Clinical & Developmental Immunology最新文献_第7页

A study on clinical and pathologic features in lupus nephritis with mainly IgA deposits and a literature review. 以IgA沉积为主的狼疮性肾炎临床病理特征的研究及文献复习。

Clinical & Developmental Immunology Pub Date : 2013-01-01 Epub Date: 2013-09-24 DOI: 10.1155/2013/289316

Liu Hongyan, Zheng Yi, Dong Bao, Lu Yuewu, Meng Juan

{"title":"A study on clinical and pathologic features in lupus nephritis with mainly IgA deposits and a literature review.","authors":"Liu Hongyan, Zheng Yi, Dong Bao, Lu Yuewu, Meng Juan","doi":"10.1155/2013/289316","DOIUrl":"https://doi.org/10.1155/2013/289316","url":null,"abstract":"Objective: To study the clinical and pathologic features of systemic lupus erythematosus (SLE) that has atypical lupus nephritis (LN) with mainly IgA deposits.Methods: We searched the SLE patients who had nephritis with mainly IgA deposits in our hospital and selected the information including clinical manifestations, laboratory tests, treatments, and prognosis.Results: From January 2009 to June 2012, 5 patients were definitely diagnosed as SLE according to both 1982 and 2009 ACR classification criteria. But renal biopsy showed that all cases had mainly IgA deposits and were free of IgG, C1q, and fibrinogen-related antigen deposits under immunofluorescent microscopy, which did not match with typical LN. There were 2 males and 3 females, aging from 31 to 64 years and with an average of (42.20 ± 13.59) years. The 5 cases had multiple-system involvements, mainly the renal system. Compared to primary IgAN, the atypical LN showed some differences: older than primary IgAN, more women than men, no previous infection history, lower incidence of serum IgA elevation, and ACL positive rate as high as 100%.Conclusion: Nephritis with mainly IgAN deposits, as an atypical LN, may be a special subtype of SLE.","PeriodicalId":55254,"journal":{"name":"Clinical & Developmental Immunology","volume":" ","pages":"289316"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/289316","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40277212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Pediatric inflammatory bowel disease with cytoplasmic staining of antineutrophil cytoplasmic antibodies. 小儿炎症性肠病与抗中性粒细胞胞浆抗体的细胞质染色。

Clinical & Developmental Immunology Pub Date : 2013-01-01 Epub Date: 2013-02-14 DOI: 10.1155/2013/196012

Omar I Saadah, Jamil A Al-Mughales

{"title":"Pediatric inflammatory bowel disease with cytoplasmic staining of antineutrophil cytoplasmic antibodies.","authors":"Omar I Saadah, Jamil A Al-Mughales","doi":"10.1155/2013/196012","DOIUrl":"https://doi.org/10.1155/2013/196012","url":null,"abstract":"Background: It is unusual for the antineutrophil cytoplasmic antibody with cytoplasmic pattern (cANCA) to present in patients with inflammatory bowel disease (IBD) without vasculitis. The purpose of this study was to describe the occurrence and characteristics of pediatrics IBD with cANCA.Methods: A retrospective review of pediatric IBD associated with cANCA serology in patients from King Abdulaziz University Hospital, Saudi Arabia, between September 2002 and February 2012.Results: Out of 131 patients with IBD screened for cANCAs, cANCA was positive in 7 (5.3%) patients of whom 4 had ulcerative colitis and 3 had Crohn's disease. The median age was 8.8 years (2-14.8 years). Six (86%) were males. Of the 7 patients, 5 (71%) were Saudi Arabians and 2 were of Indian ethnicity. The most common symptoms were diarrhea, abdominal pain, weight loss, and rectal bleeding. None had family history or clinical features suggestive of vasculitis involving renal and respiratory systems. No difference in the disease location or severity was observed between cANCA positive and cANCA negative patients apart from male preponderance in cANCA positive patients.Conclusion: The occurrence of cANCA in pediatric IBD is rare. Apart from male preponderance, there were no peculiar characteristics for the cANCA positive patients.","PeriodicalId":55254,"journal":{"name":"Clinical & Developmental Immunology","volume":"2013 ","pages":"196012"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/196012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31295410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The immunologic basis for severe neonatal herpes disease and potential strategies for therapeutic intervention. 新生儿严重疱疹病的免疫学基础和治疗干预的潜在策略。

