Invertebrate Systematics最新文献

{"title":"Phylogenomics of endemic Australian Ulopinae (Hemiptera: Cicadomorpha: Cicadellidae)","authors":"Olivia Evangelista, Nikolai Tatarnic, Keith Bayless","doi":"10.1071/is23035","DOIUrl":"https://doi.org/10.1071/is23035","url":null,"abstract":"<p>Ulopinae is a distinctive subfamily of leafhoppers that is widely distributed across the Afrotropical, Palearctic, Indomalayan and Australasian regions. The ulopine fauna of Australia is entirely endemic and includes two tribes of striking appearance, the Ulopini and Cephalelini. Knowledge of these groups is fragmentary and in many instances, no information is available beyond original descriptions. We assess the monophyly, phylogenetic placement and species-level diversity of the Ulopini genus <i>Austrolopa</i>. Phylogenetic analyses based on sequence data from target nuclear loci (<i>18S</i>, <i>28S</i>, <i>H2A</i> and <i>H3</i>) and mitochondrial genomes (15 genes) for 23 membracoid taxa yielded congruent topologies. Our results provide strong evidence for the monophyly of Ulopinae and a clade consisting of Ulopini + Cephalelini. However, a non-monophyletic Cephalelini arises from within a polyphyletic Ulopini. <i>Austrolopa</i> was strongly recovered as monophyletic in all analyses, a result also supported by morphological features. The genus currently includes six species, three of which are described based on morphological and molecular data: <i>Austrolopa botanica</i>, <b>sp. nov.</b>, <i>Austrolopa rotunda</i>, <b>sp. nov.</b> and <i>Austrolopa sublima</i>, <b>sp. nov.</b> A lectotype designation is provided for <i>Austrolopa kingensis</i> Evans, 1937, <b>sp. reval.</b> Our findings illustrate that the Australian Ulopinae is far more diverse than currently circumscribed and several species of <i>Austrolopa</i> are yet to be recognised.</p><p>ZooBank: urn:lsid:zoobank.org:pub:1480285B-8F61-4659-A929-2B1EF3168868</p>","PeriodicalId":54927,"journal":{"name":"Invertebrate Systematics","volume":"30 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139765316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sample design in biodiversity studies matters: a fine-scale study of Lawrence’s velvet worm, Peripatopsis lawrencei (Onychophora: Peripatopsidae), reveals hidden diversity","authors":"Julian A. Nieto Lawrence, Savel R. Daniels","doi":"10.1071/is23051","DOIUrl":"https://doi.org/10.1071/is23051","url":null,"abstract":"<p>A fine-scale phylogenetic and phylogeographic analysis of <i>Peripatopsis lawrencei</i> s.l. was conducted with both mitochondrial and nuclear DNA sequence data, using both external morphology and scanning electron microscopy of taxonomically important characters. A total of 119 sequences were used for the mitochondrial cytochrome <i>c</i> oxidase subunit I (<i>COI</i>) whereas a single representative specimen from each locality was sequenced for the nuclear <i>18S</i> rRNA locus. Phylogenetic analyses were conducted on the total <i>COI</i> data set and the combined <i>COI</i> + <i>18S</i> rRNA data set using a Bayesian analysis and maximum likelihood analyses. For the combined DNA sequence data set, a divergence time estimation was further undertaken in BEAST and specimens placed in a phylogenetic framework including all the described <i>Peripatopsis</i> species from South Africa. In addition, a phylogeographic study was conducted exclusively on <i>P. lawrencei</i> s.s. (clade A) using an analysis of molecular variance and haplotype network. Phylogenetic results indicated that, at the Oubos sample locality, two highly distinct genetic lineages were present (clades A and B), whereas a divergence time estimation suggests a Miocene cladogenesis of the novel Oubos lineage. Marked phylogeographic structure was observed for <i>P. lawrencei</i> s.s. (restricted to clade A) across the distribution range with limited maternal dispersal. Morphologically, the two sympatric lineages at Oubos A and B differed in leg pair number, ventral colour and dorsal scale rank counts, as evident from scanning electron microscopy. Our results support the recognition of a distinct species that occurs in sympatry with <i>P. lawrencei</i> s.s. The new species<i>, P. aereus</i> sp. nov. (clade B) is described and the implication for fine-scale taxonomic studies on saproxylic taxa is discussed.