Biology Bulletin最新文献

{"title":"Melissa officinalis Interaction with Opioid Receptors Attenuates Stress-Induced Anxiety-Like and OCD-Like Behaviors","authors":"M. Eivani, S. Gholipour Miarkolae, J. Ebrahimian, F. Karimani, S. M. Khoshnazar","doi":"10.1134/s1062359023606195","DOIUrl":"https://doi.org/10.1134/s1062359023606195","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p><i>Melissa officinalis</i> (MO) is a valuable medicinal herb from the Lamiaceae family, MO extracts possess different beneficial effects, including anti-inflammatory, antioxidant, memory enhancer, anxiolytic, and anti-depression. It seems that phytochemicals found in MO including flavonols (luteolin and apigenin) and polyphenols (rosmarinic acid and caffeic acid) are responsible for the beneficial effects of MO. The present study aimed to assess the role of opioid receptors in the effect of MO in anxiety and obsessive-compulsive disorder (OCD) in stressed rats. Male hooded rats received oral administration of MO (50, 100 and 200 mg/kg), with or without intraperitoneal injection of naloxone (1 mg/kg), and were exposed to 30 min stress, then anxiety-like and OCD-like behaviors were evaluated using light/dark box and marble burying test. Our results showed that oral administration of MO has anxiolytic effects and a reduction in OCD-like behavior in stressed rats that could be inhibited by injection of naloxone. On the other hand, <i>in silico</i> study showed that phytochemicals found in MO have a possible affinity for opioid receptors. Taken together, these data revealed that the opioid receptors may be involved in the anxiolytic effect of MO in stressed rats.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"88 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141195865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reveal of Gut Microbiota of Nymphs Ameletus inopinatus (Eaton), 1887 (Ephemeroptera: Ameletidae) with Molecular and Microbiological Analyzes","authors":"N. Ertorun, M. B. Mutlu","doi":"10.1134/s1062359024606554","DOIUrl":"https://doi.org/10.1134/s1062359024606554","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Mayflies, recognized for their hemimetabolous life cycle and ephemeral adult phase, exhibit diverse larval forms categorized as burrowing, flattened, swimming, and creeping. <i>Ameletus inopinatus</i> Eaton, 1887, a member of the Ameletidae family within the order Ephemeroptera, undergoes aquatic development and is commonly found in the tranquil stretches of small rivers or streams, particularly among stones and boulders. This study focused on <i>A. inopinatus</i> nymphs collected from Yarımca village in Eskişehir, Turkiye. Employing molecular techniques and culture-independent methods, rRNA-based analyses were conducted to characterize the archaeal and bacterial communities within the nymphs’ gut. Microbial DNA was extracted from the gut, and PCR amplification targeted 16S rRNA genes for both Archaea and Bacteria domains. Denaturing gradient gel electrophoresis (DGGE) separated the amplified products, yielding six distinct bands with Bacteria-specific primers. The subsequent analysis of 16S rRNA sequencing from DGGE bands revealed a predominance of the Proteobacteria phylum within the gut lumen. Complementary investigations into prokaryotic diversity employed fluorescence in situ hybridization (FISH) and 4',6-diamidino-2-phenylindole (DAPI) staining. Total DAPI cell counts indicated a microbial cell population of approximately 10⁶ ± 0.18 in the samples. The overarching conclusion derived from these findings is that the microbial community within the <i>Ameletus inopinatus</i> gut is predominantly associated with the Bacteria domain. Recognizing the ecological importance of this symbiotic relationship, the coexistence of mayflies with specific microbial communities is emphasized, underscoring the potential mutualistic roles played by these microorganisms in the life cycle and ecological dynamics of <i>Ameletus inopinatus</i>. This research not only contributes valuable insights into the microbial ecology of mayfly nymphs but also underscores the significance of understanding the intricate relationships between insects and their associated microorganisms for broader ecological perspectives and potential applications in environmental management.