Biomedical Papers-Olomouc最新文献_第10页

Ascorbic acid intake during pregnancy. 孕期抗坏血酸摄入量。

IF 0.9 4区医学

Biomedical Papers-Olomouc Pub Date : 2023-09-01 Epub Date: 2023-09-06 DOI: 10.5507/bp.2023.035

Kristina Podolska, Dana Mazankova, Maria Goboova, Ivan Vano

引用次数: 0

Real-world outcomes of mepolizumab treatment in severe eosinophilic asthma patients - retrospective cohort study in Slovakia. 美波珠单抗治疗严重嗜酸性粒细胞性哮喘患者的真实结果——斯洛伐克的回顾性队列研究。

IF 0.9 4区医学

Biomedical Papers-Olomouc Pub Date : 2023-09-01 Epub Date: 2023-07-10 DOI: 10.5507/bp.2023.029

Milos Jesenak, Vaclav Vanecek, Martina Ondrusova, Veronika Urdova, Katarina Dostalova, Ludek Hochmuth

{"title":"Real-world outcomes of mepolizumab treatment in severe eosinophilic asthma patients - retrospective cohort study in Slovakia.","authors":"Milos Jesenak, Vaclav Vanecek, Martina Ondrusova, Veronika Urdova, Katarina Dostalova, Ludek Hochmuth","doi":"10.5507/bp.2023.029","DOIUrl":"10.5507/bp.2023.029","url":null,"abstract":"Aims: Mepolizumab, a fully-humanized recombinant IgG1 kappa monoclonal antibody directed against IL-5, has shown improved asthma control and lung function in randomised controlled trials. The aim of this study was to evaluate real-world clinical experience in patients with severe eosinophilic asthma treated with mepolizumab in Slovakia.Methods: A retrospective, non-interventional study based on medical records of all adult asthma patients initiating mepolizumab between November 1, 2017 and January 31, 2019, completing 12 months of treatment. At baseline, general and clinical profile data were recorded 12 months prior to treatment. Primary and secondary endpoints described the results of mepolizumab use at 2, 6, and 12 months after the initiation and compared to baseline. Statistical testing of individual change (in each patient) in selected parameters was performed.Results: The cohort included 17 patients with particularly severe asthma at baseline, with frequent severe exacerbations (SE, median 5 [IQR 4-6]/patient/year), high blood eosinophil counts (median 0.6x109/L), frequent oral corticosteroid (OCS) dependence (82.35%), median dose 15 (IQR 7.5-20) mg/day, impaired lung function, and a spectrum of comorbidities. In a one-year follow-up, the data showed reductions in median SE (0 [IQR 0-1] patient/year, eosinophilia (median 0.175x109/L) and OCS maintenance dose (median 6.25 [IQR 2.5-20] mg/day), all statistically significant after 12 months on mepolizumab. Improved and stabilised lung functions throughout the cohort and a reduced incidence of nasal polyposis were observed.Conclusions: The results provide clinical evidence of mepolizumab efficacy in a real sample of patients with severe asthma when administered in routine care settings in Slovakia.","PeriodicalId":55363,"journal":{"name":"Biomedical Papers-Olomouc","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9776719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Trends in management of ocular syphilis in tertiary uveitis centre in Prague, Czech Republic. 捷克共和国布拉格第三期葡萄膜炎中心眼梅毒治疗趋势。

IF 0.9 4区医学

Biomedical Papers-Olomouc Pub Date : 2023-09-01 Epub Date: 2022-09-15 DOI: 10.5507/bp.2022.038

Aneta Klimova, Michaela Brichova, Jarmila Heissigerova, Eva Rihova, Petra Svozilkova