</p><p>ZooBank: urn:lsid:zoobank.org:pub:AB6E0BDA-7B5F-4FD3-A863-BA7C814E278C</p>","PeriodicalId":54927,"journal":{"name":"Invertebrate Systematics","volume":"276 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139765124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Castaways: the Leeward Antilles endemic spider genus <i>Papiamenta</i> (Araneae: Pholcidae).","authors":"Bernhard A Huber, Guanliang Meng, Tim M Dederichs, Peter Michalik, Martin Forman, Jirí Král","doi":"10.1071/IS23052","DOIUrl":"10.1071/IS23052","url":null,"abstract":"<p><p>Ninetinae is a group of small to tiny short-legged spiders largely restricted to arid habitats. Among daddy-long-legs spiders (Pholcidae) this is by far the least diverse subfamily but this may partly be a result of inadequate collecting, poor representation in collections or scientific neglect. We build on a large recent collection of the ninetine genus Papiamenta Huber, 2000 from the Leeward Antilles and use cytochrome oxidase 1 (COI ) sequences, extensive scanning electron microscopy data, transmission electron microscopy data and karyotyping to analyse this geographically isolated and poorly known island genus. COI sequences support the split between the two morphologically distinct species on Curaçao but genetic distances between these are surprisingly low (7.4-9.8%; mean 8.6%). The type species P. levii (Gertsch, 1982) may include more than one species but COI and morphology suggest conflicting clade limits. A third species, P. bonay Huber sp. nov. is newly described from Bonaire. Our data on sperm ultrastructure and karyology are puzzling as these suggest different phylogenetic affinities of Papiamenta to other genera. Males transfer sperm as individual sperm (cleistosperm), agreeing with the putative closest relatives as suggested by molecular data, the North American genera Pholcophora and Tolteca . The sex chromosome system (X 1 X 2 X 3 Y ) of P. levii , however, is as in the South American Ninetinae genera Gertschiola and Nerudia but different from the putative closest relatives. ZooBank: urn:lsid:zoobank.org:pub:7A6A2E84-3A61-4637-AF6F-0E31A9FA79A8.</p>","PeriodicalId":54927,"journal":{"name":"Invertebrate Systematics","volume":"38 ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140923825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrative taxonomic approach to the Atlantic Hansarsia (formerly Nematoscelis) yields new krill taxa (Crustacea: Euphausiidae)","authors":"D. N. Kulagin, U. V. Simakova, A. A. Lunina, A. L. Vereshchaka","doi":"10.1071/is23034","DOIUrl":"https://doi.org/10.1071/is23034","url":null,"abstract":"<p>A recent molecular phylogenetic and biogeographic study on the krill genus <i>Hansarsia</i> revealed undescribed cryptic diversity in the Atlantic. Each of four species analysed encompassed robust molecular clades that were linked to dimorphic males in <i>H. microps</i>, <i>H. atlantica</i> and <i>H. tenella</i>. We tested the robustness and divergence of the observed clades using an integrative approach including (1) three independent species delimitation methods for the mitochondrial <i>COI</i> gene (ASAP, GMYC, bPTP), (2) variability of two nuclear genes (<i>H3</i> and <i>ITS1</i>) and (3) morphological analysis (MDS and PCA) with a dataset of 22 characters scored for 131 specimens. Both molecular and morphological analyses resulted in at least six distinct clades within the Atlantic <i>Hansarsia.</i> The strongest divergence was revealed between the two clades of <i>H. tenella</i>, one of which we diagnosed as a new species. Two clades of <i>H. megalops</i> also showed significant divergence but in the absence of males, we were reluctant to designate new species. Different clades linked to male forms in <i>H. microps</i> and <i>H. atlantica</i> are suggested as an incipient species. We also hypothesise an unusual trend in the evolution of euphausiids, in which visual recognition enhances tactile interaction during mating. Our results show that analyses of ostensibly well studied groups may yet yield taxonomic surprises.