Lipi Pradhan, Prince Sah, Malay Nayak, Anjali Upadhyay, Pragya Pragya, Shikha Tripathi, Gurmeet Singh, B. Mounika, Pradip Paik, Sudip Mukherjee
{"title":"Biosynthesized silver nanoparticles prevent bacterial infection in chicken egg model and mitigate biofilm formation on medical catheters","authors":"Lipi Pradhan, Prince Sah, Malay Nayak, Anjali Upadhyay, Pragya Pragya, Shikha Tripathi, Gurmeet Singh, B. Mounika, Pradip Paik, Sudip Mukherjee","doi":"10.1007/s00775-024-02050-4","DOIUrl":"10.1007/s00775-024-02050-4","url":null,"abstract":"<div><p>Investigating the application of innovative antimicrobial surface coatings on medical devices is an important field of research. Many of these coatings have significant drawbacks, including biocompatibility, coating stability and the inability to effectively combat multiple drug-resistant bacteria. In this research, we developed an antibiofilm surface coating for medical catheters using biosynthesized silver nanoparticles (b-Cs-AgNPs) developed using leaves extract of <i>Calliandra surinamensis</i>. Various characterization techniques were employed to thoroughly characterize the synthesized b-Cs-AgNPs and c-AgNPs. b-Cs-AgNPs were compatible with human normal kidney cells and chicken embryos. It did not trigger any skin inflammatory response in in vivo rat model. b-Cs-AgNPs demonstrated potent zone of inhibition of 19.09 mm when subjected to the disc diffusion method in <i>E. coli</i> confirming strong antibacterial property. Different anti-bacterial assays including liquid growth curve, colony counting assay, biofilm formation assay supported the potent antimicrobial efficacy of b-Cs-AgNPs alone and when coated to medical grade catheters. Mechanistic studies reveal the presence of ferulic acid, that was important for the synthesis of b-AgNPs along with enhanced antibacterial effects of b-Cs-AgNPs compared to c-AgNPs, supported by molecular docking analysis. These results together demonstrated the effective role b-Cs-AgNPs in combating infections and mitigating biofilm formations, highlighting their need for further study in the field of biomedical applications.</p><h3>Graphical abstract</h3><p>Schematic Illustration of Eco-Friendly Synthesis for Biofilm Prevention on Medical Catheters and Bacterial Infection Mitigation. Created with BioRender.com.</p>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":603,"journal":{"name":"Journal of Biological Inorganic Chemistry","volume":"29 3","pages":"353 - 373"},"PeriodicalIF":2.7,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140920598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A commentary on studies of brain iron accumulation during ageing","authors":"Mark J. Hackett","doi":"10.1007/s00775-024-02060-2","DOIUrl":"10.1007/s00775-024-02060-2","url":null,"abstract":"<div><p>Brain iron content is widely reported to increase during “ageing”, across multiple species from nematodes, rodents (mice and rats) and humans. Given the redox-active properties of iron, there has been a large research focus on iron-mediated oxidative stress as a contributor to tissue damage during natural ageing, and also as a risk factor for neurodegenerative disease. Surprisingly, however, the majority of published studies have not investigated brain iron homeostasis during the biological time period of senescence, and thus knowledge of how brain homeostasis changes during this critical stage of life largely remains unknown. This commentary examines the literature published on the topic of brain iron homeostasis during ageing, providing a critique on limitations of currently used experimental designs. The commentary also aims to highlight that although much research attention has been given to iron accumulation or iron overload as a pathological feature of ageing, there is evidence to support functional iron deficiency may exist, and this should not be overlooked in studies of ageing or neurodegenerative disease.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":603,"journal":{"name":"Journal of Biological Inorganic Chemistry","volume":"29 4","pages":"385 - 394"},"PeriodicalIF":2.7,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11186910/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140907957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hiba Messaoudi, Göknur Yaşa Atmaca, Ayşegül Türkkol, Mehmet Dinçer Bilgin, Ali Erdoğmuş