Clinical & Developmental Immunology Pub Date : 2013-01-01 Epub Date: 2013-03-31 DOI: 10.1155/2013/369172

Soren Gantt, William J Muller

{"title":"The immunologic basis for severe neonatal herpes disease and potential strategies for therapeutic intervention.","authors":"Soren Gantt, William J Muller","doi":"10.1155/2013/369172","DOIUrl":"https://doi.org/10.1155/2013/369172","url":null,"abstract":"Herpes simplex viruses types 1 and 2 (HSV-1 and HSV-2) infect a large proportion of the world's population. Infection is life-long and can cause periodic mucocutaneous symptoms, but it only rarely causes life-threatening disease among immunocompetent children and adults. However, when HSV infection occurs during the neonatal period, viral replication is poorly controlled and a large proportion of infants die or develop disability even with optimal antiviral therapy. Increasingly, specific differences are being elucidated between the immune system of newborns and those of older children and adults, which predispose to severe infections and reflect the transition from fetal to postnatal life. Studies in healthy individuals of different ages, individuals with primary or acquired immunodeficiencies, and animal models have contributed to our understanding of the mechanisms that control HSV infection and how these may be impaired during the neonatal period. This paper outlines our current understanding of innate and adaptive immunity to HSV infection, immunologic differences in early infancy that may account for the manifestations of neonatal HSV infection, and the potential of interventions to augment neonatal immune protection against HSV disease.","PeriodicalId":55254,"journal":{"name":"Clinical & Developmental Immunology","volume":"2013 ","pages":"369172"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/369172","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31468354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 37

Mucins help to avoid alloreactivity at the maternal fetal interface. 粘蛋白有助于避免母体胎儿界面的同种异体反应。

Clinical & Developmental Immunology Pub Date : 2013-01-01 Epub Date: 2013-06-20 DOI: 10.1155/2013/542152

Arnela Redzovic, Gordana Laskarin, Marin Dominovic, Herman Haller, Daniel Rukavina

{"title":"Mucins help to avoid alloreactivity at the maternal fetal interface.","authors":"Arnela Redzovic, Gordana Laskarin, Marin Dominovic, Herman Haller, Daniel Rukavina","doi":"10.1155/2013/542152","DOIUrl":"https://doi.org/10.1155/2013/542152","url":null,"abstract":"During gestation, many different mechanisms act to render the maternal immune system tolerant to semi-allogeneic trophoblast cells of foetal origin, including those mediated via mucins that are expressed during the peri-implantation period in the uterus. Tumour- associated glycoprotein-72 (TAG-72) enhances the already established tolerogenic features of decidual dendritic cells with the inability to progress towards Th1 immune orientation due to lowered interferon (IFN)- γ and interleukin (IL)-15 expression. Mucine 1 (Muc 1) supports alternative activation of decidual macrophages, restricts the proliferation of decidual regulatory CD56(+) bright natural killer (NK) cells, and downregulates their cytotoxic potential, including cytotoxic mediator protein expression. Removing TAG-72 and Muc 1 from the eutopic implantation site likely contributes to better control of trophoblast invasion by T cells and NK cells and appears to have important immunologic advantages for successful implantation, in addition to mechanical advantages. However, these processes may lead to uncontrolled trophoblast growth after implantation, inefficient defence against infection or tumours, and elimination of unwanted immunocompetent cells at the maternal-foetal interface. The use of mucins by tumour cells to affect the local microenvironment in order to avoid the host immune response and to promote local tumour growth, invasion, and metastasis confirms this postulation. ","PeriodicalId":55254,"journal":{"name":"Clinical & Developmental Immunology","volume":"2013 ","pages":"542152"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/542152","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31589161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Human monoclonal antibody-based therapy in the treatment of invasive candidiasis. 人单克隆抗体治疗侵袭性念珠菌病的研究。

Clinical & Developmental Immunology Pub Date : 2013-01-01 Epub Date: 2013-06-26 DOI: 10.1155/2013/403121

Francesca Bugli, Margherita Cacaci, Cecilia Martini, Riccardo Torelli, Brunella Posteraro, Maurizio Sanguinetti, Francesco Paroni Sterbini

