Hybridoma最新文献_第5页

Characterization of a monoclonal antibody against neopterin using an enzyme-linked immunosorbent assay with penicillinase as label. 用青霉素酶为标记的酶联免疫吸附法鉴定抗新蝶呤单克隆抗体。

Hybridoma Pub Date : 2001-04-01 DOI: 10.1089/02724570152057616

M. Malakaneh, M. Rasaee, F. Rahbarizadeh, R. Madani, M. Forozandeh, K. Khabiri, M. H. Alimohammadian

引用次数: 2

Immunological characterization of a mucin-associated protein from hamster tracheal epithelial cell culture. 仓鼠气管上皮细胞培养黏液相关蛋白的免疫学特性。

Hybridoma Pub Date : 2001-04-01 DOI: 10.1089/02724570152057625

Y. Park, C. Shin, W. J. Lee, M. Jo, J. Ryu, E. Choi, K. C. Kim, K. Ko

{"title":"Immunological characterization of a mucin-associated protein from hamster tracheal epithelial cell culture.","authors":"Y. Park, C. Shin, W. J. Lee, M. Jo, J. Ryu, E. Choi, K. C. Kim, K. Ko","doi":"10.1089/02724570152057625","DOIUrl":"https://doi.org/10.1089/02724570152057625","url":null,"abstract":"Airway mucins are high molecular mass (>10(6) dalton) glycoproteins with various types of associated molecules including glycoproteins, lipoproteins, and lipids. The study of mucin-associated proteins is limited largely due to the lack of specific probes. In this study, we produced a monoclonal antibody, MAbHT10, against a 190-kDa mucin associated-protein by immunizing mice with hamster airway mucin purified in nondissociative condition. Using HT10, the 190-kDa mucin-associated protein was characterized immunologically. The 190-kDa mucin-associated protein is glycoprotein and HT10 recognized carbohydrate containing portion of the protein. The association of 190-kDa protein with mucin is strong enough that heat and detergent treatment is required to dissociate it from mucin as evidenced by gel filtration chromatography, Western blot, enzyme-linked immunoadsorbent assay (ELISA), and co-immunoprecipitation. The expression of the 190-kDa protein is increased with the development of hamster tracheal epithelial cells in culture, but showed differences with the pattern of the regulation of mucin expression. Adenosine triphosphate (ATP), a known strong mucin secretagogue, dose-dependently increased mucin release but caused only marginal increase in the release of the 190-kDa protein. The MAb should be useful in the structural and functional analysis of the 190-kDa mucin-associated proteins in physiological and pathological situations such as chronic airway diseases.","PeriodicalId":55044,"journal":{"name":"Hybridoma","volume":"20 2 1","pages":"123-9"},"PeriodicalIF":0.0,"publicationDate":"2001-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/02724570152057625","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60498758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Differential expression and regulation of CD6 on T-cell subsets revealed by monoclonal antibody (MAb) CH11. 单克隆抗体(MAb) CH11揭示CD6在t细胞亚群上的差异表达和调控

