Gamin Kim, Ho Jeong Jeong, Sook-Young Kim, Gi Dong Han
{"title":"Anti-adipogenic and antioxidant activities of spent coffee grounds extracts: impacts of chlorogenic acid and caffeic acid","authors":"Gamin Kim, Ho Jeong Jeong, Sook-Young Kim, Gi Dong Han","doi":"10.1007/s10068-025-01886-z","DOIUrl":"10.1007/s10068-025-01886-z","url":null,"abstract":"<div><p>The rise in global coffee consumption has increased spent coffee grounds (SCG), a by-product rich in bioactive compounds. This study compared the anti-adipogenic and antioxidant activities of SCG extracts before (SCG) and after fermentation (FSCG) with <i>Aspergillus oryzae</i>. SCG showed higher antioxidant activity and total polyphenol content than FSCG. In vitro studies on 3T3-L1 adipocytes revealed that SCG significantly inhibited lipid accumulation and downregulated key adipogenic markers (PPARγ, C/EBPα, adiponectin) in a dose-dependent manner, while FSCG had limited effects. Thin-layer chromatography indicated fermentation decreased chlorogenic acid and increased caffeic acid, suggesting structural changes in bioactive compounds. The superior anti-adipogenic effect of SCG is likely due to chlorogenic acid, which degrades during fermentation. These findings highlight SCG’s potential as a functional food ingredient with anti-obesity and antioxidant properties.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 11","pages":"2635 - 2642"},"PeriodicalIF":3.1,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Impact of cooking methods on the oxidative stability of fish balls enriched with solvent-specific antioxidant sources","authors":"Jiyea Lee, Jeonghee Surh","doi":"10.1007/s10068-025-01882-3","DOIUrl":"10.1007/s10068-025-01882-3","url":null,"abstract":"<div><p>This study evaluated the effects of various cooking methods on the oxidative stability of fish balls, used as a model for oxidizable food, enriched with antioxidant sources: cacao powder (CP), exhibiting higher activity in polar solvents, and ginger powder (GP), showing higher activity in non-polar solvents. CP or GP was incorporated at equivalent antioxidant levels, and the fish balls were prepared using boiling, steaming, air-frying, deep-frying, oven-roasting, or microwave cooking. Cooking reduced antioxidant activity to 20% and raised lipid oxidation products (LOP) up to 3.3-fold depending on the method. During frozen storage, LOP formation was lower in cooked fish balls compared to uncooked counterparts, with GP demonstrating superior effectiveness in mitigating the effects of different cooking methods. These findings indicate that cooking influences the oxidative stability of fish balls and that non-polar antioxidants, such as GP, are more effective than polar antioxidants, such as CP, in enhancing lipid oxidative stability.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 and Processing in Home and Industry","pages":"2979 - 2988"},"PeriodicalIF":3.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144914613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jinwoo Kim, Jiwoon Kim, Gyungcheon Kim, Seongok Kim, Hakdong Shin
{"title":"Prebiotic potential of wheat and dark rye: modulation of gut microbiota and short-chain fatty acid-producing bacteria","authors":"Jinwoo Kim, Jiwoon Kim, Gyungcheon Kim, Seongok Kim, Hakdong Shin","doi":"10.1007/s10068-025-01889-w","DOIUrl":"10.1007/s10068-025-01889-w","url":null,"abstract":"<div><p>The consumption of whole grains supports metabolic health in the gut and reduces the risk of chronic diseases. Dietary fiber derived from whole grains is one of the key factors contributing to these benefits. These fibers act as prebiotics, promoting the growth of beneficial bacteria and shaping microbial composition in the gut. This study examined the effects of wheat and dark rye, two fiber-rich whole grains, on the gut microbiota using an in vitro fecal incubation system. Following whole grain treatment, the gut microbiota showed an increase in the relative abundances of <i>Bifidobacterium</i>, <i>Faecalibacterium</i>, <i>Blautia</i>, and <i>Lachnospiraceae</i>. These bacteria are known short-chain fatty acid producers, suggesting a potential role in supporting host health. Additionally, functional pathway analysis predicted the upregulation of metabolic pathways related to specific carbohydrate metabolism. Taken together, these findings enhance our understanding of how wheat and dark rye consumption influences gut microbiota composition.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 11","pages":"2563 - 2570"},"PeriodicalIF":3.