Indonesia Journal of Biomedical Science最新文献

{"title":"Potential diagnostic biomarkers for early detection of idiopathic sensorineural hearing loss in type 2 diabetes mellitus patients","authors":"A. Santosa, K. Suastika, Made Ratna Saraswasti, Wayan Suardana, I. Sujaya","doi":"10.15562/ijbs.v16i1.371","DOIUrl":"https://doi.org/10.15562/ijbs.v16i1.371","url":null,"abstract":"Background: Sensorineural hearing loss (SNHL) is a hearing loss that accounts for almost 90% of all hearing loss. Various factors can cause this disorder, one of which is diabetes mellitus (DM). However, few studies still discuss biomarkers to detect the incidence of SNHL in patients with DM as early as possible. The purpose of this paper is to determine the potential of biomarkers to detect SNHL in type 2 diabetes mellitus (T2DM) patients.\u0000Methods: The author uses a database in the form of PubMed, Cochrane, and ScienceDirect used to search for articles using the Boolean operator \"AND\" or \"OR\" using the keywords \"hearing loss,\" \"diabetes,\" \"biomarkers,\" \"Prestin,\" \"lipid profile,\" and \"Nox2\". A total of 276 studies were obtained, followed by the title and abstract screening and feasibility assessment (eligibility) to get inclusion studies included in the qualitative analysis (systematic study).\u0000Result: A total of 10 inclusion studies consisted of 2 cross-sectional studies, 2 case controls, and six preclinical studies with 105 and 101 patients in the cross-sectional and case-control studies, respectively. A total of 5, 3, and 2 studies reported associations between hearing loss and Prestin, Nox2, and lipid profile values, respectively.\u0000Conclusion: The biomarkers such as Prestin, Nox2 and lipid profile have a role in the early diagnosis of SNHL, but no related studies discuss T2DM. Therefore, basic and clinical studies are still needed to determine its effectiveness.","PeriodicalId":55769,"journal":{"name":"Indonesia Journal of Biomedical Science","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87108493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality of defecation in patients with anorectal malformation following posterior sagittal anorectoplasty (PSARP) in Arifin Achmad General Hospital of Riau Province, Indonesia","authors":"Tubagus Odih Rhomdani Wahid, Ratu Astuti Dwi Putri","doi":"10.15562/ijbs.v16i1.385","DOIUrl":"https://doi.org/10.15562/ijbs.v16i1.385","url":null,"abstract":"Background: Anorectal malformation is a congenital abnormality that shows imperfect anus or absence of anus. The most common complication in patients with anorectal malformations after posterior sagittal anorectoplasty (PSARP) are disorders in defecation, especially constipation.\u0000Method: The purpose of this study was to determine the correlation of quality of defecation measured with the presence of constipation with the type of PSARP surgery in Arifin Achmad Hospital of Riau Province. This study used an analytical retrospective method from November 2018 to March 2019. Total sampling was the sampling method used in this study, which accounted for a total of 28 subjects. The population of this study was all patients with anorectal malformation and fistula in the Pediatric Surgery Division of Arifin Achmad General Hospital.\u0000Result: This study showed that among 28 cases found, there were 19 female patients (68%) and 9 male patients (32%). Based on the lesion level, there were 17 cases (60,7%) of intermediate lesions and 11 cases (39,3%) of high lesions. In this study, rectovestibular fistula had the highest incidence among other types of fistula (53,6%). Constipation were found in 53.6% patients with p-value of 0.025 (OR 6.40, 95% CI 1.183-34.613).\u0000Conclusion: There was a significant correlation between the PSARP method and constipation in patients who underwent anorectal malformation surgery, which constipation was a sign of the quality of defecation.","PeriodicalId":55769,"journal":{"name":"Indonesia Journal of Biomedical Science","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82554479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Body mass index inversely associated with bone microarchitecture quality: a systematic review and meta-analysis","authors":"K. Kusuman, Kadek Adit Wiryadana, I. Setiawan, Su Djie To Rante","doi":"10.15562/ijbs.v16i1.383","DOIUrl":"https://doi.org/10.15562/ijbs.v16i1.383","url":null,"abstract":"Introduction: The rising prevalence of obesity forces the orthopedist to consider it in fracture risk assessment. Multiple studies have consistently demonstrated that people with obesity have increased bone mineral density (BMD). Although it appears other factors in bone strength may influence the fracture risk, including bone microarchitecture, which recently can be measured by trabecular bone score (TBS). The complex associations between TBS and BMI remain unclear, and some studies show inconsistent findings. This systematic review and meta-analysis aimed to understand whether increased BMI is associated with lower TBS by indirectly pooling all the available evidence from the published literature.