Determination of Antimicrobial, Antioxidant and Phenolic Properties of Onobrychis silvanensis sp. Nov. Endemic Species

Introduction

Onobrychis silvanensis sp. nov. (Fabaceae) is an endemic species and was collected for the first time from Diyarbakır (Silvan, Turkey). The study investigated the antimicrobial, antioxidant, and phenolic compounds of the species.

Methods

For this purpose, an O. silvanensis species extract was tested against two gram-positive (Staphylococcus aureus, Streptococcus pyogenes), two gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacteria, and Candida albicans fungus, for phenolic content, and antimicrobial and antioxidant capacity. Total phenolic content was measured spectrophotometrically, and the analytical validation parameter of the Liquid Chromatography with tandem mass spectrometry) method was used. DPPH method and copper (II) ion reduction method were used to measure total antioxidant capacity. Antimicrobial activity was determined using the minimum inhibition concentration microdilution method.

Results

Minimum inhibition concentration values obtained were 0.39 mg/ml for S. aureus, 0.39 mg/ml for S. pyogenes, 0.39 mg/ml for E. coli, 100 mg/ml for P. aeruginosa, and 6.25 mg/ml for C. albicans. DPPH activity was found to be 88.99% ± 0.373% at a concentration of 1 mg/ml. In the copper (II) ion reduction method, it was determined as 19.59 ± 0.167 mg TE/g at 1 mg/ml concentration. Total phenolic content values of ethanolic extracts of O. silvanensis species were found as 15 404 mg/g for Rutin, 11 694 mg/g for Quinic acid, and 10 385 mg/g for Miquelianin.

Conclusions

The results suggest an ethanolic extract of endemic species of O. silvanensis species showed high rate antimicrobial and antioxidant activity. It is thought that the study and results presented here will contribute significantly to the literature since these are the first records of this endemic species.