{"title":"Monitoring of singlet oxygen generation of a novel Schiff-base substituted silicon phthalocyanines by sono-photochemical studies and in vitro activities on prostate cancer cell","authors":"Hiba Messaoudi, Göknur Yaşa Atmaca, Ayşegül Türkkol, Mehmet Dinçer Bilgin, Ali Erdoğmuş","doi":"10.1007/s00775-024-02055-z","DOIUrl":"10.1007/s00775-024-02055-z","url":null,"abstract":"<div><p>This study demonstrates the potential of sono-photodynamic therapy as an effective approach for enhancing singlet oxygen generation using the synthesized Schiff-base diaxially substituted silicon phthalocyanines. In photochemical studies, the singlet oxygen quantum yields (<b>Φ</b><sub><b>∆</b></sub>) were determined as 0.43 for <b>Si1a</b>, 0.94 for <b>Q-Si1a</b>, 0.58 for <b>S-Si1a</b>, and 0.49 for <b>B-Sia1</b>. In sono-photochemical studies, the Φ<sub>∆</sub> values were reached to 0.67 for <b>Si1a</b>, 1.06 for <b>Q-Si1a</b>, 0.65 for <b>S-Si1a</b>, and 0.67 for <b>B-Sia1</b>. In addition, this study demonstrates the therapeutic efficacy of phthalocyanines synthesized as sensitizers on the PC3 prostate cancer cell line through in vitro experiments. The application of these treatment modalities exhibited notable outcomes, leading to a substantial decrease in cell viability within the PC3 prostate cancer cell line. These findings highlight the potential of utilizing these synthesized phthalocyanines as promising therapeutic agents for prostate cancer treatment.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":603,"journal":{"name":"Journal of Biological Inorganic Chemistry","volume":"29 3","pages":"303 - 314"},"PeriodicalIF":2.7,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11111517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140896442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shanjia Li, Xupeng Zhang, Tiankun Zhao, Nan Liu, Yong Zhang, Peng Wang, Zhongduo Yang, Thomas Huhn
{"title":"Synthesis, in vitro antitumor evaluation and structure activity relationship of heptacoordinated amino-bis(Phenolato) Ti(IV) complexes stabilized by 2,6-dipicolinic acid","authors":"Shanjia Li, Xupeng Zhang, Tiankun Zhao, Nan Liu, Yong Zhang, Peng Wang, Zhongduo Yang, Thomas Huhn","doi":"10.1007/s00775-024-02059-9","DOIUrl":"10.1007/s00775-024-02059-9","url":null,"abstract":"<div><p>Eighteen novel Ti(IV) complexes stabilized by different chelating amino-<i>bis</i>(phenolato) (ONNO, ONON, ONOO) ligands and 2,6-dipicolinic acid as a second chelator were synthesized with isolated yields ranging from 79 to 93%. Complexes were characterized by <sup>1</sup>H and <sup>13</sup>C-NMR spectroscopy, as well as by HRMS and X-Ray diffraction analysis. The good to excellent aqueous stability of these Ti(IV) complexes can be modulated by the substitutions on the 2-position of the phenolato ligands. Most of the synthesized Ti(IV) complexes demonstrated potent inhibitory activity against Hela S3 and Hep G2 tumor cells. Among them, the naphthalenyl based Salan type <b>2j</b>, 2-picolylamine based [ONON] type <b>2n</b> and <i>N</i>-(2-hydroxyethyl) based [ONOO] type <b>2p</b> demonstrated up to 40 folds enhanced cytotoxicity compared to cisplatin together with a significantly reduced activity against healthy AML12 cells. The three Ti(IV) complexes exhibited fast cellular uptake by Hela S3 cells and induced almost exclusively apoptosis. <b>2j</b> could trigger higher level of ROS generation than <b>2p</b> and <b>2n</b>.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":603,"journal":{"name":"Journal of Biological Inorganic Chemistry","volume":"29 3","pages":"315 - 330"},"PeriodicalIF":2.7,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140896561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Levi A. Ekanger, Ruhi K. Shah, Matthew E. Porowski, Zach Ziolkowski, Alana Calello
