Bratislava Medical Journal-Bratislavske Lekarske Listy最新文献

{"title":"Tannic Acid and Pamidronate Synergistically Modulate Osteoblast and Osteoclast Activity in a Human Co-Culture Model","authors":"Nurul Husna Azizul, Hermizi Hapidin, Hasmah Abdullah, Maryam Azlan, Azlina Ahmad, Soelaiman Ima-Nirwana","doi":"10.1007/s44411-025-00401-4","DOIUrl":"https://doi.org/10.1007/s44411-025-00401-4","url":null,"abstract":"and Background. To evaluates the effects of tannic acid (TA) combined with pamidronate on human osteoblast (hFOB 1.19) and osteoclast co-culture model. The half-maximal effective concentration (EC50) for TA, pamidronate, and their combinations (ratios: 25:75, 50:50, 75:25) were determined via MTT assay. Drug interactions were assessed using the combination index, while cell proliferation was analyzed with the trypan blue assay. Morphological changes were examined using phase-contrast inverted and scanning electron microscopes (SEM). Alkaline phosphatase (ALP) activity and mineral deposition were evaluated using histochemical assays (ALP, Alizarin Red S, and von Kossa). Mineral quantification was performed with SEM–EDX. TA and the 50:50 TA: PAM combination demonstrated potent effects, with EC50 values of 0.004 µg/mL and 0.007 µg/mL, respectively, and showed a synergistic interaction. Cells treated with the combination drugs exhibited significantly increased proliferation on days 7 and 10 compared to single treatments or controls. Morphological analysis showed that treatments reduced OCs size while promoting OBs activity. All treatments enhanced ALP activity and calcium deposition, with single treatment inducing calcium deposition from day 3, and the combination treatment showing marked increases from day 7. Notably, phosphate deposition was significantly higher at all time points with the combination treatment. However, prolonged exposure to pamidronate reduced osteoblast proliferation and mineralization by day 14 due to adverse effects. A 50:50 combination of TA and pamidronate effectively reduces OCs size while enhancing OBs mineralization and proliferation. Despite the potential adverse effects of prolonged pamidronate exposure, the combination shows promise for promoting bone formation.","PeriodicalId":55328,"journal":{"name":"Bratislava Medical Journal-Bratislavske Lekarske Listy","volume":"127 2","pages":"612-636"},"PeriodicalIF":0.0,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147330650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mulberroside A Alleviates Myocardial Infarction by Inhibiting Oxidative Stress and Apoptosis Via the PI3K/Akt Signaling Pathway","authors":"Taimin Liu, Qian Zhang, Fan Zhao, Jun Yin, Huidi Liu, Li Wang, Bo Liu","doi":"10.1007/s44411-025-00197-3","DOIUrl":"https://doi.org/10.1007/s44411-025-00197-3","url":null,"abstract":"Myocardial infarction (MI), a coronary-related condition that poses a significant threat to life, featured by the occurrence of sudden cardiac death. Cortex Mori radices extract can attenuate myocardial injury in diabetic rats. The study aimed to explore the role of mulberroside A (MBA), a component from Cortex Mori, in isoproterenol (ISO)-induced mouse model of MI. Hematoxylin–eosin (H&E) staining of mouse cardiac tissues was performed to measure pathological changes in context of ISO and/or MBA treatment. Serum levels of lactate dehydrogenase (LDH) and brain natriuretic peptide (BNP) were assessed to examine myocardium damage. Transthoracic echocardiography was used to analyze ejection fraction (EF) and fractional shortening (FS). Expression levels of fibrotic markers were measured by RT-qPCR and western blotting. Serum levels of cardiac function indicator (creatine kinase) and oxidative stress markers were measured using corresponding kit. Protein levels of apoptotic markers and factors associated with the PI3K/Akt signaling were measured by western blotting. MBA alleviated ISO-induced cardiac damage and lowered high expression levels of cardiac fibrosis indicators in the mouse model of MI. MBA significantly improved ISO-induced cardiac dysfunction in mice. Additionally, the inactivation of the p-PI3K and p-Akt signaling induced by ISO was rescued by MBA administration. The inhibition of the PI3K/Akt signaling using LY294002 counteracted the anti-apoptotic and anti-oxidative effects mediated by MBA in MI mouse model. MBA ameliorates ISO-induced myocardial injury by suppressing cardiomyocyte apoptosis and improving oxidative injury via activation of the PI3K/Akt signaling.","PeriodicalId":55328,"journal":{"name":"Bratislava Medical Journal-Bratislavske Lekarske Listy","volume":"126 9","pages":"2148-2156"},"PeriodicalIF":0.