Journal of Herbal Medicine最新文献

{"title":"Therapeutic potential of wild rare plant-derived herbal ointment: a promising intervention for managing chronic dermatological conditions","authors":"Mohamad Al Dhareef Al Shamsi","doi":"10.1016/j.hermed.2024.100920","DOIUrl":"10.1016/j.hermed.2024.100920","url":null,"abstract":"<div><h3>Introduction</h3><p>Chronic skin conditions have become a significant global healthcare issue, necessitating the development of novel therapeutic approaches. This study investigates the medicinal properties of ABBA Herbal Ointment, derived from the wild plant <em>Cymbopogon schoenanthus</em> ssp. <em>proximus.</em> Introduced in 2001, this ointment contains natural ingredients such as Aloe vera, jojoba oil, and calendula, extracted from desert medicinal plants. It has demonstrated swift and conclusive efficacy in treating chronic skin conditions. Clinically verified and patented in Gulf Cooperation Council (GCC) countries (Patent Number: GC 0001218), its therapeutic potential is supported by extensive research conducted by three French laboratories.</p></div><div><h3>Methods</h3><p>The traditional method is used to produce the ointment from the plant extract. To make the ointment, the extracted plant chlorophyll was homogenised with olive oil and natural beeswax. To further validate the therapeutic potential of the ointment, in vitro occlusive tests were performed to assess its efficacy on human skin, with results observed after 48 hours. In this study, 24 volunteers participated, of which two discontinued.</p></div><div><h3>Results</h3><p>The topical application of the ointment shows no side effects, and further trials demonstrate its beneficial effects in managing chronic skin diseases.</p></div><div><h3>Conclusion</h3><p>ABBA Herbal Ointment, derived from the rare wild plant <em>C. schoenanthus</em> ssp. <em>proximus,</em> shows significant potential as an effective treatment for chronic dermatological conditions. The study supports the ointment's efficacy and safety, aligning with the goal of developing novel therapeutic approaches for chronic skin diseases.</p></div>","PeriodicalId":56077,"journal":{"name":"Journal of Herbal Medicine","volume":"47 ","pages":"Article 100920"},"PeriodicalIF":2.2,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141853569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The inheritance and development of traditional Chinese medicine formulas in treating childhood asthma based on the perspective of clinical research","authors":"Fangyang Guo ,&nbsp;Yonghuan Xiao ,&nbsp;Chaitanya N. Makwana ,&nbsp;Yuan He ,&nbsp;Pan Luo ,&nbsp;Muhammad Irfan ,&nbsp;Zhiwen Xiao ,&nbsp;Ling Li ,&nbsp;Meiying Ao ,&nbsp;Qian Liu","doi":"10.1016/j.hermed.2024.100921","DOIUrl":"10.1016/j.hermed.2024.100921","url":null,"abstract":"<div><h3>Introduction</h3><p>The inheritance and development status are illustrated with research hotspots, medication patterns, and research quality based on the two literature databases of previous and recent clinical investigations of Traditional Chinese Medicine formulas in treating childhood asthma.</p></div><div><h3>Methods</h3><p>The relevant literature was retrieved from seven databases. Based on the year of publication, dividing the body of literature produced since the database's founding into previous and recent databases. Bibliometric, VOSviewer, IBM SPSS Modeller and modified Jadad scale were used to conduct characteristic analysis, visual analysis, herb compatibility rule analysis and research quality evaluation respectively.</p></div><div><h3>Results</h3><p>One hundred and seven previous studies and 212 recent studies were included. The outcome indicators are inherited symptoms and syndrome, and develop into mechanistic indicators such as pulmonary function. The common herbs are <em>Glycyrrhiza uralensis</em> Fisch., <em>Ephedra sinica</em> Stapf and <em>Prunus armeniaca L.var.ansu</em> Maxim., and the development of <em>Schisandra chinensis</em> (Turcz.) Baill., <em>Astragalus membranaceus</em> (Fisch.) Bge.var. <em>mongholicus</em> (Bge.) Hsiao and <em>Poria cocos</em> (Schw.) Wolf are increasing. The compatibility of herbs inherited the combination of <em>E. sinica</em> Stapf and <em>G. uralensis</em> Fisch. based on the development of <em>Asarum heterotropoides</em> Fr. <em>Schmidt var. mandshuricum</em> (Maxim.) Kitag., <em>S. chinensis</em> (Turcz.) Baill. and <em>Pinellia ternata</em> (Thunb.) Breit. The research quality has been improved.</p></div><div><h3>Conclusion</h3><p>From the clinical research perspective, the study of Traditional Chinese Medicine formulas for the treatment of childhood asthma has its own unique inheritace and development, covering a number of areas.