Biotechnology & Genetic Engineering Reviews最新文献_第7页

CXCR2 (rs3890158 and rs2230054) and CXCL4 (rs352008 and rs1801572) gene polymorphisms in patients with thoracic aortic aneurysm. 胸主动脉瘤患者的 CXCR2（rs3890158 和 rs2230054）和 CXCL4（rs352008 和 rs1801572）基因多态性。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-05-08 DOI: 10.1080/02648725.2023.2210011

Hua Zhao, Yanrong Xu, Jun Cui

{"title":"CXCR2 (rs3890158 and rs2230054) and CXCL4 (rs352008 and rs1801572) gene polymorphisms in patients with thoracic aortic aneurysm.","authors":"Hua Zhao, Yanrong Xu, Jun Cui","doi":"10.1080/02648725.2023.2210011","DOIUrl":"10.1080/02648725.2023.2210011","url":null,"abstract":"We aimed to explore the correlations of C-X-C motif chemokine receptor 2 (CXCR2) and chemokine (C-X-C motif) ligand 4 (CXCL4) gene polymorphisms with thoracic aortic aneurysm. A total of 50 patients with thoracic aortic aneurysm (disease group) and 50 healthy people in the physical examination center (control group) in our hospital were selected as the subjects. The CXCR2 and CXCL4 gene polymorphisms were detected by means of blood drawing, DNA extraction, PCR and sequencing. Moreover, the levels of serum CXCR2 and CXCL4 were measured using ELISA, and the levels of C-reactive protein (CRP) and low-density lipoprotein (LDL) were determined. The study found significant differences in the distribution of genotypes and alleles of CXCR2 and CXCL4 gene polymorphisms between the disease group and control group. The frequencies of certain genotypes (AA of rs3890158, CC of rs2230054, AT of rs352008, and CT of rs1801572) were higher in the disease group, as were the frequencies of certain alleles (C of rs2230054 and rs1801572). The distribution of recessive models of rs2230054 was also different, with a lower frequency of CC+CT in the disease group. The haplotype distributions of both gene polymorphisms differed between the groups. CXCR2 rs3890158 and CXCL4 rs352008 were correlated with lower serum levels of their respective proteins, while CXCL4 rs1801572 was associated with CRP levels and CXCR2 rs2230054 with LDL levels in patients (P < 0.05). The gene polymorphisms of CXCR2 and CXCL4 probably have apparent correlations with the susceptibility to thoracic aortic aneurysm.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4300-4312"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9437021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioinformatics analysis illustrates the functions of miR-377-5p in cervical cancer. 生物信息学分析说明了 miR-377-5p 在宫颈癌中的功能。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-05-05 DOI: 10.1080/02648725.2023.2208453

Dongjie Wang, Yifeng Zhang, Dongyan Ren, Chunmei Meng, Liufeng Yang

{"title":"Bioinformatics analysis illustrates the functions of miR-377-5p in cervical cancer.","authors":"Dongjie Wang, Yifeng Zhang, Dongyan Ren, Chunmei Meng, Liufeng Yang","doi":"10.1080/02648725.2023.2208453","DOIUrl":"10.1080/02648725.2023.2208453","url":null,"abstract":"Cervical cancer (CC) is a frequent disease in women whose development is related with miRNA disorder. MiR-377-5p plays a negative role in the development of some tumors, while few studies have revealed its role in CC. In this study, the functions of miR-377-5p in CC were investigated by bioinformatics. Briefly, the expression and survival curve of miR-377-5p in CC was analyzed with the Cancer Genome Atlas (TCGA) database, and the abundance of miR-377-5p in clinical samples and CC cell lines were measured by qRT-PCR. Moreover, the MicroRNA Data Integration Portal (miRDIP) database was used to predict targets of miR-377-5p, and the Database for Annotation Visualization and Integrated Discovery (David) was used for enrichment analysis of the functions of the miR-377-5p. The Search Tool for the Retrieval of Interacting Genes (STRING) database was used to screen the hub targets of miR-377-5p. Moreover, the Gene Expression Profiling Interactive Analysis (GEPIA) database was used to analyze the abundance of the genes in CC. Results showed that decreased miR-377-5p was found in the CC tissues and cell lines, and low miR-377-5p was connected with poor prognosis of patients. Besides, the targets of miR-377-5p were enriched in the PI3K/AKT, MAPK and RAS signaling pathways. Moreover, CDC42, FLT1, TPM3 and CAV1 were screened as hub nodes in the targets of miR-377-5p, and increased CDC42, FLT1, TPM3 and CAV1 also indicated the poor survival rates of the patients in the long term. In conclusion, this study suggests that miR-377-5p downregulation is a biomarker event for CC progression.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4238-4249"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9763569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antidiabetic effect of flavonoid from Rumex vesicarius on alloxan induced diabetes in Male Albino Wistar rats and its validation through in silico molecular docking and dynamic simulation studies. 鲁梅克斯黄酮对雄性白化Wistar大鼠阿脲诱导糖尿病的抗糖尿病作用及其通过硅分子对接和动态模拟研究进行的验证