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"31 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141195643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testicular Morphologies of Two Sympatric Podarcis Species","authors":"B. Baycan, D. Kurtul, B. Boran, C. N. Özgül, Ç. Gül, M. Tosunoğlu","doi":"10.1134/s106235902360592x","DOIUrl":"https://doi.org/10.1134/s106235902360592x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The reproductive strategies of reptiles vary according to many factors. In lizards, intraspecific and interspecific competition, and environmental and seasonal factors affect their reproductive biology. This study aimed to conduct a comparative analysis of testis sizes in the sympatrically coexisting species, <i>Podarcis muralis</i> and <i>Podarcis tauricus</i>, located in Kofçaz, Kırklareli, Turkey, considering both intra-species and inter-species variations. Changes in body sizes and testis sizes according to months were monitored. As a result, it was determined that in both species, there was a positive correlation between body size and testis size, and that in <i>P. tauricus</i>, both body and testis sizes were higher compared to <i>P. muralis</i>, showing a significant difference. It was also observed that in the <i>P. muralis</i> species, the testis size reached its peak value in April (mean daily temperature 9.9°C), while in the <i>P. tauricus</i> species, it reached the highest value in May (mean daily temperature 15.5°C). It was found that the testis size in <i>P. muralis</i> reached its lowest value in August (mean daily temperature 23.8°C), while in <i>P. tauricus</i>, it was lowest in September (mean daily temperature 20.2°C). The fact that testis size is high in different months in these two sympatric species suggests that their active breeding periods differ.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"26 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141195645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Record of Land Hermit Crabs Coenobita pseudorugosus Nakasone, 1988 (Crustacea: Decapoda: Anomura: Coenobitidae) from Taiwan","authors":"Tzu-Pi Chen, Chia-Hsuan Hsu, Yuan-Mou Chang, Sin-Tung Choi, Shi-Sheng Liu","doi":"10.1134/s1062359023605992","DOIUrl":"https://doi.org/10.1134/s1062359023605992","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Land hermit crabs play a pivotal role in ecosystems as scavengers, contributing to the nutrient cycle in coastal forests and serving as a food source for various consumers. Nevertheless, they are subjected to large-scale capture for the pet trade in multiple regions. It is imperative to expand our knowledge of land hermit crabs to enhance conservation efforts. Taiwan is located in the transition zone between tropical and subtropical regions with rich biodiversity. Seven land hermit crab (Family: Coenobitidae) species have been identified here, namely <i>Birgus latro</i>, <i>Coenobita brevimanus</i>, <i>C. cavipes</i>, <i>C. perlatus</i>, <i>C. purpureus</i>, <i>C. rugosus</i>, and <i>C. violascens</i>. This article introduces the scientific record of the 8th species, <i>Coenobita pseudorugosus</i>, in Taiwan. Further ecological research of <i>C. pseudorugosus</i> is essential to deepen our understanding of this species and ensure its conservation status.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"7 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141195726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taxonomic Studies on the Genus Astragalus (Fabaceae) from Mongolia (I): Two New Records of A. testiculatus and A. teskhemicus Based on Morphological and Molecular Evidence","authors":"S. Baasanmunkh, D. Munkhtulga, N. Nyamgerel, B. Oyuntsetseg, A. Undruul, Z. Tsegmed, M. S. Knyazev, H. J. Choi","doi":"10.1134/s1062359023605748","DOIUrl":"https://doi.org/10.1134/s1062359023605748","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p><i>Astragalus</i> L. is one of the largest genera, including over 3000 taxa worldwide. In the present study, we found two new records of <i>A. teskhemicus</i> Sytin and Shaulo and <i>A. testiculatus</i> Pall. in western Mongolia. We provided a morphological revision and DNA barcoding (ITS) of two new records along with that of the most similar <i>Astragalus</i> species including <i>A. ammodytes</i> Pall., <i>A.</i> <i>gubanovii</i> N. Ulziykh., <i>A. hypogaeus</i> Ledeb., <i>A. junatovii</i> Sanchir. Furthermore, we made detailed photo illustrations and wild photos for new records and four species based on our field observations, respectively. The general distribution, taxonomic notes, type information, habitats, and species examined are given.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"26 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141195811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer and Cytotoxic Activities of Some Trigonella Species on MCF-7, L929 and HeLa Cell Lines","authors":"Shahrokh Mojarradgandoukmolla, Hasan Akan, Takım Kasım, Sevgi Durna Dastan, Hatice Gumushan Aktas","doi":"10.1134/s1062359023603567","DOIUrl":"https://doi.org/10.1134/s1062359023603567","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p><i>Trigonella filipes</i> Boiss, <i>Trigonella strangulata</i> Boiss., and <i>Trigonella uninata</i> Banks and Sol., are three commonly used species that grow well in Iraq’s northern suburbs and are frequently consumed and used in traditional medicine. The current study was planned to assess and identify the active chemicals in these plant species, as well as to analyze their anticancer and