</p></div>","PeriodicalId":56077,"journal":{"name":"Journal of Herbal Medicine","volume":"47 ","pages":"Article 100921"},"PeriodicalIF":2.2,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141846172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estrogenic analysis of ten Thai medicinal plants used for treating menstrual irregularity by ERα and ERβ yeast two-hybrid systems","authors":"Palita Paewthaisong,&nbsp;Ratima Janthima,&nbsp;Sineenat Siri","doi":"10.1016/j.hermed.2024.100922","DOIUrl":"10.1016/j.hermed.2024.100922","url":null,"abstract":"<div><h3>Introduction</h3><p>Several traditional Thai medicinal plants have historically been utilised to alleviate menstrual irregularities. While it is hypothesised that phytoestrogens are pivotal in their medicinal efficacy, more empirical evidence is needed to verify this assumption. Furthermore, our understanding of the estrogenic effects mediated by estrogen receptors (ERα and ERβ) and their alterations post-intestinal enzymatic digestion is limited. This study aims to assess the estrogenic activity of 10 medicinal plants before and after <em>in vitro</em> simulated gastrointestinal digestion using yeast two-hybrid (Y2H) systems.</p></div><div><h3>Methods</h3><p>Y2H systems were constructed based on the interaction between the ligand-binding domain of estrogen receptors and the receptor interaction domain of coactivators. The medicinal plants were extracted in water at 95 °C for 1 hour or in 70% ethanol for 24 hours (1: 8 w/v). Their estrogenic activities before and after <em>in vitro</em> gastrointestinal digestion were determined using Y2H systems.</p></div><div><h3>Results</h3><p>The majority of ethanol extracts exhibited higher estrogenic activity compared to water extracts, with detection notably facilitated by the ERβ-Y2H system. This study represents the first documentation of the estrogenic activities of <em>Dendrolobium lanceolatum</em> and <em>Morinda coreia</em>, both prominently identified by the ERβ-Y2H system. Specifically, the <em>D. lanceolatum</em> extract demonstrated the highest estrogenic activity, showing a significant enhancement following gastrointestinal enzymatic digestion, suggesting the presence of active metabolites.</p></div><div><h3>Conclusions</h3><p>These findings advance our understanding of the estrogenic properties of medicinal plants for treating menstrual irregularities, clarifying their effects on estrogen receptor subtypes and post-hydrolysis activities. Such insights are vital for determining their potential as alternative medicines.</p></div>","PeriodicalId":56077,"journal":{"name":"Journal of Herbal Medicine","volume":"47 ","pages":"Article 100922"},"PeriodicalIF":2.2,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141849201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of herbal medicine for hypertension in rural and urban Thailand: a cross sectional study","authors":"Nutchar Wiwatkunupakarn ,&nbsp;Chanchanok Aramrat ,&nbsp;Pimtip Sanguanwai ,&nbsp;Yanee Choksomngam ,&nbsp;Mary E. Gilder ,&nbsp;Wichuda Jiraporncharoen ,&nbsp;Rose McGready ,&nbsp;Chaisiri Angkurawaranon","doi":"10.1016/j.hermed.2024.100916","DOIUrl":"10.1016/j.hermed.2024.100916","url":null,"abstract":"<div><h3>Introduction</h3><p>Using herbal medicine to treat hypertension is common in Thailand. However, there is variation in the use of herbs between rural and urban areas. This study aims to observe the prevalence of herb use, describe herb users’ behaviours, and describe anti-hypertensive drug compliance among the users.</p></div><div><h3>Methods</h3><p>A cross-sectional survey among hypertensive patients visiting primary care services in Tak (rural) and Chiang Mai (urban) provinces was conducted between March and April 2019. Patients were assessed by using questionnaires that gathered information about patient demographic data, history of hypertension, herb use behaviours, and the concurrent use of standard treatment.</p></div><div><h3>Results</h3><p>In total, 400 participants were included in this study—200 from rural and 200 from urban areas. Sixty-two percent were female. The prevalence of herb use was 13.0% in urban areas and 17.5% in the rural areas. Rural participants reported a significantly longer duration of herb use (<em>P</em> &lt; 0.01), and a significantly higher proportion of using unapproved herbal products (91% vs 54%, <em>P</em> &lt; 0.001). Participants mostly use herbs along with standard treatment (86.9%); nevertheless, poor drug compliance was similar in both areas, with rural participants more frequently taking their medications at the wrong time (<em>P</em> = 0.03).