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147330321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor Cell-Derived Exosomal METTL3 Induces Choriocarcinoma Malignant Progression by Targeting Fbxw8","authors":"Wenzhi Wang, Haijun Yu, Zhuo Deng, Jin Wen","doi":"10.1007/s44411-025-00111-x","DOIUrl":"https://doi.org/10.1007/s44411-025-00111-x","url":null,"abstract":"Choriocarcinoma (CC) is a highly malignant tumor that occurs in women. Methyltransferase-like 3 (METTL3) is a key protein of m6A methyltransferase complex and its role in CC has been studied, but the study of exosomal METTL3 in CC has not been reported. In this study, quantitative real-time polymerase chain reaction (qRT-PCR) and western blot were used to assess the expressions of related genes. Cell counting kit-8 (CCK-8) assay, wound healing assay, transwell assay, and relevant kit assays were employed to detect the behaviors of tumor cells after different treatments. Moreover, exosome-related researches were performed using kits and transmission electron microscope. Online software was applied to predict the relationship between METTL3 and F-box and WD repeat domain containing 8 (Fbxw8), verified by methylated RNA immunoprecipitation (MeRIP)-qPCR. Furthermore, xenograft mouse models were constructed for validation experiments in vivo. METTL3 was overexpressed in CC cell lines, and was a promoter of CC progression and glycolysis. In addition, METTL3 was highly expressed in tumor cell-derived exosomes and decreased with the addition of GW4869, an exosome inhibitor. Similarly, the knockdown of tumor cell-derived METTL3 also inhibited CC progression and glycolysis. Mechanically, tumor cell-derived exosomal METTL3 motivated the malignant progression of CC by mediating m6A methylation and expression of Fbxw8. In vivo, tumor cell-derived exosomal METTL3 also expedited tumor growth through Fbxw8. Tumor cell-derived exosomal METTL3 promotes glycolysis in CC via regulating m6A methylation modification on Fbxw8 mRNA to further amplify tumor malignant progression, and this will provide a new target for the treatment of CC in the future.","PeriodicalId":55328,"journal":{"name":"Bratislava Medical Journal-Bratislavske Lekarske Listy","volume":"126 7","pages":"1307-1318"},"PeriodicalIF":0.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147330920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compression syndrome of distal peripheral aneurysms of the upper limb.","authors":"Simon Smoter, Roman Slysko","doi":"10.4149/BLL_2024_64","DOIUrl":"10.4149/BLL_2024_64","url":null,"abstract":"<p><p>True aneurysm of the radial artery is very rare. Aneurysmal expansion of arteries due to degenerative changes, possibly infections, primarily affects the abdominal and thoracic aorta, intra and extracranial sections of cerebral arteries, popliteal artery, and visceral arteries. Published literature does not address the aneurysm on the distal sections of the arteries of upper or lower limb. Unlike the classic symptoms of aneurysmally altered arteries such as rupture, thrombosis and embolization, we encounter more often vascular compression syndrome in distal peripheral aneurysms. We demonstrate the case management of a patient with over 20 years increasing wrist resistance. A fusiform aneurysm of the distal section of the radial artery was identified by sonography. Under general anesthesia, we performed aneurysm resection and artery reconstruction using an interpositum from the ipsilateral cephalic vein. The histological examination of the resected tissue confirmed the presence of all three layers of the vascular wall, confirming the true aneurysm of the radial artery. No complications developed in the patient in the postoperative period and all problems related to the aneurysm subsided (Fig. 4, Ref. 23). Keywords: aneurysm, arteria radialis, surgical reconstruction.</p>","PeriodicalId":55328,"journal":{"name":"Bratislava Medical Journal-Bratislavske Lekarske Listy","volume":"125 7","pages":"419-422"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141472889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the relationship between anxiety-depression, systemic immune-inflammation index and clinical progression in COVID-19.","authors":"Begum Aygen Gumus, Ali Emre Sevik, Sevil Alkan","doi":"10.4149/BLL_2024_92","DOIUrl":"https://doi.org/10.4149/BLL_2024_92","url":null,"abstract":"<p><strong>Objective: </strong>Given the multisystem nature of COVID-19 and its potential neuro-psychiatric effects along with the recognized role of systemic inflammation in the prognosis of both COVID-19 and psychiatric disorders, it is imperative to assess psychiatric symptoms in COVID-19 patients. This study sought to investigate the value of systemic immune-inflammation index (SII) scores, levels of anxiety and depressive symptoms assessed within the initial 24 hours following COVID-19 diagnosis as potential predictors of the clinical trajectory of COVID-19.