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Cattle dung detection in pastures from drone images using YOLOv5 利用 YOLOv5 从无人机图像中检测牧场中的牛粪
IF 1.1 4区 农林科学
Grassland Science Pub Date : 2024-09-15 DOI: 10.1111/grs.12429
Kensuke Kawamura, Yura Kato, Taisuke Yasuda, Eriko Aozasa, Masato Yayota, Miya Kitagawa, Kyoko Kunishige
{"title":"Cattle dung detection in pastures from drone images using YOLOv5","authors":"Kensuke Kawamura,&nbsp;Yura Kato,&nbsp;Taisuke Yasuda,&nbsp;Eriko Aozasa,&nbsp;Masato Yayota,&nbsp;Miya Kitagawa,&nbsp;Kyoko Kunishige","doi":"10.1111/grs.12429","DOIUrl":"https://doi.org/10.1111/grs.12429","url":null,"abstract":"<p>Livestock excretions are crucial for nutrient cycling in pasture ecosystems. However, conventional methods based on field observations require significant human power and are time-consuming. This study developed a model, ‘Dung Detector (DD)’, for detecting cattle dung in pastures from drone images using the You Only Look Once (YOLO) v5 algorithm. The DD model was trained using our custom dataset including 1,504 split images from drone orthomosaic images in five paddocks: Obihiro (OBH), Shintoku (STK), Minokamo (MNO), Miyota (MYT), and Yatsugatake (YGK). The detection accuracy was evaluated using ground-truth data acquired in two quadrats within paddocks. The DD model performed well for OBH and STK (<i>F</i>-score = 0.861 and 0.835) paddocks with simple grass species and low surface sward height (SSH). Although the MNO and MYT, with complex vegetation and high SSH, showed few false positives (precision &gt;0.9), some cattle dung pats were undetectable, presumably due to grass height (Recall = 0.500 and 0.276).</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"70 4","pages":"168-174"},"PeriodicalIF":1.1,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142404620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Potassium fertilization and defoliation intensity effects on forage characteristics of “BRS Zuri” guineagrass 钾肥和落叶强度对 "BRS Zuri "石竹牧草特性的影响
IF 1.1 4区 农林科学
Grassland Science Pub Date : 2024-09-10 DOI: 10.1111/grs.12431
Stefane Cunha, Joao M. B. Vendramini, Joao Lazarin, Antonio C. Dos Santos, Maria L. Silveira, Fabricia R. C. Miotto, Nayara M. Alencar, Philipe Moriel, Jose Carlos B. Dubeux Jr
{"title":"Potassium fertilization and defoliation intensity effects on forage characteristics of “BRS Zuri” guineagrass","authors":"Stefane Cunha,&nbsp;Joao M. B. Vendramini,&nbsp;Joao Lazarin,&nbsp;Antonio C. Dos Santos,&nbsp;Maria L. Silveira,&nbsp;Fabricia R. C. Miotto,&nbsp;Nayara M. Alencar,&nbsp;Philipe Moriel,&nbsp;Jose Carlos B. Dubeux Jr","doi":"10.1111/grs.12431","DOIUrl":"10.1111/grs.12431","url":null,"abstract":"<p>The objective of this study was to evaluate the effects of different potassium (K) fertilization levels and defoliation intensities on performance of “BRS Zuri” guineagrass <i>Megathyrsus maximus</i> (Jacq.) B.K. Simon &amp; S.W.L Jacobs (syn. <i>Panicum maximum</i> Jacq.). Treatments were the factorial arrangement of three K fertilization levels: 0 (control), 40, 80 kg K<sub>2</sub>O/ha/harvest) and two defoliation intensities (10- and 20-cm stubble heights), distributed in a completely randomized design with five replications. The experiment was conducted in Araguaina, TO, Brazil, from October to January, 2020–2021 and 2021–2022. Plots were harvested four times per year with 21-day regrowth interval. The 40 and 80 kg K<sub>2</sub>O/ha/harvest fertilization levels had greater HA than the control (5490 vs. 3,440 kg DM/ha, respectively). There was a K fertilization level × stubble height interaction on tiller population. There was greater tiller population at 20- than 10-cm stubble height at 40 kg K<sub>2</sub>O/ha/harvest; however, no effect of stubble height was detected at 0 and 80 kg K<sub>2</sub>O/ha/harvest. Plots fertilized with 80 kg K<sub>2</sub>O/ha/harvest had greater tiller population than 40 kg K<sub>2</sub>O/ha/harvest, which was greater than control. Forage harvested at 10 cm had greater crude protein (CP) than 20-cm stubble height (mean = 11.4% vs. 10.8%). Tissue K concentration increased from control to 40 kg K<sub>2</sub>O/ha/harvest, but there was no difference between 40 and 80 kg K<sub>2</sub>O/ha/harvest (mean = 1.1% vs. 3.5%). The combination of 40 kg K<sub>2</sub>O/ha/harvest and 20-cm stubble height may be a desirable management strategy to promote Zuri guineagrass productivity in tropical regions.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"70 4","pages":"160-167"},"PeriodicalIF":1.1,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142189209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phylogenomic identification and overexpression of plant size–related genes in Setaria viridis and rice 藤黄属植物和水稻中植物大小相关基因的系统发生组鉴定和过表达