</p></div><div><h3>Conclusion</h3><p>There was a trend of higher use of unapproved herbals in rural participants. Drug compliance among herb users was poor. It is recommended that a comprehensive assessment of herb use is conducted during care for hypertensive patients in this and similar contexts.</p></div>","PeriodicalId":56077,"journal":{"name":"Journal of Herbal Medicine","volume":"47 ","pages":"Article 100916"},"PeriodicalIF":2.2,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2210803324000733/pdfft?md5=367ee37ac69e353ba391bfa4c323bcd9&pid=1-s2.0-S2210803324000733-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141849487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on ethnomedicine, phytochemistry, pharmacology, and toxicology of Pedalium murex L.","authors":"Amrender Singh Rao,&nbsp;Surender Singh Yadav,&nbsp;Neetu Singh,&nbsp;Abhishek Nandal","doi":"10.1016/j.hermed.2024.100917","DOIUrl":"10.1016/j.hermed.2024.100917","url":null,"abstract":"<div><h3>Introduction</h3><p><em>Pedalium murex</em> L. (Gokhru) is a succulent herbaceous plant with multifarious medicinal values in various traditional therapeutic systems. The review summarises the previous research findings on <em>P. murex</em>, with major emphasis on ethnomedicine, phytochemistry, pharmacology, and toxicology.</p></div><div><h3>Material and methods</h3><p>The study was performed by retrieving relevant literature from scientific databases like Scopus, Science Direct, Springer Link, Wiley, PubMed, Google Scholar, Elsevier, and Web of Science. The data collection was carried out using specific keywords like ‘<em>P. murex</em>,’ ‘Gokhru,’ ‘ethnomedicinal uses,’ ‘Phytochemistry<em>,’ ‘</em>Pharmacology<em>,’ ‘</em>Toxicity,’ ‘Clinical studies’ and their combinations.</p></div><div><h3>Results</h3><p>The study's findings revealed a strong connection between <em>P. murex</em> traditional use and pharmacological activities. The plant shows aphrodisiac, antiulcer, anti-inflammatory, antioxidant, antimicrobial, antidiabetic, antivenereal, anticancer, analgesic, antipyretic, immune-modulator, and other pharmacological properties. Phytocompounds like diosgenin, pedalin, pedalitin, <em>β</em>-sitosterol, isatin, lupeol acetate, and ursolic acid are amongst the many which are responsible for various therapeutic applications and have the potential to transform into oral drugs.The plant shows immune-modulatory effects by influencing corticosterone, low-density lipoprotein, thiobarbituric acid, lipid peroxidation, nitric oxide, exo-polysaccharides, Cyclooxygenase and free radical inhibitory mechanisms for neuroprotection, hypolipidemic, antidiabetic, antibiofilm, aphrodisiac, and anti-inflammatory activities respectively. Toxicological studies implied safety and protection indicating potential to be used as herbal medicine.</p></div><div><h3>Conclusion</h3><p>The different parts of the plant have potential bioactive properties with prospective ethnopharmacological relevance for aphrodisiac, venereal, urinary tract infections and inflammation. Further, it is necessary to conduct controlled clinical trials, in-depth pharmacokinetic, detailed phytochemical and pharmacological studies to achieve the plant’s full potential.</p></div>","PeriodicalId":56077,"journal":{"name":"Journal of Herbal Medicine","volume":"47 ","pages":"Article 100917"},"PeriodicalIF":2.2,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141853059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimisation and Evaluation of the Correlation of Extraction Conditions for Total Flavonoid Content and Antixanthine Oxidase Activity in Psidium guajava","authors":"T.M. Le ,&nbsp;C.D.P. Nguyen ,&nbsp;A.C. Ha","doi":"10.1016/j.hermed.2024.100915","DOIUrl":"10.1016/j.hermed.2024.100915","url":null,"abstract":"<div><h3>Introduction</h3><p><em>Psidium guajava</em> L. (<em>P guajava</em>) and its leaf, one of the most popular plants in traditional medicine and cuisine, were demonstrated to have high flavonoid content with many bioactivities, including antigout activity. However, the correlation between total flavonoid content (<strong>TFC</strong>) and antigout activities and the optimal conditions for extracting <em>P guajava</em> have not been reported.</p></div><div><h3>Methods</h3><p>This study established the optimisation of ethanol extracts of <em>P guajava</em> leaves for TFC and antigout activities using the single-factor method and response surface methodology. The antigout activities were determined through the xanthine oxidase inhibition (<strong>XOI</strong>) method.