{"title":"Human monoclonal antibody-based therapy in the treatment of invasive candidiasis.","authors":"Francesca Bugli, Margherita Cacaci, Cecilia Martini, Riccardo Torelli, Brunella Posteraro, Maurizio Sanguinetti, Francesco Paroni Sterbini","doi":"10.1155/2013/403121","DOIUrl":"https://doi.org/10.1155/2013/403121","url":null,"abstract":"Invasive candidiasis (IC) represents the leading fungal infection of humans causing life-threatening disease in immunosuppressed and neutropenic individuals including also the intensive care unit patients. Despite progress in recent years in drugs development for the treatment of IC, morbidity and mortality rates still remain very high. Historically, cell-mediated immunity and innate immunity are considered to be the most important lines of defense against candidiasis. Nevertheless recent evidence demonstrates that antibodies with defined specificities could act with different degrees showing protection against systemic and mucosal candidiasis. Mycograb is a human recombinant monoclonal antibody against heat shock protein 90 (Hsp90) that was revealed to have synergy when combined with fluconazole, caspofungin, and amphotericin B against a broad spectrum of Candida species. Furthermore, recent studies have established an important role for Hsp90 in mediating Candida resistance to echinocandins, giving to this antibody molecule even more attractive biological properties. In response to the failure of marketing authorization by the CHMP (Committee for Medicinal Products for Human Use) a new formulation of Mycograb, named Mycograb C28Y variant, with an amino acid substitution was developed in recent years. First data on Mycograb C28Y variant indicate that this monoclonal antibody lacked efficacy in a murine candidiasis model. ","PeriodicalId":55254,"journal":{"name":"Clinical & Developmental Immunology","volume":"2013 ","pages":"403121"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/403121","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31600487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 53

The Interplay between the bone and the immune system. 骨骼与免疫系统之间的相互作用

Clinical & Developmental Immunology Pub Date : 2013-01-01 Epub Date: 2013-07-14 DOI: 10.1155/2013/720504

Giorgio Mori, Patrizia D'Amelio, Roberta Faccio, Giacomina Brunetti

{"title":"The Interplay between the bone and the immune system.","authors":"Giorgio Mori, Patrizia D'Amelio, Roberta Faccio, Giacomina Brunetti","doi":"10.1155/2013/720504","DOIUrl":"10.1155/2013/720504","url":null,"abstract":"In the last two decades, numerous scientists have highlighted the interactions between bone and immune cells as well as their overlapping regulatory mechanisms. For example, osteoclasts, the bone-resorbing cells, are derived from the same myeloid precursor cells that give rise to macrophages and myeloid dendritic cells. On the other hand, osteoblasts, the bone-forming cells, regulate hematopoietic stem cell niches from which all blood and immune cells are derived. Furthermore, many of the soluble mediators of immune cells, including cytokines and growth factors, regulate the activities of osteoblasts and osteoclasts. This increased recognition of the complex interactions between the immune system and bone led to the development of the interdisciplinary osteoimmunology field. Research in this field has great potential to provide a better understanding of the pathogenesis of several diseases affecting both the bone and immune systems, thus providing the molecular basis for novel therapeutic strategies. In these review, we reported the latest findings about the reciprocal regulation of bone and immune cells. ","PeriodicalId":55254,"journal":{"name":"Clinical & Developmental Immunology","volume":"2013 ","pages":"720504"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3725924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31648768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of increased blood levels of GITR and GITRL with disease severity in patients with primary Sjögren's syndrome. 原发性Sjögren综合征患者血液中GITR和GITRL水平升高与疾病严重程度的相关性

Clinical & Developmental Immunology Pub Date : 2013-01-01 Epub Date: 2013-07-07 DOI: 10.1155/2013/340751

Xiaoxia Gan, Xiaoke Feng, Lei Gu, Wenfeng Tan, Xiaoxuan Sun, Chengyin Lv, Miaojia Zhang

