Biofuels Bioproducts & Biorefining-Biofpr最新文献

{"title":"An efficient and industrially feasible process for purification and immobilization of orange-peel-based lipase for biocatalytic applications","authors":"Sahil Ghutake,&nbsp;Varunraj Gharge,&nbsp;Hitesh Pawar","doi":"10.1002/bbb.2725","DOIUrl":"https://doi.org/10.1002/bbb.2725","url":null,"abstract":"<p>Lipase is an important enzyme with wide industrial applications in various sectors. The present study focuses on the development of an efficient and industrially feasible process for the purification and immobilization of orange-peel-based lipase. The integration of ultrafiltration (UF), adsorptive chromatography, and immobilization on synthetic resins offers an efficient method for producing purified, immobilized lipases. Ultrafiltration membranes with varying molecular weight cutoffs were evaluated for concentrating crude lipase. Among the membranes tested, the 5 kDa UF membrane demonstrated over 94% enzyme activity retention. Various microporous adsorbents (hydrophilic, hydrophobic and ion exchange) were tested for removing color impurities from concentrated crude lipase. The hydrophobic adsorbent achieved over 90% removal of color components. Overall, the integration of UF membranes and adsorptive chromatography showed a 2.29-fold increase in production efficiency. Different microporous adsorbents were tested for the immobilization of purified lipase to produce an immobilized lipase biocatalyst. The use of hydrophobic adsorbents with lower porosity and a larger surface provides maximum binding efficiency (95%). The characterization study of purified lipase was conducted by using Fourier transform infrared (FTIR) spectroscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The purification process yielded a lipase enzyme concentration of 0.28 mg mL⁻¹ with a specific activity of 250.54 U mL⁻¹, achieving an approximately 2.29-fold increase in purity. This study presents an efficient and industrially viable method for the purification and immobilization of lipases, enabling their use in biocatalytic applications.</p>","PeriodicalId":55380,"journal":{"name":"Biofuels Bioproducts & Biorefining-Biofpr","volume":"19 2","pages":"420-436"},"PeriodicalIF":3.2,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From refuse to resource: exploring technological and economic dimensions of waste-to-energy","authors":"Mohammed Al-Hammadi,&nbsp;Mine Güngörmüşler","doi":"10.1002/bbb.2723","DOIUrl":"https://doi.org/10.1002/bbb.2723","url":null,"abstract":"<p>Waste valorization offers a sustainable approach to waste management and the generation of biofuels and bioenergy, mitigating the environmental impact of fossil fuel production and use. Carbon emissions and food-source competition associated with first-generation biofuels production can be mitigated. Reports on thermochemical and biochemical pathways for generating bio-oil and syngas provide extensive coverage of production processes but lack recent insights into technological advances for upgrading these outputs into biodiesel, biomethanol, bioethanol, biogas, and biomethane. They also often omit information on commercial status, leading and prospective companies, market size, future market predictions, and associated challenges. The drawbacks of waste valorization do not appear to have been discussed widely in the literature – they include deforestation, land-use changes, soil texture changes, and biodiversity and social impacts. To address these gaps in the literature, this review examines the factors mentioned above, focusing on the drawbacks of waste-to-energy conversion and proposing solutions targeted at governments, policymakers, investors, entrepreneurs, and researchers seeking to understand and mitigate the challenges in this sector.</p>","PeriodicalId":55380,"journal":{"name":"Biofuels Bioproducts & Biorefining-Biofpr","volume":"19 2","pages":"570-592"},"PeriodicalIF":3.2,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rethinking the sustainable bioeconomy at a turning point in my life","authors":"Bruce E. Dale","doi":"10.1002/bbb.2707","DOIUrl":"https://doi.org/10.1002/bbb.2707","url":null,"abstract":"","PeriodicalId":55380,"journal":{"name":"Biofuels Bioproducts & Biorefining-Biofpr","volume":"19 1","pages":"7-8"},"PeriodicalIF":3.2,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143110737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An optimal bioenergy system design framework based on the water-energy-agriculture nexus","authors":"Rashi Dhanraj,&nbsp;Yogendra Shastri","doi":"10.1002/bbb.2714","DOIUrl":"https://doi.org/10.1002/bbb.2714","url":null,"abstract":"<p>Scaling biomass-to-ethanol systems in water-stressed regions is challenging, particularly considering the impacts of climate change. This study presents an