</p></div><div><h3>Results</h3><p>The four independent variables (ethanol concentration, plant-solvent ratio, extraction time, and temperature) significantly impacted two response variables (TFC and XOI activity). The optimal extracts for XOI activity reach the maximum TFC of 323.86 ± 2.26 mg quercetin/g and XOI activity of 26.37 ± 0.5 μg/ml. The changes in the chemical structure of extracts were analysed by Fourier transform infrared, which indicated that several flavonoids in <em>P guajava</em> do not have XOI activity. The correlation of TFC and XOI is robust with the Pearson correlation of −0.882 and Spearman Rho test of −0.884. Additionally, the biological activities, such as antioxidant and anti-inflammatory, of optimal extracts for XOI and TFC were determined to extend the application capacity to support the treatment of gout diseases.</p></div><div><h3>Conclusions</h3><p>The optimised extraction conditions are critical to isolating flavonoids, which can serve as functional food or medicine for managing gout and its complications.</p></div>","PeriodicalId":56077,"journal":{"name":"Journal of Herbal Medicine","volume":"47 ","pages":"Article 100915"},"PeriodicalIF":2.2,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141709666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Effect of Lantana camara ethanolic leaf extract on survival and migration of MDA-MB-231 triple-negative breast cancer cell line” [Journal of Herbal Medicine 43 (2024) 100837]","authors":"Arundhaty Pal,&nbsp;Sourav Sanyal,&nbsp;Sayantani Das,&nbsp;Tapas K. Sengupta","doi":"10.1016/j.hermed.2024.100910","DOIUrl":"https://doi.org/10.1016/j.hermed.2024.100910","url":null,"abstract":"","PeriodicalId":56077,"journal":{"name":"Journal of Herbal Medicine","volume":"46 ","pages":"Article 100910"},"PeriodicalIF":2.2,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2210803324000678/pdfft?md5=806a233574531b702d26a5488bf48143&pid=1-s2.0-S2210803324000678-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141605605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can herbal teas be used as complementary therapies for kidney disorders? Results from a cross-sectional study","authors":"Firouzeh Moeinzadeh ,&nbsp;Mohammad Hossein Rouhani ,&nbsp;Mojgan Mortazavi ,&nbsp;Farnaz Shahdadian","doi":"10.1016/j.hermed.2024.100914","DOIUrl":"10.1016/j.hermed.2024.100914","url":null,"abstract":"<div><h3>Introduction</h3><p>Chronic kidney disease (CKD) has limited treatment options and is becoming more and more common. Since apparent evidence in the literature shows that herbs may be reno-protective, we discussed different kinds of herbal tea in relation to CKD and related disorders.</p></div><div><h3>Methods</h3><p>This cross-sectional study was conducted in health care centres located in a region in central Iran by enroling subjects aged 18 years and older. A self-reported questionnaire was used to evaluate the frequency and type of herbal tea drinks by participants. Anthropometric characteristics were measured and blood samples were obtained using a standard procedure. The Kidney Disease Outcome Quality Initiative (K/DOQI) guideline was the reference for diagnosis of CKD. Through multiple logistic models, the association between herbal tea drinking with CKD and related disorders was examined.</p></div><div><h3>Results</h3><p>Overall, 3 322 subjects were examined in this study. Three types of herbal tea were consumed by the study participants including green tea, chamomile-thyme tea, and borage tea. No statistically significant consistent associations between kidney disorders, including CKD, haematuria, albuminuria, and kidney stone and consumption of these herbal preparations were identified.</p></div><div><h3>Conclusion</h3><p>Overall, this study failed to suggest any consistent association between renal function and three categories of herbal tea in adult Iranian individuals.</p></div>","PeriodicalId":56077,"journal":{"name":"Journal of Herbal Medicine","volume":"46 ","pages":"Article 100914"},"PeriodicalIF":2.2,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141715924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Study on the Effects of Myrtle and Chamomile Essential Oils on Pain and Episiotomy Wound Healing in Primiparous Women: A Randomised Controlled Clinical","authors":"S. Moradkhani ,&nbsp;S. Abdoli ,&nbsp;E.T. Ghane ,&nbsp;E. Jenabi","doi":"10.1016/j.hermed.2024.100913","DOIUrl":"10.1016/j.hermed.2024.100913","url":null,"abstract":"<div><h3>Introduction</h3><p>Episiotomy is a common surgical procedure that affects the quality of life of women. Chamomile and myrtle have been used to treat wounds since antiquity. This study aimed to compare the effects of topical formulations containing essential oils (<strong>EOs</strong>) of chamomile, myrtle, , or a placebo on pain intensity and episiotomy wound healing in primiparous women.