Hybridoma最新文献_第7页

ior egf/r3 anti-human EGF receptor. egf/r3抗人egf受体。

Hybridoma Pub Date : 2001-01-01 DOI: 10.1089/02724570152057689

引用次数: 0

MAbs Against Progesterone Hormone. 抗黄体酮单克隆抗体。

Hybridoma Pub Date : 2001-01-01 DOI: 10.1089/027245701300060625

引用次数: 0

NM-01 Anti-HIV. NM-01抗艾滋病病毒。

Hybridoma Pub Date : 2001-01-01 DOI: 10.1089/027245701300060607

引用次数: 0

HA1, HA2 Anti-Human Arginase. HA1, HA2抗人精氨酸酶。

Hybridoma Pub Date : 2001-01-01 DOI: 10.1089/02724570152057652

引用次数: 0

MAb HT10 (anti-mucin). 单克隆抗体HT10(抗粘蛋白)。

Hybridoma Pub Date : 2001-01-01 DOI: 10.1089/02724570152057698

引用次数: 0

RJS8-1,Mouse Monoclonal to FK-520 (Anti-Ascomycin) RJS8-1小鼠抗子囊霉素(FK-520)单克隆

Hybridoma Pub Date : 2001-01-01 DOI: 10.1089/027245701750293556

David E. Hansen, R. Roberts, Zhongde Wang, R. Plourde, R. J. Sugasawara

引用次数: 0

MAM-6C-8 (Anti-Penicillin Acylase). MAM-6C-8(抗青霉素酰化酶)。

Hybridoma Pub Date : 2001-01-01 DOI: 10.1089/02724570152057643

引用次数: 0

Anti-TNP monoclonal antibodies as reagents for enzyme immunoassay (ELISA). 抗tnp单克隆抗体作为酶免疫测定(ELISA)试剂。

Hybridoma Pub Date : 2000-12-01 DOI: 10.1089/027245700750053968

P. Leo, P. Ucelli, E. Augusto, M. S. Oliveira, W. Tamashiro

{"title":"Anti-TNP monoclonal antibodies as reagents for enzyme immunoassay (ELISA).","authors":"P. Leo, P. Ucelli, E. Augusto, M. S. Oliveira, W. Tamashiro","doi":"10.1089/027245700750053968","DOIUrl":"https://doi.org/10.1089/027245700750053968","url":null,"abstract":"The aim of this study was to produce anti-TNP monoclonal antibodies (MAbs) that could be conjugated and used for the detection of antigen-antibody reactions, in which the antigen specific-antibody had been previously bound to trinitrophenyl (TNP). For hybridoma production, SP2/0-Ag14 cells were fused with spleen cells from mice previously immunized with TNP-ovalbumin (TNP-OVA). After 10 days, enzyme-linked immunoadsorbent assay (ELISA) was used to detect anti-TNP antibodies in the supernatants, and five cultures were found to be strictly positive for TNP. Three of these were subsequently cloned by limiting dilution, and 15 clones were chosen for expansion based on the criterion of high reactivity against TNP. Anti-TNP MAbs produced by those clones were isotyped as IgG1, and purified by Sepharose-protein G affinity cromatography from ascites developed in BALB/c mice. Two purified MAbs (1B2.1B6 and 1B2.1E12) were coupled to horseradish peroxidase (HRPO). The resulting conjugates were evaluated in ELISA tests for interferon-gamma and interleukin-4 detection, in which the secondary anti-cytokine antibodies were coupled either to TNP or biotin. The performance of anti-TNP conjugates in these assays were compared with a biotin-streptavidin/peroxidase system. Both types of conjugates were similarly able to detect cytokines with r2 (linear correlation coefficient) close to unity value. Growth studies of one of those hybridomas (1B2.1B6) yielded a specific growth rate of 0.042 h(-1) and a doubling time of 16.5 h. Data discussed here show that at least two MAbs against TNP raised in this work can be used as a reagent for enzyme immunoassays.","PeriodicalId":55044,"journal":{"name":"Hybridoma","volume":"19 6 1","pages":"473-9"},"PeriodicalIF":0.0,"publicationDate":"2000-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/027245700750053968","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60497201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Characterization of monoclonal antibodies against mouse and rat platelet glycoprotein V (CD42d). 抗小鼠和大鼠血小板糖蛋白V (CD42d)单克隆抗体的鉴定。

Hybridoma Pub Date : 2000-12-01 DOI: 10.1089/027245700750053940

N. Sato, N. Kiyokawa, K. Takada, M. Itagaki, M. Saito, T. Sekino, T. Suzuki, T. Taguchi, K. Mimori, F. Lanza, J. Fujimoto

引用次数: 15

Virolysis and in vitro neutralization of HIV-1 by humanized monoclonal antibody hNM-01. 人源化单克隆抗体hNM-01对HIV-1的病毒裂解和体外中和。

Hybridoma Pub Date : 2000-12-01 DOI: 10.1089/027245700750053913

M. Nakamura, M. Terada, H. Sasaki, M. Kamada, T. Ohno

{"title":"Virolysis and in vitro neutralization of HIV-1 by humanized monoclonal antibody hNM-01.","authors":"M. Nakamura, M. Terada, H. Sasaki, M. Kamada, T. Ohno","doi":"10.1089/027245700750053913","DOIUrl":"https://doi.org/10.1089/027245700750053913","url":null,"abstract":"Antibody humanization by transplanting the complimentarity determining region (CDR) to a human framework aims to reduce the response of the human immune system against a foreign molecule during passive immunization. We transferred the CDR from the murine monoclonal antibody (MAb) NM-01 to a human IgG frame. The humanized NM-01 (hNM-01) recognizes the same epitope on Human Immunodeficiency Virus type 1 (HIV-1) envelope as its murine progenitor, but with greater efficiency, and shows enhanced neutralization of HIV-1. We have shown that this increase in reactivity may be attributed to residue 4 of the humanized kappa chain, where the presence of a methionine residue rather than the murine leucine appears to promote a more advantageous conformation of the antigen-binding site, perhaps via packing interactions with the V(kappa) CDR1. The capacity of humanized NM-01 to neutralize direct clinical isolates was also examined with the expectation that hNM-01 will prove suitable for development as a therapeutic agent. This reshaped antibody reacted with several clinical isolates of HIV-1 tested. Moreover, we proved the ability of this antibody of its activation of complement by flow cytometry and electron microscopy analysis. Although hNM-01 alone was capable of neutralizing HIV-1, the presence of complement enhanced neutralization. The enhancement of complement activation was also observed in hNM-01 than murine progenitor. This finding supports a potential role for antibody-dependent complement-mediated virolysis and more effective neutralization in HIV-1 therapy.","PeriodicalId":55044,"journal":{"name":"Hybridoma","volume":"19 6 1","pages":"427-34"},"PeriodicalIF":0.0,"publicationDate":"2000-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/027245700750053913","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60497105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5