optimization model that incorporates agricultural cropping patterns as a decision variable alongside the design of lignocellulosic biorefinery systems. The model aims to identify configurations that prevent overexploitation of regional water resources. Building on a basic optimization framework previously developed for India, this work refines and extends the formulation. Key decision variables include district-level cropping patterns for selected crops, as well as the location, size, and biomass collection strategies of biorefineries to produce ethanol. Four different objectives capture the environmental and socio-economic dimensions of the problem. Regional precipitation is taken as the input, and surface and groundwater availability are modeled as a function of precipitation. A novel aspect of this work is the introduction of simplified linear relationships between groundwater recharge and precipitation, as well as between surface runoff and precipitation, considering regional variations in soil type, slope, and crop type. The model is applied to a case study of Maharashtra, India. The minimum ethanol production cost is ₹47/L ($ 0.56/L), and the maximum farmers' profit is approximately ₹33 550/ha ($ 400.11/ha). A 40% reduction in precipitation due to climate change can double the total irrigation water requirements. The Central Maharashtra Plateau is identified as the most important agroclimatic zone in the state. An increased ethanol blending target increases ethanol costs and reduces farmers' profits. The model can be used as a decision support tool to design the bioenergy system while staying within regional water resource sustainability constraints.</p>","PeriodicalId":55380,"journal":{"name":"Biofuels Bioproducts & Biorefining-Biofpr","volume":"19 2","pages":"369-390"},"PeriodicalIF":3.2,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative performance of Fe-MOx/SiO2 (M = V, Mn, Co, Ni, Cu, Mo) and FeOx/SiO2 catalysts in reductive liquefaction of switchgrass in ethanol","authors":"Ananda S. Amarasekara,&nbsp;Gabriel Murillo Morales,&nbsp;Raghava R. Kommalapati","doi":"10.1002/bbb.2720","DOIUrl":"https://doi.org/10.1002/bbb.2720","url":null,"abstract":"<p>The catalytic hydrothermal liquefaction of biomass under a hydrogen atmosphere is a promising technology to produce stable biocrude oil as a sustainable alternative to petroleum crude. A series of iron-based non-noble mix metal-oxide-on-silica catalysts were evaluated to mimic the natural transformation that may have led to the conversion of terrestrial biomass to fossilized fuels. Switchgrass powder was liquefied to a stable bio-oil with a 71.2% yield by using FeO_x/SiO₂ catalyst in ethanol under a 5.5 MPa hydrogen atmosphere at 210 °C. The use of Fe-MO_x/SiO₂ (M = V, Mn, Co, Ni, Cu and Mo) type bimetallic oxide catalysts instead of FeO_x/SiO₂ can produce improvements in liquefaction yields by using Mn, Co, Ni, and Cu as the second metal. The highest liquefaction yield of 78.8% was observed with the Fe-CuO_x/SiO₂ catalyst. Liquefaction oils were formed that were composed of complex mixtures of C6-C12 alcohols, esters, aldehydes, and phenols. The lignin products:holocellulose products ratio changed in the range 0.35 to 0.15 and the composition of oils changed significantly with the use of mixed metal oxides in place of single metal FeO_x/SiO₂ The most effective catalyst, Fe-CuO_x/SiO₂ could be reused in five cycles with a small loss in liquefaction yield from 78.8% to 70.0% after four reuse cycles and after regeneration of the catalyst at 500 °C for 3 h in air.</p>","PeriodicalId":55380,"journal":{"name":"Biofuels Bioproducts & Biorefining-Biofpr","volume":"19 2","pages":"409-419"},"PeriodicalIF":3.2,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maximizing yields of furfural and 5-hydroxymethylfurfural in side streams from steam explosion of lignocellulosic residues","authors":"Dag Helge Hermundsgård,&nbsp;Solmaz Ghoreishi,&nbsp;Mihaela Tanase-Opedal,&nbsp;Størker T. Moe,&nbsp;Rune Brusletto,&nbsp;Tanja Barth","doi":"10.1002/bbb.2719","DOIUrl":"https://doi.org/10.1002/bbb.2719","url":null,"abstract":"<p>The demand for renewable energy and chemicals from biomass resources has led to the development of methods for biorefining lignocellulosic materials into pellets and value-added chemicals. This has, in turn, driven a search for alternative raw materials to use as feedstock in such biorefineries. This study proposes the inclusion of low-grade lignocellulosic residues from agriculture and forestry, such as straw and woody biomass with a high bark content, in the feedstock mix for steam explosion (STEX) based biorefineries. It focuses on exploring how the use of low-grade residues affects the yield of value-added chemicals that arise as side streams during the steam explosion of