Cytopathology最新文献

{"title":"Proposal for Clinical Management of Nodules Diagnosed as Atypia of Undetermined Significance via Thyroid Fine-Needle Aspiration Cytology in the Absence of Molecular Testing","authors":"Ayana Suzuki,&nbsp;Mitsuyoshi Hirokawa,&nbsp;Makoto Kawakami,&nbsp;Takumi Kudo,&nbsp;Akira Miyauchi,&nbsp;Takashi Akamizu","doi":"10.1111/cyt.13467","DOIUrl":"10.1111/cyt.13467","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Molecular testing is recommended for risk stratification of atypia of undetermined significance (AUS) nodules in the USA; however, it is not routinely performed in some countries owing to limited availability and affordability. Here, we propose a risk stratification algorithm for AUS nodules when molecular testing is unavailable.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We examined 304 (4.3%) AUS nodules among 7073 thyroid fine-needle aspiration cytology specimens examined at Kuma Hospital from January 2020 to December 2020. Clinical data were obtained from the medical records of Kuma Hospital.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>AUS with nuclear atypia and AUS-other each accounted for half of the total AUS nodules. The repeat aspiration rate was 19.7%; 61.7% of the nodules were reclassified as benign or malignant upon repeat aspiration. Resection rate and overall risk of malignancy (ROM) were 32.6% and 12.8%, respectively. Architectural atypia showed the lowest (1.1%) overall ROM in the AUS nodules. For AUS with nuclear atypia, nodules ≤ 10 mm in size showed significantly lower overall ROM than those of &gt; 10 mm, and nodules with ultrasonographically low suspicion showed significantly lower overall ROM than those with intermediate to high suspicion. AUS nodules with atypical lymphoid cells, possible medullary thyroid carcinoma, or possible parathyroid lesion were confirmed using flow cytometry, biochemical testing using needle washout fluid or immunocytochemistry, respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our proposed clinical management algorithm for each subdivision according to cytological findings, based on repeat aspiration rates, ROM, ultrasound findings and results of ancillary tests except for molecular testing, should be useful for the clinical management of AUS nodules.</p>\u0000 </section>\u0000 </div>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 2","pages":"115-122"},"PeriodicalIF":1.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cyt.13467","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Insighhts Into JC Virus–Infected Glial Cells: Intraoperative Cytopathological Analysis of Dot-Shaped Inclusions Associated With PML-NBs","authors":"Nobuko Ujihira,&nbsp;Hideki Doi,&nbsp;Yukiko Shishido-Hara","doi":"10.1111/cyt.13469","DOIUrl":"10.1111/cyt.13469","url":null,"abstract":"<p>This is the first case report describing the diagnostic value of dot-shaped inclusions associated with promyelocytic leukaemia nuclear bodies (PML-NBs) to define JC virus–infected glial cells in an intraoperative cytopathological diagnosis for progressive multifocal leukoencephalopathy (PML).\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 3","pages":"281-284"},"PeriodicalIF":1.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cyt.13469","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142959038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the Transformation Process of Thyroid Fine-Needle Aspiration Liquid-Based Cytology to Whole-Slide Image","authors":"Yuanyuan Lei,&nbsp;Dongcun Wang,&nbsp;Yanlin Wen,&nbsp;Jinhui Liu,&nbsp;Jian Cao","doi":"10.1111/cyt.13468","DOIUrl":"10.1111/cyt.13468","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Analyse and summarise the reasons for failure in the digital acquisition of thyroid liquid-based cytology (LBC) slides and the technical challenges, and explore methods to obtain reliable and reproducible whole digital slide images for clinical thyroid cytology.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method</h3>\u0000 \u0000 <p>Use the glass slide scanning imaging system to acquire whole-slide image (WSI) of thyroid LBC in sdpc format through different. Statistical analysis was conducted on the different acquisition methods, the quality of the glass slides, clinical and pathological characteristics of the case, TBSRTC grading and the quality of WSI.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The WSI obtained by different scanning methods showed a high level of consistency in quality (<i>W</i> = 0.325, <i>p</i> &lt; 0.001), especially between fully automatic scanning with different focus densities (<i>W</i> = 0.9, <i>p</i> &lt; 0.001). A total of 2114 images were obtained through different methods of multi-layer fusion and multi-point focusing scanning, with scan success rates of 100.0%, 100.0%, 100.0% and 23.6%, respectively. The correlation between the quality of thyroid LBC glass slides and the image quality of thyroid LBC WSI was statistically significant (<i>p</i> &lt; 0.001). The correlation between TBSRTC grading and the quality of thyroid LBC digital WSI was statistically significant (<i>p</i> &lt; 0.001).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Although the quality of glass slides has a significant impact, the success rate and image quality of malignant tumour scanning are both high. Overall, the risk of missed diagnosis of malignant tumours is low. In the future, we also need to improve the performance and algorithm of the scanner in cases of sparse cells.