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-05-16 DOI: 10.1080/02648725.2023.2213042

Abeer A Al-Masri, Fuad Ameen, Rakesh Davella, Estari Mamidala

{"title":"Antidiabetic effect of flavonoid from Rumex vesicarius on alloxan induced diabetes in Male Albino Wistar rats and its validation through in silico molecular docking and dynamic simulation studies.","authors":"Abeer A Al-Masri, Fuad Ameen, Rakesh Davella, Estari Mamidala","doi":"10.1080/02648725.2023.2213042","DOIUrl":"10.1080/02648725.2023.2213042","url":null,"abstract":"The leaves of Rumex vescarius L. are used locally to treat diabetes, a chronic illness. A flavonoid called Luteolin from R. vesicarius was chosen to explore for the antidiabetic potential through the in vivo antidiabetic test against male albino Wistar rats that had been induced with diabetes due to alloxan. Additionally, docking screening was carried out with the aid of autodock software to identify probable moiety that might be in charge of its anti-diabetic effect. Given at a dose of 100 mg/kg body weight, luteolin from R. vesicarius leaves had a significant (p < 0.05) hypoglycaemic impact after just one week. The blood glucose level significantly decreased during the third week (p < 0.05). All provided doses of luteolin from R. vesicarius leaves resulted in a reduction, however on all study days, the highest concentration (400 mg/kg body weight) produced the biggest reduction. The results of luteolin's molecular docking and dynamic modelling studies with a variety of targets revealed significant binding interactions at the active site binding pocket, with the target α-glucosidase having the highest binding affinity (-9.35 kcal/mol). In conclusion, the plant and the flavonoid luteolin it contains have potent anti-diabetic properties, possibly through an interaction with the enzyme α-glucosidase.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4479-4494"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9823920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Data-based analysis of psychological and emotional changes in breast cancer patients at different ages before and after surgery. 基于数据分析不同年龄乳腺癌患者手术前后的心理和情绪变化。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-05-18 DOI: 10.1080/02648725.2023.2207936

Songling Liu, Yue Wan, Xi Yu, Yalin Zhang, Lin Shen, Tianhui Su, Xiao Zou, Zheng Yan, Kaihua Zhao

{"title":"Data-based analysis of psychological and emotional changes in breast cancer patients at different ages before and after surgery.","authors":"Songling Liu, Yue Wan, Xi Yu, Yalin Zhang, Lin Shen, Tianhui Su, Xiao Zou, Zheng Yan, Kaihua Zhao","doi":"10.1080/02648725.2023.2207936","DOIUrl":"10.1080/02648725.2023.2207936","url":null,"abstract":"To analyze psychological and emotional changes in breast cancer patients at different ages before and after surgery based on data. The clinical data of 363 patients undergoing radical mastectomy for breast cancer in our hospital from December 2019 to December 2021 were selected for retrospective analysis. The patients' psychological and emotional changes before and after surgery were evaluated with the mental health symptom self-rating scale, and patients' quality of life was assessed by World Health Organization Quality of Life (WHOQOL)-BREF. On a whole, no significant differences in the patients' scores on somatization, interpersonal sensitivity, dreadness and others before and after surgery were observed (P > 0.05), while their scores on obsessive-compulsive symptom, depression, anxiety, hostility, paranoid ideation and psychopathy as well as the total scores were significantly different (P < 0.05); before and after surgery, patients' psychological scores were not significantly different among the five groups (P > 0.05), but various WHOQOL-BREF scores were significantly different (P < 0.05). Surgical treatment has little impact on the psychological mood of breast cancer patients, obvious difference in quality of life is presented among patients at different ages before and after surgery, and therefore targeted clinical intervention should be given.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4193-4204"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9851345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering sperm functions using biological networks. 利用生物网络解密精子功能。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-02-01 DOI: 10.1080/02648725.2023.2168912