{"title":"Trends in management of ocular syphilis in tertiary uveitis centre in Prague, Czech Republic.","authors":"Aneta Klimova, Michaela Brichova, Jarmila Heissigerova, Eva Rihova, Petra Svozilkova","doi":"10.5507/bp.2022.038","DOIUrl":"10.5507/bp.2022.038","url":null,"abstract":"Aims: To analyse the hallmarks of ocular manifestations of and therapeutic modalities for syphilis in the last two decades. The clinical features of syphilitic uveitis, and association with the human immunodeficiency virus (HIV) coinfection are described.Methods: Retrospective study of 16 patients diagnosed with ocular syphilis confirmed by serological tests in the General University Hospital in Prague between the years 2004 and 2021. General characteristics of ocular and systemic manifestations and visual functions were analysed.Results: An increasing incidence of syphilitic uveitis correlates with a general rise in syphilis cases. In our study, the ocular manifestation of syphilis was panuveitis (44%), posterior uveitis (31%) and anterior uveitis (25%). Posterior uveitis was found in 3 patients (19%) associated with preretinal infiltrates, that are often present in syphilitic uveitis. The worst visual outcomes were among patients with human immunodeficiency virus (HIV) coinfection and/or neurosyphilis, however the data were not significant. Optic disc edema was present in 56%, macular involvement in 37% of patients. Overall, 31% of patients in our cohort had persistent visual field defects due to impairment of their optic nerve or macula despite the final median Snellen visual acuity of 1.0. Two out of sixteen patients were treated with corticosteroids in addition to antibiotics.Conclusion: Posterior uveitis with preretinal infiltrates and optic disc edema should arouse suspicion of ocular syphilis. Recent data show the advantages of adjacent systemic corticosteroid treatment for severe forms of syphilitic uveitis and/or neuritis. Our observation supports this finding.","PeriodicalId":55363,"journal":{"name":"Biomedical Papers-Olomouc","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40371846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Impaired intestinal permeability in patients with multiple sclerosis. 多发性硬化症患者肠通透性受损。

IF 0.9 4区医学

Biomedical Papers-Olomouc Pub Date : 2023-08-11 DOI: 10.5507/bp.2023.033

Lenka Fialova, Pavla Barilly, Ivana Stetkarova, Ales Bartos, Libuse Noskova, Denisa Zimova, Michal Zido, Iva Hoffmanova

{"title":"Impaired intestinal permeability in patients with multiple sclerosis.","authors":"Lenka Fialova, Pavla Barilly, Ivana Stetkarova, Ales Bartos, Libuse Noskova, Denisa Zimova, Michal Zido, Iva Hoffmanova","doi":"10.5507/bp.2023.033","DOIUrl":"https://doi.org/10.5507/bp.2023.033","url":null,"abstract":"Background: A number of recent studies have shown that the intestinal microbiome, part of the brain-gut axis, is implicated in the pathophysiology of multiple sclerosis. An essential part of this axis, is the intestinal barrier and gastrointestinal disorders with intestinal barrier dysregulation appear to be linked to CNS demyelination, and hence involved in the etiopathogenesis of multiple sclerosis (MS).Objective: The aim of this study was to evaluate the integrity of the intestinal barrier in patients with clinically definite multiple sclerosis (CDMS) and clinically isolated syndrome (CIS) using two serum biomarkers, claudin-3 (CLDN3), a component of tight epithelial junctions, and intestinal fatty acid binding protein (I-FABP), a cytosolic protein in enterocytes.Methods: Serum levels of CLDN3 in 37 MS patients and 22 controls, and serum levels of I-FABP in 46 MS patients and 51 controls were measured using commercial ELISA kits. Complete laboratory tests excluded the presence of gluten-related disorders in all subjects. Thirty MS patients received either disease-modifying drugs (DMD), immunosuppression (IS) or corticosteroid treatment.Results: CLDN3 levels were only significantly higher in the MS patients treated with DMD or IS compared to the control group (P=0.006). There were no differences in I-FABP serum levels between the groups. Serum CLDN3 levels did not correlate with serum I-FABP levels in CDMS, in CIS patients or controls.Conclusions: In multiple sclerosis patients, the intestinal epithelium may be impaired with increased permeability, but without significant enterocyte damage characterized by intracellular protein leakage. Based on our data, CLDN3 serum levels appear to assess intestinal dysfunction in MS patients but mainly in treated ones.","PeriodicalId":55363,"journal":{"name":"Biomedical Papers-Olomouc","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10353676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gamma-glutamyltransferase-associated glycoprotein patterns in human seminal plasma of normozoospermic men: a new aspect of biomarker heterogeneity. 正常精子男性精浆中γ -谷氨酰转移酶相关的糖蛋白模式:生物标志物异质性的新方面。

IF 0.9 4区医学

Biomedical Papers-Olomouc Pub Date : 2023-07-17 DOI: 10.5507/bp.2023.031

Tamara Jankovic, Jelena Danilovic Lukovic, Sanja Goc, Ninoslav Mitic, Ljiljana Hajdukovic, Miroslava Jankovic

