中国地球物理学会年刊最新文献_第10页

Cell tracking velocimetry for monocyte/endothelial cell interactions 单核细胞/内皮细胞相互作用的细胞跟踪测速仪

中国地球物理学会年刊 Pub Date : 2002-12-01 DOI: 10.1109/IEMBS.2002.1134526

P. Wong, C. H. Kwong, T. Hsiai, C. Ho

引用次数: 0

Optimization of PEI/VEGF DNA polyplexes for potenital delivery from tissue engineering scaffolds PEI/VEGF DNA复合物的优化，用于组织工程支架的潜在递送

中国地球物理学会年刊 Pub Date : 2002-12-01 DOI: 10.1109/IEMBS.2002.1136951

J. H. van Zanten, Y. Har-el, J. Hanes

{"title":"Optimization of PEI/VEGF DNA polyplexes for potenital delivery from tissue engineering scaffolds","authors":"J. H. van Zanten, Y. Har-el, J. Hanes","doi":"10.1109/IEMBS.2002.1136951","DOIUrl":"https://doi.org/10.1109/IEMBS.2002.1136951","url":null,"abstract":"Non-viral vector formation by DNA complexation with cationic condensing agents is a self-assembly process driven primarily by electrostatic interactions and counterion release. DNA complexation kinetics influence three physical parameters that have a direct effect on gene delivery and expression efficiency: DNA complex geometric size, surface charge and density. We demonstrate the utility of time resolved multiangle laser light scattering (TR-MALLS) for probing polyethylenimine (PEI) based polyplex formation kinetics with plasmid DNA. Our studies utilize plasmid DNA coding for VEGF, which may be used to enhance blood vessel in-growth into cell seeded polymeric scaffolds used in tissue engineering applications. We determined PEI/DNA complex size and density in real time and monitored vector stability in various liquid formulations. Parameters such as PEI molecular weight, N/P ratio and solution pH and ionic strength were investigated systematically. The ability to accurately measure polyplex size and density may lead to improvements in the design and control of non-viral gene delivery vectors and facilitate the determination of optimal formulations.","PeriodicalId":60385,"journal":{"name":"中国地球物理学会年刊","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2002-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85674599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elucidation of simple optic stimuli based on detected nerve activity 基于检测到的神经活动来解释简单的视觉刺激

中国地球物理学会年刊 Pub Date : 2002-12-01 DOI: 10.1109/IEMBS.2002.1134377

T.D. Coates, S. Wolpert

{"title":"Elucidation of simple optic stimuli based on detected nerve activity","authors":"T.D. Coates, S. Wolpert","doi":"10.1109/IEMBS.2002.1134377","DOIUrl":"https://doi.org/10.1109/IEMBS.2002.1134377","url":null,"abstract":"In this study, a minimally invasive interface to an intact peripheral nerve was developed. The interface was based on a cylindrical cuff installed around the nerve, and fitted with circumferentially dispersed surface electrodes. Differential measurements of bulk potentials were filtered, amplified, and subsequently processed with the objective of identifying unique patterns of electrical activity within the ensheathed nerve bundle. The system was implemented using an in vivo preparation of lateral eye and optic nerve from Limulus polyphemus. Recorded signals were filtered using the discrete wavelet transform and the Daubechies 4 wavelet basis to isolate signal components regarded as significant to the optical pattern. The entropies of the filtered signals were computed and used to build training and testing patterns for a cascade correlation neural network. The values on the eight outputs of the neural network represented the probability of one or more of the eight locations on the lateral eye having been illuminated by the stimulus. From these outputs, an image of the optical stimulus pattern was rendered. In tests, the system displayed an accuracy of 100% in distinguishing stimulus from nonstimulus conditions, and up to 91% in identifying the location(s) illuminated by a given pattern of optical stimulation.","PeriodicalId":60385,"journal":{"name":"中国地球物理学会年刊","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2002-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87224087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tissue engineering scaffolds for controlled protein and plasmid delivery 用于控制蛋白质和质粒递送的组织工程支架