{"title":"Spectroscopic, electrochemical, and kinetic trends in Fe(III)–thiolate disproportionation near physiologic pH","authors":"Levi A. Ekanger, Ruhi K. Shah, Matthew E. Porowski, Zach Ziolkowski, Alana Calello","doi":"10.1007/s00775-024-02051-3","DOIUrl":"10.1007/s00775-024-02051-3","url":null,"abstract":"<div><p>In addition to its primary oxygen-atom-transfer function, cysteamine dioxygenase (ADO) exhibits a relatively understudied anaerobic disproportionation reaction (ADO-Fe(III)-SR → ADO-Fe(II) + ½ RSSR) with its native substrates. Inspired by ADO disproportionation reactivity, we employ [Fe(tacn)Cl<sub>3</sub>] (tacn = 1,4,7-triazacyclononane) as a precursor for generating Fe(III)–thiolate model complexes in buffered aqueous media. A series of Fe(III)–thiolate model complexes are generated in situ using aqueous [Fe(tacn)Cl<sub>3</sub>] and thiol-containing ligands cysteamine, penicillamine, mercaptopropionate, cysteine, cysteine methyl ester, <i>N</i>-acetylcysteine, and <i>N</i>-acetylcysteine methyl ester. We observe trends in UV–Vis and electron paramagnetic resonance (EPR) spectra, disproportionation rate constants, and cathodic peak potentials as a function of thiol ligand. These trends will be useful in rationalizing substrate-dependent Fe(III)–thiolate disproportionation reactions in metalloenzymes.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":603,"journal":{"name":"Journal of Biological Inorganic Chemistry","volume":"29 3","pages":"291 - 301"},"PeriodicalIF":2.7,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11111527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140896449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anja Pomowski, Simone Dell’Acqua, Anja Wüst, Sofia R. Pauleta, Isabel Moura, Oliver Einsle
{"title":"Revisiting the metal sites of nitrous oxide reductase in a low-dose structure from Marinobacter nauticus","authors":"Anja Pomowski, Simone Dell’Acqua, Anja Wüst, Sofia R. Pauleta, Isabel Moura, Oliver Einsle","doi":"10.1007/s00775-024-02056-y","DOIUrl":"10.1007/s00775-024-02056-y","url":null,"abstract":"<div><p>Copper-containing nitrous oxide reductase catalyzes a 2-electron reduction of the green-house gas N<sub>2</sub>O to yield N<sub>2</sub>. It contains two metal centers, the binuclear electron transfer site Cu<sub>A</sub>, and the unique, tetranuclear Cu<sub>Z</sub> center that is the site of substrate binding. Different forms of the enzyme were described previously, representing variations in oxidation state and composition of the metal sites. Hypothesizing that many reported discrepancies in the structural data may be due to radiation damage during data collection, we determined the structure of anoxically isolated <i>Marinobacter nauticus</i> N<sub>2</sub>OR from diffraction data obtained with low-intensity X-rays from an in-house rotating anode generator and an image plate detector. The data set was of exceptional quality and yielded a structure at 1.5 Å resolution in a new crystal form. The Cu<sub>A</sub> site of the enzyme shows two distinct conformations with potential relevance for intramolecular electron transfer, and the Cu<sub>Z</sub> cluster is present in a [4Cu:2S] configuration. In addition, the structure contains three additional types of ions, and an analysis of anomalous scattering contributions confirms them to be Ca<sup>2+</sup>, K<sup>+</sup>, and Cl<sup>–</sup>. The uniformity of the present structure supports the hypothesis that many earlier analyses showed inhomogeneities due to radiation effects. Adding to the earlier description of the same enzyme with a [4Cu:S] Cu<sub>Z</sub> site, a mechanistic model is presented, with a structurally flexible Cu<sub>Z</sub> center that does not require the complete dissociation of a sulfide prior to N<sub>2</sub>O binding.</p><h3>Graphical Abstract</h3><p>The [4Cu:2S] CuZ site in M. nauticus N 2O reductase. The electron density map shown is contoured at the 5 \u0000σ level, highlighting the presence of two sulfide ligands. \u0000705x677mm (72 x 72 DPI)</p><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":603,"journal":{"name":"JBIC Journal of Biological Inorganic Chemistry","volume":"29 3","pages":"279 - 290"},"PeriodicalIF":2.7,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00775-024-02056-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140890824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amalia García-García, Pablo Cristobal-Cueto, Tania Hidalgo, Iñigo J. Vitórica-Yrezábal, Antonio Rodríguez-Diéguez, Patricia Horcajada, Sara Rojas
