Grassland Science最新文献

{"title":"Silage fermentation characteristics and bacterial community diversity of sorghum–sudangrass hybrids: A case study on variety selection in Hulunbuir region","authors":"Zhaorui Han,&nbsp;Xingzhao Zhou,&nbsp;Chao Jiang,&nbsp;Wenge Li,&nbsp;Xun Chen,&nbsp;Weiqiang Song,&nbsp;Xiwen Su,&nbsp;Siran Wang,&nbsp;Yanzi Xiao","doi":"10.1111/grs.70043","DOIUrl":"https://doi.org/10.1111/grs.70043","url":null,"abstract":"<p>Selecting high-quality forage cultivars is essential for stabilizing regional forage supply and enhancing feed conversion efficiency. To identify suitable varieties for the Hulunbuir region, this study evaluated five sorghum–sudangrass hybrids (designated SS1–SS5). The evaluation was conducted by comprehensively analyzing their agronomic traits, silage quality and bacterial community diversity. Forage materials were harvested at the late milk stage. After being chopped and vacuum-sealed, the samples were subjected to 60-day fermentation. Among the tested hybrids, SS4 exhibited superior comprehensive performance. It achieved the highest (<i>P</i> &lt; .05) fresh forage yield (8.48 t/hm²) and the largest (<i>P</i> &lt; .05) stem diameter (22.00 mm). In terms of silage fermentation quality, SS4 also outperformed its counterparts. It was characterized by the highest (<i>P</i> &lt; .05) lactic acid concentration (26.30 g/kg DM), which was markedly higher (<i>P</i> &lt; .05) than the range of 14.20–19.50 g/kg DM observed in other hybrids. Concurrently, SS4 had the lower (<i>P</i> &lt; .05) pH value (3.67 vs. 3.73–3.95). It also showed lower neutral detergent fiber content (540 g/kg DM vs. 603–640 g/kg DM) and acid detergent fiber content (317 g/kg DM vs. 388–430 g/kg DM). Microbiological analysis showed that ensiling shifted bacterial dominance from Proteobacteria to Firmicutes. Specifically, SS4 selectively enriched <i>Lactobacillu</i>s (58.2%) and <i>Nitrospira</i> (2.5%). The Kyoto Encyclopedia of Genes and Genomes (KEGG) functional prediction further indicated that this bacterial community succession substantially upregulated carbohydrate and energy metabolism pathways. Furthermore, SS4 presented a robust fibrolytic potential, as evidenced by the enhanced (<i>P</i> &lt; .05) expression of cellulase (EC 3.2.1.4) and endo-1,4-<i>β</i>-xylanase (EC 3.2.1.8). In conclusion, SS4 is the optimal candidate for promotion as the primary forage variety in the Hulunbuir region.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"72 4","pages":"281-295"},"PeriodicalIF":1.2,"publicationDate":"2026-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149099578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term forage yield and botanical composition dynamics in an andosol grassland under 54 years of fertilizer treatments","authors":"Takehiko Matsumoto,&nbsp;Toshinobu Koba,&nbsp;Toshiya Saigusa","doi":"10.1111/grs.70040","DOIUrl":"https://doi.org/10.1111/grs.70040","url":null,"abstract":"<p>Herein, a long-term fertilizer application trial was conducted on a clover (CL)–grass mixed sown grassland established on the andosols in Hokkaido's Konsen district. The experiment combined the presence or absence of the three main nutrients—nitrogen (N), phosphorus (P) and potassium (K)—with or without calcium (Ca) and magnesium (Mg) fertilization. We assessed the influence of fertilization on forage yield and alterations in botanical composition up to the 54th year following establishment. The treatments that maintained high yield levels for &gt;50 years were the no nitrogen (−N) and three-nutrients (3F) plots with Ca and Mg applications. These findings support the statement by Omura et al. (1985) that stable high-yield forage production requires maintaining sown species in the Konsen region, especially CL, restraining N fertilization, applying sufficient P and K, and maintaining an appropriate soil pH through Ca and Mg fertilization. Contrastingly, the continuation of the trial clearly showed its high adaptability to low soil pH, although the redtop (RT, <i>Agrostis alba</i> L.) had previously been regarded only as tolerant to low-P conditions.