{"title":"Gamma-glutamyltransferase-associated glycoprotein patterns in human seminal plasma of normozoospermic men: a new aspect of biomarker heterogeneity.","authors":"Tamara Jankovic, Jelena Danilovic Lukovic, Sanja Goc, Ninoslav Mitic, Ljiljana Hajdukovic, Miroslava Jankovic","doi":"10.5507/bp.2023.031","DOIUrl":"https://doi.org/10.5507/bp.2023.031","url":null,"abstract":"Background: Gamma-glutamyltransferase (GGT) is a well-known laboratory biomarker. In spite of high concentration and the possible biomedical importance of estimating GGT in human seminal plasma (hSP), it has not been widely explored in reproductive physiology. This study aimed to complement existing data on its diversity, previously obtained on seminal extracellular vesicles, by analyzing matched soluble fraction of hSP. The GGT-associated patterns of selected glycoproteins were analyzed in order to establish an adjunct referent parameter for differentiation between known high molecular mass forms of GGT. Getting insight into distinct GGT-associated glycoprotein patterns should contribute to define them together as possible multimarkers.Methods: GGT forms in soluble, membrane-free-fraction isolated form hSP of normozoospermic men were analyzed using gel filtration and lectin blotting using WGA (wheat germ agglutinin) and Con A (concanavalin A).Results: Widely distributed GGT (with two to three partially resolved peaks), which may correspond to high molecular mass aggregates, were detected. GGT-associated patterns of selected glycoproteins (at position of big, medium, and small-GGT) all comprised high molecular mass WGA-reactive smears, but differed in the presence of Con A-reactive glycans, as well as mucin-associated antigens CA19-9 and CA125.Conclusions: GGT contributes to several molecular patterns that differ between the soluble and extracellular vesicle fractions of hSP. Their glycobiochemical heterogeneity is due to difference in the presence of distinct sialylated and mannosylated glycans. Moreover, GGT-associated glycoprotein patterns differentiate between high molecular mass forms of GGT in the soluble fraction of hSP. They hold promise as possible targets for increasing biomarker potential of GGT.","PeriodicalId":55363,"journal":{"name":"Biomedical Papers-Olomouc","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10190078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

S100B protein as a biomarker and predictor in traumatic brain injury. S100B蛋白作为外伤性脑损伤的生物标志物和预测因子。

IF 0.9 4区医学

Biomedical Papers-Olomouc Pub Date : 2023-07-10 DOI: 10.5507/bp.2023.025

Stefan Trnka, Premysl Stejskal, Jakub Jablonsky, David Krahulik, Daniel Pohlodek, Lumir Hrabalek

{"title":"S100B protein as a biomarker and predictor in traumatic brain injury.","authors":"Stefan Trnka, Premysl Stejskal, Jakub Jablonsky, David Krahulik, Daniel Pohlodek, Lumir Hrabalek","doi":"10.5507/bp.2023.025","DOIUrl":"https://doi.org/10.5507/bp.2023.025","url":null,"abstract":"Objectives: To determine the prognostic potential of S100B protein in patients with craniocerebral injury, correlation between S100B protein and time, selected internal diseases, body habitus, polytrauma, and season.Methods: We examined the levels of S100B protein in 124 patients with traumatic brain injury (TBI).Results: The S100B protein level 72 h after injury and changes over 72 h afterwards are statistically significant for prediction of a good clinical condition 1 month after injury. The highest sensitivity (81.4%) and specificity (83.3%) for the S100B protein value after 72 h was obtained for a cut-off value of 0.114. For the change after 72 h, that is a decrease in S100B value, the optimal cut-off is 0.730, where the sum of specificity (76.3%) and sensitivity (54.2%) is the highest, or a decrease by 0.526 at the cut-off value, where sensitivity (62.5%) and specificity (62.9%) are more balanced. The S100B values were the highest at baseline; S100B value taken 72 h after trauma negatively correlated with GCS upon discharge or transfer (r=-0.517, P<0.0001). We found no relationship between S100B protein and hypertension, diabetes mellitus, BMI, or season when the trauma occurred. Changes in values and a higher level of S100B protein were demonstrated in polytraumas with a median of 1.070 (0.042; 8.780) μg/L compared to isolated TBI with a median of 0.421 (0.042; 11.230) μg/L.Conclusion: S100B protein level with specimen collection 72 h after trauma can be used as a complementary marker of patient prognosis.","PeriodicalId":55363,"journal":{"name":"Biomedical Papers-Olomouc","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9768229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Life expectancy in glioblastoma patients who had undergone stereotactic biopsy: a retrospective single-center study. 接受立体定向活检的胶质母细胞瘤患者的预期寿命:一项回顾性单中心研究。

IF 0.9 4区医学

Biomedical Papers-Olomouc Pub Date : 2023-07-10 DOI: 10.5507/bp.2023.030

Matej Halaj, Ondrej Kalita, Lucie Tuckova, Lumir Hrabalek, Martin Dolezel, Jana Vrbkova