{"title":"Correlation of increased blood levels of GITR and GITRL with disease severity in patients with primary Sjögren's syndrome.","authors":"Xiaoxia Gan, Xiaoke Feng, Lei Gu, Wenfeng Tan, Xiaoxuan Sun, Chengyin Lv, Miaojia Zhang","doi":"10.1155/2013/340751","DOIUrl":"https://doi.org/10.1155/2013/340751","url":null,"abstract":"Glucocorticoid-induced tumor necrosis factor receptor family-related protein (GITR) is a type I transmembrane protein belonging to the TNFR superfamily. After activated by its ligand GITRL, GITR could influence the activity of effector and regulatory T cells, participating in the development of several autoimmune and inflammatory diseases included rheumatoid arthritis and autoimmune thyroid disease. We previously reported that serum GITRL levels are increased in systemic lupus erythematosus (SLE) patients compared with healthy controls (HC). Here, we tested serum soluble GITR (sGITR) and GITRL levels in 41 primary Sjögren's syndrome (pSS) patients and 29 HC by ELISA and correlated sGITR and GITRL levels with clinical and laboratory variables. GITR and GITRL expression in labial salivary glands was detected by immunohistochemistry. pSS patients had significantly increased serum levels of sGITR and GITRL compared with controls (GITR: 5.66 ± 3.56 ng/mL versus 0.50 ± 0.31 ng/mL; P < 0.0001; GITRL: 6.17 ± 7.10 ng/mL versus 0.36 ± 0.28 ng/mL; P < 0.0001). Serum sGITR and GITRL levels were positively correlated with IgG (GITRL: r = 0.6084, P < 0.0001; sGITR: r = 0.6820, P < 0.0001) and ESR (GITRL: r = 0.8315, P < 0.0001; sGITR: r = 0.7448, P < 0.0001). Moreover, GITR and GITRL are readily detected in the lymphocytic foci and periductal areas of the LSGs. In contrast, the LSGs of HC subjects did not express GITR or GITRL. Our findings indicate the possible involvement of GITR-GITRL pathway in the pathogenesis of pSS. Further studies may facilitate the development of targeting this molecule pathway for the treatment of pSS. ","PeriodicalId":55254,"journal":{"name":"Clinical & Developmental Immunology","volume":"2013 ","pages":"340751"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/340751","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31648831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Tumor protective activity of CD4+ but not of CD8+ T cells in DNA-vaccinated mice challenged with bcr-abl-transformed cells. 用bcr-abl转化细胞攻击dna疫苗小鼠，CD4+而CD8+ T细胞的肿瘤保护活性。

Clinical & Developmental Immunology Pub Date : 2013-01-01 Epub Date: 2013-11-21 DOI: 10.1155/2013/923107

Martina Petráčková, Vincent Lučanský, Vladimír Vonka

{"title":"Tumor protective activity of CD4+ but not of CD8+ T cells in DNA-vaccinated mice challenged with bcr-abl-transformed cells.","authors":"Martina Petráčková, Vincent Lučanský, Vladimír Vonka","doi":"10.1155/2013/923107","DOIUrl":"https://doi.org/10.1155/2013/923107","url":null,"abstract":"In the recent past, it has repeatedly been reported that CD4 cells play an important role in the immunology of chronic myeloid leukaemia. It was therefore of interest to test their activity in an animal model using bcr-abl-transformed cells. BALB/c mice were four times immunized with a DNA vaccine carrying the bcr-abl fusion gene. Two weeks after the last vaccine dose, the animals were challenged with syngeneic bcr-abl-transformed 12B1 cells which form solid tumors after subcutaneous administration. At the time of challenge, animals were treated with antibodies against the CD8+ T cells or CD4+ T cells. The efficacy of the depletion was monitored and found highly effective. All nonimmunized animals developed tumors. All animals untreated with the antibodies as well as those in which CD8+ T cells had been depleted, were fully protected against the challenge. On the other hand, almost all mice treated with anti-CD4+ antibody developed tumors. These results strongly suggested that the CD4+ T cells acted as effectors in the present system. ","PeriodicalId":55254,"journal":{"name":"Clinical & Developmental Immunology","volume":"2013 ","pages":"923107"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/923107","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31963990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

APACHE IV is superior to MELD scoring system in predicting prognosis in patients after orthotopic liver transplantation. APACHE IV在预测原位肝移植术后患者预后方面优于MELD评分系统。