</p><p>ZooBank: urn:lsid:zoobank.org:pub:AE045636-50EF-450A-B9B3-9231E8B91522</p>","PeriodicalId":54927,"journal":{"name":"Invertebrate Systematics","volume":"31 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139495995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lumping three nominal species into one: taxonomic revision of amphibian parasitic leeches of Torix in Far East Asia (Hirudinea: Glossiphoniidae)","authors":"Chiaki Kambayashi, Takafumi Nakano","doi":"10.1071/is23042","DOIUrl":"https://doi.org/10.1071/is23042","url":null,"abstract":"<p>Although most members of the freshwater leech family Glossiphoniidae have mid-body somites divided into three annuli, the genus <i>Torix</i> Blanchard, 1893 is distinguished by two-annuli somites. <i>Torix</i> has high species richness in Far East Asia, and three nominal species have been recognised in the Japanese Archipelago and adjacent regions that can be distinguished by a combination of both internal and external morphological characteristics. However, recent studies have shown that these diagnostic features are ontogenetically variable and this has resulted in taxonomic confusion among <i>Torix</i> species endemic to the Japanese Archipelago. In this study, we revisit the taxonomic accounts of <i>T. orientalis</i> (Oka, 1925) and <i>T. tagoi</i> (Oka, 1925), in addition to that of the recently redescribed <i>T. tukubana</i> (Oka, 1935) to clarify the diagnostic characteristics for each of the three species. Our morphological and molecular phylogenetic analyses demonstrated that the three <i>Torix</i> species in Japan are indistinguishable. We therefore conclude that these species should be synonymised and treated as a single species. The specific names <i>orientalis</i> and <i>tagoi</i> were simultaneously established under the genus <i>Oligobdella</i> Moore, 1918, therefore we acted as First Reviser and gave precedence to the name <i>O. tagoi</i>, thus the valid name for the Far East Asian <i>Torix</i> species is <i>T. tagoi</i> unless <i>T. orientalis</i> and <i>T. tagoi</i> are treated as distinct species. There are several type localities for <i>T. tagoi</i> and the name-bearing types have been lost, therefore we designate a neotype for this species to obviate zoological and nomenclatural issues.</p><p>ZooBank: urn:lsid:zoobank.org:pub:E2DBF999-3B51-456F-AB04-A7D138E0AF2D</p>","PeriodicalId":54927,"journal":{"name":"Invertebrate Systematics","volume":"111 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138560369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genitalic morphology and phylogenomic placement of the Australian spider Paraplectanoides crassipes Keyserling, 1886 (Araneae, Araneidae) with a discussion on the classification of the family Araneidae","authors":"Gustavo Hormiga, Siddharth Kulkarni, Miquel Arnedo, Dimitar Dimitrov, Gonzalo Giribet, Robert J. Kallal, Nikolaj Scharff","doi":"10.1071/is23050","DOIUrl":"https://doi.org/10.1071/is23050","url":null,"abstract":"<p>We complement and expand the existing descriptions of the Australian araneid spider <i>Paraplectanoides crassipes</i> Keyserling, 1886, and provide the first detailed analysis of the male palpal homologies to include examination of the expanded organ and scanning electron micrographs of the palpal sclerites. We study the placement of <i>Paraplectanoides</i> and the classification of the family Araneidae by combining ultraconserved elements with Sanger markers. We also added Sanger sequences of the Australian araneid genus <i>Venomius</i> to the molecular dataset of Scharff <i>et al</i>. (2020) to explore the phylogenetic placement and implications for classification of the family. We evaluate a recent proposal on the classification of the family Araneidae by Kuntner <i>et al.