\u0000Methods: A literature search was carried out using PubMed, Cochrane Library, Google Scholar and other popular journal databases using the terms \"trabecular bone score\", \"body mass index\" and the possible synonyms. We extracted the total sample, mean and standard deviation of TBS for patients within each BMI category from the selected literature. A meta-analysis was conducted using a random-effects model and inverse variant methods to synthesize the pooled effect size (mean difference) for each gender subgroup.\u0000Results: After an initial search and screening of 2399 studies, seven reports published between 2016-2019 were included (five cross-sectional, one cohort, and one randomized clinical trial). These include 2872 samples which were mostly women (2286). One thousand thirty-one samples were with normal BMI, 1124 samples were with overweight BMI, and 717 samples were with obesity. The included studies varied by age group and gender. The between-study heterogeneity with I2 index ranging 0%-76% studies in man showed higher heterogeneity. Compared with normal individuals, those with overweight and obesity had lower TBS with a mean difference of -0.02 (95% CI -0.03 to -0.01) and -0.07 (95% CI, -0.09 to -0.05), respectively. The differences were consistent across gender, although larger differences were found in men.\u0000Conclusion: Individuals with higher BMI have a lower TBS than individuals with normal BMI in a stepwise manner. It suggests that the inclusion of TBS can improve the assessment of fracture risk in obese individuals.","PeriodicalId":55769,"journal":{"name":"Indonesia Journal of Biomedical Science","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77586755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple Antimicrobial-Resistant Bacteremia and Fungal Infection Pneumonia in Elderly Patient: A Case Study","authors":"I. G. A. A. Indrayuni, I. G. A. A. I. Anteja, I. Aryana","doi":"10.15562/ijbs.v16i1.388","DOIUrl":"https://doi.org/10.15562/ijbs.v16i1.388","url":null,"abstract":"Introduction: The spread of multidrug-resistant bacterial pathogens has become alarming globally. Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (E. coli) have been recognized as a major multidrug-resistant bacteria implicated in hospital and community-acquired infections worldwide.Case: We present the case of an elderly male, 74-year-old, with multiple comorbidities, who came to the hospital with main symptoms of cough. Three months previously, he was diagnosed with pneumonia. Nevertheless, he was treated as an outpatient, with Cefixime prescribed as an oral antibiotic. His non-contrast chest CT Scan showed opacities at the upper zone, middle zone and lower zone of the left lung and the lower zone of the right lung. The patient’s symptoms and imaging expertise strongly suggest a pulmonary infection. Sputum culture and sensitivity test result eventually confirmed positive E. coli isolates demonstrated resistance to Augmentin, Ceftazidime, Gentamicin, Ampicillin sulbactam, Aztreonam, Cefotaxime, Cefepime. Fungal culture by sputum specimen revealed growth of Candida, non-Candida albicans. Concurrently, history of hospitalization and antibiotic treatment exposure are considered risk factors for the acquisition of multidrug resistance in this patient. Furthermore, corticosteroids in elderly patients are alleged as a risk factor of fungal infection.Conclusion: The increasing case of antimicrobial resistance in elderly patients is challenging for the clinician. Culture and sensitivity tests performed before starting antimicrobial treatment in hospitalized geriatric patients can reduce unnecessary and inappropriate antimicrobial use. Fungal test is considered to be done in a susceptible case of the elderly patients. The rapid diagnosis followed with prompt initiation of appropriate antibiotic and antifungal therapy is urgently required. ","PeriodicalId":55769,"journal":{"name":"Indonesia Journal of Biomedical Science","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78820897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High Bioscore predicts metastasis in breast cancer patients in Sanglah General Hospital, Denpasar, Bali, Indonesia","authors":"Ika Ariandana, I. B. Suryawisesa, I. K. Widiana","doi":"10.15562/ijbs.v16i1.327","DOIUrl":"https://doi.org/10.15562/ijbs.v16i1.327","url":null,"abstract":"Background: Risk determination of breast cancer metastases is important for clinicians to be able to estimate the adequate therapy for the patients provide the basis