Naseer A Kutchy, Olanrewaju B Morenikeji, Aylin Memili, Muhammet R Ugur

{"title":"Deciphering sperm functions using biological networks.","authors":"Naseer A Kutchy, Olanrewaju B Morenikeji, Aylin Memili, Muhammet R Ugur","doi":"10.1080/02648725.2023.2168912","DOIUrl":"10.1080/02648725.2023.2168912","url":null,"abstract":"The global human population is exponentially increasing, which requires the production of quality food through efficient reproduction as well as sustainable production of livestock. Lack of knowledge and technology for assessing semen quality and predicting bull fertility is hindering advances in animal science and food animal production and causing millions of dollars of economic losses annually. The intent of this systemic review is to summarize methods from computational biology for analysis of gene, metabolite, and protein networks to identify potential markers that can be applied to improve livestock reproduction, with a focus on bull fertility. We provide examples of available gene, metabolic, and protein networks and computational biology methods to show how the interactions between genes, proteins, and metabolites together drive the complex process of spermatogenesis and regulate fertility in animals. We demonstrate the use of the National Center for Biotechnology Information (NCBI) and Ensembl for finding gene sequences, and then use them to create and understand gene, protein and metabolite networks for sperm associated factors to elucidate global cellular processes in sperm. This study highlights the value of mapping complex biological pathways among livestock and potential for conducting studies on promoting livestock improvement for global food security.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"3743-3767"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10592573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of lncRNA DLX6-AS1 in patients with hepatic carcinoma and its prognostic value. 肝癌患者中 lncRNA DLX6-AS1 的表达及其预后价值。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-04-20 DOI: 10.1080/02648725.2023.2204706

Yonghua Lin, Baoyan Zhang, Linfeng Su, Jianfeng Wei, Zhichao Chen, Min Wu

{"title":"Expression of lncRNA DLX6-AS1 in patients with hepatic carcinoma and its prognostic value.","authors":"Yonghua Lin, Baoyan Zhang, Linfeng Su, Jianfeng Wei, Zhichao Chen, Min Wu","doi":"10.1080/02648725.2023.2204706","DOIUrl":"10.1080/02648725.2023.2204706","url":null,"abstract":"Hepatic carcinoma (HCC) is one of the most common malignant tumors worldwide, and the prognosis of HCC patients is often poor. Long-chain non-coding RNA (lncRNA) distal-less homeobox 6 antisense 1 (DLX6-AS1) has been shown to be involved in the pathogenesis of various cancers. This study aims to investigate the expression of DLX6-AS1 in HCC patients and its prognostic value. The serum DLX6-AS1 was quantified using a reverse transcription-polymerase chain reaction (RT-PCR) assay in both HCC patients and healthy individuals, and the correlation of DLX6-AS1 with clinicopathological features of HCC patients, as well as the diagnostic and prognostic value of DLX6-AS1 for HCC patients, were analyzed. The results showed that the expression of serum DLX6-AS1 in HCC patients was significantly higher than that of healthy individuals (P < 0.05), and DLX6-AS1 was related to tumor differentiation, pathological staging, and lymph node metastasis (all P < 0.05). Patients with high DLX6-AS1 expression showed significantly higher mortality than those with low DLX6-AS1 expression, and the DLX6-AS1 expression in dead patients was significantly higher than that in living patients. Furthermore, the AUC of DLX6-AS1 for poor prognosis of HCC patients was larger than 0.8. The univariate analysis revealed that the poor prognosis of HCC patients was related to pathological staging, lymph node metastasis, differentiation, and DLX6-AS1 expression (all P < 0.05), and the Cox multivariate analysis revealed that pathological staging, lymph node metastasis, differentiation, and DLX6-AS1 expression were independent risk factors for poor prognosis of HCC patients (all P < 0.05). These findings suggest that DLX6-AS1 may be a promising target for diagnosis, treatment, and prognosis prediction of HCC patients.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"3976-3987"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9384483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of ZnO/Gypsum/Gelatine nanocomposites films and their antibacterial mechanism against Staphylococcus aureus. 氧化锌/石膏/明胶纳米复合材料薄膜的制备及其对金黄色葡萄球菌的抗菌机制