Clinical & Developmental Immunology Pub Date : 2013-01-01 Epub Date: 2013-11-18 DOI: 10.1155/2013/809847

Yueyun Hu, Xianling Zhang, Yuan Liu, Jun Yan, Tiehua Li, Ailing Hu

{"title":"APACHE IV is superior to MELD scoring system in predicting prognosis in patients after orthotopic liver transplantation.","authors":"Yueyun Hu, Xianling Zhang, Yuan Liu, Jun Yan, Tiehua Li, Ailing Hu","doi":"10.1155/2013/809847","DOIUrl":"https://doi.org/10.1155/2013/809847","url":null,"abstract":"This study aims to compare the efficiency of APACHE IV with that of MELD scoring system for prediction of the risk of mortality risk after orthotopic liver transplantation (OLT). A retrospective cohort study was performed based on a total of 195 patients admitted to the ICU after orthotopic liver transplantation (OLT) between February 2006 and July 2009 in Guangzhou, China. APACHE IV and MELD scoring systems were used to predict the postoperative mortality after OLT. The area under the receiver operating characteristic curve (AUC) and the Hosmer-Lemeshow C statistic were used to assess the discrimination and calibration of APACHE IV and MELD, respectively. Twenty-seven patients died during hospitalization with a mortality rate of 13.8%. The mean scores of APACHE IV and MELD were 42.32 ± 21.95 and 18.09 ± 10.55, respectively, and APACHE IV showed better discrimination than MELD; the areas under the receiver operating characteristic curve for APACHE IV and MELD were 0.937 and 0.694 (P < 0.05 for both models), which indicated that the prognostic value of APACHE IV was relatively high. Both models were well-calibrated (The Hosmer-Lemeshow C statistics were 1.568 and 6.818 for APACHE IV and MELD, resp.; P > 0.05 for both). The respective Youden indexes of APACHE IV, MELD, and combination of APACHE IV with MELD were 0.763, 0.430, and 0.545. The prognostic value of APACHE IV is high but still underestimates the overall hospital mortality, while the prognostic value of MELD is poor. The function of the APACHE IV is, thus, better than that of the MELD. ","PeriodicalId":55254,"journal":{"name":"Clinical & Developmental Immunology","volume":"2013 ","pages":"809847"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/809847","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31964046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

A new ELISA for dermatomyositis autoantibodies: rapid introduction of autoantigen cDNA to recombinant assays for autoantibody measurement. 一种新的皮肌炎自身抗体ELISA检测方法:将自身抗原cDNA快速引入重组法检测自身抗体。

Clinical & Developmental Immunology Pub Date : 2013-01-01 Epub Date: 2013-12-12 DOI: 10.1155/2013/856815

Yoshinao Muro, Kazumitsu Sugiura, Masashi Akiyama

{"title":"A new ELISA for dermatomyositis autoantibodies: rapid introduction of autoantigen cDNA to recombinant assays for autoantibody measurement.","authors":"Yoshinao Muro, Kazumitsu Sugiura, Masashi Akiyama","doi":"10.1155/2013/856815","DOIUrl":"https://doi.org/10.1155/2013/856815","url":null,"abstract":"Advances in immunology, biochemistry, and molecular biology have enabled the development of a number of assays for measuring autoantibodies. ELISA has been widely used, because it can deal with relatively large numbers of serum samples more quickly than other immunologic methods, such as immunoblotting and immunoprecipitation. Recombinant autoantigens, which are generally produced in E. coli using the relevant cloned cDNA, are necessary for ELISA. Conventional clinical ELISA tests are limited in their ability to purify proteins free of bacterial contaminants, and the process is labor intensive. We recently developed new ELISA tests that utilize simple in vitro transcription and translation labeling of autoantigens in order to measure dermatomyositis-(DM-) specific autoantibodies, including autoantibodies to Mi-2, MDA5, NXP-2, TIF1-α, and TIF1-γ. This method may allow for the rapid conversion of cDNAs to a chemiluminescent ELISA to detect autoantibodies that are found not only in DM but also in other autoimmune diseases.","PeriodicalId":55254,"journal":{"name":"Clinical & Developmental Immunology","volume":"2013 ","pages":"856815"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/856815","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32022269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32