</p></div><div><h3>Methods</h3><p>This randomised and controlled clinical trial was conducted in the city of Hamadan in 2020. To this end, 120 parturient mothers were randomly assigned to one of three groups. They applied topical formulations containing 1% EO of chamomile, myrtle, or placebo twice daily for 10 days. Data collection was subsequently performed by assessing demographic, gynaecological, possible signs of side effects, pain intensity, and the wound-healing process 6 hours after surgery, days fifth and tenth after the intervention. In addition, the Visual Analogue Scale and Redness, Oedema, Ecchymosis, Discharge, and Approximation were utilised to examine pain intensity and healing processes. R software (v. 4.0.5) was employed for data analysis. The statistical significance level was <em>P</em> &lt; 0.05.</p></div><div><h3>Results</h3><p>There was no significant difference between groups at baseline. Furthermore, the mean pain intensity score in the three groups indicated no statistically significant differences. The group that received chamomile experienced the best wound-healing process (<em>P</em> &lt; 0.05) on the 10th day. Phytochemical investigations revealed the presence of α-bisabolol oxide A, α-bisabolol oxide B, β-farnesene, and chamazulene in chamomile EO, and α-pinene, 1,8-cineol, linalool, α-terpineol, linalool acetate, and limonene in myrtle EO.</p></div><div><h3>Conclusions</h3><p>A chamomile-containing formulation may promote episiotomy healing.</p></div>","PeriodicalId":56077,"journal":{"name":"Journal of Herbal Medicine","volume":"46 ","pages":"Article 100913"},"PeriodicalIF":2.2,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141636754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical analysis and in vitro anti-inflammatory, anticancer activities of Marrubium lutescens on melanoma cancer cell line and molecular docking studies","authors":"Cengiz Sarikurkcu,&nbsp;Sevim F. Erdoğmuş,&nbsp;Tuğba Yazar","doi":"10.1016/j.hermed.2024.100907","DOIUrl":"10.1016/j.hermed.2024.100907","url":null,"abstract":"<div><h3>Introduction</h3><p>The use of plants and their derivatives in the search for new therapeutic agents is on the increase every day due to their versatile applications.</p></div><div><h3>Methods</h3><p>Phytochemical analysis of <em>Marrubium lutescens</em> extract was carried out by liquid chromatography–electrospray tandem mass spectrometry (LC–ESI–MS/MS). Cytotoxicity of plant extract was determined by MTT assay on G361 cells. Moreover, the binding energetics of the two predominant compounds, rosmarinic acid and chlorogenic acid, against the target proteins microtubule affinity-regulating kinase 4 (MARK4) and human tyrosinase-related protein 1 (TYRP1) were also determined using molecular docking to gain molecular insights into the induced anti-cancer effect in G361. TAS, Total oxidant activity, and oxidative stress index of the cells lysates were determined. Also, TNF-α, TGF-β, DEF-β2, IL-1β cytokine levels were determined.</p></div><div><h3>Results</h3><p>Twenty distinct phytochemicals were determined and among the identified compounds, rosmarinic acid and chlorogenic acid as major components. Rosmarinic acid and chlorogenic acid have exhibited energetically highly favourable binding with intracellular target proteins MARK4 and TYRP1, as shown by their respective binding affinity values (Δ<em>G</em> <!-->=<!--> <!-->−7.97 kcal/mol; Δ<em>G</em> <!-->=<!--> <!-->−7.38 kcal/mol, respectively), suggesting further molecular evidence for the induced anti-cancer effect observed in the G361 cell line. Also, we have evaluated the anticancer prospective of <em>M. lutescens</em>. Anti-inflammatory mediators and oxidative stress parameters were increased to plant extract exposed cell line.</p></div><div><h3>Conclusions</h3><p>Reported results show that plant extract shown anticancer activity on G361 cells. According to the docking results, rosmarinic acid and chlorogenic acid, the two predominant compounds identified in the extract, may be responsible for the anti-cancer effect through synergistic action <em>via</em> prominent inhibition of MARK4 and TYRP1.</p></div>","PeriodicalId":56077,"journal":{"name":"Journal of Herbal Medicine","volume":"46 ","pages":"Article 100907"},"PeriodicalIF":2.2,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141574452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}