</p><p><strong>Methods: </strong>This study involved 64 patients admitted to our COVID-19 ward with mild-to-moderate COVID-19 pneumonia, all of whom underwent a psychiatric evaluation within 24 hours of admission. Upon admission, levels of c-reactive protein and inflammatory markers including leukocyte, neutrophil, thrombocyte, and lymphocyte counts were measured to calculate individual SII scores. Psychiatric evaluations were conducted using the State-Trait Anxiety Inventory (STAI), Hamilton Depression Rating Scale (HDRS), and Standardized Mini-Mental Test (SMMT).</p><p><strong>Results: </strong>The patients with clinical deterioration of COVID-19 exhibited higher STAI-Trait and STAI-State subscale scores measured upon admission compared to those without clinical deterioration. HDRS scores showed no significant correlation with clinical deterioration. STAI-State subscale scores correlated with SII scores and the duration of hospital stay. High baseline STAI scores and SII scores predicted COVID-19 clinical deterioration.</p><p><strong>Conclusion: </strong>Our study demonstrated that the initial SII and STAI scores assessed within the initial 24 hours of hospitalization for COVID-19 significantly predicted the clinical progression of the disease during the hospital stay (Tab. 5, Ref. 37). Text in PDF www.elis.sk Keywords: COVID-19, systemic inflammatory response index, disease severity, inflammation.</p>","PeriodicalId":55328,"journal":{"name":"Bratislava Medical Journal-Bratislavske Lekarske Listy","volume":"125 10","pages":"589-596"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142332782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of interferon gamma release assay to measure t-cell response in COVID-19 patients from intensive care units and inpatient departments.","authors":"Zainab Khairullah Sedeeq, Ruqiyya Samadzade, Hatice Turk Dagi, Jale Bengi Celik, Onur Ural, Duygu Findik","doi":"10.4149/BLL_2024_83","DOIUrl":"10.4149/BLL_2024_83","url":null,"abstract":"<p><strong>Background: </strong>Interferon gamma release assay (IGRA) is an in vitro blood test to measure interferon gamma (IFN-γ) released from antigen-specific T cells after stimulation with pathogen-specific peptides. In this study, it was aimed to investigate the T-cell response using IGRA and to compare various laboratory values in Coronavirus Disease (COVID-19) patients hospitalized either in hospital inpatient departments or in intensive care units.</p><p><strong>Methods: </strong>A total of 100 patients (50+50) who were identified as positive for COVID-19 through the molecular method in Selcuk University Faculty of Medicine Infectious Diseases Service and Reanimation Intensive Care Unit were included in the study. IFN-γ levels in blood samples collected from patients were determined using the QuantiFERON Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) (QIAGEN, Germany) kit. The patients' gender, age, c-reactive protein (CRP), aspartate aminotransferase (AST), alanine transaminase (ALT), interleukin (IL)-6, lymphocyte count, procalcitonin, and D-dimer results were obtained from the hospital automation system.</p><p><strong>Results: </strong>Thirty-eight of the IGRA test results were negative, 44 were positive and 18 were inconclusive. The age of patients with negative IGRA test results was significantly higher (p<0.001) compared to patients with positive results. There were no significant differences between patients' IGRA test results and gender, prognosis, IL-6, lymphocyte counts, CRP, AST, and ALT values.Age, death rates, D-dimer, CRP, procalcitonin, AST and ALT values of patients hospitalized in the intensive care unit were significantly higher (p<0.001) compared to the those hospitalized in the inpatient department, while conversely, the lymphocyte values were lower (p<0.001).</p><p><strong>Conclusion: </strong>The relatively higher IGRA negative results in the elderly, negative and intermediate results in intensive-care patients, and low lymphocyte levels in intensive-care patients indicate that the cellular immune response is diminished and/or absent. The death rates, D-dimer, CRP, procalcitonin, AST and ALT values of the patients hospitalized in the intensive care unit were higher compared to those from the in-patient department, indicating the severity of inflammation and signaling the development of organ failure. In the light of these findings, we suggest that IGRA tests may serve as a guide in immunomodulatory therapy (Tab. 2, Fig. 2, Ref. 27). Text in PDF www.elis.sk Keywords: COVID-19, interferon gamma release assay test, T cell response.