生物物理学报:英文版最新文献_第5页

Preparation of Mycobacterium smegmatis porin A (MspA) nanopores for single molecule sensing of nucleic acids. 耻垢分枝杆菌孔蛋白A (MspA)单分子核酸传感纳米孔的制备。

生物物理学报:英文版 Pub Date : 2021-10-31 DOI: 10.52601/bpr.2021.210016

Yuqin Wang, Pingping Fan, Shanyu Zhang, Shuanghong Yan, Shuo Huang

引用次数: 1

Protein folding mechanism revealed by single-molecule force spectroscopy experiments. 单分子力谱实验揭示蛋白质折叠机制。

生物物理学报:英文版 Pub Date : 2021-10-31 DOI: 10.52601/bpr.2021.210024

Hao Sun, Zilong Guo, Haiyan Hong, Ping Yu, Zhenyong Xue, Hu Chen

{"title":"Protein folding mechanism revealed by single-molecule force spectroscopy experiments.","authors":"Hao Sun, Zilong Guo, Haiyan Hong, Ping Yu, Zhenyong Xue, Hu Chen","doi":"10.52601/bpr.2021.210024","DOIUrl":"https://doi.org/10.52601/bpr.2021.210024","url":null,"abstract":"Force spectroscopy experiments use mechanical force as a control factor to regulate the folding and unfolding process of proteins. Atomic force microscopy has been widely used to study the mechanical stability of proteins, and obtained unfolding forces and unfolding distance of different proteins, while recently, more low force folding and unfolding measurements were done by optical tweezers and magnetic tweezers. Due to the relatively small distortion of the free energy landscape, low force measurements give the free energy landscape information over bigger conformational space. In this review, we summarize the results of force spectroscopy experiments on different proteins. The unfolding distance obtained at high forces by atomic force microscopy are mostly smaller than 2 nm, while the unfolding distances at low forces distribute over a larger range: from a negative value to more than 6 nm. The sizes of the transition states at low force are ~4 nm for most compact two-state globular proteins, which indicates that this transition state might be the general free energy barrier separating the unfolded state and the theoretically predicated molten globule state. Up to now, only a limited number of proteins has been studied at low forces. We expect that more and more proteins with different conformations will be studied at low forces to reveal the general protein folding mechanism.","PeriodicalId":59621,"journal":{"name":"生物物理学报:英文版","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10233387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9597322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Multiplexed single-molecule force spectroscopy for dissecting biophysical regulation of membrane receptors functions on live cells. 用于解剖活细胞上膜受体功能的生物物理调节的多路单分子力谱。

生物物理学报:英文版 Pub Date : 2021-10-31 DOI: 10.52601/bpr.2021.210022

Chenyi An, Wei Chen

{"title":"Multiplexed single-molecule force spectroscopy for dissecting biophysical regulation of membrane receptors functions on live cells.","authors":"Chenyi An, Wei Chen","doi":"10.52601/bpr.2021.210022","DOIUrl":"https://doi.org/10.52601/bpr.2021.210022","url":null,"abstract":"Complex physical cues including two-dimensional membrane environment, dynamic mechanical force, and bioelectric activity inevitably affect membrane receptor functions. Multiplexed single-molecule force spectroscopy (SMFS) techniques with the capability of live-cell measurements are essential to systemically dissect receptor's functions under complex biophysical regulation. In this review, we summarize recent progress of live-cell based SMFS techniques and specifically focus on the progress of SMFS on the biomembrane force probe with enhanced mechanical stability and multiplexed capability of fluorescence imaging. We further suggest the necessity of developing multiplexed SMFS techniques with simultaneous bioelectric regulation capability to investigate membrane potential regulated membrane receptor functions. These state-of-art multiplexed SMFS techniques will dissect membrane receptors functions in a systematic biophysical angle, resolving the biochemical, biomechanical and bioelectrical regulatory mechanisms in physiologically relevant conditions.","PeriodicalId":59621,"journal":{"name":"生物物理学报:英文版","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10233390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9603182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Recent progress on single-molecule localization microscopy. 单分子定位显微镜研究进展。