{"title":"Life expectancy in glioblastoma patients who had undergone stereotactic biopsy: a retrospective single-center study.","authors":"Matej Halaj, Ondrej Kalita, Lucie Tuckova, Lumir Hrabalek, Martin Dolezel, Jana Vrbkova","doi":"10.5507/bp.2023.030","DOIUrl":"https://doi.org/10.5507/bp.2023.030","url":null,"abstract":"Objective: The best results in glioblastoma (GBM) are obtained through aggressive treatment comprising maximally radical but safe resection followed by chemoradiotherapy. However, certain patients will undergo only stereotactic biopsy. This paper aims to evaluate life expectancy in GBM patients who underwent only stereotactic biopsy, including the effect of subsequent oncological treatment.Patients and methods: Patients with confirmed GBM histology who had undergone stereotactic biopsy between June 2006 and December 2016 were retrospectively selected. Each patient had received a CT scan, followed by an MRI scan with a contrast agent. None of the patients were amenable to microsurgical resection.Results: Of the 60 patients, 41 (69%) received no subsequent oncological treatment, while 14 (23%) underwent isolated radiotherapy. Mean survival time of all patients was 2.8 months. Those who received no additional treatment had an average survival time of 2.3 months; patients who received any type of oncological treatment was 3.7 months. Of these, those receiving radiotherapy alone had a mean survival of 3.1 months. Patients who received oncological treatment with the Stupp protocol had a survival time of 6.6 months.Conclusion: Diagnostic and surgical advances related to GBM treatment mean that radical resections can be performed even in eloquent brain areas. However, patients not indicated for resection will experience a major reduction in life expectancy. Patients who underwent stereotactic biopsy and received some form of oncological treatment experienced slightly increased overall survival relative to patients with a natural disease course. Patients with favorable clinical factors reacted better to treatment.","PeriodicalId":55363,"journal":{"name":"Biomedical Papers-Olomouc","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9823340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Population pharmacokinetics of three alternative prophylactic antibiotics during cardiac surgery with extracorporeal circulation. 体外循环心脏手术中三种预防性抗生素的人群药代动力学。

IF 0.9 4区医学

Biomedical Papers-Olomouc Pub Date : 2023-06-01 DOI: 10.5507/bp.2022.033

Petr Santavy, Vendula Kubickova, Martin Sima, Karel Urbanek

{"title":"Population pharmacokinetics of three alternative prophylactic antibiotics during cardiac surgery with extracorporeal circulation.","authors":"Petr Santavy, Vendula Kubickova, Martin Sima, Karel Urbanek","doi":"10.5507/bp.2022.033","DOIUrl":"https://doi.org/10.5507/bp.2022.033","url":null,"abstract":"Aims: The aim of this pharmacokinetic study was to describe and quantify population pharmacokinetics of three antibiotics, cefazolin, ampicillin, and ciprofloxacin, used as antibacterial prophylaxis during cardiovascular surgery with the use of extracorporeal circulation (ECC).Methods: Adult patients undergoing cardiac surgery with ECC were enrolled to this prospective, pharmacokinetic study. An intravenous bolus of 2 g of ampicillin, 2 g of cefazolin or 400 mg of ciprofloxacin was administered 60-30 min before surgery. Blood samples were collected at 15, 30, 45, 60, 120 and 180 min after the administration and at the end of the surgery. Plasma concentrations of the antibiotics were measured using HPLC methods. Serum concentration-time profiles were analyzed using nonlinear mixed-effects modeling approach.Results: A total of 54 patients were enrolled into the study, 20 with ampicillin, 25 cefazolin and 9 ciprofloxacin. For all antibiotics, population pharmacokinetic models have been successfully developed.Conclusion: We identified estimated glomerular filtration rate (eGFR) as the main factor determining the achievement of the pharmacokinetic/pharmacodynamic (PK/PD) target in ampicillin or cefazolin and body weight in ciprofloxacin prophylaxis during cardiac surgery with ECC support.","PeriodicalId":55363,"journal":{"name":"Biomedical Papers-Olomouc","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9620047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Clinical presentation and pulmonary function tests in post-acute COVID-19 patients. COVID-19急性后患者的临床表现和肺功能检测

IF 0.9 4区医学

Biomedical Papers-Olomouc Pub Date : 2023-06-01 DOI: 10.5507/bp.2022.039

Samuel Genzor, Petr Jakubec, Milan Sova, Jan Mizera, Pavol Joppa, Radim Burget, Pavol Pobeha