IF 1.1 4区 农林科学
Grassland Science Pub Date : 2024-09-03 DOI: 10.1111/grs.12430
Chudamani Sharma Prakash, Jieqin Li, Paul W. Bible, Carina A. Collins, Wenmiao Tu, Jingyi Xu, Yi-Hong Wang
{"title":"Phylogenomic identification and overexpression of plant size–related genes in Setaria viridis and rice","authors":"Chudamani Sharma Prakash,&nbsp;Jieqin Li,&nbsp;Paul W. Bible,&nbsp;Carina A. Collins,&nbsp;Wenmiao Tu,&nbsp;Jingyi Xu,&nbsp;Yi-Hong Wang","doi":"10.1111/grs.12430","DOIUrl":"10.1111/grs.12430","url":null,"abstract":"<p>Plant size is a critical component of agricultural productivity as larger plants produce more biomass. To identify genes related to plant size, we grouped C<sub>4</sub> grasses into small and large and used OrthoFinder to find orthologous genes present in large but absent in small grasses. Three such genes were identified from sorghum (<i>Sorghum bicolor</i> [L.] Moench) by phylogenomic approach, and they encode nitrate transporter (Sobic.007G213200), oxysterol binding protein (SbRio.01G578800) and thioredoxin reductase (SbRio.05G168300), respectively. Overexpression of all three genes driven by the maize ubiquitin promoter in <i>Setaria viridis</i> (L.) Beauv. indicates that they all affected plant size as measured by plant height and tiller number. Both nitrate transporter and oxysterol binding protein increased plant height and tiller number, and thioredoxin reductase significantly decreased tiller number but had minimal effect on plant height. In rice (<i>Oryza sativa</i> L.), all three constructs reduced plant height significantly. The only commonality between the transgenic species was that nitrate transporter and oxysterol binding protein increased tiller number in both <i>S. viridis</i> and rice. Overall, we have demonstrated that phytogenomic approach can be used to identify genes responsible for large plant size in the grasses.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"70 4","pages":"151-159"},"PeriodicalIF":1.1,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142189219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differences of morphological and physiological responses of sainfoin varieties/lines under simulated drought stresses 模拟干旱胁迫下红豆杉品种/品系的形态和生理反应差异
IF 1.1 4区 农林科学
Grassland Science Pub Date : 2024-06-24 DOI: 10.1111/grs.12428
Yuheng Yao, Kun Wang, Lili Nan
{"title":"Differences of morphological and physiological responses of sainfoin varieties/lines under simulated drought stresses","authors":"Yuheng Yao,&nbsp;Kun Wang,&nbsp;Lili Nan","doi":"10.1111/grs.12428","DOIUrl":"10.1111/grs.12428","url":null,"abstract":"<p>Sainfoin (<i>Onobrychis viciaefolia</i>) is an important legume forage. This study aims to explore the response mechanisms of sainfoin germplasm resource accessions to drought stress, which provides the theoretical basis for tolerant breeding of sainfoin. In this study, 20 sainfoin germplasm resource accessions were used as the test materials, and the effects of drought stress on morphological and physiological characteristics were determined under simulated levels of drought (−0.5, −1.0, −1.5 and −2.0 MPa) × exposure times (7 days) in pots. The results showed that leaf area, leaf relative water content, root-shoot ratio, root length, root surface area, root volume, root diameter, root tip number, chlorophyll a content, chlorophyll b content and catalase activity decreased with increasing drought stress. In contrast, proline, soluble sugar, soluble protein and malondialdehyde contents increased in response to drought. Root activity, superoxide dismutase and peroxidase activities increased first and then decreased and reached the maximum at −1.0 MPa. Cluster analysis showed that 20 sainfoin germplasm resources could be divided into three categories, of which four accessions were high tolerance types, and three accessions were low tolerance type. These findings will help provide some theoretical basis for cultivating new varieties.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"70 4","pages":"133-150"},"PeriodicalIF":1.1,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141510341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Panicum maximum cultivars for use in integrated agricultural production systems in Cerrado biome soils 用于塞拉多生物群落土壤综合农业生产系统的最大秫米栽培品种
