Efficacy of sample collection without virus transport medium in suspected enteroviral infections for molecular diagnosis.

IF 1.5 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL
Maria Borsanyiova, Shubhada Bopegamage, Sandor George Vari
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引用次数: 0

Abstract

Clinical swabs with suspected viral infection are usually transported in virus transport medium (VMT). During epidemics/pandemics, tampons without VTM would be more suitable for saving space and cost. This study was conducted to verify the applicability of throat swabs without VTM in the diagnosis/screening of enteroviral infections by polymerase chain reaction (PCR) in a volunteer study group. Three different swab types were used in 40 volunteers: swabs with two different tips (cotton- or synthetic-tipped) without VTM and standard synthetic tips with VTM. The swabs were processed immediately or after 12 days of storage at either -80°C or +4°C. The molecular analysis included viral RNA extraction, and combination of reverse transcriptase PCR and nested PCR. Enteroviral RNA was detected in 15% (6/40) of the studied volunteers. When processed immediately, the results for all three swab types were compatible. Swabs without VTM may be used for collection of clinical samples in the diagnosis of suspected enteroviral infections or as potential screening tools for enteroviruses (Tab. 2, Ref. 15). Keywords: enterovirus infection, swab, transport medium, PCR, molecular diagnostics.

在疑似肠道病毒感染的分子诊断中,不使用病毒运输培养基采集样本的有效性。
疑似病毒感染的临床拭子通常用病毒运输培养基(VMT)运输。在流行病/大流行期间,不含 VTM 的咽拭子更适合用于节省空间和成本。本研究旨在验证不含 VTM 的咽拭子在志愿者研究小组中通过聚合酶链反应(PCR)诊断/筛查肠道病毒感染的适用性。40 名志愿者使用了三种不同类型的咽拭子:不含 VTM 的两种不同吸头(棉花或合成纤维吸头)的咽拭子和含 VTM 的标准合成纤维吸头。拭子立即或在-80°C 或 +4°C下保存 12 天后进行处理。分子分析包括病毒 RNA 提取、逆转录酶 PCR 和巢式 PCR 组合。15% 的志愿者(6/40)检测到了肠道病毒 RNA。在立即处理的情况下,所有三种拭子类型的结果都是一致的。不含 VTM 的拭子可用于采集临床样本以诊断疑似肠道病毒感染或作为潜在的肠道病毒筛查工具(参考文献 15,表 2)。关键词:肠道病毒感染、拭子、运输培养基、PCR、分子诊断。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
2.60
自引率
0.00%
发文量
185
审稿时长
3-8 weeks
期刊介绍: The international biomedical journal - Bratislava Medical Journal – Bratislavske lekarske listy (Bratisl Lek Listy/Bratisl Med J) publishes peer-reviewed articles on all aspects of biomedical sciences, including experimental investigations with clear clinical relevance, original clinical studies and review articles.
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