Hybridoma最新文献_第6页

Monoclonal and polyclonal antibodies against human ferritin, a nonspecific tumor marker. 抗人铁蛋白(非特异性肿瘤标志物)单克隆和多克隆抗体。

Hybridoma Pub Date : 2001-02-01 DOI: 10.1089/027245701300060481

Shu-Fen Chou, Chien-Yuan Chen

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引用次数: 9

Establishment of hybridoma cell lines producing monoclonal antibodies against hepatitis B virus surface antigens (a, d, and r) and development of sensitive ELISA diagnostic test. 产生乙型肝炎病毒表面抗原(a、d、r)单克隆抗体的杂交瘤细胞系的建立及ELISA敏感诊断试验的研制

Hybridoma Pub Date : 2001-02-01 DOI: 10.1089/027245701300060445

X. Tang, X. Zhang, H. Xu

{"title":"Establishment of hybridoma cell lines producing monoclonal antibodies against hepatitis B virus surface antigens (a, d, and r) and development of sensitive ELISA diagnostic test.","authors":"X. Tang, X. Zhang, H. Xu","doi":"10.1089/027245701300060445","DOIUrl":"https://doi.org/10.1089/027245701300060445","url":null,"abstract":"A new treble-coated enzyme-linked immunoadsorbent assay (ELISA) kit of detecting Hepatitis B virus (HBV) surface antigen subtypes a, d and r (HBsAg-a, -d, -r) was developed by using four established hybridoma cell lines, of which two specifically secrete monoclonal antibodies (MAbs) against HBsAg-a (anti-HBsAg-a), one against -d (anti-HBsAg-d), and one against -r (anti-HBsAg-r). The approach of hybridoma cell lines' establishment were by fusing myeloma cells (SP2/0) with splenocytes from BALB/c mice immunized with a mixture of HBsAg-a, -d, -r. The ascitic MAb productivity of the four cell lines was at the titres of 1:10(6)-1:10(8). A treble-coated ELISA based HBV diagnostic kit was developed for detecting all of the three responding subtypes of HBsAgs. A 96-well ELISA microplate was coated with anti-HBSAg-a, -d, -r at a ratio of 3: 1: 0.5, with a horseradish peroxidase (HRP) conjugated anti-HBsAg-a as the labelled antibody. For clinical application, the new developed diagnostic kit detected HBsAgs of adr, adw, ayr, and ayw at a rate of lower than 0.25, 0.25, 0.5, and 0.5 ng/mL, respectively. Results indicated that this kit was more rapid and sensitive than that other current ELISA-based kits coated with a single MAb (e.g., anti-HBsAg-a).","PeriodicalId":55044,"journal":{"name":"Hybridoma","volume":"20 1 1","pages":"47-52"},"PeriodicalIF":0.0,"publicationDate":"2001-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/027245701300060445","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60498649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

(a)20 Anti-human Apolipoprotein(a) Protease Domain. (a)20抗人载脂蛋白(a)蛋白酶结构域。

Hybridoma Pub Date : 2001-01-01 DOI: 10.1089/027245701300060508

J. Kwak

引用次数: 0

FMU1, FMU2, FMU3, FMU4, FMU5, FMU6, and FMU7 Anti-CD226 (PTA1). FMU1、FMU2、FMU3、FMU4、FMU5、FMU6和FMU7抗CD226（PTA1）。

Hybridoma Pub Date : 2001-01-01 DOI: 10.1089/027245701300060562

引用次数: 0

1C2 Anti-Platelet Glycoprotein V. 抗血小板糖蛋白V;

Hybridoma Pub Date : 2001-01-01 DOI: 10.1089/027245701300060661

引用次数: 0

(a)23 Anti-human Apolipoprotein(a) Kringle V Domain. (a)23抗人载脂蛋白(a) Kringle V结构域。