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-05-27 DOI: 10.1080/02648725.2023.2216419

Susanta Kumar Behera, Mashael Huwaikem, Bhumika Jena, Maulin P Shah, Sankha Chakrabortty, Suraj K Tripathy, Amrita Mishra

{"title":"Fabrication of ZnO/Gypsum/Gelatine nanocomposites films and their antibacterial mechanism against Staphylococcus aureus.","authors":"Susanta Kumar Behera, Mashael Huwaikem, Bhumika Jena, Maulin P Shah, Sankha Chakrabortty, Suraj K Tripathy, Amrita Mishra","doi":"10.1080/02648725.2023.2216419","DOIUrl":"10.1080/02648725.2023.2216419","url":null,"abstract":"Staphylococcus aureus (S. aureus) has long been acknowledged as being one of the most harmful bacteria for human civilization. It is the main contributor to skin and soft tissue infections. The gram positive pathogen also contributes to bloodstream infections, pneumonia, or bone and joint infections. Hence, developing an efficient and targeted treatment for these illnesses is greatly desired. Recently, studies on nanocomposites (NCs) have significantly increased due to their potent antibacterial and antibiofilm properties. These NCs provide an intriguing way to control the growth of bacteria without causing the development of resistance strains that come from improper or excessive use of the conventional antibiotics. In this context, we have demonstrated the synthesis of a NC system by precipitation of ZnO nanoparticles (NPs) on Gypsum followed by encapsulation with Gelatine, in the present study. Fourier transform infrared (FTIR) spectroscopy was used to validate the presence of ZnO NPs and Gypsum. The film was characterized by X-ray diffraction (XRD) spectroscopy and scanning electron microscopy (SEM). The system exhibited promising antibiofilm action and was effective in combating S. aureus and MRSA in concentrations between 10 and 50 ug/ml. The bactericidal mechanism by release of reactive oxygen species (ROS) was anticipated to be induced by the NC system. Studies on cell survival and in-vitro infection support the film's notable biocompatibility and its potential for treating Staphylococcus infections in the future.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4713-4736"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9898489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Shouwu Yizhi prescription in decubation of patients with ischemic stroke. 首乌翼方在缺血性脑卒中患者拔管中的应用

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-04-17 DOI: 10.1080/02648725.2023.2203005

Chao Chen, Guangqing Cheng, Ruiqi Jia, Aishuai Wang, Anrong Wang, Xiaoni Yang

{"title":"Application of Shouwu Yizhi prescription in decubation of patients with ischemic stroke.","authors":"Chao Chen, Guangqing Cheng, Ruiqi Jia, Aishuai Wang, Anrong Wang, Xiaoni Yang","doi":"10.1080/02648725.2023.2203005","DOIUrl":"10.1080/02648725.2023.2203005","url":null,"abstract":"We investigate the application of Shouwu Yizhi prescription (SYP) in decubation of patients with ischemic stroke (IS). The clinical data of 106 patients recovering from IS who came to our hospital from December 2019 to December 2020 were selected for retrospective analysis, and they were separated into experimental group (n = 53, basic treatment + SYP) and control group (n = 53, basic treatment) based on the principle of random grouping. The clinical indexes such as lipid indexes and neurological disability score (NDS) after treatment were compared between both groups to comprehensively evaluate the clinical effects of different treatment regimens. Except for high-density lipoprotein cholesterol value, the lipid indexes in the experimental group after treatment were remarkably lower than those in the control group (P < 0.001). After treatment, the levels of hypersensitive C-reactive protein, homocysteine and lipoprotein-associated phospholipase A2 were remarkably lower in the experimental group than control group (P < 0.05). After treatment, the experimental group had remarkably higher mean scores of Montreal Cognitive Assessment, Fugl-Meyer Assessment in upper and lower limbs and lower NDS than control group (P < 0.001). SYP is an efficient treatment plan in decubation of IS, which can effectively improve the blood lipid indexes and neurological function of patients, and further studies will help establish a better solution for such patients.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"3035-3046"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9310622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endophytic fungi associated with Brucea mollis Wall. ex Kurz.: a hidden source of antimicrobial and antioxidant metabolites. 与 Brucea mollis Wall.

