Atla-Alternatives To Laboratory Animals最新文献_第8页

In Silico Phototoxicity Prediction of Drugs and Chemicals by using Derek Nexus and QSAR Toolbox. 利用 Derek Nexus 和 QSAR 工具箱对药物和化学品进行硅学光毒性预测。

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-07-01 Epub Date: 2024-06-23 DOI: 10.1177/02611929241256040

Varun Ahuja, Gowrav Adiga Perdur, Zabiullah Aj, Mohan Krishnappa, Helena Kandarova

{"title":"In Silico Phototoxicity Prediction of Drugs and Chemicals by using Derek Nexus and QSAR Toolbox.","authors":"Varun Ahuja, Gowrav Adiga Perdur, Zabiullah Aj, Mohan Krishnappa, Helena Kandarova","doi":"10.1177/02611929241256040","DOIUrl":"10.1177/02611929241256040","url":null,"abstract":"Phototoxicity testing is crucial for evaluating the potential harmful effects of pharmaceuticals and chemicals on human skin when exposed to sunlight. Traditional in vivo models involving mice, rats, guinea pigs, as well as in vitro assays such as the 3T3 Neutral Red Uptake phototoxicity assay and methods based on the use of reconstructed human epidermis, have been established for phototoxicity testing. While these approaches are extremely valuable, they are costly in terms of both time and resources. Consequently, in silico approaches based on the use of predictive software tools can offer more rapid and cost-effective phototoxicity screening solutions. With this goal in mind, the current study evaluated two in silico tools - Derek Nexus 6.1.0/Derek Knowledge Base 2020 1.0 (Lhasa Limited, UK) and the QSAR Toolbox (v 4.5) developed by the Organisation for Economic Co-operation and Development (OECD) - for their capacity to predict the phototoxicity of several substances from diverse classes. Derek Nexus and the QSAR Toolbox were both found to be very useful for predicting the phototoxicity of drugs and other chemicals. Derek Nexus predicted phototoxicity of the compounds, with a sensitivity of 63%, specificity of 93%, Positive Predictive Values of 90% and Negative Predictive Value of 69%, overall accuracy of 77% and balanced accuracy of 78%. The QSAR Toolbox achieved sensitivity of 73%, specificity of 85%, Positive Predictive Value of 85% and Negative Predictive Value of 74%, overall accuracy of 79% and balanced accuracy of 79%. The results show that Derek Nexus and the QSAR Toolbox can be usefully incorporated in the workflow of phototoxicity testing for pharmaceuticals and chemicals.","PeriodicalId":55577,"journal":{"name":"Atla-Alternatives To Laboratory Animals","volume":" ","pages":"195-204"},"PeriodicalIF":2.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141443805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Cigarette Smoke Constituents and Nicotine Differentially Affect Cytokine Production by Human Macrophages Stimulated by TLR Ligands In Vitro: Considerations for a Standardised Protocol. 香烟烟雾成分和尼古丁对体外受 TLR 配体刺激的人类巨噬细胞产生的细胞因子有不同影响：标准化方案的考虑因素。

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-07-01 Epub Date: 2024-06-13 DOI: 10.1177/02611929241259105

Abeer Abdullah M Sharaf, Ian Todd

{"title":"Cigarette Smoke Constituents and Nicotine Differentially Affect Cytokine Production by Human Macrophages Stimulated by TLR Ligands In Vitro: Considerations for a Standardised Protocol.","authors":"Abeer Abdullah M Sharaf, Ian Todd","doi":"10.1177/02611929241259105","DOIUrl":"10.1177/02611929241259105","url":null,"abstract":"Chronic obstructive pulmonary disease (COPD) is an inflammatory lung condition associated with cigarette (tobacco) smoking. Numerous in vivo animal studies have been conducted to investigate the links between cigarette smoke, nicotine and infection in lung pathology. As an alternative to animal experiments, we used an in vitro system to investigate the effects of cigarette smoke extract (CSE) or nicotine on TNF-α and IL-10 production by monocyte-derived human macrophages. The macrophages were simultaneously stimulated with either poly-IC (as a chemical surrogate for viral infection) or lipopolysaccharide (as a chemical surrogate for Gram-negative bacterial infection). CSE enhanced TNF-α production, whereas nicotine inhibited IL-10 production by the macrophages, particularly when co-stimulated with the microbial chemical surrogates. A system of this type may help to further our understanding of the immunological and inflammatory effects of smoking, without recourse to in vivo studies. Requirements for the optimisation and standardisation of such an in vitro system are also discussed.","PeriodicalId":55577,"journal":{"name":"Atla-Alternatives To Laboratory Animals","volume":" ","pages":"205-213"},"PeriodicalIF":2.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141319076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial. 社论

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-07-01 Epub Date: 2024-06-12 DOI: 10.1177/02611929241263184

Judith C Madden

引用次数: 0

Spotlight on Three Rs Progress. 聚焦三个 R 的进展。

IF 2.7 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-06-13 DOI: 10.1177/02611929241260578

引用次数: 0

Conference Diary. 会议日记。

IF 2.7 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-05-01 Epub Date: 2024-04-09 DOI: 10.1177/02611929241245506

引用次数: 0

Editorial. 社论

IF 2.7 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-05-01 Epub Date: 2024-04-15 DOI: 10.1177/02611929241246891

Judith C Madden

引用次数: 0

Chitosan-Calcium Aluminate as a Cell-homing Scaffold: Its Bioactivity Testing in a Microphysiological Dental Pulp Platform. 作为细胞归巢支架的壳聚糖-铝酸钙：在微生理学牙髓平台中的生物活性测试