中国地球物理学会年刊 Pub Date : 2002-12-01 DOI: 10.1109/IEMBS.2002.1136902

L. Shea, K. Whittlesey, T. Segura, J. Jang, Y. Yang

{"title":"Tissue engineering scaffolds for controlled protein and plasmid delivery","authors":"L. Shea, K. Whittlesey, T. Segura, J. Jang, Y. Yang","doi":"10.1109/IEMBS.2002.1136902","DOIUrl":"https://doi.org/10.1109/IEMBS.2002.1136902","url":null,"abstract":"Tissue engineering approaches employ polymer scaffolds to supports, promote, and direct tissue formation by progenitor cells, which are either present in the surrounding tissue or transplanted with the polymer. We hypothesize that scaffolds capable of delivering tissue-inductive factors, or genes encoding for these factors, at the correct time and location, and in the appropriate concentration could enhance the effectiveness of current regenerative approaches. Scaffolds have been developed that provide a controlled, sustained delivery of protein and non-viral DNA. Polymeric scaffolds capable of sustained drug release can be fabricated by the assembly of drug-loaded microspheres and subsequent fusion of the microspheres using a gas foaming process. This process has been developed to regulate the amount released and the release rates for protein, naked DNA, and DNA complexed with cationic lipid and polymers. Alternatively, we have developed a controlled DNA delivery system based on the tethering of DNA complexes to a biomaterial surfaces. Complexes can be immobilized at high densities and cells cultured on the substrates can internalize and express the DNA. These systems for the controlled delivery of proteins, or DNA-encoding for these proteins, are versatile and can be readily applied to the regeneration of various tissues.","PeriodicalId":60385,"journal":{"name":"中国地球物理学会年刊","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2002-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82710009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microarray analysis of the chondrocytic cell line T/C-28a2 under dynamic fluid shear 动态流体剪切作用下T/C-28a2软骨细胞微阵列分析

中国地球物理学会年刊 Pub Date : 2002-12-01 DOI: 10.1109/IEMBS.2002.1137001

J. Abulencia, R. Gaspard, John Quackenbush, K. Konstantopoulos

{"title":"Microarray analysis of the chondrocytic cell line T/C-28a2 under dynamic fluid shear","authors":"J. Abulencia, R. Gaspard, John Quackenbush, K. Konstantopoulos","doi":"10.1109/IEMBS.2002.1137001","DOIUrl":"https://doi.org/10.1109/IEMBS.2002.1137001","url":null,"abstract":"The behavior of the chondrocytic cell line T/C-28a2 under shear flow was examined using a 32,448 element microarray. A parallel plate flow chamber was used to generate a shear stress level of 20 dyn/cm/sup 2/ for 1.5 or 24 hours (h), after which gene regulation was measured. Microarray analysis revealed differentially regulated genes affecting proliferation/differentiation, extracellular matrix/cytoskeleton, and inflammation at both time points. A ribonuclease protection assay was performed on a subset of genes to confirm the data obtained from the microarray. However, the cyclooxygenase-2 (COX-2) gene, which plays a role in the prostaglandin production in inflamed tissues and the synovium of rheumatoid arthritis (RA) patients, was studied further. Western hybridization revealed that COX-2 protein is present at 24 h, but not at 6 or 12 h. Also, immunofluorescence microscopy shows that COX-2 protein localizes in the cytosol after 24 h of shear, and is not present after 1.5 h. By examining the overall gene expression profiles of chondrocytes under different conditions of dynamic fluid shear, new insights on the pathogenesis of cartilage related diseases such as rheumatoid arthritis might be generated.","PeriodicalId":60385,"journal":{"name":"中国地球物理学会年刊","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2002-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82504288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction of motion artifact in cardiac optical mapping using image registration technique 利用图像配准技术校正心脏光学成像中的运动伪影

中国地球物理学会年刊 Pub Date : 2002-12-01 DOI: 10.1109/IEMBS.2002.1106437

G.K. Rohde, V. Sidorov, A. Aldroubi, B. Dawant, Shien‐Fong Lin

引用次数: 2

Integration of the "How people learn" framework into educational module development and implementation in biotechnology 将“人如何学习”框架整合到生物技术教育模块的开发和实施中

中国地球物理学会年刊 Pub Date : 2002-12-01 DOI: 10.1109/IEMBS.2002.1053468

G. Birol, A. McKenna, H. Smith, T. Giorgio, S. Brophy

引用次数: 9

The effect of shock absorbing prosthetic components on the gaits of persons with lower-extremity amputations 减震假体对下肢截肢者步态的影响

中国地球物理学会年刊 Pub Date : 2002-12-01 DOI: 10.1109/IEMBS.2002.1053339

S. Gard, Regina J. Konz