for clinical reasoning and explanation of the possibility of metastasis to breast cancer patients who are in treatment. However, current prediction models have low accuracy and molecular determinants tend to have a high evaluation cost. Bioscore had emerged as a new reliable option in determining the risk of metastases in breast cancer, but it is not widely applied yet. Therefore, it is necessary to study its capability in determining risk of metastasis in the clinical setting in order to validate its future application.Objective: To determine the bioscore scoring system as a risk determination for metastases in breast cancer at Sanglah Hospital, Denpasar.Methods: This research was a nested case control study involving 32 subjects in two groups, namely metastatic and non-metastatic breast cancer groups. All patients are registered in the Cancer Registry Data at Sanglah Central General Hospital. The parameters in this study were age, stage, grading, hormonal status, HER2 status, menopausal status, metastatic status and bioscore values. Data were tabulated and statistically analyzed using SPSS 25.Results: From all subjects, the average patient age of 51.50 (±8.491) years, ranging from 31 - 72 years old. The bioscore was significantly associated with all research variables. Risk analysis showed that breast cancer patients with a high bioscore (5-7) has a significantly higher risk of metastasis (OR: 21.00; 95% CI: 5.620 – 78.475) compared to those with low bioscore (1-4).Conclusion: The bioscore scoring system may have a significant value in predicting metastasis in breast cancer patients. However, its application still needs further and more comprehensive studies to validate its application in breast cancer management.","PeriodicalId":55769,"journal":{"name":"Indonesia Journal of Biomedical Science","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79727122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of follicular adenoma patients at H. Adam Malik General Hospital, Medan, during the 2017-2020 period","authors":"L. I. Laksmi, Betty Betty, S. Hilda","doi":"10.15562/ijbs.v16i1.375","DOIUrl":"https://doi.org/10.15562/ijbs.v16i1.375","url":null,"abstract":"Background: Follicular adenoma is a benign tumor enclosed by a capsule with follicular differentiation, more common in women and usually occurs in adulthood, and is more common in iodine deficiency. The average tumor size is 1-3 cm. Follicular neoplasms do not have features of papillary thyroid carcinoma and no capsule and/or vascular invasion were found. This study aims to evaluate the characteristics of follicular adenoma patients at H. Adam Malik General Hospital, Medan, during the 2017-2020 periodMethods: This study is a descriptive study with a cross-sectional approach on 31 samples based on medical record data diagnosed as follicular adenoma. After reviewing the slides, 23 samples were obtained that met the inclusion and exclusion criteria. Samples were assessed according to age, sex, tumor size, number of nodules, growth patterns, and histopathological variants. Data were analyzed using SPSS version 26 for Windows.Results:  Cases of follicular adenoma at H. Adam Malik Hospital Medan during 2017-2020 with an average age of 46.7 years. The youngest age was 20 years and the oldest age was 71 years, with the highest age group being 50-59 years (26.1%). Most gender was female (87.0%) than male (13.0%). The most common tumor size was above 4 cm (69.6%). The highest number of nodules was found in 1 nodule (solitary or unilateral) (52.2%). The most common growth pattern in microscopic preparations was macrofollicular adenoma (65.2%), with the most histopathological variant being conventional follicular (91.3%).Conclusion: Benign lesions such as adenomas are often misdiagnosed due to the lack of further studies on the histopathological criteria. Follicular adenomas can be treated with lobectomy and have a good prognosis if diagnosed early and with proper treatment.","PeriodicalId":55769,"journal":{"name":"Indonesia Journal of Biomedical Science","volume":"295 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79576476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of Stromal Cell-Derived Factor 1 (SDF-1) and Transforming Growth-Factor-Beta 1 (TGF-?1) levels in Packed Red Cells (PRC) as the indicator of storage lesion at Sanglah Hospital-Blood Bank, Bali, Indonesia","authors":"A. A. Lestari, N. Mulyantari, I. Prabawa","doi":"10.15562/ijbs.v16i1.374","DOIUrl":"https://doi.org/10.15562/ijbs.v16i1.374","url":null,"abstract":"Background: Packed Red Cells (PRC) is one of the blood component obtained from the whole blood through the removal of blood plasma. The storage of PRC aims to maintain the viability and function of erythrocytes by reducing cell metabolic activity. However, PRC's biochemistry