</p>\u0000 </section>\u0000 </div>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 2","pages":"106-114"},"PeriodicalIF":1.2,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cyt.13468","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142959052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytological Features of Sialoblastoma: A Case Report With Summary of Prior Published Cases","authors":"Firdous Wasim Sk,&nbsp;Debalina Karmakar,&nbsp;Urvee Sarkar,&nbsp;Dipak Ghosh,&nbsp;Uttara Chatterjee","doi":"10.1111/cyt.13466","DOIUrl":"10.1111/cyt.13466","url":null,"abstract":"<p>Cytological diagnosis of sialoblastoma can be challenging. This report describes describe the cytological findings of sialoblastoma in an infant along with its cytological differentials and a summary of prior published cases. Cellular smears composed of small round basaloid cells with occasional ductal formation were the main features.</p>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 2","pages":"175-179"},"PeriodicalIF":1.2,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytology of an Axillary Mass: A Rare Case","authors":"Sanjana S James,&nbsp;Amit Kumar Adhya","doi":"10.1111/cyt.13465","DOIUrl":"10.1111/cyt.13465","url":null,"abstract":"<p>This is an interesting case of rhabdomyosarcoma, presenting at an unusual site and diagnosed on FNAC. The smears depict the typical malignant small round cell morphology and tigroid background. It illustrates the role of cell block and immunohistochemistry as important ancillary adjuncts to morphology.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 2","pages":"204-207"},"PeriodicalIF":1.2,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142848530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling Diagnostic Clues of NTRK-Rearranged Spindle Cell Neoplasms in Fine-Needle Aspiration Specimens","authors":"Yilin Xie,&nbsp;Diwei Zhou,&nbsp;Li Peng,&nbsp;Xiaona Chang,&nbsp;Jingmin Zhong","doi":"10.1111/cyt.13464","DOIUrl":"10.1111/cyt.13464","url":null,"abstract":"<div>\u0000 \u0000 <p><i>NTRK</i> (neurotropic tropomyosin receptor kinase)-rearranged spindle cell tumours represent a rare group of molecularly defined soft tissue neoplasms. These tumours, excluding infantile fibrosarcomas, are characterised by <i>NTRK</i> gene rearrangements and exhibit a range of histomorphologies, including spindle, epithelioid or rhabdoid cells with invasive growth. Their prognosis correlates with histological grade, and surgical resection is the primary treatment. The abnormally expressed oncogenic receptor tyrosine kinases TRK-A, TRK-B and TRK-C in this tumour have been shown to be therapeutical targetable, which may improve patient prognosis. We report a 17-year-old male patient presenting with a left axillary mass. Both fine-needle aspiration cytology (FNAC) and surgical resection specimens were submitted for examination. Comprehensive analysis of cytomorphology, immunohistochemical staining results and next-generation sequencing (NGS) data led to a final diagnosis of <i>NTRK</i>-rearranged spindle cell tumour. By comparing cytological and histological morphologies, we identified diagnostic clues in cytological specimens. <i>NTRK</i>-rearranged spindle cell tumours' definitive diagnosis enables targeted therapy. Fine-needle aspiration cytology, being minimally invasive, offers the potential for earlier and more definitive diagnoses, thereby improving patient treatment options.</p>\u0000 </div>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 2","pages":"170-174"},"PeriodicalIF":1.2,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142819963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reporting Bone Cytopathology—A Proposal Based on a Single Tertiary Centre Experience","authors":"Jan Köster,&nbsp;Camila Bedeschi Rego De Mattos,&nbsp;Henryk A. Domanski","doi":"10.1111/cyt.13462","DOIUrl":"10.1111/cyt.13462","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Fine-needle aspiration cytology (FNAC) from bone lesions has been proven to be a useful diagnostic tool but lacks standardisation. The aim of the study was to evaluate the diagnostic utility of FNAC as a basis to propose and test a reporting system for bone reporting cytopathology.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This retrospective study is based on patients with bone lesions, that were approached by cytology at Skåne University Hospital, Sweden between 2015 and 2023. The diagnostic performance was measured by sensitivity, specificity and accuracy analyses. All diagnoses were then distributed in six categories: (I) Non-diagnostic, (II) Benign, (III) Atypia, (IV) Bone neoplasm of uncertain significance, (V) Suspicious for malignancy and (VI) Malignant. The risk of malignancy (ROM) in each category was calculated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The final cohort consisted of 721 cases. Bone cytology was able to differentiate between benign and malignant lesion with a sensitivity of 89% and a specificity of 99%. The overall diagnostic accuracy was 65% but varied significantly among different types of lesions. Within the tested diagnostic categories, the ROM was (I) 48%, (II) 6.7%, (III) 69%, (IV) 28%, (V) 93% and (VI) 100%.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>FNAC from bone lesions is a sensitive and specific diagnostic tool with high diagnostic accuracy among various tumour types. This study provides valuable insights for the development of a standardised reporting system for bone cytopathology.