IF 1.1 4区 农林科学
Grassland Science Pub Date : 2024-05-12 DOI: 10.1111/grs.12423
Lucas Ruaro Bublitz, Antonio Leandro Chaves Gurgel, Alexandre César Mauri, Vitor Cardoso Queiroz, Kermilly de Souza Lima, Itália Bianca Reis Campelo, Marcos Jácome de Araújo, Tairon Pannunzio Dias-Silva, Juliana da Silva Barros, Isadora Osório Maciel Aguiar, Gelson dos Santos Difante, Luís Carlos Vinhas Ítavo
{"title":"Panicum maximum cultivars for use in integrated agricultural production systems in Cerrado biome soils","authors":"Lucas Ruaro Bublitz,&nbsp;Antonio Leandro Chaves Gurgel,&nbsp;Alexandre César Mauri,&nbsp;Vitor Cardoso Queiroz,&nbsp;Kermilly de Souza Lima,&nbsp;Itália Bianca Reis Campelo,&nbsp;Marcos Jácome de Araújo,&nbsp;Tairon Pannunzio Dias-Silva,&nbsp;Juliana da Silva Barros,&nbsp;Isadora Osório Maciel Aguiar,&nbsp;Gelson dos Santos Difante,&nbsp;Luís Carlos Vinhas Ítavo","doi":"10.1111/grs.12423","DOIUrl":"10.1111/grs.12423","url":null,"abstract":"<p>Integrated production systems have been an alternative for diversifying agricultural production, and therefore, it is necessary to study tropical forage grasses that can impact both straw production and soil organic matter, as well as benefit animal production. The objective of this work was to evaluate the productive characteristics and accumulation of nutrients in the biomass of grasses of the genus <i>Panicum</i> grown in the off-season, in Cerrado biome soil as cover plants for use in integrated production systems. It evaluated different grasses of <i>Panicum maximum</i>: Aruana, Tamani, and Massai guinea grasses, distributed in a randomized block design with four replications of each grass. The productive characteristics, mineral accumulation, and carbon/nitrogen (C/N) ratio in the biomass of the cultivars were evaluated. No cultivar effect was observed for forage mass (3997.4 kg/ha DM). However, the largest (<i>p =</i> 0.0077) leaf blade masses were observed in the Massai and Tamani guinea grasses. On the contrary, the Aruana guinea grass exhibited the highest value (<i>p =</i> 0.0001) for stem mass, reflected in a higher (<i>p =</i> 0.0001) leaf/stalk ratio in the Massai and Tamani guinea grasses. No effect (<i>p &gt;</i> 0.05) of the cultivar on micronutrients, magnesium, and phosphorus concentrations was observed. However, the Aruana guinea grass presented higher concentrations of calcium and sulfur; on the other hand, potassium values were higher in the Massai and Tamani guinea grasses. The Massai guinea grass exhibited a higher (<i>p =</i> 0.0214) C/N ratio, while the Aruana guinea grass recorded lower values, with no significant differences between these cultivars and Tamani guinea grass. The Tamani and Massai guinea grasses stand out as recommended choices for cultivation during the off-season in Cerrado biome soils as cover crops in integrated production systems.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"70 3","pages":"121-129"},"PeriodicalIF":1.1,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140940381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spatial variation in grassland vegetation and herbage mass in the Ejina oasis along the Heihe River in the southern Gobi Desert, China 中国南戈壁滩黑河沿岸额济纳绿洲草原植被和草量的空间变化
IF 1.1 4区 农林科学
Grassland Science Pub Date : 2024-04-07 DOI: 10.1111/grs.12421
Masae Shiyomi, Jun Chen, Yasuo Yamamura, Yoshimichi Hori