</p>","PeriodicalId":55328,"journal":{"name":"Bratislava Medical Journal-Bratislavske Lekarske Listy","volume":" ","pages":"533-538"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141581595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors for self-reported alcohol consumption during pregnancy.","authors":"Jozef Zahumensky, Maros Boda, Veronika Serator, Petra Psenkova, Michaela Ostatnikova, Martina Gajdosova, Patricia Peskovicova, Simona Dandarova, Martina Sirotna, Peter Papcun","doi":"10.4149/BLL_2024_101","DOIUrl":"https://doi.org/10.4149/BLL_2024_101","url":null,"abstract":"<p><strong>Background: </strong>Fetal alcohol syndrome (FAS) is the most common preventable cause of impaired fetal development. The amount of alcohol consumed by expectant mothers varies by country. Studies on the prevalence of alcohol use among pregnant women in Slovakia and the risk factors linked to alcohol consumption during pregnancy are scarce. Similarly lacking are the data regarding pregnant women's awareness of the negative consequences of alcohol use during pregnancy.</p><p><strong>Methods: </strong>The study comprised women who gave birth at the 2nd Department of Gynecology and Obstetrics between July 1, 2023, and December 31, 2023. All respondents received an anonymous retrospective questionnaire following childbirth. The questionnaire investigated alcohol consumption throughout the year preceding conception as well as during each of the trimesters. The questionnaire also included questions on the woman's plans to become pregnant, her demographics, and her awareness of the hazards associated with alcohol consumption during pregnancy.</p><p><strong>Results: </strong>A filled-out questionnaire was obtained from 402 women. Of these, 51 women (12.8%) reported being completely abstinent before becoming pregnant. Complete abstinence before pregnancy was the greatest strongest protection against drinking during pregnancy (RR: 0.03). Among the 351 women who drank before pregnancy, 108 women (30.8%) continued to drink while pregnant, which constitutes 26.9% of all pregnant women. Risk factors of alcohol use during pregnancy included unplanned pregnancy (RR: 1.32), inadequate knowledge of the negative consequences of alcohol intake during pregnancy (71.9% vs 75.4%), and heavy drinking prior to pregnancy (RR: 2.55).</p><p><strong>Conclusions: </strong>In accordance with certain European data, the best means of protection against alcohol consumption during pregnancy include complete abstinence prior to conception, pregnancy planning, and high-quality education regarding the negative effects of alcohol during pregnancy (Tab. 4, Ref. 19). Text in PDF www.elis.sk Keywords: fetal alcohol syndrome spectrum, alcohol drinking in pregnancy, pregnancy planning, prenatal alcohol exposure, pregnancy.</p>","PeriodicalId":55328,"journal":{"name":"Bratislava Medical Journal-Bratislavske Lekarske Listy","volume":"125 11","pages":"661-665"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142565294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of sample collection without virus transport medium in suspected enteroviral infections for molecular diagnosis.","authors":"Maria Borsanyiova, Shubhada Bopegamage, Sandor George Vari","doi":"10.4149/BLL_2024_33","DOIUrl":"10.4149/BLL_2024_33","url":null,"abstract":"<p><p>Clinical swabs with suspected viral infection are usually transported in virus transport medium (VMT). During epidemics/pandemics, tampons without VTM would be more suitable for saving space and cost. This study was conducted to verify the applicability of throat swabs without VTM in the diagnosis/screening of enteroviral infections by polymerase chain reaction (PCR) in a volunteer study group. Three different swab types were used in 40 volunteers: swabs with two different tips (cotton- or synthetic-tipped) without VTM and standard synthetic tips with VTM. The swabs were processed immediately or after 12 days of storage at either -80°C or +4°C. The molecular analysis included viral RNA extraction, and combination of reverse transcriptase PCR and nested PCR. Enteroviral RNA was detected in 15% (6/40) of the studied volunteers. When processed immediately, the results for all three swab types were compatible. Swabs without VTM may be used for collection of clinical samples in the diagnosis of suspected enteroviral infections or as potential screening tools for enteroviruses (Tab. 2, Ref. 15). Keywords: enterovirus infection, swab, transport medium, PCR, molecular diagnostics.