</i> (2023) in which a new family is erected for <i>P. crassipes</i>. <i>Paraplectanoides</i> is monotypic. Examination of the type material shows that <i>Paraplectanoides kochi</i> O. Pickard-Cambridge, 1877 is misplaced in the genus and the name is a senior synonym of the araneid <i>Isoxya penizoides</i> Simon, 1887 (<b>new synonymy</b>) that results in the <b>new combination</b> <i>Isoxya kochi</i> (O. Pickard-Cambridge, 1877). The classification of Araneidae is revised and the following nomenclatural acts are introduced: Paraplectanoididae Kuntner, Coddington, Agnarsson and Bond, 2023 is a junior synonym of Araneidae Clerck, 1757 <b>new synonymy</b>; phonognathines and nephilines are subfamilies of Araneidae (Subfamily Phonognathinae Simon, 1894 <b>rank resurrected</b>; and Subfamily Nephilinae Simon, 1894 <b>rank resurrected</b>). The results of our analyses corroborate the sister group relationship between <i>Paraplectanoides</i> and the araneid subfamily Nephilinae. <i>Venomius</i> is sister to the Nephilinae + <i>Paraplectanoides</i> clade. The placement of the oarcine araneids and <i>Venomius</i> renders the family Araneidae non-monophyletic if this were to be circumscribed as in Kuntner <i>et al</i>. (2023). In light of the paucity of data that the latter study presents, and in absence of a robust, stable and more densely sampled phylogenetic analysis of Araneidae, the changes and definitions introduced by that classification are premature and could lead to a large number of new families for what once were araneid species if the maximum-crown-clade family definitions were to be used. Consequently, we argue for restoring the familial and subfamilial classification of Araneidae of Dimitrov <i>et al</i>. (2017), Scharff <i>et al</i>. (2020) and Kallal <i>et al</i>. (2020).</p>","PeriodicalId":54927,"journal":{"name":"Invertebrate Systematics","volume":"6 1-2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138513011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New hypothesis of the tribal placement puzzle of Enigmadiplosis (Diptera: Cecidomyiidae), with description of a new pest species on Rhododendron (Ericaceae) in Japan","authors":"Ayman Khamis Elsayed, Yasuko Kobayashi, Raymond J. Gagné, Makoto Tokuda","doi":"10.1071/is23033","DOIUrl":"https://doi.org/10.1071/is23033","url":null,"abstract":"<p>The monotypic genus <i>Enigmadiplosis</i> Harris (Diptera: Cecidomyiidae) was placed in the tribe Clinodiplosini based on adult morphology, but the genus is somewhat puzzling because larvae are typical for the tribe Cecidomyiini. In this study, we describe <i>Enigmadiplosis harrisi</i> sp. nov., a pest species that damages young leaves of the ornamental shrub <i>Rhododendron pulchrum</i> in Mie Prefecture, Honshu, Japan. We also conducted a molecular phylogenetic analysis to confirm the tribal placement. The result clearly indicates that <i>Enigmadiplosis</i> belongs to Cecidomyiini and we therefore assign the genus to that tribe. In addition, we redescribe <i>Clinodiplosis rhododendri</i> (Felt) that forms leaf-roll galls on <i>Rhododendron</i> spp. in eastern North America to allay future questions as to the identity and show that this is unrelated to the new Japanese species.</p><p>ZooBank: urn:lsid:zoobank.org:pub:D2B4A731-271F-4A11-90BB-258725FB9F45</p>","PeriodicalId":54927,"journal":{"name":"Invertebrate Systematics","volume":"20 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138513020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogeny of freshwater mollusc genus Brotia H. Adams, 1866 (Gastropoda: Pachychilidae) from north-east India","authors":"Anushree S. Jadhav, Surya Narayanan, Chinta Sidharthan, F. Köhler, N. A. Aravind","doi":"10.1071/IS23037","DOIUrl":"https://doi.org/10.1071/IS23037","url":null,"abstract":"ABSTRACT The pachychilid genus Brotia H. Adams, 1866, distributed across South and South-East Asia, comprises 46 currently accepted species. Although Brotia has recently been revised, the systematic treatment of the Indian species requires further scrutiny. This study aims to resolve the phylogenetic relationships between Brotia