{"title":"Spatial variation in grassland vegetation and herbage mass in the Ejina oasis along the Heihe River in the southern Gobi Desert, China","authors":"Masae Shiyomi,&nbsp;Jun Chen,&nbsp;Yasuo Yamamura,&nbsp;Yoshimichi Hori","doi":"10.1111/grs.12421","DOIUrl":"10.1111/grs.12421","url":null,"abstract":"<p>Our study area, Ejina, is located in the southern Gobi Desert, Inner Mongolia, China, where the annual precipitation is &lt;50 mm and the annual potential evaporation is &gt;3,600 mm. The Heihe River flows from the Qilian Range through the study area, and the desert landscape along the river includes oases formed by forests and grasslands. We established a survey area of approximately 1.5 ha in grassland facing a seasonal swamp, a branch of the Heihe River. We posed the following questions in this desert grassland: (1) how do soil water content (SWC) and salinity change with distance from the riverbank? (2) How do vegetation, aboveground biomass, and species richness change with distance from the riverbank? (3) How much can be supplied to grazing animals, and how many animals can be grazed? Our results indicated that (1) the SWC is high within 20 m of the riverbank and low beyond 20 m, the standing biomass is positively correlated with SWC, but there was no evidence of a correlation between salinity and distance to water; the electrical conductivity was high both inland and adjacent to the riparian swamp. (2) Vegetation could be classified to six classes, following a gradient from the riverbank (swamp margin) to the dry inland area beyond 20 m from the riverbank. Species richness and aboveground biomass were highly correlated. Classification result led us to recommend that generalist species tolerant of the distinct moisture gradient in the site be used in restoration efforts. (3) Per-hectare aboveground biomass was 478.93 dry weight (dw) kg, 321.2 ha of equivalent grassland would be required to graze 100 goats annually.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"70 3","pages":"95-108"},"PeriodicalIF":1.1,"publicationDate":"2024-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140561415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of drought stress on the functional traits and rhizosphere microbial community structure of Cyperus esculentus 干旱胁迫对香附功能特征和根瘤微生物群落结构的影响
IF 1.1 4区 农林科学
Grassland Science Pub Date : 2024-03-17 DOI: 10.1111/grs.12422
Binshuo Liu, Yunhang Hu, Ming Li, Honghai Xue, Ying Wang, Zhonghe Li
{"title":"Effects of drought stress on the functional traits and rhizosphere microbial community structure of Cyperus esculentus","authors":"Binshuo Liu,&nbsp;Yunhang Hu,&nbsp;Ming Li,&nbsp;Honghai Xue,&nbsp;Ying Wang,&nbsp;Zhonghe Li","doi":"10.1111/grs.12422","DOIUrl":"10.1111/grs.12422","url":null,"abstract":"<p>Drought is a major factor limiting plant growth. Plants cope with stress via morphophysiological responses. Rhizosphere-related bacteria, fungi and other microorganisms can help plants cope with drought via various mechanisms. We conducted a pot experiment on <i>Cyperus esculentus</i> wherein we subjected it to various drought levels and analyzed the functional traits of its aboveground and belowground organs. High-throughput sequencing was used to study the structure and diversity of the microbial community in the rhizosphere. Drought stress substantially lowered the densities of the leaves, stems, roots and seeds relative to the untreated control. Drought stress inhibited the growth and reduced the yield of <i>C. esculentus</i>. The leaves and seeds have higher water content and are more sensitive to drought stress than the roots. With the aggravation of drought stress, the plant height of <i>C. esculentus</i> decreased significantly, the root:shoot ratio increased and the specific leaf weight had no significant difference. Change in shoot height was the most evident response of <i>C. esculentus</i> to drought stress. Plants under drought stress reduced their leaf area and assumed a specific leaf weight to maintain photosynthetic performance. Rhizosphere fungi were more sensitive to drought stress than rhizosphere bacteria. Drought stress increased the relative abundances of <i>Bacteroidetes</i>, <i>Verrucomicrobia</i>, <i>Patescibacteria</i>, <i>Actinobacteria</i> and <i>Nitrospirae</i>. Drought-stressed <i>C. esculentus</i> maintained their photosynthetic performance by reducing shoot height and leaf area while assuming a stable specific leaf weight. Drought stress exerted a significantly stronger negative impact on rhizosphere fungi than rhizosphere bacteria. The results clarified the response strategies of functional traits and rhizosphere microorganisms of <i>C. esculentus</i> to drought stress.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"70 3","pages":"109-120"},"PeriodicalIF":1.1,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140168011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of cutting regime and genotype on growth, seed yield, seed quality and herbage yield of seven Urochloa (syn. Brachiaria) grass genotypes in the Adamawa region of Cameroon 喀麦隆阿达马瓦地区七种 Urochloa(同义 Brachiaria)草基因型的生长、种子产量、种子质量和牧草产量受切割制度和基因型的影响