{"title":"Potential antiprostatic performance of novel lanthanide-complexes based on 5-nitropicolinic acid","authors":"Amalia García-García, Pablo Cristobal-Cueto, Tania Hidalgo, Iñigo J. Vitórica-Yrezábal, Antonio Rodríguez-Diéguez, Patricia Horcajada, Sara Rojas","doi":"10.1007/s00775-024-02054-0","DOIUrl":"10.1007/s00775-024-02054-0","url":null,"abstract":"<div><p>Two new lanthanide-complexes based on the 5-nitropicolinate ligand (5-npic) were obtained and fully characterized. Single-crystal X-ray diffraction revealed that these compounds are isostructural to a Dy-complex, previously published by us, based on dinuclear monomers link together with an extended hydrogen bond network, providing a final chemical formula of [Ln<sub>2</sub>(5-npic)<sub>6</sub>(H<sub>2</sub>O)<sub>4</sub>]·(H<sub>2</sub>O)<sub>2</sub>, where Ln = Dy <b>(1)</b>, Gd <b>(2)</b>, and Tb <b>(3)</b>. Preliminary photoluminescent studies exhibited a ligand-centered emission for all complexes. The potential antitumoral activity of these materials was assayed in a prostatic cancer cell line (PC-3; the 2nd most common male cancerous disease), showing a significant anticancer activity (50–60% at 500 μg·mL<sup>−1</sup>). In turn, a high biocompatibility by both, the complexes and their precursors in human immunological HL-60 cells, was evidenced. In view of the strongest toxic effect in the tumoral cell line provided by the free 5-npic ligand (~ 40–50%), the overall anticancer complex performance seems to be triggered by the presence of this molecule.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":603,"journal":{"name":"Journal of Biological Inorganic Chemistry","volume":"29 3","pages":"331 - 338"},"PeriodicalIF":2.7,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11111526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140875513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Garima Mann, Shivani Daksh, Nikhil Kumar, Ankur Kaul, B. G. Roy, M. Thirumal, Anupama Datta
{"title":"Pre-clinical evaluation of 99mTc-labeled chalcone derivative for amyloid-β imaging post-head trauma","authors":"Garima Mann, Shivani Daksh, Nikhil Kumar, Ankur Kaul, B. G. Roy, M. Thirumal, Anupama Datta","doi":"10.1007/s00775-024-02049-x","DOIUrl":"10.1007/s00775-024-02049-x","url":null,"abstract":"<div><p>Aβ<sub>42</sub> plaque formation is one of the preliminary pathologic events that occur post traumatic brain injury (TBI) which is also among the most noteworthy hallmarks of AD. Their pre symptomatic detection is therefore vital for better disease management. Chalcone–picolinic acid chelator derivative, 6‐({[(6‐carboxypyridin‐2‐yl)methyl](2‐{4‐[(2E)‐3‐[4‐(dimethyl amino)phenyl]prop‐2‐enoyl]phenoxy}ethyl)amino}methyl)pyridine‐2‐carboxylic acid, Py-chal was synthesized to selectively identify amyloid plaques formed post head trauma using SPECT imaging by stable complexation to <sup>99m</sup>Tc with > 97% efficiency without compromising amyloid specificity. The binding potential of the Py-chal ligand to amyloid plaques remained high as confirmed by in vitro binding assay and photophysical spectra. Further, the Py-chal complex stained amyloid aggregates in the brain sections of rmTBI mice model. In vivo scintigraphy in TBI mice model displayed high uptake followed by high retention while the healthy rabbits displayed higher brain uptake followed by a rapid washout attributed to absence of amyloid plaques. Higher uptake in brain of TBI model was also confirmed by ex vivo biodistribution analysis wherein brain uptake of 3.38 ± 0.2% ID/g at 2 min p.i. was observed for TBI mice model. This was followed by prolonged retention and more than twofold higher activity as compared to sham mice brain. This preliminary data suggests the specificity of the radiotracer for amyloid detection post head trauma and applicability of <sup>99m</sup>Tc labeled Py-chal complex for TBI-induced β-amyloid SPECT imaging.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":603,"journal":{"name":"JBIC Journal of Biological Inorganic Chemistry","volume":"29 2","pages":"187 - 199"},"PeriodicalIF":2.7,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140582140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mária Kováčová, Halyna Bodnár Yankovych, Adrian Augustyniak, Mariano Casas-Luna, Michaela Remešová, Lenka Findoráková, Martin Stahorský, Ladislav Čelko, Matej Baláž