antiprofeliration effects in rats treated with alcohol and cholesterol. Plant extracts were tested for oxidative stress index (OSI), total oxidant status (TOS), and total antioxidant status (TAS). Administration of <i>Trigonella foenum-graecum</i> and <i>Trigonella monantha</i> significantly improved these changes in diseased rats. MTT cytotoxicity test was used to investigate the cytotoxic properties of <i>Trigonella filipes</i>, <i>Trigonella strangulata,</i> and <i>Trigonella uncinata</i> plant extracts on three distinct cell lines in varied concentrations (1–10–100–1000 g/mL) after 24 h of incubation. The results were supported that these three plant taxa have high phytochemical compounds that have physiological effects such as inhibit cell proliferation, improving the function of liver and kidney function, and antioxidants effect.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"55 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141195650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Defense Response Study in Oilseed Rape (Brassica napus L.) in Response to Beet Armyworm Spodoptera exigua","authors":"Mubasher Ahmad Malik, Ishita Ahuja, Samina Nazeer Ahmad, Atle Bone, Jam Nazeer Ahmad","doi":"10.1134/s1062359023606067","DOIUrl":"https://doi.org/10.1134/s1062359023606067","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>In the Brassicaceae family, a glucosinolate-myrosinase-based system exists against herbivory Glucosinolate-Myrosinase system is differentially compartmentalized but substrate glucosinolate enter in contact with the enzyme myrosinase when plant tissues are attacked by insects. The glucosinolate breakdown into a variety of biologically active toxins further deterrenting the herbivory of different chewing and sucking insect pests. The myrosinase enzyme present is in myrosin cells were genetically removed from <i>B. napus</i> plant during the seed development. These genetically modified myrosin cells free plants are known as <i>MINELESS</i> plant. Genetically modified oilseed rape (<i>Brassica napus</i>) was produced by removing toxic mines. Here, we evaluauted the tritrophic interaction between <i>Brassica napus</i> wildtype and genetically modified and polyphagous pest <i>Spodoptera</i>. In force feeding experiment, larval growth of <i>S. exigua</i> was slow and gained less weight on <i>MINELESS</i> seedlings as compared to wild-type seedling. Additionally, highest amount of total glucosinolate was found in <i>MINELESS</i> control than that of wild type control. Myrosinase activity was not affected by the <i>S. exigua</i> feeding on the <i>MINELESS</i> plants. However, reduced activity of myrosinase was observed in the wild-type seedlings. Moreover, the <i>S. exigua</i> induced the concentration of tryptophan derived glucosinolate (indol-3-yl-methyl, 1-methoxy-indol-3-yl-methyl) that was found slightly higher in <i>MINELESS</i> seedlings than on wild-type seedling. Feeding of <i>S. exigua</i> deregulated the level of glucosinolate hydrolysis products in both types of plants. Transcriptional data after <i>S. exigua</i> feeding on wildtype and <i>MINELESS</i> showed differential response of methyl jasmonate and glucosinolate responsive genes were observed. We also observed that after methyl jasmonate treatment, methyl jasmonate and glucosinolate responsive genes were incease in the wild-type and <i>MINELESS</i> seedlings showing their negative effect on the growth of <i>S. exigua</i>. Overall, generalist insect results are interesting by considering the function of myrosin cells and binary defense system. Further, same experiment should be performed against generalist and specialist insect herbivores of above- and below-ground.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"120 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141195943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Silico Analysis of Phylogeny, Structure, and Function of Flavohemoproteins from Metagenomic Data","authors":"Jaspreet Kaur","doi":"10.1134/s1062359023604391","DOIUrl":"https://doi.org/10.1134/s1062359023604391","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>In this study, flavohemoproteins from protist, bacterial and fungal sources as well those available in metagenome data have been characterized using standard bioinformatics tools in order to study their phylogenetic relationships, three-dimensional structure and functional parameters. 5 clusters of metagenomic proteins were identified which showed the closest neighbors with known genera. The average length of flavohemoprotein in the data set was found to be 414 residues. The average pI and molecular weight (MW) was 6.72 and 47.28 kDa respectively in all strains including metagenome sequences. Flavohemoproteins showed similar amino acid composition among different organisms including proteins from metagenomic data and from known genera, with relative higher abundance of amino acids Ala and Leu. The secondary structure prediction results revealed that the enzymes are dominated by α-helices and random coils. Tertiary structure analysis revealed the presence of three ligand binding sites for Heme, FAD and NAD. The computational analysis of flavohemoproteins from metagenomic data done in this study can provide useful sequence and structural information which can be further used for metaproteomic analysis in search for novel flavohemoprotein enzymes from the environment.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"32 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141195785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Polymorphism of GH and IGF-1 Genes and Body Measurements Traits in Maghrabi Camel","authors":"A. M. Darwish, M. A. Abdelhafez, S. I. Othman, H. A. El-Metwaly, H. A. Rudayni, A. A. Allam","doi":"10.1134/s1062359024606712","DOIUrl":"https://doi.org/10.1134/s1062359024606712","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Nonetheless the key roles of growth hormone (GH) and insulin growth factor-1 (IGF-1) genes in livestock growth traits (including body measurements), few studies deployed them in camels, unlike other livestock species (cattle, buffalo, sheep, and goat). So, this study was performed to investigate the genetic polymorphism of GH and IGF-1 markers, in Maghrabi camels by PCR-SSCP and DNA sequencing tools, with assessing the impacted live body measurements traits. In this study, 65 blood samples were collected from Maghrabi camel, and different body measurements were valued. Different genotypes of GH and IGF-1 genes were determined, via SSCP-PCR and sequencing tools. The results pointed out one genotype for the IGF-I gene, and three genotypes for the GH gene, with 23.21, 42.85, and 33.95 frequencies, of AA, AB, and BB genotypes, respectively. The sequence analysis showed specific single nucleotide polymorphisms (SNPs), for both A and B alleles. Those SNPs triggered alteration in some of the amino acids. Moreover, individuals of the BB genotype recorded the highest value in body weight, Neck length, Height at lumbar area, Anterior limb length, Neck circumference, and increased Body length associated with the AA genotype. Nevertheless, there were no significant differences, between the three genotypes, for the other body measurements. This study found that Maghrabi camels possess three genotypes of the GH gene and one genotype of the IGFI gene, and the BB genotype of the GH gene was associated with high performance of growth traits. Therefore, it is useful to use camel individuals, with the BB genotype of the GH gene, in camelid breeding programs, to improve live body measurement traits.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"44 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141195810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptide Isolated from Leptospermum flavens Sm. Inhibits Human Glutathione Transferases π (hGSTP) Activity and Enhances the Cytotoxicity of Doxorubicin towards HT-29 Cell Line","authors":"W. M. Phang, Z. Alias, N. Aminudin","doi":"10.1134/s1062359023605001","DOIUrl":"https://doi.org/10.1134/s1062359023605001","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Glutathione transferase Pi (GST-P) reportedly overexpressed markedly in cancer cell lines. It was correlated to the resistance towards drugs used in chemotherapy treatment. The study demonstrated the isolation of peptide as potential inhibitor to the enzyme. Glutathione transferase Pi (GST-P) was purified from human colon adenocarcinoma HT-29 cell line using glutathione (GSH)-affinity chromatography. Active components presence in ethanolic extract (50% ethanol) of leaves <i>Leptospermum flavescens</i> Sm. was shown possessing inhibitory property (IC₅₀ of 0.088 mg/mL) towards GST-P in vitro. Further fractionation using polyamide the 50% methanol in 2% acetic acid eluate possessed an inhibitory property at IC₅₀ of 0.191 mg/mL. In the study the IC₅₀ values of doxorubicin are 0.788 and 0.816 μg/mL on HT-29 and MRC-5 cell lines respectively while IC₅₀ values of cisplatin were at 9.49 and 4.07 μg/mL on HT-29 and MRC-5 cell lines respectively. The 50% methanol eluate has significantly non-toxic to both of the cell lines with 100% cell viability at more than 100 μg/mL sample applied. In combination with doxorubicin, 50% methanol eluate enhanced cytotoxicity of the drug towards HT-29 by reduction of IC₅₀ value significantly to 66%. The eluate however only reduced the IC₅₀ to 11% when combined with cisplatin. The study indicates that the eluate can potentiate cytotoxicity of doxorubicin on HT-29 cell line and this effect is correlated to the ability of the eluate to inhibit GST-P in vitro. The purified active molecule was a peptide with molecular weight of 3.5 kDa.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"50 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141195641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}