IF 1.3 4区 农林科学
Grassland Science Pub Date : 2024-02-13 DOI: 10.1111/grs.12420
Ntane Ojong, Mandah Takor, Andrew Egbe, Eneke Bechem, Kingsley Etchu, Collins Mutai
{"title":"The effect of cutting regime and genotype on growth, seed yield, seed quality and herbage yield of seven Urochloa (syn. Brachiaria) grass genotypes in the Adamawa region of Cameroon","authors":"Ntane Ojong,&nbsp;Mandah Takor,&nbsp;Andrew Egbe,&nbsp;Eneke Bechem,&nbsp;Kingsley Etchu,&nbsp;Collins Mutai","doi":"10.1111/grs.12420","DOIUrl":"10.1111/grs.12420","url":null,"abstract":"<p>The performance of seven <i>Urochloa</i> grass genotypes (three worldwide cultivars cv. Basilisk, cv. Piata, cv. Xaraes and four landrace cv. Adamaoua1, cv. Adamaoua2, cv. North1 and cv. North2) subjected to two cutting regimes (mid-June cut and mid-July cut) was evaluated for regrowth characteristics, seed yields, seed quality and herbage production in 2020 and 2021 cropping seasons at Wakwa, Ngaoundere, Cameroon. This divergence in harvesting practices raises concerns regarding the effectiveness and efficiency of forage utilization, as well as the potential impact on livestock productivity and sustainable farming systems. A split plot design with five replications and a 1-year-old sward was used for the study. In both years, genotypes were significantly different for plant height and tiller numbers at 12 weeks after cutting (<i>P</i> ≤ 0.007). In 2020, the dry matter yield (DMY) ranged between 8.78 t/ha (cv. Piata) and 12.75 t/ha (cv. Xaraes) for June cut regrowth and between 7.69 t/ha (cv. Piata) and 10.26 t/ha (cv. Xaraes) for July cut regrowth. In 2021, DMY ranged between 6.06 t/ha (Adamaoua2) and 13.95 t/ha (cv. Xaraes) for the June cut and between 6.55 t/ha (cv. Adamaoua2) and 10.47 t/ha (cv. Basilisk) for the July cut. For flowering behavior and seed yields, Basilisk flowered earliest while cv. Xaraes flowered latest. In 2020, seed yields were 74.80 kg/ha and 107.72 kg/ha for June cut and July cut regrowth, respectively, whereas in 2021, seed yields were 331 kg/ha and 431 kg/ha for June cut and July cut regrowth, respectively. cv. Adamaoua1 produced the highest seed yield in both years. The cutting regimes evaluated in this study were indifferent for DMY and seed yield.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"70 2","pages":"77-92"},"PeriodicalIF":1.3,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/grs.12420","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139764836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The expression of lignin biosynthesis genes during plant development and effects of downregulated cinnamyl alcohol dehydrogenase (CAD) gene in bahiagrass (Paspalum notatum Flügge) 大叶女贞(Paspalum notatum Flügge)植物发育过程中木质素生物合成基因的表达及肉桂醇脱氢酶(CAD)基因下调的影响
IF 1.3 4区 农林科学
Grassland Science Pub Date : 2024-01-09 DOI: 10.1111/grs.12419
Hwan May Ng, Takahiro Gondo, Tomohito Yamada, Hidenori Tanaka, Ryo Akashi
{"title":"The expression of lignin biosynthesis genes during plant development and effects of downregulated cinnamyl alcohol dehydrogenase (CAD) gene in bahiagrass (Paspalum notatum Flügge)","authors":"Hwan May Ng,&nbsp;Takahiro Gondo,&nbsp;Tomohito Yamada,&nbsp;Hidenori Tanaka,&nbsp;Ryo Akashi","doi":"10.1111/grs.12419","DOIUrl":"10.1111/grs.12419","url":null,"abstract":"<p>Bahiagrass (<i>Paspalum notatum</i> Flügge) is a warm-season grass where high lignin content limits its forage quality, negatively affecting animal performance. To create a new breeding method by genome editing, isolation and characterization of lignin biosynthesis genes and identification of their molecular relationships are essential. The caffeic acid <i>O</i>-methyltransferase (<i>COMT</i>) and cinnamyl alcohol dehydrogenase (<i>CAD</i>) cDNA clones were isolated from bahiagrass, and their protein sequences showed high similarity to other C4 monocot species through phylogenetic analysis. Gene expression analysis of phenylalanine ammonia-lyase (<i>PAL</i>), <i>COMT</i> and <i>CAD</i>, involved in crucial stages of lignin biosynthesis and lignin content, was conducted at different plant developmental stages. The