IF 2.7 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-03-01 Epub Date: 2024-02-13 DOI: 10.1177/02611929241232558

Ester Alves Ferreira Bordini, Vitor de Toledo Stuani, Lígia Espoliar Correa, Fernanda Balestrero Cassiano, Marcella Fernandes Lovison, Maria Luisa Leite, Josimeri Hebling, Carlos Alberto de Souza Costa, Diana Gabriela Soares

{"title":"Chitosan-Calcium Aluminate as a Cell-homing Scaffold: Its Bioactivity Testing in a Microphysiological Dental Pulp Platform.","authors":"Ester Alves Ferreira Bordini, Vitor de Toledo Stuani, Lígia Espoliar Correa, Fernanda Balestrero Cassiano, Marcella Fernandes Lovison, Maria Luisa Leite, Josimeri Hebling, Carlos Alberto de Souza Costa, Diana Gabriela Soares","doi":"10.1177/02611929241232558","DOIUrl":"10.1177/02611929241232558","url":null,"abstract":"In vitro models of the dental pulp microenvironment have been proposed for the assessment of biomaterials, to minimise animal use in operative dentistry. In this study, a scaffold/3-D dental pulp cell culture interface was created in a microchip, under simulated dental pulp pressure, to evaluate the cell-homing potential of a chitosan (CH) scaffold functionalised with calcium aluminate (the 'CHAlCa scaffold'). This microphysiological platform was cultured at a pressure of 15 cm H2O for up to 14 days; cell viability, migration and odontoblastic differentiation were then assessed. The CHAlCa scaffold exhibited intense chemotactic potential, causing cells to migrate from the 3-D culture to its surface, followed by infiltration into the macroporous structure of the scaffold. By contrast, the cells in the presence of the non-functionalised chitosan scaffold showed low cell migration and no cell infiltration. CHAlCa scaffold bioactivity was confirmed in dentin sialophosphoprotein-positive migrating cells, and odontoblastic markers were upregulated in 3-D culture. Finally, in situ mineralised matrix deposition by the cells was confirmed in an Alizarin Red-based assay, in which the CHAlCa and CH scaffolds were adapted to fit within dentin discs. More intense deposition of matrix was observed with the CHAlCa scaffold, as compared to the CH scaffold. In summary, we present an in vitro platform that provides a simple and reproducible model for selecting and developing innovative biomaterials through the assessment of their cell-homing potential. By using this platform, it was shown that the combination of calcium aluminate and chitosan has potential as an inductive biomaterial that can mediate dentin tissue regeneration during cell-homing therapies.","PeriodicalId":55577,"journal":{"name":"Atla-Alternatives To Laboratory Animals","volume":" ","pages":"107-116"},"PeriodicalIF":2.7,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139731125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spotlight on Three Rs Progress. 聚焦三个 R 的进展。

IF 2.7 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-03-01 Epub Date: 2024-02-20 DOI: 10.1177/02611929241232216

引用次数: 0

Resources Round-up. 资源综述。

IF 2.7 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-03-01 Epub Date: 2024-02-13 DOI: 10.1177/02611929241232217

引用次数: 0

A Cytotoxicity Assay as an Alternative to the Murine Model for the Potency Testing of Bothrops jararaca Venom and Antivenom: An Intralaboratory Pre-validation Study. 细胞毒性测定法可替代小鼠模型，用于箭毒双蛛毒液和抗蛇毒血清的效力测试：实验室内预验证研究》。

IF 2.7 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-03-01 Epub Date: 2024-03-04 DOI: 10.1177/02611929241237518

Renata N C Nundes, Antonio E C C Almeida, Wlamir C Moura, Marcelo S Gonzalez, Humberto P Araújo

{"title":"A Cytotoxicity Assay as an Alternative to the Murine Model for the Potency Testing of Bothrops jararaca Venom and Antivenom: An Intralaboratory Pre-validation Study.","authors":"Renata N C Nundes, Antonio E C C Almeida, Wlamir C Moura, Marcelo S Gonzalez, Humberto P Araújo","doi":"10.1177/02611929241237518","DOIUrl":"10.1177/02611929241237518","url":null,"abstract":"Antivenom therapy is the only specific treatment for snakebite envenomation, and antivenom potency determination is key in the efficacy assurance quality control process. Nowadays, this process relies on the in vivo murine model - thus, the development of alternative in vitro methods is imperative. In the current study, the principle of the proposed method is the ability of Bothrops venom to induce cytotoxic effects in Vero cells, and the capacity to evaluate the inhibition of this cytotoxicity by the respective antivenom. After exposure to the venom/antivenom, the relative proportions of adherent (viable) cells were evaluated by direct staining with Coomassie Blue. The optical density (OD) of the lysed cell eluate was directly proportional to the number of adherent cells. This cytotoxicity-based alternative method could represent a potential candidate for validation as a replacement for the current in vivo test. The in vitro-determined cytotoxicity of the Brazilian Bothrops reference venom (expressed as the 50% effective concentration; EC50) was 3.61 μg/ml; the in vitro-determined 50% inhibitory concentration (IC50) of the Brazilian Bothrops reference antivenom was 0.133 μl/ml. From these two values, it was possible to calculate the potency of the reference antivenom. The results from the assays exhibited a good linear response, indicating that the method could be a potential candidate replacement method for use in antivenom quality control prior to lot release, subject to further validation.","PeriodicalId":55577,"journal":{"name":"Atla-Alternatives To Laboratory Animals","volume":" ","pages":"82-93"},"PeriodicalIF":2.7,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140029649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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