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-05-26 DOI: 10.1080/02648725.2023.2216967

Deepanwita Deka, Dhruva Kumar Jha

{"title":"Endophytic fungi associated with Brucea mollis Wall. ex Kurz.: a hidden source of antimicrobial and antioxidant metabolites.","authors":"Deepanwita Deka, Dhruva Kumar Jha","doi":"10.1080/02648725.2023.2216967","DOIUrl":"10.1080/02648725.2023.2216967","url":null,"abstract":"Geosmithia pallida (KU693285) was isolated from Brucea mollis an endangered medicinal plant of North-East India. The secondary metabolites, produced by the endophytic fungi, extracted by ethyl acetate were screened for antimicrobial activity. G. pallida extract displayed the highest antimicrobial activity against Candida albicans with a minimum inhibitory concentration of 80.5 ± 1.25 µg/mL. G. pallida also showed the highest antioxidant activity which differed insignificantly from Penicillium sp. (P > 0.05). The G. pallida extract also exhibited the highest cellulase activity and also amylase and protease activities. The cytotoxicity assay of the ethyl acetate extract of this endophyte showed negligible effect (1.93 ± 0.42%) on chromosomal aberration as compared to the control (cyclophosphamide monohydrate) (7.20 ± 1.51%). The internal transcribed spacer rDNA sequence of G. pallida was submitted to the NCBI (Accession number KU693285) from India for the first time. The FT-IR spectrophotometry of the bioactive metabolite of G. pallida showed the presence of different functional groups such as alcohol, carboxylic acids, amines, aromatics, alkyl halides, aliphatic amines and alkynes. The GC-MS analysis revealed the presence of acetic acid, 2-phenylethyl ester; tetracosane; cyclooctasiloxane hexadecamethyl; cyclononasiloxane octadecamethyl; octadecanoic acid; phthalic acid, di(2-propylpentyl) ester and nonadecane, 2,6,10,14,18-pentamethyl as the major compounds in the metabolite. The findings of the present work indicated G. pallida as a potential source of important biomolecules without mammalian cytotoxic effects, which can be utilized for pharmaceutical purposes.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4825-4848"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9527242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of genetic test results in 378 patients suspected of thalassaemia. 对 378 名地中海贫血症疑似患者的基因检测结果进行分析。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-05-24 DOI: 10.1080/02648725.2023.2210015

Jing Jin, Weiying Feng, Zehao Fang, Jiaping Fu, Hongqiang Luo, Pan Hong, Li Hong, Lin Zhang

{"title":"Analysis of genetic test results in 378 patients suspected of thalassaemia.","authors":"Jing Jin, Weiying Feng, Zehao Fang, Jiaping Fu, Hongqiang Luo, Pan Hong, Li Hong, Lin Zhang","doi":"10.1080/02648725.2023.2210015","DOIUrl":"10.1080/02648725.2023.2210015","url":null,"abstract":"Objective: To analyze the genetic test results of 378 patients suspected of thalassemia.Methods: 378 suspected thalassemia patients in Shaoxing People's Hospital from 2014 to 2020 were selected and venous blood was tested using Gap-PCR and PCR-reversed dot blottin. The distribution of genotypes and other information of gene-positive patients was observed.Results: Thalassemia genes were detected in 222 cases, with an overall detection rate of 58.7%, of which 41.4% were α deletion type, 1.35% were α dot, 52.7% were α thalassemia, and 4.5% were αβ complex type. Among the 86 people with provincial household registration, the α-thalassemia gene accounted for 65.1% and the β-thalassemia gene accounted for 25.6%. Follow-up found that Shaoxing nationality accounted for 53.1% of positive patients, of which β-thalassemia gene accounted for 72.9% and α-thalassemia gene accounted for 25.4%; other cities in the province accounted for 8.1% of the total. Other provinces and cities accounted for 38.7%, most of which were from Guangxi and Guizhou. Among all positive patients, the most common α-thalassemia genotypes were --sea / αα, --α / αα,--α 3.7 4.2 / αα , --α3.7 / --sea. The most common mutations in β-thalassemia were IVS-II-654, CD41-42, CD17 and CD14-15.Conclusion: The thalassemia gene carrier status was sporadically distributed outside the traditional thalassemia high prevalence areas. The local population in Shaoxing has a high detection rate of thalassemia genes, and the genetic composition is different from the traditional high prevalence area of thalassemia in the south.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4313-4327"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9570450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0