highest gene expression levels of these genes were observed in the vegetative (V) and early reproductive stages, while the lignin content increased until the middle reproductive stage and remained constant thereafter. Additionally, to further understand the molecular relationships in lignin biosynthesis, the effect of <i>CAD</i> downregulation was analyzed in transgenic bahiagrass lines introduced with sorghum <i>CAD</i> antisense and RNAi vector obtained from a previous report. This led to a reduced lignin content and affected the expression of <i>PAL</i> and <i>COMT</i> working upstream of <i>CAD</i>. In the V stage, <i>PAL</i> expression was lower in transgenic lines compared to wild type (WT), while <i>COMT</i> expression showed no significant differences. However, <i>PAL</i> and <i>COMT</i> expression of transgenic lines in the middle reproductive stage (R2) was significantly higher than in the WT. These findings suggest that the downregulation of <i>CAD</i> gene expression affects <i>PAL</i> and <i>COMT</i> expression and induces a feedback system in the R2 stage. Lignin content influenced the phenotype of the transgenic plants with significantly reduced lignin, exhibiting a dwarf phenotype with shorter plant heights. The findings of this study can be applied to genome editing for the development of practical new breeding materials with improved digestibility in bahiagrass.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"70 2","pages":"67-76"},"PeriodicalIF":1.3,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139420740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Methods for prediction of morphophysiological variables of Brachiaria decumbens grass 预测十胜草形态生理变量的方法
IF 1.3 4区 农林科学
Grassland Science Pub Date : 2023-12-18 DOI: 10.1111/grs.12418
Jefte A. A. Conrado, Marcos N. Lopes, Roberto C. F. F. Pompeu, Magno J. D. Cândido
{"title":"Methods for prediction of morphophysiological variables of Brachiaria decumbens grass","authors":"Jefte A. A. Conrado,&nbsp;Marcos N. Lopes,&nbsp;Roberto C. F. F. Pompeu,&nbsp;Magno J. D. Cândido","doi":"10.1111/grs.12418","DOIUrl":"10.1111/grs.12418","url":null,"abstract":"<p>The purpose of this study was to evaluate different methodologies used in the field in order to find the one that could most effectively relate to the morphophysiological condition of <i>Brachiaria decumbens</i> cv. Basilisk (syn. <i>Urochloa decumbens</i> cv. Basilisk Stapf R.D. Webster). Two light interception frequencies (LI, 85% and 95%) and two defoliation intensities were adopted as targets for the residual leaf area index (RLAI 1.3 and 1.8). The treatments consisted of evaluating different methods of measuring the canopy height (CH) through its ability to correlate with the morphophysiological variables of species <i>B. decumbens</i> cv. Basilisk. High correlations between the methods CH, compressed height (CHe), weighted height 1 (WH<sub>1</sub>) and weighted height 8 (WH<sub>8</sub>) were observed mainly with the variables total forage biomass (TFB) and leaf area index (LAI) in both residual and pre-grazing conditions. The path analysis showed degrees of multicollinearity, which determined the use of the “k” constants in order to correct the distortions of the diagonal elements of the correlation matrix X'X. Path analysis provided direct cause/effect indexes on TFB using LAI = 0.1435, photosynthesis (A) = −0.1061, CH = 0.1616, CHe = 0.0913, WH<sub>1</sub> = 0.1386 and WH<sub>8</sub> = 0.0575; on LAI using CH = 0.2404, CHe = 0.2164, WH<sub>1</sub> = 0.2307 and WH<sub>8</sub> = 0.2183; and on photosynthesis rate (A) using LAI = 0.2895, CH = 0.0203, CHe = −0.1170, WH<sub>1</sub> = 0.0352 and WH<sub>8</sub> = 0.0937. Similarity was observed between intercepts and slope coefficients for TFB prediction using CH, CHe, WH<sub>1</sub> and WH<sub>8</sub>, providing mean values of 1069.57 and 204.06, respectively. Methods CH and WH<sub>1</sub> presented a significant correlation with most morphophysiological variables and can be used to quantify TFB and LAI, while CH and WH<sub>8</sub> can be used only for TFB.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"70 2","pages":"51-66"},"PeriodicalIF":1.3,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138825641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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