changes could occur through storage lesion by releasing Stromal Cell-Derived Factor 1 (SDF-1) and Transforming Growth Factor-Beta 1 (TGF-?1) that affect the erythrocytes metabolism and viability prior to transfusion. This study aims to evaluate the increase of SDF-1 and TGF-?1 levels in PRC as the indicator of storage lesion at Sanglah Hospital-Blood Bank, Bali, Indonesia.Methods: An observational cross-sectional study using randomized post-test only without control group design was conducted at Sanglah Hospital-Blood Bank, Bali. The consecutive random sampling technique was used in this study to the PRC, who met the inclusion criteria. Samples of the SDF-1 group (N=84) were divided into 3 such as Group 1 (1-7 days), Group 2 (8-14 days), and Group 3 (15-21 days). However, 53 PRC was divided into 2 groups as Group 1 (1-7 days) and Group 2 (8-14 days).  The SDF-1 and TGF-?1 levels were evaluated for each group using Human SDF-1/CXCL12 and TGF-?1 Kit from Elabcience® by ELISA method.  Data were analyzed using SPSS version 25 for Windows software.Results: The median levels of SDF-1 in Group 1 group was 0.385 (0.050-1.020) ng/mL, followed by 0.455 (0.330-0.750) ng/ml in Group 2, and 0.490 (0.380-0.920) ng/ml in Group 3 (p<0.05). The mean of TGF-?1 levels was slightly higher in Group 1 (2,365.21±715.96 pg/ml) compared with Group 2 (2,210.39±994.68 pg/ml) (p=0.523). There was a significant difference in SDF-1 levels in Group 1-Group 2 (P=0.041) and Group 1-Group 3 (P=0.000) groups; however, no significant difference was found in Group 2-Group 3 (P=0.133) using the Mann-Whitney test. The Spearman's rho test found a moderate positive correlation between SDF-1 levels and storage time by day (r=0.546; P=0.000). In addition, based on the Pearson correlation test, there was a weak positive correlation between TGF-?1 levels and storage time by day (r=0.461; p=0.001).Conclusion: The increase of SDF-1 and TGF-?1 levels in PRC by days of storage time could be used as the indicator for storage lesion at Sanglah General Hospital-Blood Bank, Bali, Indonesia.","PeriodicalId":55769,"journal":{"name":"Indonesia Journal of Biomedical Science","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84866512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maintaining the quality of problem-based learning in developing countries during the pandemic era","authors":"Ida Bagus Kerthyayana Manuaba, G. P. Supadmanaba, I. S. Darmayani, M. Yani, N. Remitha, I. G. A. P. Sasmana, I. G. A. H. Sundariyati, D. A. Sindhughosa","doi":"10.15562/ijbs.v16i1.378","DOIUrl":"https://doi.org/10.15562/ijbs.v16i1.378","url":null,"abstract":"The COVID-19 pandemic is referred to as an unpredictable phenomenon and a challenge that stimulates change, one of which is in the field of medical education. Online learning as the main solution in the field of education certainly offers positive and negative impacts for medical students. Therefore, learning design is a strategy that continues to be developed in improving the quality of teaching that is more effective and innovative. The purpose of writing this review is to find out more about the quality of problem-based learning in developing countries during the pandemic era. This type of study is a literature review that uses the literature study method. The sources used in this review consist of relevant journals from search engines such as Pubmed, Google Scholar, ScienceDirect, and Cochrane Library. The results of the literature search show that pandemic conditions encourage learning to be carried out using virtual methods. To implement online learning during this pandemic, various technologies have been developed, ranging from digital problem-based learning (PBL) which has been implemented at the National Taiwan University College of Medicine, web video conferencing learning (WVC) at King Abdulaziz University in Saudi Arabia which is effectively used in the case-based discussion (CBD) process, as well as various platforms that support online learning, such as Tencent Meeting, DingTalk, and Edmodo. EdTech Start-ups and SWAYAM portal in India also aims to maintain equitable distribution, access, and quality in times of pandemic. Some applications that can support live streaming based on video conferencing are Microsoft Teams, Google Meet, Lark, Zoom. Seeing the various advantages and problems faced in implementing online lectures during the pandemic, various improvement strategies need to be carried out in maintaining the quality and learning outcomes, starting from infrastructure, personnel, methods in delivering lectures, learning outcomes, institutional responses, and support from the government.","PeriodicalId":55769,"journal":{"name":"Indonesia Journal of Biomedical Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88584672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Relationship