生物物理学报:英文版 Pub Date : 2021-10-31 DOI: 10.52601/bpr.2021.210023

Lusheng Gu, Wei Ji

引用次数: 0

Intracellular transport dynamics revealed by single-particle tracking. 单粒子跟踪揭示的细胞内运输动力学。

生物物理学报:英文版 Pub Date : 2021-10-31 DOI: 10.52601/bpr.2021.210035

Ming-Li Zhang, Hui-Ying Ti, Peng-Ye Wang, Hui Li

引用次数: 6

Studying structure and functions of cell membranes by single molecule biophysical techniques. 利用单分子生物物理技术研究细胞膜的结构和功能。

生物物理学报:英文版 Pub Date : 2021-10-31 DOI: 10.52601/bpr.2021.210018

Qingrong Zhang, Siying Li, Yu Yang, Yuping Shan, Hongda Wang

{"title":"Studying structure and functions of cell membranes by single molecule biophysical techniques.","authors":"Qingrong Zhang, Siying Li, Yu Yang, Yuping Shan, Hongda Wang","doi":"10.52601/bpr.2021.210018","DOIUrl":"https://doi.org/10.52601/bpr.2021.210018","url":null,"abstract":"Cell membranes are complicated multicomponent structures, related to many basic cellular processes, such as substance transporting, energy conversion, signal transduction, mechanosensing, cell adhesion and so on. However, cell membranes have long been difficult to study at a single-molecule level due to their complex and dynamic properties. During the last decades, biophysical imaging techniques, such as atomic force microscopy and super-resolution fluorescent microscopy, have been developed to study biological structures with unprecedented resolution, enabling researchers to analyze the composition and distribution of membrane proteins and monitor their specific functions at single cell/molecule level. In this review, we highlight the structure and functions of cell membranes based on up-to-date biophysical techniques. Additionally, we describe the recent advances in force-based detecting technology, which allow insight into dynamic events and quantitativelymonitoring kinetic parameters for trans-membrane transporting in living cells.","PeriodicalId":59621,"journal":{"name":"生物物理学报:英文版","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10233388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9591433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Mutual interaction of microbiota and host immunity during health and diseases. 健康和疾病期间微生物群与宿主免疫的相互作用。

生物物理学报:英文版 Pub Date : 2021-08-31 DOI: 10.52601/bpr.2021.200045

Di Wu, Yinlian Zhang, Suwei Dong, Chao Zhong

{"title":"Mutual interaction of microbiota and host immunity during health and diseases.","authors":"Di Wu, Yinlian Zhang, Suwei Dong, Chao Zhong","doi":"10.52601/bpr.2021.200045","DOIUrl":"https://doi.org/10.52601/bpr.2021.200045","url":null,"abstract":"Microbiota-host interaction has attracted more and more attentions in recent years. The association between microbiota and host health is largely attributed to its influence on host immune system. Microbial-derived antigens and metabolites play a critical role in shaping the host immune system, including regulating its development, activation, and function. However, during various diseases the microbiota-host communication is frequently found to be disordered. In particular, gut microbiota dysbiosis associated with or led to the occurrence and progression of infectious diseases, autoimmune diseases, metabolic diseases, and neurological diseases. Pathogenic microbes and their metabolites disturb the protective function of immune system, and lead to disordered immune responses that usually correlate with disease exacerbation. In the other hand, the immune system also regulates microbiota composition to keep host homeostasis. Here, we will discuss the current advances of our knowledge about the interactions between microbiota and host immune system during health and diseases.","PeriodicalId":59621,"journal":{"name":"生物物理学报:英文版","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10233470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9597282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Adaptive optics in super-resolution microscopy. 超分辨率显微镜中的自适应光学。