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"72 4","pages":"257-265"},"PeriodicalIF":1.2,"publicationDate":"2026-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149100744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Empirical prediction of organic matter digestibility in grasses, legumes and their mixtures from dairy farms in Atlantic Spain","authors":"Sonia Pereira Crespo,&nbsp;Pilar Gago,&nbsp;Adrián Botana,&nbsp;Marcos Veiga,&nbsp;Laura González,&nbsp;Juan Valladares,&nbsp;Roberto Lorenzana,&nbsp;César Resch,&nbsp;María del Pilar Martínez-Diz,&nbsp;Dalia Andrea Plata-Reyes,&nbsp;Gonzalo Flores-Calvete","doi":"10.1111/grs.70042","DOIUrl":"https://doi.org/10.1111/grs.70042","url":null,"abstract":"<p>This study characterized the chemical composition and organic matter digestibility (OMD) of herbage from commercial dairy farms in Galicia (NW Spain), a humid-temperate Atlantic region, and developed empirical prediction equations for OMD based on routine wet-chemistry traits. A total of 593 samples were collected over 7 years (2007–2013), including grasses (<i>n</i> = 235; e.g., Italian and perennial ryegrass, cocksfoot, fescue), legumes (<i>n</i> = 212; e.g., white/red/crimson/Persian clovers, serradella, alfalfa) and grass–legume mixtures (<i>n</i> = 146). OMD was determined by a two-stage in vitro method (buffered rumen fluid and pepsin).</p><p>Across the full dataset, acid detergent fiber (ADF) and neutral detergent fiber (NDF) were the strongest predictors of OMD. The best-performing overall equation was: OMD (%) = 106.30–0.82·ADF − 0.25·CP − 0.37·HCEL, achieving external validation <i>r</i>² = 0.69, standard error of prediction (SEP) = ±3.66%, ratio of performance to deviation (RPDev) = 1.80 and range error ratio (RERev) = 9.1. Group-specific equations were as follows:</p><p>Grasses: OMD (%) = 79.72–0.55·NDF − 0.21·OM (external validation <i>r</i>² = 0.54; SEP = ±3.60%; RPDev ≈ 1.39–1.47; RERev ≈ 8.0–8.4);</p><p>Legumes: OMD (%) = 75.67–0.51·NDF + 0.28·CP + 1.66·WSC − 0.047·WSC² (external validation <i>r</i>² = 0.75; SEP = ±3.43%);</p><p>Mixtures: OMD (%) = 88.12–0.70·ADF + 0.25·WSC (external validation <i>r</i>² = 0.43; SEP = ±3.93%; RPDev ≈ 1.16–1.32; RERev ≈ 6.0–6.8).</p><p>These results show that empirical equations based on routinely available chemical analyses can provide a rapid, cost-effective tool to estimate herbage OMD for Atlantic dairy systems; however, transfer to contrasting climates should be preceded by local validation or recalibration.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"72 4","pages":"266-280"},"PeriodicalIF":1.2,"publicationDate":"2026-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/grs.70042","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149099437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shorter cutting length slightly enhances nutrient retention and fermentation efficiency of heavily wilted Italian ryegrass silage","authors":"Li Zhuang Wu,&nbsp;Yan Fen Li,&nbsp;Xaysana. Panyavong,&nbsp;Eun Chan Jeong,&nbsp;Kun Jun Han,&nbsp;Jong Geun Kim","doi":"10.1111/grs.70038","DOIUrl":"https://doi.org/10.1111/grs.70038","url":null,"abstract":"<p>This study evaluated the effects of different theoretical lengths of cut on nutrient preservation and fermentation kinetics of low-moisture Italian ryegrass (approximately 80% dry matter [DM]). After harvest, the forage was field-wilted for 2 days and then manually chopped to 10, 20, or 30 mm for ensiling, and silage samples were collected on Days 1, 2, 3, 5, 10, 20, 30, 45, and 60. During fermentation, DM content initially decreased and subsequently increased, resulting in a slight increase for the 10-mm group but a significant decrease for the 30-mm group (from 84.06% to 78.88%, <i>p</i> &lt; .05). Structural carbohydrate concentrations increased after ensiling, with both neutral detergent fiber (NDF) and acid detergent fiber (ADF) showing significant elevations across treatments. The most pronounced increase occurred in the 30-mm group, particularly for NDF (from 69.45% to 75.71%, <i>p</i> &lt; .01). CP content in the 20-mm group was significantly lower than that in the 10- and 30-mm groups (<i>p</i> &lt; .01), whereas in vitro dry matter (DM) digestibility remained relatively stable and did not differ among treatments. Fermentation patterns were similar across groups, as pH and water-soluble carbohydrate (WSC) contents declined only slightly throughout ensiling. However, during the early fermentation stage (first 10 days), the 10-mm group exhibited a relatively rapid decline in pH, reaching 6.16. Accordingly, lactic acid accumulation was significantly higher in the 10- and 20-mm groups (0.84% and 1.40% DM, respectively) than in the 30-mm group (0.46% DM, <i>p</i> &lt; .01). By the end of ensiling, the 10-mm treatment achieved the highest TDN level. Overall, shorter theoretical lengths of cut promoted early lactic acid fermentation and more effective acidification in heavily wilted forage. Therefore, reducing cutting length to approximately 10 mm is recommended to improve the fermentation quality of low-moisture Italian ryegrass silage.