Hybridoma Pub Date : 2001-04-01 DOI: 10.1089/02724570152057562

I. Bank, R. Dardik, V. Lévy, I. Goldstein, J. Shoham

{"title":"Differential expression and regulation of CD6 on T-cell subsets revealed by monoclonal antibody (MAb) CH11.","authors":"I. Bank, R. Dardik, V. Lévy, I. Goldstein, J. Shoham","doi":"10.1089/02724570152057562","DOIUrl":"https://doi.org/10.1089/02724570152057562","url":null,"abstract":"A monoclonal antibody (MAb), CH11, was developed by immunizing mice with CD4+ gammadelta T-cell receptor (TCR)+ cells. It recognized an antigen expressed in the surface membrane of T-cell lines, but not of U937, lymphoblastoid B cells (LBC), K562, Raji or Daudi cells, indicating selectivity for the T-cell lineage. In addition, it labelled 70-80% of normal peripheral blood mononuclear cells (PBMC), with high expression on the erythrocyte rosetting (E+) fraction, and low/absent expression on E- cells. However, CD4+ T cells expressed higher levels of reactivity than CD8+ or gammadelta+ T-cell receptor (TCR)+ lymphocytes in PB. Furthermore, in 7 of 10 individuals tested, 7.34+/-3.88% of unselected PBMC were CH11- CD3+ and were relatively enriched in CD8+ and in gammadelta TCR+-cells. In addition, thymic gammadelta T cells, and gammadelta lymphoproliferations from two patients were nonreactive or weakly reactive with the MAb. Activation of E+ cells with phorbol-12-myristate-13-acetate (PMA) enhanced CH11 expression uniformly, whereas activation with phytohemagglutinin (PHA) selectively down-regulated expression of the antigen on the CD8+ subset. In Western blots performed in nonreducing (NR) conditions, MAb CH11 detected a 100 kDa molecule in PBMC and Jurkat T-cell lysates. Preincubation of T cells with MAb CH11 specifically abrogated their subsequent reactivity with MAb to CD6, suggesting that MAb CH11 is recognizing an epitope of CD6. Given its function as a receptor for ligands on thymic epithelium, activated leukocytes and synoviocytes, this newly defined heterogeneity of expression and regulation of the CD6 molecule on subsets of T cells may help determine their functional repertoire in vivo.","PeriodicalId":55044,"journal":{"name":"Hybridoma","volume":"20 2 1","pages":"75-84"},"PeriodicalIF":0.0,"publicationDate":"2001-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/02724570152057562","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60498750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Production of monoclonal antibodies against mammalian Ring1B proteins. 哺乳动物环状1b蛋白单克隆抗体的制备。

Hybridoma Pub Date : 2001-02-01 DOI: 10.1089/027245701300060427

T. Atsuta, S. Fujimura, H. Moriya, M. Vidal, T. Akasaka, H. Koseki

引用次数: 65

Development of a sandwich ELISA test for arginase measurement based on monoclonal antibodies. 基于单克隆抗体的精氨酸酶夹心ELISA检测方法的建立。

Hybridoma Pub Date : 2001-02-01 DOI: 10.1089/027245701300060463

L. Huang, H. W. Liu, K. Chang

引用次数: 6

Immune response to progesterone immobilized on Cu2+-induced amphifilic polyelectrolyte-protein complex: antigen specificity and affinity of hybridoma clones. Cu2+诱导的双filic polyelectrolyte-protein复合物对黄体酮固定化的免疫应答:杂交瘤克隆的抗原特异性和亲和力。

Hybridoma Pub Date : 2001-02-01 DOI: 10.1089/027245701300060346

F. Yücel, B. Çirakoğlu, Z. Mustafaeva, M. Mustafaev

引用次数: 5

Development of a monoclonal antibody specific to a recombinant envelope protein from dengue virus type 4 expressed in Pichia pastoris. 在毕赤酵母中表达的登革病毒4型重组包膜蛋白单克隆抗体的制备。

Hybridoma Pub Date : 2001-02-01 DOI: 10.1089/027245701300060409

M. Pupo-Antúnez, R. Rodríguez, M. Álvarez, N. Amin, H. Rodríguez, A. Otero, G. Guzmán

引用次数: 10

A monoclonal antibody specific for human thymidine kinase 1. 人胸苷激酶1特异性单克隆抗体。

Hybridoma Pub Date : 2001-02-01 DOI: 10.1089/027245701300060382

F. Zhang, X. Shao, H. Li, J. G. Robison, B. K. Murray, K. O’Neill

引用次数: 21

Detection of pET-vector encoded, recombinant S-tagged proteins using the monoclonal antibody ATOM-2. 使用单克隆抗体ATOM-2检测pet载体编码的重组s标记蛋白。