</p>","PeriodicalId":55328,"journal":{"name":"Bratislava Medical Journal-Bratislavske Lekarske Listy","volume":"125 4","pages":"219-222"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140289714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is it time for anatomists to enter the OT? Their role in clinical anatomy education of residents: A pre-trial survey research among surgeons.","authors":"Arthi Ganapathy, Dibakar Borthakur, Krishna S Patil, Vandana Mehta, Seema Singh, Raghuram Kuppusamy","doi":"10.4149/BLL_2024_69","DOIUrl":"https://doi.org/10.4149/BLL_2024_69","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to assess the perceived need among surgical residents to revisit their anatomical knowledge and evaluate their attitude towards integrating clinical anatomists into surgical residency program curriculum.</p><p><strong>Background: </strong>While medical students learn human anatomy during undergraduate years, the practical application of clinically oriented anatomy becomes vital in surgical specialties. However, this aspect has not been adequately addressed in Indian surgical residency programs.</p><p><strong>Methods: </strong>An 11-item questionnaire, including closed-ended and Likert-scale questions, was administered to 153 surgical residents. Consent was obtained, and responses were collected via Google Forms.</p><p><strong>Results: </strong>Half of the respondents (50%) felt confident in their self-directed anatomy learning, but 87% believed integrating clinical anatomists would enhance their surgical expertise. Additionally, 88% saw value in revisiting cadaveric dissection. Third-year residents showed a significantly higher inclination towards cadaveric dissection. Deficiencies in the curriculum and time constraints were identified as major barriers.</p><p><strong>Conclusion: </strong>The study highlights a perceived need among surgical residents to augment their anatomical knowledge, advocating for the integration of clinical anatomists and cadaveric dissection into training. A collaborative approach, emphasizing both horizontal and vertical integration of anatomy, is recommended to enhance surgical education and practice. (Tab. 4, Fig. 1, Ref. 25).</p>","PeriodicalId":55328,"journal":{"name":"Bratislava Medical Journal-Bratislavske Lekarske Listy","volume":"125 7","pages":"450-456"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141472893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Robotic stereotactic radiosurgery with CyberKnife - brain metastases and fibrinolysis.","authors":"Veselin Popov, Vasko Graklanov, Snezhana Stoencheva, Zhanet Grudeva-Popova","doi":"10.4149/BLL_2023_139","DOIUrl":"https://doi.org/10.4149/BLL_2023_139","url":null,"abstract":"<p><strong>Objectives: </strong>Deviations in haemostasis are found in about 50 % of patients with cancer and up to 90% of those with metastatic disease. Many studies investigate the dynamics of the processes of coagulation and fibrinolysis and their role as a predictor of therapeutic response, early relapse, or metastasis risk.</p><p><strong>Background: </strong>To investigate the serum levels of urokinase plasminogen activator (uPA) in patients with brain metastases treated with robotic stereotactic radiosurgery (SRS) with CyberKnife.</p><p><strong>Material and methods: </strong>Serum levels of urokinase plasminogen activator (uPA) were measured in 66 patients with solid tumours, divided into two groups, with oligometastatic disease and brain metastases. In this prospective longitudinal study, the serum levels of uPA were measured before starting the therapy and at the first, third, and sixth months after patients were irradiated with the CyberKnife system.</p><p><strong>Results: </strong>Analysis of serum uPA levels in the post-treatment period showed a statistically significant decrease between the baseline and the 6 months post-treatment time point in both patient groups. The baseline value of serum uPA in the group with lung cancer decreased by 62.7 %, and in the group with other types of cancer - by 60 %. Despite the significant reduction of serum uPA levels 6 months after the treatment, the levels remained significantly higher in both groups than in healthy controls.</p><p><strong>Conclusion: </strong>Ongoing research on uPA and cancer will enrich our knowledge and expand the possibilities for clinical utilization of the marker in the oncology setting (Tab. 2, Ref. 18).</p>","PeriodicalId":55328,"journal":{"name":"Bratislava Medical Journal-Bratislavske Lekarske Listy","volume":"125 3","pages":"149-152"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139934377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}