species from north-east India and estimate the divergence using two mitochondrial markers, cytochrome c oxidase subunit I (COI) and 16S ribosomal RNA (16S). Species delimitation analyses have revealed six to nine distinct species in north-east India instead of the single currently known species. We confirm the monophyly of Brotia from the entire distributional range in South and South-East Asia and show that all Indian species form a single clade nested within the South-East Asian radiation. We conclude that Brotia has a South-East Asian origin, with two independent dispersals into India since the early Eocene and through the Oligocene, eventually splitting from ancestors during the late Cretaceous. Further integrative taxonomic research is needed to resolve the taxonomic status of the candidate species delineated herein and reveal the exact number of species in north-east India. This is the first phylogenetic analysis of Brotia from India, significantly improving our understanding of Indian freshwater gastropod fauna.","PeriodicalId":54927,"journal":{"name":"Invertebrate Systematics","volume":"16 6","pages":"772 - 781"},"PeriodicalIF":2.2,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139263321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A preliminary phylogeny for the pseudoscorpion family Garypinidae (Pseudoscorpiones: Garypinoidea), with new taxa and remarks on the Australasian fauna","authors":"Mark S. Harvey","doi":"10.1071/is23029","DOIUrl":"https://doi.org/10.1071/is23029","url":null,"abstract":"The pseudoscorpion family Garypinidae is globally distributed with 79 species in 21 genera and several species represented by Mesozoic and Eocene fossils. This was recently included with the family Larcidae in a unique superfamily, Garypinoidea but there are no phylogenetic hypotheses for the group. Sequence data were obtained for 14 species in 8 genera and numerous outgroup taxa that formed the basis for a preliminary molecular phylogeny. A new subfamily classification is proposed with Protogarypininae, subfamily nov. comprising five genera mostly found in the southern hemisphere, Amblyolpiinae subfamily nov. comprising two genera and Garypininae for the remaining genera. Several new taxa are described including the first Australian species of Aldabrinus, A. rixi sp. nov., a new genus from South-East Asia, Nobilipinus, comprising Nobilipinus nobilis (With, 1906), N. vachoni (Redikorzev, 1938) (that is removed from the synonymy of G. nobilis) and five new species, N. affinis, N. galeatus, N. karenae, N. kohi and N. tricosus, and Solinus pingrup sp. nov. from south-western Australia. Paraldabrinus Beier, 1966 is newly synonymised with Aldabrinus, and Indogarypinus Murthy and Ananthakrishan, 1977 is newly synonymised with Solinus. The holotype of Garypinus mirabilis With, 1907 from Hawaii is redescribed but found to be a tritonymph, rendering the generic identity uncertain. ZooBank: urn:lsid:zoobank.org:pub:E15E4705-0697-4208-9338-A778343996CA","PeriodicalId":54927,"journal":{"name":"Invertebrate Systematics","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134885673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards a systematic revision of the superfamily Cyrenoidea (Bivalvia: Imparidentia): species delimitation, multi-locus phylogeny and mitochondrial phylogenomics","authors":"Ruiwen Wu, Lili Liu, Xiongjun Liu, Yingying Ye, Xiaoping Wu, Zhicai Xie, Zhenyuan Liu, Zhengfei Li","doi":"10.1071/is23015","DOIUrl":"https://doi.org/10.1071/is23015","url":null,"abstract":"Cyrenoidea is a superfamily of bivalves (Bivalvia: Imparidentia) currently comprising three families (Cyrenidae, Cyrenoididae and Glauconomidae). The superfamily is widely distributed in marine, brackish and freshwater environments, with an estimated 60 or more living species. Recent phylogenetic results have confirmed the monophyly of Cyrenoidea and placement in Venerida. Nevertheless, a comprehensive phylogenetic analysis of Cyrenoidea remains elusive and the phylogeny is unresolved due to inadequate sampling in previous studies. