Austral Journal of Veterinary Sciences最新文献_第6页

Ultrasonographic study of the right coronary artery in performance horses 性能马右冠状动脉的超声研究

IF 0.9 4区农林科学

Austral Journal of Veterinary Sciences Pub Date : 2021-09-01 DOI: 10.4067/s0719-81322021000300139

Cristóbal A. Dörner, Constanza Chavarría

{"title":"Ultrasonographic study of the right coronary artery in performance horses","authors":"Cristóbal A. Dörner, Constanza Chavarría","doi":"10.4067/s0719-81322021000300139","DOIUrl":"https://doi.org/10.4067/s0719-81322021000300139","url":null,"abstract":"Historically, echocardiography has focused on the assessment of cardiac dimensions and indices of cardiac function. However, when referred to ultrasonographic coronary arteries characterisation in the horse, information is scarce. The purpose of this study was to describe the right coronary artery in both long and short axis and to analyse its variability between horses with different levels of performance. Sixty healthy horses were included in the study. The animals were eventing horses at different levels of performance and were allocated into two groups according to their athletic level. The internal lumen diameter and the area of the right coronary artery (RCA) were measured in the right parasternal long and short axis views in the 3rd and 4th intercostal space respectively, during systole and diastole. The results were compared between groups using the analysis of variance (ANOVA) and Student’s t-test. The correlation between the physiological parameters and the RCA was assessed using the Pearson correlation coefficient. Statistically significant differences were found when the RCA internal diameter was assessed between groups in both short and long axis. There were no statistically significant differences intra-group when short and long axis measurements were compared between each other. A positive correlation was observed between RCA and age, height, left atrium diameter, aortic valve diameter, sinus of Valsalva diameter, and aorta diameter. In conclusion, the level of training might affect the ultrasound measurement of the RCA in sport horses. Non-invasive echocardiography can be used to consistently assess the size of the right coronary artery in athletic horses in both short and long axis.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46341069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vaccination of nulliparous gilts against porcine epidemic diarrhoea can result in low neutralising antibody titres and high litter mortality 为未产仔接种猪流行性腹泻疫苗可导致低中和抗体滴度和高产仔死亡率

IF 0.9 4区农林科学

Austral Journal of Veterinary Sciences Pub Date : 2021-08-24 DOI: 10.4067/s0719-81322021000300147

Lourdes M. Galindo-Castañeda, R. Chacón-Salinas, V. Quintero, J. I. Sánchez-Betancourt, L. Cobos-Marín

{"title":"Vaccination of nulliparous gilts against porcine epidemic diarrhoea can result in low neutralising antibody titres and high litter mortality","authors":"Lourdes M. Galindo-Castañeda, R. Chacón-Salinas, V. Quintero, J. I. Sánchez-Betancourt, L. Cobos-Marín","doi":"10.4067/s0719-81322021000300147","DOIUrl":"https://doi.org/10.4067/s0719-81322021000300147","url":null,"abstract":"Porcine epidemic diarrhoea (PED) is a disease caused by an alphacoronavirus and the symptoms include watery diarrhoea and vomiting, with more than 80% mortality amongst newborn piglets. The placentation in sows hinders the transference of antibodies to the foetus, therefore, the vaccination of pregnant females and transference of antibodies to piglets through colostrum are essential to protect them against virus particles. The aim of the study was to determine whether vaccination of nulliparous gilts could induce a high colostrum antibody titre and lower litter mortality, in comparison with vaccinated multiparous sows previously exposed to the virus. Samples of colostrum were obtained from 11 nulliparous gilts with two previous vaccinations (inactivated vaccine) and from 9 multiparous sows with three or more vaccinations (inactivated vaccine) that had been exposed to the virus. The IgG antibody titre was determined through anti-PED enzyme-linked immunosorbent assays (ELISA) and the neutralisation of antibodies was evaluated through plaque reduction neutralisation tests (PRNT). The colostrum of nulliparous gilts, when compared to the multiparous sows, presented a lower anti-PED IgG antibody titre as well as fewer neutralising antibodies. Furthermore, the piglets of multiparous sows experienced higher survival in comparison with those of nulliparous gilts (P<0.01), and mortality was dependent on the ‘farrowing’ variable (P<0.01). In conclusion, these results show that vaccinating nulliparous gilts does not increase the survival of their piglets in comparison with multiparous sows and that the IgG titres and neutralising antibodies are significantly lower in the former. These results suggest that a modified vaccine strategy is needed for nulliparous gilts to increase piglet protection.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46101529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of antimicrobial resistant Salmonella enterica strains in samples of ground hedgehogs (Atelerix albiventris) reared as pets in the urban area of Santiago, Chile 智利圣地亚哥市区饲养的地面刺猬（Atelerix albiventris）样本中抗微生物肠炎沙门氏菌菌株的检测

IF 0.9 4区农林科学

Austral Journal of Veterinary Sciences Pub Date : 2021-05-26 DOI: 10.4067/S0719-81322021000200133

S. Pérez, Marlen Barreto, P. Retamal

{"title":"Detection of antimicrobial resistant Salmonella enterica strains in samples of ground hedgehogs (Atelerix albiventris) reared as pets in the urban area of Santiago, Chile","authors":"S. Pérez, Marlen Barreto, P. Retamal","doi":"10.4067/S0719-81322021000200133","DOIUrl":"https://doi.org/10.4067/S0719-81322021000200133","url":null,"abstract":"The breeding of exotic pets has become a popular practice in Chile and, within this group of animals, small mammals such as guinea pigs and hedgehogs have gained importance due to their docile behaviour. The most common exotic hedgehog species in Chile is the African pygmy hedgehog (Atelerix albiventris). It has been reported that these pets are reservoirs of some zoonotic pathogens, among which Salmonella enterica constitutes an important threat for the owners. This study aimed to detect the presence of Salmonella strains in faeces from hedgehogs (Atelerix albiventris) admitted to a veterinary clinic in Santiago, Chile, and to characterise the antimicrobial susceptibility of the isolated strains. From 200 animals sampled, S. enterica was detected in 5 hedgehogs, corresponding to serotypes Muenchen (2), Infantis (2) and IV43:z4,z23:- (1). Furthermore, phenotypic antimicrobial resistance was determined in all subsp. enterica isolates. These results suggest that in Chile these exotic pets constitute a potential hazard for public health, therefore, supporting educational campaigns about basic biosecurity measures is necessary, mostly aimed at pet owners and risk groups.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43907057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic response to water shortage in an isolated feral sheep population 孤立野生绵羊群体对缺水的代谢反应

IF 0.9 4区农林科学

Austral Journal of Veterinary Sciences Pub Date : 2021-05-26 DOI: 10.4067/S0719-81322021000200091

Ornar R. Prado, Erika Arias, M. Carrillo, Juan R. Hernández, Arturo C. García

{"title":"Metabolic response to water shortage in an isolated feral sheep population","authors":"Ornar R. Prado, Erika Arias, M. Carrillo, Juan R. Hernández, Arturo C. García","doi":"10.4067/S0719-81322021000200091","DOIUrl":"https://doi.org/10.4067/S0719-81322021000200091","url":null,"abstract":"To establish metabolic responses for biochemical analytes related to freshwater shortage adaptation, a total of 376 blood samples were collected from feral sheep at the Socorro Island, Revillagigedo Archipelago. Comparisons were made between four sampling periods with repeated measurements at 0, 7, 14, and 21 d (94 blood serums: 84 females and 10 males). During the first week, the sheep received daily water ad libitum. During the second and third week, the sheep received 60% daily water in relation to the first 7 days intake. Analysis of variance was used to compare the mean values between sampling days. Stepwise regression analysis was used to evaluate the relationships between the biochemical analytes. The glucose (GLU), total cholesterol (COL-T), triglycerides (TAG), urea, albumin (ALB), total protein (PROT-T), sodium ion (Na+), creatine kinase (CK), arginine vasopressin (AVP), and aldosterone (Aldo), were determined. With the exception of GLU, the COL-T, TAG, urea, ALB, PROT-T, Na+, CK, AVP, and Aldo showed differences between sampling days with the higher values corresponding to 14 d with limited water intake. Negative correlations (P<0.05) between ALB with COL-T and TAG, were quantified. Positive correlations (P<0.05) between COL-T with TAG, and Aldo; between urea with PROT-T, between CK with ALB and PROT-T, between AVP with COL-T, TAG, urea, PROT-T and Aldo, and between Aldo with Na+, were quantified. Results could help improve the accuracy of metabolic profiles used as a tool for evaluating dehydration indicators and to describe the physiological mechanisms employed by feral sheep to cope with limited availability of freshwater.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47844635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of Mycobacterium avium subsp. paratuberculosis infection in sheep flocks from three regions of Antioquia, Colombia 鸟分枝杆菌亚型的患病率。哥伦比亚安蒂奥基亚三个地区绵羊群的副结核感染

IF 0.9 4区农林科学

Austral Journal of Veterinary Sciences Pub Date : 2021-05-06 DOI: 10.4067/S0719-81322021000200083

Miguel Hernández-Agudelo, Bernardita Collado, C. Tejeda, Nicolás F. Ramírez-Vásquez, J. Fernández-Silva, M. Salgado

{"title":"Prevalence of Mycobacterium avium subsp. paratuberculosis infection in sheep flocks from three regions of Antioquia, Colombia","authors":"Miguel Hernández-Agudelo, Bernardita Collado, C. Tejeda, Nicolás F. Ramírez-Vásquez, J. Fernández-Silva, M. Salgado","doi":"10.4067/S0719-81322021000200083","DOIUrl":"https://doi.org/10.4067/S0719-81322021000200083","url":null,"abstract":"Paratuberculosis or Johne’s disease is a slow-developing infectious disease caused by Mycobacterium avium subsp. paratuberculosis (MAP) affecting mainly domestic ruminants and producing a significant economic threat to livestock production systems. Although reports on paratuberculosis in small ruminants in Colombia are very scarce, the Colombian sheep industry has identified paratuberculosis as one of the causes of its low development. There have been reports of MAP infection in sheep flocks, mainly in the Cundiboyacense Plateau and the Bogota savannah, but the prevalence of MAP infection in sheep and goat populations in Colombia is yet unknown. Therefore, the present study aimed to accurately estimate the prevalence of MAP infection at flock level in a sheep population of 24 flocks located in three regions of the province of Antioquia, Colombia. ELISA test as well as culture and direct qPCR were used as diagnostic tools. Overall, 456 blood serum samples were analysed and at least one seropositive animal was found in 17 (70% IC: 51.2-0.90) out of the 24 study flocks and, in total, 37 animals showed positive ELISA results (8% IC: 5.5-10.5). Regarding MAP direct detection, 90 faecal pools from the 24 flocks were cultured and subjected to qPCR diagnosis. Both direct qPCR and culture detected 25 (27.7%) and 64 (71.1%) faecal pools as MAP positive, respectively. More specifically, MAP positive pools were detected in 45.8% (IC: 24.3-67.3) and 83.3% (IC: 67.3-99.3) of the flocks by direct qPCR and culture, respectively. MAP infection is widespread in sheep flocks in the study regions and the combination of several diagnostic tests was necessary to achieve a more accurate and precise infection detection of this important pathogen.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49522878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterisation and antibiotic sensitivity profile of Pasteurella multocida isolated from poultry farms in Malaysia 从马来西亚家禽养殖场分离的多杀性巴氏杆菌的分子特征和抗生素敏感性

IF 0.9 4区农林科学

Austral Journal of Veterinary Sciences Pub Date : 2021-05-06 DOI: 10.4067/S0719-81322021000200121

Mohammad A. Sabsabi, Z. Zakaria, J. Abu, N. Faiz

{"title":"Molecular characterisation and antibiotic sensitivity profile of Pasteurella multocida isolated from poultry farms in Malaysia","authors":"Mohammad A. Sabsabi, Z. Zakaria, J. Abu, N. Faiz","doi":"10.4067/S0719-81322021000200121","DOIUrl":"https://doi.org/10.4067/S0719-81322021000200121","url":null,"abstract":"Fowl cholera has caused significant economic losses in many poultry producing countries worldwide. In Malaysia, outbreaks of fowl cholera are frequently reported and encountered in different types of poultry productions. The objective of this study was to characterise 13 avian Pasteurella multocida, isolated from fowl cholera outbreaks in Central Peninsular Malaysia in the period between 2000 and 2018. The isolates were subjected to multiplex polymerase chain reaction (PCR) for capsular serotyping, disc diffusion method for antimicrobial susceptibility profiles, and molecular genotyping using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The capsular serotyping showed all 13 Pasteurella multocida isolates belonging to capsular serotype A. The antimicrobial susceptibility showed several multidrug resistance strains among the P. multocida isolates. All the isolates were resistant to erythromycin (100%), streptomycin (68%), tetracycline (37%), enrofloxacin (37%), florfenicol (23%), penicillin G (14%), gentamicin (14%), and amoxicillin (14%). The PFGE analysis clustered the isolates into three clones. Group A included isolates with a similarity of 87% from the year 2000, 2013, and 2018. Three sequence types were identified using MLST typing namely, ST129, ST231, and ST355. The ST355 was assigned for the first time in the Rural Industries Research and Development Corporation (RIRDC) database. Besides, ST129 has been reported in India, China, and Sri Lanka, which highlights the possibility of transmission between Asian countries. This study provides an insight into epidemiological information of Pasteurella multocida that causes fowl cholera outbreaks in the central region of Peninsular Malaysia.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45531486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Is the effectivity of copper ions treatment of milk enough to block Mycobacterium avium subsp. paratuberculosis infection in calves? 铜离子处理牛奶的效果是否足以阻断鸟分枝杆菌亚种?小牛的副结核感染?

IF 0.9 4区农林科学

Austral Journal of Veterinary Sciences Pub Date : 2021-05-06 DOI: 10.4067/S0719-81322021000200115

P. Steuer, C. Tejeda, M. Moroni, J. Soto, M. Salgado

{"title":"Is the effectivity of copper ions treatment of milk enough to block Mycobacterium avium subsp. paratuberculosis infection in calves?","authors":"P. Steuer, C. Tejeda, M. Moroni, J. Soto, M. Salgado","doi":"10.4067/S0719-81322021000200115","DOIUrl":"https://doi.org/10.4067/S0719-81322021000200115","url":null,"abstract":"Milk is an important transmission route of Mycobacterium avium subsp. paratuberculosis (MAP) for dairy calves. Given its resistance to pasteurization, alternative milk treatments are needed to control MAP transmission via milk. The present study reports the evaluation of a novel milk decontamination treatment based on copper ions as a means of preventing infection in dairy calves. Ten newborn calves were assigned to one of two experimental groups (n=5) which were studied for 1 year. The first group was fed milk naturally contaminated with MAP and the second one received the same milk but after being treated with copper ions. In both groups, milk MAP load was estimated. The progression of the infection was monitored monthly and at the end of the study, calves were euthanised, and tissue samples were examined both grossly and by histopathology. The treatment of milk with copper ions significantly reduced the number of viable MAP. Faecal shedding of MAP was observed in both study groups, but the calves fed naturally contaminated milk began to shed MAP earlier. Only calves fed copper-treated milk showed histopathological evidence consistent with MAP infection. The latter offers more questions than answers, and maybe the presence of a more tolerant and virulent MAP strain could be the final answer to this situation.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45637885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Aquatic pollution from anthropogenic discharges modulates gene expression in liver of rainbow trout (Oncorhynchus mykiss) 人为排放的水生污染调节虹鳟肝脏中的基因表达

IF 0.9 4区农林科学

Austral Journal of Veterinary Sciences Pub Date : 2021-05-06 DOI: 10.4067/S0719-81322021000200099

Guillermo E. Valenzuela-Nieto, C. Leal, J. Schwaiger, H. Ferling, L. Vargas-Chacoff, G. Kausel

{"title":"Aquatic pollution from anthropogenic discharges modulates gene expression in liver of rainbow trout (Oncorhynchus mykiss)","authors":"Guillermo E. Valenzuela-Nieto, C. Leal, J. Schwaiger, H. Ferling, L. Vargas-Chacoff, G. Kausel","doi":"10.4067/S0719-81322021000200099","DOIUrl":"https://doi.org/10.4067/S0719-81322021000200099","url":null,"abstract":"With the aim to characterise the biological impact of anthropogenic discharge in a river, gene expression in fish was evaluated as a biomarker for mixture effects of potential toxic compounds in the aquatic environment. Adult male rainbow trout (Oncorhynchus mykiss) were used as monitoring organisms. Within the German experimental set up, trout were exposed actively for 28 days in biologically purified sewage as well as in river water up- and downstream of the sewage treatment plant (STP) effluent. In a different approach, wild trout were captured in southern Chile near anthropogenic discharges. Fish were taken from river Maullin receiving sewage from a settlement and from river Pescado influenced by effluents from a fish farm. In vivo effects were assessed by RT-qPCR analyses of biomarker gene expression, vitellogenin (vg), metallothionein (mt) and cytochrome 1A (cyp1A) in liver samples with primers amplifying specific sequences previously confirmed by cloning and sequencing. The modulation of expression of marker genes involved in metal stress, reproduction and detoxifying systems in the liver of male rainbow trout revealed organismal response to anthropogenic contamination in two different study areas, such as Chile and Germany, thereby indicating a potential risk on the aquatic ecosystems.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49606286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dairy cow behaviour around calving: Its relationship with management practices and environmental conditions 奶牛产仔行为与管理实践和环境条件的关系

IF 0.9 4区农林科学

Austral Journal of Veterinary Sciences Pub Date : 2021-01-01 DOI: 10.4067/S0719-81322021000100009

Fabiola Matamala, A. Strappini, P. Sepúlveda-Varas

{"title":"Dairy cow behaviour around calving: Its relationship with management practices and environmental conditions","authors":"Fabiola Matamala, A. Strappini, P. Sepúlveda-Varas","doi":"10.4067/S0719-81322021000100009","DOIUrl":"https://doi.org/10.4067/S0719-81322021000100009","url":null,"abstract":"Calving is one of the most challenging and painful experiences for dairy cattle and a process that involves coping with physical and physiological changes, as well as environmental and management-related stressors. In recent years, it has been argued that the application of cow behaviour knowledge might facilitate their efficacious management during calving. This review aims to summarise and discuss current knowledge regarding the behavioural changes that occur around calving time. The relationship between calving behaviour, management practices, and environmental conditions in dairy cattle raised in intensive indoor production systems, as well as pasture-based systems, is also discussed. First, we briefly outline the process of parturition and the concept of maternal behaviour. We then describe behavioural changes that occur around parturition in normal and dystocic births and how variations in these behaviours can be used to predict normal or assisted calving in dairy cattle; particular emphasis is placed on the role of feeding, rumination, and lying behaviour. Finally, we review how management practices and environmental conditions can influence cow’s behaviour at calving and discuss the importance of providing an environment that accommodates the behaviour they are motivated to perform. This review presents evidence that the time a cow is moved to the calving area, the type of group housing and the provision of a secluded area to calve, can impact the behavioral responses of dairy cows at calving. Evidence regarding the effects of exposure to environmental conditions such as heat during summer, and/or cold, wet and mud during winter can also have a negative impact on behaviour, suggesting potential benefits of providing cows with a protected area to calve. We conclude that a better understanding of the behaviour of parturient cows may help producers improve the care and management around calving time.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42606454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Mycoplasma isolation in milk samples from dairy herds in Chile 智利奶牛群牛奶样本中支原体的分离

IF 0.9 4区农林科学

Austral Journal of Veterinary Sciences Pub Date : 2021-01-01 DOI: 10.4067/S0719-81322021000200109

F. Ulloa, J. Soto, J. Kruze, A. Mella

{"title":"Mycoplasma isolation in milk samples from dairy herds in Chile","authors":"F. Ulloa, J. Soto, J. Kruze, A. Mella","doi":"10.4067/S0719-81322021000200109","DOIUrl":"https://doi.org/10.4067/S0719-81322021000200109","url":null,"abstract":". Mycoplasma bovine mastitis is a highly contagious disease, usually associated with clinical cases refractory to antibiotic treatment. The aim of this study was the isolation of Mycoplasma species in cattle milk samples from dairy herds in Chile. Bulk tank milk samples selected by convenience from 91 Holstein Friesian dairy herds located in Los Rios (66) and Los Lagos (25), the two most important dairy Regions in the country, were collected. Additionally, 100 individual milk samples from cows with a high incidence of clinical mastitis, refractory to antibiotic therapy, and negative bacteriological results for traditional mastitis pathogens, all from the Biobío Region and received in our diagnostic laboratory, were included. All samples were cultured for 10 days on PPLO medium. The differentiation of suspect colonies between genus Mycoplasma and Acholeplasma was performed by the digitonin test and a specific PCR. The species identification was performed by a M. bovis specific PCR and 16S rRNA sequencing. Mycoplasma was isolated from 3 (3.3%) bulk tank milk samples and 2 (2%) individual cow milk samples. All colonies were identified as Mycoplasma by the digitonin test and by a specific PCR. At species level, one strain isolated from a bulk tank milk sample was identified as M. bovis . The remaining two strains isolated from bulk tank milk samples were identified as M. bovigenitalium , while the two strains isolated from milk of individual cows were identified as M. alkalescens . These results show that not only M. bovis is present in Chilean dairy herds, but also other pathogenic species not previously described in Chile such as M. bovigenitalium and M. alkalescens , which pose a potential risk for dairy herds in southern Chile.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70331420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0