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"72 4","pages":"233-243"},"PeriodicalIF":1.2,"publicationDate":"2026-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149100381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactiplantibacillus plantarum and cellulase synergistically improve the fermentation quality, chemical compositions, and bacterial community structure of Leymus chinensis silage","authors":"Tianrong Zhou,&nbsp;Yichao Liu,&nbsp;Pengbo Sun,&nbsp;Mingjiu Wang,&nbsp;Wei Wang,&nbsp;Minglu Zuo,&nbsp;Siran Wang,&nbsp;Ge Gentu","doi":"10.1111/grs.70039","DOIUrl":"https://doi.org/10.1111/grs.70039","url":null,"abstract":"<p><i>Leymus chinensis</i> (Trin.) Tzvel is a high-quality perennial native grass species in China, boasting a host of desirable traits such as high biomass yield, superior forage quality and excellent palatability for livestock. The purpose of this study was to explore <i>Lactiplantibacillus plantarum</i> (LP), 5 × 10⁶ CFU/g of fresh weight (FW); cellulase (CE, xylanase and cellulase activities of 100,000 and 10,000 μ/g, 1% FW), and their combinations (LPCE, 5 × 10⁶ CFU/g FW). Silage fermentation quality and bacterial community of <i>L. chinensis</i> were analyzed after 60 days of fermentation. The chopped material (500 g) was mixed well and then packaged into individual polyethylene bags (dimensions 22 cm × 35 cm) with three replications. Following a 60-day ensiling period, all additives significantly decreased the pH compared with the control group (CK, <i>p</i> &lt; .05). The lactic acid (LA) and water-soluble carbohydrate (WSC) contents accumulated (<i>p</i> &lt; .05), whereas acidic detergent fiber (ADF) and neutral detergent fiber (NDF) contents decreased (<i>p</i> &lt; .05) in LPCE group on Day 60, and LPCE group exhibited superior fermentation quality, as indicated by large amounts of LA (~18.75 g/kg dry matter [DM]), relatively low pH (~4.01), acceptable levels of ammonia nitrogen (~5.38 g/kg total nitrogen) and trace amounts of butyric acid (&lt;2 g/kg DM). <i>Lactiplantibacillus</i> was dominant in all treated groups, whereas <i>Enterobacter</i> was predominant in CK. The LP and LPCE groups inhibited the reproduction threats posed by harmful microorganisms and decreased the growth and reproduction of <i>Enterobacter</i>, <i>Pseudomonas</i> and <i>Pantoea</i> during ensiling. Overall, LPCE can be used as a strategy to improve the fermentation quality, chemical composition and microbial community composition of <i>Leymus chinensis</i> silage.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"72 4","pages":"244-256"},"PeriodicalIF":1.2,"publicationDate":"2026-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149100745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in viability between two Epichloë endophyte species (E. uncinata and E. occultans) in Italian ryegrass (Lolium multiflorum Lam.) seeds during storage","authors":"Ken-ichi Tamura,&nbsp;Nobuyo Yoshida,&nbsp;Takako Kiyoshi,&nbsp;Koya Sugawara","doi":"10.1111/grs.70044","DOIUrl":"https://doi.org/10.1111/grs.70044","url":null,"abstract":"<p><i>Epichloë uncinata</i> (<i>Eu</i>), which has a symbiotic relationship with meadow fescue in nature, can be cultured and artificially inoculated, raising expectations for its expanded use in the breeding of other grass species. However, its introduction into non-native host species may increase the risk of unstable symbiosis. In this study, we aimed to compare the stability of <i>Eu</i> and <i>E. occultans</i> (<i>Eo</i>), a species naturally associated with Italian ryegrass (IR; <i>Lolium multiflorum</i> Lam.), in IR seeds. Endophyte viability tests under seed storage with accelerated aging conditions using genetically similar IR seeds revealed a significantly lower stability of the <i>Eu</i> Eto8 strain than that of the <i>Eo</i> strain. This suggests that the risk of reduced endophyte infection rates in the <i>Eu</i>–IR symbiotic system depends on the seed storage conditions. Factors other than endophyte density or localization may have contributed to the differences in viability between the <i>Epichloë</i> strains in the IR seeds.