生物物理学报:英文版 Pub Date : 2021-08-31 DOI: 10.52601/bpr.2021.210015

Jingyu Wang, Yongdeng Zhang

引用次数: 2

Ferroptosis and its emerging role in tumor. 脱铁症及其在肿瘤中的新作用。

生物物理学报:英文版 Pub Date : 2021-08-31 DOI: 10.52601/bpr.2021.210010

Xiaoxuan Wang, Zicheng Liu, Lijuan Ma, Haijie Yu

{"title":"Ferroptosis and its emerging role in tumor.","authors":"Xiaoxuan Wang, Zicheng Liu, Lijuan Ma, Haijie Yu","doi":"10.52601/bpr.2021.210010","DOIUrl":"10.52601/bpr.2021.210010","url":null,"abstract":"Ferroptosis is a novel form of programmed cell death characterized by iron-dependent lipid peroxidation accumulation. It is morphologically, biochemically, and genetically distinct from other known cell death, such as apoptosis, necrosis, and pyroptosis. Its regulatory mechanisms include iron metabolism, fatty acid metabolism, mitochondrial respiration, and antioxidative systems eliminating lipid peroxidation, such as glutathione synthesis, selenium-dependent glutathione peroxidase 4, and ubiquinone. The disruption of cellular redox systems causes damage to the cellular membrane leading to ferroptotic cell death. Recent studies have shown that numerous pathological diseases, like tumors, neurodegenerative disorders, and ischemia-reperfusion injury are associated with ferroptosis. As such, pharmacological regulation of ferroptosis either by activation or by suppression will provide a vast potential for treatments of relevant diseases. This review will discuss the advanced progress in ferroptosis and its regulatory mechanisms from both the antioxidative and oxidative sides. In addition, the roles of ferroptosis in various tumorigenesis, development, and therapeutic strategies will be addressed, particularly to chemotherapy and immunotherapy, as well as the discoveries from Traditional Chinese Medicine. This review will lead us to have a comprehensive understanding of the future exploration of ferroptosis and cancer therapy.","PeriodicalId":59621,"journal":{"name":"生物物理学报:英文版","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10233469/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9597284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Instant multicolor super-resolution microscopy with deep convolutional neural network. 即时多色超分辨率显微镜与深度卷积神经网络。

生物物理学报:英文版 Pub Date : 2021-08-31 DOI: 10.52601/bpr.2021.210017

Songyue Wang, Chang Qiao, Amin Jiang, Di Li, Dong Li

{"title":"Instant multicolor super-resolution microscopy with deep convolutional neural network.","authors":"Songyue Wang, Chang Qiao, Amin Jiang, Di Li, Dong Li","doi":"10.52601/bpr.2021.210017","DOIUrl":"https://doi.org/10.52601/bpr.2021.210017","url":null,"abstract":"Multicolor super-resolution (SR) microscopy plays a critical role in cell biology research and can visualize the interactions between different organelles and the cytoskeleton within a single cell. However, more color channels bring about a heavier budget for imaging and sample preparation, and the use of fluorescent dyes of higher emission wavelengths leads to a worse spatial resolution. Recently, deep convolutional neural networks (CNNs) have shown a compelling capability in cell segmentation, super-resolution reconstruction, image restoration, and many other aspects. Taking advantage of CNN's strong representational ability, we devised a deep CNN-based instant multicolor super-resolution imaging method termed IMC-SR and demonstrated that it could be used to separate different biological components labeled with the same fluorophore, and generate multicolor images from a single super-resolution image in silico. By IMC-SR, we achieved fast three-color live-cell super-resolution imaging with ~100 nm resolution over a long temporal duration, revealing the complicated interactions between multiple organelles and the cytoskeleton in a single COS-7 cell.","PeriodicalId":59621,"journal":{"name":"生物物理学报:英文版","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10233468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9590938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1