</p>\u0000 </section>\u0000 </div>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 2","pages":"123-139"},"PeriodicalIF":1.2,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cyt.13462","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142803560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of AI-Assisted in Digital Cervical Cytology Training: A Comparative Study","authors":"Yihui Yang,&nbsp;Dongyi Xian,&nbsp;Lihua Yu,&nbsp;Yanqing Kong,&nbsp;Huaisheng Lv,&nbsp;Liujing Huang,&nbsp;Kai Liu,&nbsp;Hao Zhang,&nbsp;Weiwei Wei,&nbsp;Hongping Tang","doi":"10.1111/cyt.13461","DOIUrl":"10.1111/cyt.13461","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study aimed to investigate the supporting role of artificial intelligence (AI) in digital cervical cytology training.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A total of 104 trainees completed both manual reading and AI-assisted reading tests following the AI-assisted digital training regimen. The interpretation scores and the testing time in different groups were compared. Also, the consistency, sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of diagnoses were further analysed through the confusion matrix and inconsistency evaluation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The mean interpretation scores were significantly higher in the AI-assisted group compared with the manual reading group (81.97 ± 16.670 vs. 67.98 ± 21.469, <i>p</i> &lt; 0.001), accompanied by a reduction in mean interpretation time (32.13 ± 11.740 min vs. 11.36 ± 4.782 min, <i>p</i> &lt; 0.001). The proportion of trainees' results with complete consistence (Category O) were improved from 0.645 to 0.803 and the averaged pairwise <i>κ</i> scores were improved from 0.535 (moderate) to 0.731 (good) with AI assistance. The number of correct answers, accuracies, sensitivities, specificities, PPV, NPV and <i>κ</i> scores of most class-specific diagnoses (NILM, Fungi, HSV, LSIL, HSIL, AIS, AC) also improved with AI assistance. Moreover, 97.8% (89/91) of trainees reported substantial improvement in cervical cytology interpretation ability, and all participants (100%, 91/91) expressed a strong willingness to integrate AI-assisted diagnosis into their future practice.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The utilisation of an AI-assisted digital cervical cytology training platform positively impacted trainee performance and received high satisfaction and acceptance among clinicians, suggesting its potential as a valuable adjunct to medical education.</p>\u0000 </section>\u0000 </div>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 2","pages":"156-164"},"PeriodicalIF":1.2,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142796600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytology of Bilateral Lymph Nodal Masses in a Young Adult","authors":"Rasheeda Mohamedali,&nbsp;Uma Handa,&nbsp;Monica Gupta","doi":"10.1111/cyt.13460","DOIUrl":"10.1111/cyt.13460","url":null,"abstract":"<p>Rosai Dorfman Destombes disease, also known as sinus histiocytosis with massive lymphadenopathy, is a rare non-neoplastic idiopathic disorder characterised by histiocytic proliferation with evidence of emperipolesis. They present with a varied spectrum of clinical manifestations and cytomorphological features, posing diagnostic challenges and therapeutic dilemmas.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 2","pages":"208-210"},"PeriodicalIF":1.2,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"‘Insufficient’/‘Inadequate’/‘Non-Diagnostic’ Category of the WHO Reporting System for Lung Cytopathology, 2022: A Survey About Use in Practice","authors":"Jesus Vega-Gonzalez,&nbsp;Montserrat de la Torre Serrano,&nbsp;Ana María Colino Gallardo,&nbsp;María Reyes Bergillos Giménez,&nbsp;Desiré Hernández Martínez,&nbsp;Alejandro García Egido,&nbsp;Miguel de Hita Castillo,&nbsp;Ignacio Lample Brusel,&nbsp;Elvira Oteo Cerrato,&nbsp;Sara Platero Sanz,&nbsp;Lone Nielsen,&nbsp;Mª Jesús Fernández-Aceñero","doi":"10.1111/cyt.13463","DOIUrl":"10.1111/cyt.13463","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>The new ‘WHO Reporting System for Lung Cytopathology’ (2022) includes an ‘Insufficient’/‘Inadequate’/‘Non-diagnostic’ category. Although they recommend that each laboratory should choose one term to report this diagnostic category, they do not recommend one term over the others. Our aim was to evaluate the use of these three terms in pulmonary FNACs among Spanish pathologists.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A web-based survey among Spanish pathologists using Google Forms was performed. It included 14 yes/no and multiple-choice questions, the latter with an open-text option where it was possible to give a different answer from the ones given. Some questions inquired about their daily practice routine whilst others proposed different cytological examples with the intention of knowing how they would report those particular cases.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Twenty-eight participants (18 cytopathologists, two general pathologists and seven residents) from 17 different Spanish hospitals completed the survey. Most of the respondents use more than one term of this diagnostic category, and there is a lack of reproducibility on the criteria to use one specific term.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>There is a need to clearly define this category to reduce variability between pathologists.</p>\u0000 </section>\u0000 </div>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 2","pages":"165-169"},"PeriodicalIF":1.2,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142781940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}