{"title":"Clinical presentation and pulmonary function tests in post-acute COVID-19 patients.","authors":"Samuel Genzor, Petr Jakubec, Milan Sova, Jan Mizera, Pavol Joppa, Radim Burget, Pavol Pobeha","doi":"10.5507/bp.2022.039","DOIUrl":"https://doi.org/10.5507/bp.2022.039","url":null,"abstract":"Aims: The study analysed post-acute COVID-19 symptoms and the pulmonary function test (PFT) results in patients surviving the native strain of the virus.Methods: The study was prospective; the inclusion criteria were positive PCR test for SARS-CoV-2 and age 18-100. Exclusion criteria were active respiratory infection, known or suspicious pre-existing pulmonary disease, cardiac failure, recent or acute pulmonary embolism, anaemia, and neuromuscular diseases. The recruitment period was 1st March 2020 - 25th December 2020. The initial examination was performed 4-12 weeks after the disease onset. All subjects underwent physical examination, anamnesis, chest x-ray and PFT.Results: The study involved 785 subjects (345 male) mean age 53.8 (SD 14.6). The disease severity groups were: mild (G1), moderate (G2) and severe/critical (G3). Anosmia was present in the acute disease phase in 45.2% of G1 patients, but only in 4.5% of G3 patients. Dyspnoea occurred frequently in more severe groups (40%, 51.8% and 63.7% for G1, G2 and G3 respectively), while cough and fatigue showed no relationship to disease severity. Females were more likely to experience persistent symptoms. PFT results were significantly decreased in more severe groups compared to the mild COVID-19 patients, diffusing capacity was 86.3%, 79% and 68% of predicted values in G1, G2 and G3 respectively.Conclusion: Anosmia during the acute phase was associated with mild disease, persisting dyspnoea was more frequent after more severe COVID-19. Females tended to have persisting symptoms in post-acute phase more frequently. PFT results showed decrease with disease severity.","PeriodicalId":55363,"journal":{"name":"Biomedical Papers-Olomouc","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9628849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Combination of plant phenolics and isoquinolinium alkaloids protects gingival fibroblast and improves post-extraction healing after lower third molar extraction. 植物酚类物质和异喹啉生物碱的结合保护牙龈成纤维细胞，促进下第三磨牙拔牙后的愈合。

IF 0.9 4区医学

Biomedical Papers-Olomouc Pub Date : 2023-06-01 DOI: 10.5507/bp.2021.055

Karin Chytilova, Adela Galandakova, Katerina Valentova, Peter Tvrdy

{"title":"Combination of plant phenolics and isoquinolinium alkaloids protects gingival fibroblast and improves post-extraction healing after lower third molar extraction.","authors":"Karin Chytilova, Adela Galandakova, Katerina Valentova, Peter Tvrdy","doi":"10.5507/bp.2021.055","DOIUrl":"https://doi.org/10.5507/bp.2021.055","url":null,"abstract":"Aims: The effect of polyphenolic fraction of Lonicera caerulea (PFLC) and alkaloid fraction of Macleaya cordata (AFMC) mix on the production of inflammatory mediators in human gingival fibroblasts pretreated with lipopolysaccharide (LPS) was investigated. In addition, protective effects of mucoadhesive paste containing combination of PFLC and AFMC (0.05% and 0.01%, respectively; n=15, Group A) and placebo (n=15, Group B) were evaluated in patients after surgical extraction of lower third molars.Methods: Gingival fibroblasts were pre-treated with LPS (10 µg/mL; 24 h) and PFLC/AFMC (25/0.25; 50/0.25; 100/0.25; 25/0.5; 50/0.5; 100/0.5 µg/mL) in serum-free medium was applied for 4 h. Then the interleukin-6 (IL-6), reactive oxygen species (ROS) generation, level of intracellular glutathione (GSH) and expression of cyclooxygenase-2 (COX-2) were evaluated. The study was a 6-day, single-center, randomized, double-blind and placebo-controlled trial consisting of two parallel treatment arms. A modified Oral health impact profile questionnaire including both general oral condition and extraction related questions, was used to evaluate the oral condition and other changes before (day 0) and on the days 1, 3 and 6 after surgical extraction.Results and conclusion: The combination of PFLC with AFMC caused a reduction of ROS generation, reduced IL-6 production and suppressed the expression of COX-2. In group A the paste treatment contributed to improvement of oral health-related quality of life. Topical application of PFLC and AFMC into the extraction wound improved post-extraction site wound healing probably by antioxidant and anti-inflammatory mechanisms.","PeriodicalId":55363,"journal":{"name":"Biomedical Papers-Olomouc","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9619577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0