosis can protect the wound, whereas excessive fibrosis leads to pathological scarring, which aggravates tissue hypoxia and stiffness, thereby impeding healing. Thymic stromal lymphopoietin (TSLP) is an important immunoregulatory cytokine that plays a critical role in allergic diseases. Recent studies have revealed that TSLP is also involved in the fibrotic mechanisms underlying CRWs. TSLP regulates key signaling pathways, including transforming growth factor-beta/Sma- and Mad-related protein (TGF-β/SMAD), wingless-type mice mammary tumour virus integration site family/beta-catenin (Wnt/β-catenin), and phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT), thereby affecting fibroblast activation, macrophage polarization, chemokine expression, and lysyl oxidase-mediated collagen cross-linking, ultimately contributing to CRW fibrosis. Future studies should focus on exploring the synergistic effects of TSLP with other profibrotic factors and developing combination therapies to balance fibrosis and tissue repair, thereby providing new strategies for the treatment of CRW fibrosis.</p>","PeriodicalId":61378,"journal":{"name":"细胞与分子免疫学杂志","volume":"42 7","pages":"660-666"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148474354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Isolation, expansion, and identification of the perivascular adipose-derived mesenchymal stem cells from rat aortas].","authors":"Huagen Ma, Ming Chi, Han Zhao, Yu Miao, Rong Yuan, Qiqi Xin, Juan Chen, Zhitao Wang, Weili Shi, Yuanyu Tang, Weihong Cong","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Objective To establish a method for isolating and expanding perivascular adipose-derived mesenchymal stem cells (PV-ADSC) from rat aortas and to provide a reliable cellular model for investigating vascular remodeling and the related cardiovascular diseases. Methods Perivascular adipose tissue (PVAT) was aseptically collected from the aortas of 1-month-old Sprague Dawley (SD) rats, then rinsed, minced, digested, filtered, and seeded for culture. Upon reaching 80%-90% confluence, the cells were passaged for expansion, and their morphology and proliferation were continuously monitored. Passage 5 (P5) cells were harvested for flow cytometric analysis of surface CD marker expression, and directed differentiation assays with lineage-specific staining were performed to assess the trilineage (adipogenic, osteogenic, chondrogenic) differentiation potential. Results On day 4 of the primary culture, spindle-shaped, stellate, and polygonal cells were observed migrating from the explant edges. Between day 5 and 6, island-like cell clones emerged, and by day 7 to 8, the colonies had coalesced into a confluent monolayer with swirling or parallel alignment. After being passaged, the cells exhibited a homogeneous fibroblast-like spindle morphology and maintained robust proliferation, with no evident senescence or aberrant differentiation through P8. P5 cells showed high expression of the mesenchymal stem cells (MSC) surface markers CD90 (93.60±0.62)%, CD73 (86.80±2.40)%, CD44 (97.77±0.58)%, and CD29 (98.60±0.30)%, yielding a mean positivity rate of (94.19±5.00)%. In contrast, expressions of the hematopoietic stem cells (HSC) surface markers CD45 (5.42±0.78)%, CD34 (7.15±0.49)%, and CD11b/c (3.74±0.08)% were significantly lower, with a mean rate of only (5.44±1.55)%. Directed differentiation assays confirmed the cells' ability to differentiate into adipocytes, osteoblasts, and chondrocytes, as demonstrated by positive staining with Oil Red O, Alizarin Red, and Alcian Blue, respectively. Conclusion Using the method established in this study, rat aortic PV-ADSC were successfully isolated and expanded. These cells exhibited the morphological and surface marker profiles typical of MSC, possessed robust self-renewal capacity, and retained the potential for trilineage differentiation into adipogenic, osteogenic, and chondrogenic lineages. Collectively, these characteristics establish a reliable cellular model for studying vascular remodeling and the related cardiovascular diseases.</p>","PeriodicalId":61378,"journal":{"name":"细胞与分子免疫学杂志","volume":"42 7","pages":"607-615"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148474341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Iron metabolism dysregulation drives tumor progression: mechanisms and therapeutic strategies].","authors":"Zongyang Chen, Fei Wang, Xunru Zhang, Jiaqi Liang, Hongzhuan Li, Wei Xu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Malignant tumors are major diseases that threaten human health. A long-standing focus of research is how to eliminate tumor cells while minimizing damage to healthy tissues. This article systematically reviews the dual role of iron metabolism in tumorigenesis and progression. Firstly, disruption of iron homeostasis promotes malignant transformation through mechanisms including oxidative stress, signaling transduction, and epigenetic regulation. Secondly, ferroptosis triggered by iron overload offers a novel avenue for targeted cancer therapy. Based on the established relationship between iron metabolism and cancer progression, this paper proposes therapeutic strategies centered on iron chelators, ferroptosis inducers, and nanomaterials. It further elaborates on recent advances in combining iron metabolism modulation with chemotherapy, immunotherapy, epigenetic therapy, and other modalities. Finally, by elucidating the core mechanism through which iron metabolism promotes cancer, we suggest potential therapeutic directions, aiming to provide new insights for advancing precision oncology and developing novel anticancer agents.</p>","PeriodicalId":61378,"journal":{"name":"细胞与分子免疫学杂志","volume":"42 7","pages":"639-645"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148474374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A study on HNA-1~-5 genotypes and frequencies in the blood donor population of jiangsu province].","authors":"Xi Yu, Ling Ma, Lei Shao, Lili Shi, Taixiang Liu, Ruoyang Zhang, Fang Zhao, Hong Lin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Objective To investigate the distribution characteristics of the genotypes and allele frequencies of HNA-1~-5 alleles in a blood donor population from Jiangsu Province. Methods Genomic DNA was extracted from 300 peripheral blood samples collected from healthy donors. All samples were genotyped using an optimized polymerase chain reaction-sequence specific primer (PCR-SSP) protocol. Randomly selected results were verified by direct sequencing, and genotype distributions were tested for conformity to Hardy-Weinberg equilibrium. Samples identified as potential HNA-1 null were confirmed by polymerase chain reaction sequencing-based typing (PCR-SBT) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The allele frequencies for HNA-1a, HNA-1b, and HNA-1c were 58.6%, 41.4%, and 0%, respectively. Two identified HNA-1 null cases were confirmed to result from a complete deletion of the Fc gamma receptor IIIb (FCGR3B) gene. The allele frequency of the HNA-2-encoding CD177 gene was 100%, with no HNA-2 null cases detected due to the homozygous c.787 A>T (TT) mutation. The allele frequencies for HNA-3a, HNA-3b, HNA-4a, HNA-4b, HNA-5a, and HNA-5b were 63.0%, 37.0%, 100%, 0%, 88.8%, and 11.2%, respectively. No statistically significant differences in HNA allele frequencies were found between the Jiangsu population and those from Shanghai or Zhejiang. However, significant differences were observed when compared to the Guangzhou population specifically for the HNA-1a/1a (P=0.008), HNA-3a/3a (P=0.033), and HNA-3b/3b (P=0.022) genotypes. Conclusion This study establishes the HNA genotype and allele frequency profile for the Jiangsu blood donor population, providing essential baseline data for research into HNA-related alloimmunization. Furthermore, it optimizes a methodology suitable for large-scale HNA screening and establishes a comprehensive confirmatory testing protocol for HNA-1 null, thereby laying a technical foundation for subsequent research on this allele.</p>","PeriodicalId":61378,"journal":{"name":"细胞与分子免疫学杂志","volume":"42 7","pages":"625-631"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148474366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}