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"72 4","pages":"296-302"},"PeriodicalIF":1.2,"publicationDate":"2026-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149099751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing soil and native high Andean grassland quality under grazing: A case study from the wet Puna of Peru","authors":"Alberto Arias-Arredondo,&nbsp;Wendy E. Pérez,&nbsp;Sphyros Lastra,&nbsp;Juancarlos Cruz,&nbsp;Richard Solórzano-Acosta,&nbsp;Cecilia Turín","doi":"10.1111/grs.70029","DOIUrl":"https://doi.org/10.1111/grs.70029","url":null,"abstract":"<p>High Andean grasslands are vulnerable to changes in their nutritional quality and carbon sequestration capacity, especially in grazing systems. This study evaluated soil quality and native grasses by measuring carbon, physicochemical parameters, and the nutritional quality of predominant species in the wet Puna of Junín, Peru. Significant differences were found in carbon storage and nutritional quality across different grazing grassland sites. Soil carbon levels were consistently high across all sites, with significant concentrations at San Pedro de Cajas (14.26% ± 11.7%; <i>p</i> &lt; 0.05), and its carbon stock (210.7 ± 111.3 Mg/ha) exceeded that of Junín (+68%) and Ulcumayo (+107%). Also, the flat topography at this site is presumed to have influenced its soil fertility. No adverse relationship was observed between carbon content and animal units, regardless of continuous or rotational grazing system, and a strong negative Pearson correlation (<i>r</i> = −0.84) between total carbon and bulk density indicated the prevalence of organic materials and no soil compaction. It emphasizes how landscape features affect soil quality and ecosystem processes. The protein content of key species exhibited a strong Pearson correlation with plant phosphorus (<i>r</i> = 0.93), digestible organic matter (DOM), and metabolizable energy (ME; <i>r</i> = 0.75). The Redundandy Analysis (RDA) showed that plant functional traits are driven by chemical and physical gradients, underscoring the combined effects of nutrient availability and site constraints on pasture productivity and forage quality. Among native grasses, <i>Cebadilla</i> (<i>Calamagrostis vicunarum</i> [Wedd.] Pilg.) emerged as the most favorable option for animal nutrition and exhibited a significantly higher crude protein content (8.23% ± 0.89%), Metabolizable energy value (8.16 MJ/kg), and C/N ratio (~40). Future research should focus on linking soil and forage quality with animal physiological responses to improve understanding of grazing impacts and guide sustainable management in high-altitude grasslands.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"72 3","pages":"163-174"},"PeriodicalIF":1.2,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/grs.70029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148373329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calcium deficiency symptoms in forage corn (Zea mays L.) due to excessive potassium supply under field conditions: Using leaf serration as a soil potassium indicator","authors":"Yoshihito Sunaga,&nbsp;Hisatomi Harada,&nbsp;Tahei Kawachi","doi":"10.1111/grs.70030","DOIUrl":"https://doi.org/10.1111/grs.70030","url":null,"abstract":"<p>Leaf serration in forage corn (<i>Zea mays</i> L.) is often observed under field conditions. In this study, field cultivation experiments were conducted over 3 years to determine the factors affecting the appearance and development of this trait and to assess its potential impact on corn productivity. We also evaluated soil mineral conditions using leaf serration as an indicator. The number of symptomatic leaves per plant decreased after spraying with calcium chloride solution (3 g/L), and the development of leaf serration was also suppressed. Therefore, leaf serration in corn grown under field conditions may be a symptom of calcium deficiency. Occurrence of leaf serration did not significantly affect the dry matter yield of corn shoots at the yellow-ripe stage, which is considered optimal for ensiling. This was because the yield, which included the leaves, stems, and ears, was not affected by calcium spraying. The number of leaves with symptoms increased logarithmically with an increase in soil exchangeable potassium content, owing to the decrease in calcium concentration in the corn plant caused by excessive amounts of soil potassium. The appearance of symptoms differed significantly among corn varieties, and the highest number of symptomatic leaves occurred in the variety with the highest calcium concentration. Different regression equations were obtained from the relationships between soil exchangeable potassium content and the number of symptomatic leaves in varieties with low and high calcium concentrations. The soil exchangeable potassium content was estimated using these equations, with the number of symptomatic corn leaves after silking as an indicator. With two or more symptomatic leaves per plant, the soil exchangeable potassium content was adequate for forage corn production. We suggest that a reduction in potassium input should be implemented in fertilization management if corn plants develop leaf serration.