lignocellulosic biomass and if acetic acid impregnation of the biomass prior to STEX pretreatment will further improve yields of valuable side stream chemicals. The raw materials were impregnated with water/acetic acid before being subjected to STEX pretreatment, using high temperatures (190 °C–223 °C), and a reaction time of 8 min before pressure release. Value-added chemicals were identified in two separate aqueous side streams. In the condensed process effluents, the most abundant compounds were acetic acid (1.2 g*kg⁻¹ dry input biomass) and furfural (1.4 g*kg⁻¹ dry input biomass), and the highest yields were found in the samples from mixed spruce and branches and tops (BRAT) at high temperatures. Filtrate samples produced from washing the steam-exploded biomass contained acetic acid (53.6 g*kg⁻¹ dry input biomass), furfural (4.1–7.9 g*kg⁻¹ dry input biomass) and 5-hydroxymethylfurfural (5-HMF) (9.0 g*kg⁻¹ dry input biomass) as the most abundant compounds and highest yields of target compounds were found in samples of spruce alone and of spruce mixed with BRAT. All compounds in the side streams were identified and quantified using quantitative ¹H nuclear magnetic resonance spectroscopy (qNMR). This study demonstrates that high yields of valuable chemicals are present in the aqueous side streams of pellet production using alternative feedstocks such as forestry and agricultural residues.</p>","PeriodicalId":55380,"journal":{"name":"Biofuels Bioproducts & Biorefining-Biofpr","volume":"19 2","pages":"319-336"},"PeriodicalIF":3.2,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bbb.2719","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Review of chemical characterization methods and data for compositional analysis of fruit wastes: current status and opportunities","authors":"Daniel D. Durán-Aranguren,&nbsp;John A. Posada,&nbsp;Rocío Sierra,&nbsp;Solange I. Mussatto","doi":"10.1002/bbb.2715","DOIUrl":"https://doi.org/10.1002/bbb.2715","url":null,"abstract":"<p>Fruit waste (FW), mainly from agroindustry, is currently left behind in landfills despite its rich composition. The bioactive compounds (e.g., oils, polyphenols), carbohydrates, and lignin present in this biomass type require comprehensive characterization (i.e., identification and quantification) before they can be used as raw materials in biorefineries. This review collected information from scientific papers on FW compositional analysis methods and characterization data; the information needs to be compiled in a systematic, standardized, and comprehensive way to understand and quantify the true potential of FW as feedstocks for biorefineries. The information gathered in this review allowed us to identify the biomass fractions that could be valorized further depending on the kind of FW (peels, seeds, or seed vessels, and pomace or mixed residues). Fruit waste differs from conventional lignocellulosic biomass due to the presence of higher amounts (&gt;5%) of extractives – pectin, and starch. This review describes current compositional analysis methodologies to identify possible strengths and weaknesses that could affect the adequate selection of valorization platforms. As no current methodology allows the composition of FW to be described thoroughly, this work identifies procedures applicable to biorefineries that use FW. Possible improvements are suggested to fill methodological gaps in the quantification of samples with large amounts of extractives and pectin. The standardization of methods for FW's quantification is fundamental for the adequate integration of different valorization platforms into biorefineries. It is essential to consider all the substances present in FW to exploit fully their potential for new value-added molecules, including oils, polyphenols, and pectin.</p>","PeriodicalId":55380,"journal":{"name":"Biofuels Bioproducts & Biorefining-Biofpr","volume":"19 2","pages":"508-539"},"PeriodicalIF":3.2,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Examination of the effect of temperature, biomass characteristics, and heating rates on volatile release yield in palm kernel shell pyrolysis using volatile state kinetic modeling","authors":"Pandit Hernowo,&nbsp;Soen Steven,&nbsp;Amalia Syauket,&nbsp;Dede Rukmayadi,&nbsp;Anton Irawan,&nbsp;Carolus B. Rasrendra,&nbsp;Yazid Bindar,&nbsp;Ibnu Maulana Hidayatullah,&nbsp;Intan Clarissa Sophiana,&nbsp;Rita Dwi Ratnani,&nbsp;Komang Ria Saraswati","doi":"10.1002/bbb.2717","DOIUrl":"https://doi.org/10.1002/bbb.2717","url":null,"abstract":"<p>The behavior of biomass pyrolysis can be predicted by analyzing its characteristics. This study aimed to model the release of volatiles across various temperatures, biomass properties, and heating rates. Palm kernel shells were pyrolyzed at 433–773 K with a heating rate of 5 K·min⁻¹ using volatile-state kinetic modeling. The process began by calculating the biomass type number (<i>N</i>_<i>CT</i>), which was used to determine volatile enhancement (<i>V</i>_<i>E</i>), volatile release yield (<i>Y</i>_<i>VY</i>), product yield (<i>Y</i>_<i>i</i>), and product mass fraction (<i>y</i>_<i>i</i>). The kinetic parameters, including the activation energy for product formation (<i>E</i>_<i>ai</i>), were derived through a fitting process.