{"title":"Triggering antibacterial activity of a common plant by biosorption of selected heavy metals","authors":"Mária Kováčová, Halyna Bodnár Yankovych, Adrian Augustyniak, Mariano Casas-Luna, Michaela Remešová, Lenka Findoráková, Martin Stahorský, Ladislav Čelko, Matej Baláž","doi":"10.1007/s00775-024-02045-1","DOIUrl":"10.1007/s00775-024-02045-1","url":null,"abstract":"<div><p>The presented study proposes an efficient utilization of a common <i>Thymus serpyllum</i> L. (wild thyme) plant as a highly potent biosorbent of Cu(II) and Pb(II) ions and the efficient interaction of the copper-laden plant with two opportunistic bacteria. Apart from biochars that are commonly used for adsorption, here we report the direct use of native plant, which is potentially interesting also for soil remediation. The highest adsorption capacity for Cu(II) and Pb(II) ions (<i>q</i><sub>e</sub> = 12.66 and 53.13 mg g<sup>−1</sup>, respectively) was achieved after 10 and 30 min of adsorption, respectively. Moreover, the Cu-laden plant was shown to be an efficient antibacterial agent against the bacteria <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>, the results being slightly better in the former case. Such an activity is enabled only via the interaction of the adsorbed ions effectively distributed within the biological matrix of the plant with bacterial cells. Thus, the sustainable resource can be used both for the treatment of wastewater and, after an effective embedment of metal ions, for the fight against microbes.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":603,"journal":{"name":"Journal of Biological Inorganic Chemistry","volume":"29 2","pages":"201 - 216"},"PeriodicalIF":2.7,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00775-024-02045-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140585589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Christina N. Banti, Foteini D. Kalousi, Anna-Maria G. Psarra, Eleni E. Moushi, Demetres D. Leonidas, Sotiris K. Hadjikakou
{"title":"Silver ciprofloxacin (CIPAG): a multitargeted metallodrug in the development of breast cancer therapy","authors":"Christina N. Banti, Foteini D. Kalousi, Anna-Maria G. Psarra, Eleni E. Moushi, Demetres D. Leonidas, Sotiris K. Hadjikakou","doi":"10.1007/s00775-024-02048-y","DOIUrl":"10.1007/s00775-024-02048-y","url":null,"abstract":"<div><p>The anti-proliferative activity of the known metalloantibiotic {[Ag(CIPH)<sub>2</sub>]NO<sub>3</sub>∙0.75MeOH∙1.2H<sub>2</sub>O} (<b>CIPAG</b>) (CIPH = ciprofloxacin) against the human breast adenocarcinoma cancer cells MCF-7 (hormone dependent (HD)) and MDA-MB-231 (hormone independent (HI)) is evaluated. The in vitro toxicity and genotoxicity of the metalloantibiotic were estimated toward fetal lung fibroblast (MRC-5) cells. The molecular mechanism of the <b>CIPAG</b> activity against MCF-7 cells was clarified by the (i) cell morphology, (ii) cell cycle arrest, (iii) mitochondrial membrane permeabilization, and (iv) by the assessment of the possible differential effect of <b>CIPAG</b> on estrogen receptor alpha (ERα) and estrogen receptor beta (ERβ) transcriptional activation, applying luciferase reporter gene assay. Moreover, the ex vivo mechanism of <b>CIPAG</b> was clarified by its binding affinity toward calf thymus (CT-DNA).</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":603,"journal":{"name":"Journal of Biological Inorganic Chemistry","volume":"29 2","pages":"177 - 186"},"PeriodicalIF":2.7,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00775-024-02048-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140602067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}