Between FokI Vitamin D Receptor Polymorphisms on The Incidence Of Diabetic Retinopathy In Type 2 Diabetes Mellitus Patients At Sanglah Hospital Denpasar Bali","authors":"Theressia Handayani, A. E. Darwinata, D. Wihandani, I Gede Putu Supadmanaba","doi":"10.15562/ijbs.v15i2.333","DOIUrl":"https://doi.org/10.15562/ijbs.v15i2.333","url":null,"abstract":"Introduction: Complications of T2DM result in many organs of the body including visual disturbances (diabetic retinopathy). Polymorphisms in vitamin D receptor gene FokI, increase the risk of complications of T2DM including DR. This study aims to determine the FokI polymorphism in vitamin D receptor gene in DMT2 patients at Sanglah Hospital Denpasar Bali and to determine whether there is a relationship between vitamin D receptor polymorphisms FokI on the incidence of DR in DMT2 patients at Sanglah Hospital Denpasar Bali.\u0000Methods: Design of the research was a cross-sectional analytical study. To detect the status of the FokI polymorphism, DNA segment amplification was performed using the PCR method using a primer specific for the FokI polymorphism. Furthermore, sequencing was performed to detect the base pattern at the location of the FokI SNP. Statistical analysis was performed by using SPSS 25.sav. P-value <0.05 was considered statistically significant.\u0000Results: There were 38 samples that were eligible to be analysed and successfully sequenced. The mean age of the sample was 50.68 years with the proportion of DR and non-DR of 73.7% and 26.3%, with the majority of DR patients being male (65.8%). In our multivariate analysis, we found a significant relationship between VDR FokI polymorphism with hypertension among diabetic retinopathy patient (p=0.036; adjusted OR=18; 95% CI=1.694-191.233).\u0000Conclusion: Heterozygous (C/T) VDR Fok I polymorphism was the most frequent in this finding. There is no significant relationship between FokI polymorphisms in vitamin D receptor gene and the incidence of RD in T2DM patients at Sanglah Hospital Denpasar.","PeriodicalId":55769,"journal":{"name":"Indonesia Journal of Biomedical Science","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88473614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship Between Vitamin D Receptor TaqI Polymorphism and the Clinicopathological Features of Breast Cancer Patients at Sanglah Hospital, Denpasar, Bali","authors":"Cynthia Lawrence, A. E. Darwinata, D. Wihandani, I Gede Putu Supadmanaba","doi":"10.15562/ijbs.v15i2.349","DOIUrl":"https://doi.org/10.15562/ijbs.v15i2.349","url":null,"abstract":"Introduction: Breast cancer is a malignant tumor of the mammary gland. Breast cancer is associated with vitamin D receptor (VDR) TaqI polymorphism. This study aims to prove the relationship between TaqI polymorphism and the clinicopathology of breast cancer in Bali.\u0000Methods: This study is an observational analytic study with a cross-sectional design conducted from February to August 2021. A total of 39 sample DNA isolates from breast cancer patients at Sanglah Hospital were enrolled in the study. The independent variable was the VDR TaqI gene polymorphism, while the dependent variable was the clinicopathological breast cancer (clinical stage and histological grading). The examination was carried out by PCR and sequencing. Clinical staging and histological grading of breast cancer were taken from the patient's medical record. The results were analyzed using descriptive and bivariate analysis.\u0000Results: TaqI polymorphism of breast cancer at Sanglah Hospital Denpasar was dominated by the wild type TT genotype as much as 59%. This study did not find the CC genotype. The results of the statistical analysis did not find a significant relationship (p>0.05) between TaqI polymorphisms with TT and TC genotypes and clinicopathology of breast cancer but found a significant relationship (p=0.047) with histological grading. The TT and TC genotypes were found to increase the risk of breast cancer severity by 3,810 compared to individuals without the TT and TC genotypes. Analysis of confounding variables with clinicopathological breast cancer found no significant relationship (p>0.05) between the age of diagnosis, menstrual status, and hormonal contraceptive use with the characteristics of the sample.\u0000Conclusion: It was concluded that breast cancer patients at Sanglah Hospital Bali had TaqI polymorphisms with TT and TC genotypes. TT and TC genotypes are associated with histological grading and can increase the severity of histological grading in breast cancer patients at Sanglah Hospital Denpasar Bali.","PeriodicalId":55769,"journal":{"name":"Indonesia Journal of Biomedical Science","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80576762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}