</p><p>The results indicate a <i>Y</i>_<i>VY</i> of 70.77% within the devolatilization zone, corresponding to the degradation of cellulose and hemicellulose. The <i>Y</i>_<i>VY</i> increased with higher temperatures, lower <i>N</i>_<i>CT</i>, and higher heating rates. The activation energy ranged from 155–185 kJ·mol⁻¹ for biocrude oil (BCO) and 149–186 kJ·mol⁻¹ for gas. The kinetic parameters from the volatile-state kinetic model demonstrated errors below 0.2% in comparison with the experimental data, confirming the model's accuracy and reliability.</p>","PeriodicalId":55380,"journal":{"name":"Biofuels Bioproducts & Biorefining-Biofpr","volume":"19 2","pages":"391-408"},"PeriodicalIF":3.2,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of Ag/ZnO catalysts using cassava starch applied to the degradation of emerging contaminants","authors":"Joel Miranda do Nascimento,&nbsp;Fernado Manzotti de Souza,&nbsp;Giane Gonçalves Lenzi,&nbsp;Angelo Marcelo Tusset,&nbsp;Giovanna Gonçalves,&nbsp;Leda Saragiotto Colpini,&nbsp;Rodrigo Brackmann,&nbsp;Onelia Aparecida Andreo dos Santos","doi":"10.1002/bbb.2712","DOIUrl":"https://doi.org/10.1002/bbb.2712","url":null,"abstract":"<p>This work describes a new synthetic route for the production of a low-cost and environmentally friendly catalyst: a zinc oxide-based system doped with silver, prepared using a novel sol–gel method with cassava starch to form the gel. The photocatalysts were characterized by different techniques: X-ray diffraction (XRD), X-ray fluorescence spectroscopy (XRF), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), photoacoustic spectroscopy (PAS), and point of zero charge (PZC). The XRF and EDS results indicated that photocatalysts doped with 2%, 5%, 8%, and 10%, silver could be obtained successfully. The synthesis method employed produced nanoparticles of ~30 nm, as confirmed by XRD and TEM results. The band gap energy values of the materials (ranging from 1.98 to 3.05 eV) fall within the range suitable for catalyst activation under UV radiation. Photocatalytic reactions were carried out with two emerging pollutants, 2,4-<span>d</span>-dichlorophenoxyacetic acid (2,4D) and caffeine, to verify the catalytic activity of the synthesized catalyst. The results indicated that the catalyst was efficient for the degradation of both pollutants. Tests using solar radiation led to 100% degradation of 2,4D. Cl⁻ ions did not influence the process negatively. The amount of caffeine removed by the photocatalytic process used in solar radiation was ~97% due to the low band gap energy values of the synthesized material.</p>","PeriodicalId":55380,"journal":{"name":"Biofuels Bioproducts & Biorefining-Biofpr","volume":"19 2","pages":"305-318"},"PeriodicalIF":3.2,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Techno-economic and environmental assessment of converting mixed prairie to renewable natural gas with co-product hydroxycinnamic acid","authors":"Katherine Wild,&nbsp;Elmin Rahic,&nbsp;Lisa Schulte,&nbsp;Mark Mba Wright","doi":"10.1002/bbb.2710","DOIUrl":"https://doi.org/10.1002/bbb.2710","url":null,"abstract":"<p>There is a need for research into clean alternative energy sources to meet future energy requirements. Renewable natural gas (RNG) can assist in fulfilling a growing projected demand for natural gas in a sustainable manner. This study focuses on a techno-economic analysis (TEA) and a life cycle assessment (LCA) for the co-digestion of manure with pretreated prairie biomass. This process offers higher biogas yields and an additional co-product, hydroxycinnamic acid (HCA), to decrease the minimum fuel selling price (MFSP) of RNG. The plant produces between 524 000 GJ year⁻¹ and 1 176 000 GJ year⁻¹ of RNG in the five modeled scenarios. The MFSP of RNG for scenarios where biomass pretreatment occurs is estimated to be between $15.87 GJ⁻¹ and $18.94 GJ⁻¹. The MFSP for the scenario where no pretreatment occurs is estimated to be $21.21 GJ⁻¹. The global warming potential results range from −7.30 kg CO₂e GJ⁻¹ to 21.59 kg CO₂e GJ⁻¹. Sensitivity and uncertainty analyses are also completed.</p>","PeriodicalId":55380,"journal":{"name":"Biofuels Bioproducts & Biorefining-Biofpr","volume":"19 2","pages":"288-304"},"PeriodicalIF":3.2,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bbb.2710","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}