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolation and identification of the lactase (β-galactosidase) from the soil bacterium Weizmannia coagulans and its expression and production in the expression system of Escherichia coli","authors":"Xiuling Li, Tiantian Wang, Zhenzhu Shao, Jiajia Yin, Tengyu Ma, Yu Tian, Hongguang Zhu, Haixin Sun","doi":"10.1007/s10068-025-01891-2","DOIUrl":"10.1007/s10068-025-01891-2","url":null,"abstract":"<div><p>Lactase plays an important role in biotechnology and food industry, and the development of a lactase with high enzymatic activity and stability is the key to its industrial application. In this study, we isolated a lactase-producing strain from a dairy farm raw milk storage room. Whole genome sequencing analysis showed that the strain was <i>Weizmannia coagulans</i>, named <i>Weizmannia coagulans</i> LXL310-1, with a lactase activity of 0.817 U/mL. The lactase gene <i>bgaB</i> from <i>W. coagulans</i> was amplified by PCR and expressed in an <i>Escherichia coli</i> expression system. The enzyme was further purified following fermentation using ammonium sulfate precipitation, gel filtration and ion exchange chromatography. Lactase activity was enhanced 72.6 fold to 59.35 U/mL using this heterologous expression system. The lactase activity obtained in this study reached a high level. This study successfully established a systematic lactase recombinant expression system to provide a foundation for industrial lactase production and further theoretical investigations.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 12","pages":"2831 - 2841"},"PeriodicalIF":3.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144625195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuncheong Choi, Ji Sou Lyu, Jung-Soo Lee, Yoonjee Chang, Jaejoon Han
{"title":"Industrial-scale antifungal film incorporated with Cinnamomum cassia oil: enhancing bread quality attributes and evaluation of organoleptic properties","authors":"Yuncheong Choi, Ji Sou Lyu, Jung-Soo Lee, Yoonjee Chang, Jaejoon Han","doi":"10.1007/s10068-025-01883-2","DOIUrl":"10.1007/s10068-025-01883-2","url":null,"abstract":"<div><p>This research aims to develop an antifungal packaging film to mitigate the quality deterioration of mass-produced bread for industrial purposes. Various antifungal agents were tested against four target fungi; <i>Aspergillus niger</i>, <i>A. flavus</i>, <i>Penicillium corylophilum</i>, and <i>Neurospora</i> sp.; commonly found in bread, to replace the commercial antifungal film. Among them, <i>Cinnamomum cassia</i> essential oil (CEO) exhibited strong antifungal activity, with MIC values below 100 ppm against all tested strains. We implemented the CEO-incorporated powder onto the multilayered packaging film and subsequent packaging trials were conducted on the industrial packaging machinery. Organoleptic analyses using electronic tongue and electronic nose revealed that the application of CEO maintained the bread’s flavor and aroma throughout storage, implying the newly developed CEO-treated film was comparably preferred to the conventional material. Therefore, this investigation highlights the promising potential of CEO’s effective antifungal agent to combat bread quality deterioration during distribution for industrial operations.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 11","pages":"2519 - 2528"},"PeriodicalIF":3.1,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kyeong-Ok Choi, Cheul-Soon Yim, Phu Cuong Nguyen, Jong-Tae Park
{"title":"Structural and functional variations of octenyl succinylated clustered amylopectin produced by distinctive glycogen branching enzymes","authors":"Kyeong-Ok Choi, Cheul-Soon Yim, Phu Cuong Nguyen, Jong-Tae Park","doi":"10.1007/s10068-025-01892-1","DOIUrl":"10.1007/s10068-025-01892-1","url":null,"abstract":"<div><p>Clustered amylopectins (CAPs) were produced using recombinant glycogen branching enzymes from <i>Escherichia coli</i> K12 (EcGBE) and <i>Vibrio vulnificus</i> (VvGBE). The CAPs were esterified with octenyl succinic anhydride (OSA), introducing novel functional characteristics. Vv-CAP demonstrated a reduced apparent molecular weight and a higher prevalence of both short (degree of polymerization (DP) ≤ 5, 13.3%) and medium branches (5 < DP ≤ 12, 58.2%) compared to Ec-CAP. FT-IR spectra confirmed the successful esterification of CAPs with OSA. <sup>1</sup>H-NMR analysis showed the degree of substitution (DS) for OSA-CAPs varied based on the CAP to OSA ratio (DS 0.021–0.058 for Ec-CAP and 0.011–0.066 for Vv-CAP). Esterification with OSA on shortened branches resulted in a slower digestion rate and increased resistant starch proportion. Additionally, this process enhanced the emulsifying properties of CAPs and improved the aqueous solubility of puerarin. OSA-modified CAPs are promising for pharmaceutical applications.