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"72 3","pages":"175-187"},"PeriodicalIF":1.2,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148373352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning for predicting dry matter yields of the first harvest following overwintering in perennial ryegrass (Lolium perrene L.)","authors":"Tsuneki Tanaka,&nbsp;Yuki Akiyama,&nbsp;Yoshiki Sumiya,&nbsp;Hiroki Fujii,&nbsp;Toshihiko Yamada","doi":"10.1111/grs.70031","DOIUrl":"https://doi.org/10.1111/grs.70031","url":null,"abstract":"<p>Predicting the dry matter yield (DMY) of the first harvest of perennial ryegrass (<i>Lolium perenne</i> L.) following overwintering helps assess the risk of winter damage. This study compared machine learning (ML) models for the prediction of DMYs based on well-organized examined data for cultivar registration trials across 13 locations in Hokkaido, Japan, over the period from 1983 to 2022, including the DMYs of first harvests, daily weather data, seedling and examined years, number of harvests in the previous year, date of last harvest in the autumn of the previous year, date of first harvest and experimental designs (drilled-row plots or sward plots, and whether mixed with other species). The yield estimation models were developed using 17 ML methods with default hyperparameter settings. Among the ML models, successful methods included tree-based algorithms. The accuracy scores (<i>R</i>^<i>2</i>) were 0.80–0.91 for training data (5-fold cross-validation), and 0.79–0.92 for test data. The permutation feature importance in the best model (light gradient boosting machine) indicated that the daily minimum temperature and snow depth in winter could have a significant impact on the DMYs of the first harvests following overwintering. Additionally, the partial dependence plots revealed that frequent cutting increased the risk of overwintering damage and that it identified a specific autumn harvest period that negatively affected yield and persistence after overwintering. In conclusion, the ML model, incorporating weather and pasture management data, demonstrated high predictive performance (<i>R</i>^<i>2</i> = 0.92) for forecasting overwintered yield loss of perennial ryegrass in frozen soil areas of Hokkaido.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"72 3","pages":"188-198"},"PeriodicalIF":1.2,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148373309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Papaya peel enhances fermentation quality and nutritional value of mixed corn straw-rice straw silage","authors":"Jingzhen Li,&nbsp;Li Liang,&nbsp;Huade Xie,&nbsp;Fanquan Zeng,&nbsp;Xianqing Luo,&nbsp;Chengjian Yang","doi":"10.1111/grs.70033","DOIUrl":"https://doi.org/10.1111/grs.70033","url":null,"abstract":"<p>This study investigated the effects of papaya peel incorporation (0%, 15% and 30%) into a 50:50 mixture of corn and rice straw on fermentation quality, chemical composition and in vitro ruminal digestion. The silage was prepared 1 kg in a fermentation bag (16 cm × 25 cm) and ensiled for 45 days at room temperature (15–25 °C), with six replicates per treatment. The results showed that papaya peel addition significantly (<i>P</i> &lt; 0.05) decreased the dry matter and neutral detergent fiber contents of mixed silage, while significantly (<i>P</i> &lt; 0.05) increasing the crude protein and water-soluble carbohydrate contents of mixed silage. Compared to the control group, both treatments (15% or 30% papaya peel) exhibited higher acetic acid and ammonia-N concentrations (<i>P</i> &lt; 0.05). The 30% papaya peel treatment additionally showed higher lactic acid bacteria counts and lower yeast populations (<i>P</i> &lt; 0.05). In vitro fermentation results showed that 30% papaya peel treatment achieved the highest in vitro dry matter digestibility (<i>P</i> &lt; 0.05). This study demonstrates that incorporating 30% papaya peel effectively improves the quality of mixed corn straw and rice straw silage, enhancing its nutritional value for ruminants.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"72 3","pages":"199-207"},"PeriodicalIF":1.2,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148373624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}