Hybridoma Pub Date : 2001-02-01 DOI: 10.1089/027245701300060364

J. H. Park, S. Na, H. H. Lee, Y. J. Lee, K. L. Kim

{"title":"Detection of pET-vector encoded, recombinant S-tagged proteins using the monoclonal antibody ATOM-2.","authors":"J. H. Park, S. Na, H. H. Lee, Y. J. Lee, K. L. Kim","doi":"10.1089/027245701300060364","DOIUrl":"https://doi.org/10.1089/027245701300060364","url":null,"abstract":"The 15-meric S-tag is a truncated form of the S-peptide, which builds together with the 103 amino acid large S-protein the whole ribonuclease S-protein. Its small size and excessive solubility have made the S-tag an excellent fusion partner in the production of recombinant proteins, and a large variety of applications have been reported using the S-tag as a carrier. While S-tagged proteins were mostly detected and analyzed so far by use of their affinity to S-proteins, monoclonal antibodies (MAbs) for this tag have been not available. The generation of antibodies specific for S-tagged proteins is expected to broaden the range of applications of such S-tag fused recombinant proteins, and in this context, a novel MAb termed ATOM-2 was generated that specifically binds S-tagged proteins, which have been expressed using pET-vectors. Antigen specificity of ATOM-2 was confirmed in Western blot and enzyme-linked immunoadsorbent assay analysis, and using a series of amino acid deletion mutants, the binding epitope of ATOM-2 was precisely mapped. This showed that ATOM-2 recognizes the C-terminal part of the 15-meric S-tag in context with a few residues of vector encoded sequences. The core sequence for ATOM-2 binding epitope is \"His-Met-Asp-Ser-Pro-Asp-Leu-Gly-Thr,\" which is present in all pET-expression vectors encoding S-tag fusion proteins. Because the ATOM-2 binding region does not overlap with the S-protein binding sequence, a convenient tool is provided for the simultaneous or alternative detection, purification, and analysis of recombinant S-tagged proteins to conventional S-proteins.","PeriodicalId":55044,"journal":{"name":"Hybridoma","volume":"20 1 1","pages":"17-23"},"PeriodicalIF":0.0,"publicationDate":"2001-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/027245701300060364","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60497910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Pharmacokinetics study of a novel chimeric single-chain variable fragment antibody against western equine encephalitis virus. 新型嵌合单链可变片段抗体抗西部马脑炎病毒药代动力学研究。

Hybridoma Pub Date : 2001-02-01 DOI: 10.1089/027245701300060328

M. C. Long, Kelly E.J. Marshall, Brian J. Kearney, George V. Ludwig, Jonathan P. Wong, L. Nagata

{"title":"Pharmacokinetics study of a novel chimeric single-chain variable fragment antibody against western equine encephalitis virus.","authors":"M. C. Long, Kelly E.J. Marshall, Brian J. Kearney, George V. Ludwig, Jonathan P. Wong, L. Nagata","doi":"10.1089/027245701300060328","DOIUrl":"https://doi.org/10.1089/027245701300060328","url":null,"abstract":"A novel recombinant single-chain fragment variable (scFv) antibody against western equine encephalitis (WEE) virus has been previously constructed and partially characterized. The RS10B5huFc antibody was made by fusing an anti-WEE scFv to a human heavy-chain IgG1 constant region. The RS10B5huFc antibody was functional in binding to WEE virus in enzyme-linked immunosorbent assays (ELISAs), and the Fc domain of the antibody was capable of effector functions, such as binding to protein G and human complement. In this study, the RS10B5huFc antibody was further characterized by BIAcore analyses and was found to possess a binding affinity to a WEE virus epitope (K[D] = 9.14 x 10(-6) M), 4.5-fold lower than its parental mouse monoclonal antibody (MAb) 10B5 E7E2 (K[D] = 2 x 10(-6) M). No cross-reactivity was found between the RS10B5huFc antibody and three other alphaviruses (Sindbis virus [SIN], Venezuelan equine encephalitis [VEE] virus, and eastern equine encephalitis [EEE] virus). Pharmacokinetics studies showed that the RS10B5huFc antibody (free and encapsulated) was found to be retained in the lungs of mice for greater than 48 h when administered intranasally. In contrast, when administered intramuscularly to mice, the RS10B5huFc antibody was not detected in the lungs and only found in the liver and kidneys.","PeriodicalId":55044,"journal":{"name":"Hybridoma","volume":"20 1 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2001-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/027245701300060328","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60497935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7