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 11","pages":"2445 - 2453"},"PeriodicalIF":3.1,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of rice protein on the retrogradation of waxy rice starch gels","authors":"Manhee Baek, Semin Jeong, Sujin Jeon, Saehun Mun","doi":"10.1007/s10068-025-01887-y","DOIUrl":"10.1007/s10068-025-01887-y","url":null,"abstract":"<div><p>This study investigated the effects of rice protein (RP) addition on the retrogradation of waxy rice starch (WRS) gels during storage. The retrogradation behavior was evaluated using a rapid visco analyser, a differential scanning calorimeter, an X-ray diffractometer, a texture analyser, and a scanning electron microscope. The incorporation of RP inhibited the swelling of WRS, leading to reductions in trough viscosities, as well as setback values. DSC analysis indicated a reduction in melting enthalpy, while XRD showed decreased peak intensity in retrograded WRS with RP addition. After 3 days of storage at 4 °C, gel hardness increased, but RP significantly mitigated this effect. SEM observations showed that a 5% RP addition produced larger pore sizes and a looser network structure, supporting the inhibition of retrogradation. These findings suggest that RP effectively retards the retrogradation of WRS, offering potential applications in enhancing the texture and shelf-life of starch-based food products.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 11","pages":"2507 - 2518"},"PeriodicalIF":3.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hang Li, Mengyuan Li, Manman Zhang, Ruixue Liu, Nan Li, Yongsheng Wang, Zhihan Wang, Shihan Wang
{"title":"Waste valorization of Rana chensinensis ovum protein isolates versus commercially available proteins isolates from physicochemical, structural, and functional properties to stabilizing Pickering emulsions","authors":"Hang Li, Mengyuan Li, Manman Zhang, Ruixue Liu, Nan Li, Yongsheng Wang, Zhihan Wang, Shihan Wang","doi":"10.1007/s10068-025-01870-7","DOIUrl":"10.1007/s10068-025-01870-7","url":null,"abstract":"<div><p>This study demonstrated the commercialization potential of <i>Rana chensinensis</i> ovum protein isolates (RCOPI) by comparing it with commercially available proteins from physicochemical, structural, and functional properties to stabilizing Pickering emulsions. The size of RCOPI colloids nanoparticles reached 65 nm which is much smaller than the commercially available proteins. Besides, RCOPI exhibited incredible emulsion ability with the emulsion drop around 22 μm diameters and the zeta potential approximately − 50 mV. The emulsions stabilized by RCOPI showed remarkable stability and the Lactalysis index was about 21% after 100 days storage. In the encapsulation test, scanning electron microscopy (SEM) showed the size of the microcapsule ranging from 20 to 50 μm. The encapsulated efficiency of RCOPI stabilized emulsion was as good as the compared proteins, up to 75%. Furthermore, the results of the sensory evaluation showed that RCOPI stabilized emulsions received the highest approval ratings for appearance, taste, flavor and overall acceptability.</p><h3>Graphic Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 11","pages":"2455 - 2467"},"PeriodicalIF":3.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Supercritical fluid treatment for egg white protein modification: a comprehensive review","authors":"Syed Mansha Rafiq, Reshab Majumder, Deepak Joshi, Soumita Bag, Aamir Hussain Dar, Kshirod Kumar Dash, Urba Shafiq Sidiqi","doi":"10.1007/s10068-025-01856-5","DOIUrl":"10.1007/s10068-025-01856-5","url":null,"abstract":"<div><p>Supercritical fluid technology is an innovative technique that has sparked interest in its potential for structural alteration since it utilizes less difficult-to-recycle compounds such as salts, acids, and bases in the protein industry’s processing. It may become a novel method of protein modification in the food sector due to its abundant source, low cost, eco-friendliness. This work presents a comprehensive review of supercritical fluid technologies, focussing on their application in the innovative area of supercritical fluid therapy, specifically examining the modification of egg white protein. Although this technique offers several advantages, there are multiple issues to be taken forward which includes scalability, cost-effectiveness, safety considerations and regulatory aspects when applying commercially especially food and medical uses. Research on the effect of molecular modifications on the functional properties of egg white proteins is greatly promising.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 15","pages":"3507 - 3525"},"PeriodicalIF":3.1,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145296833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}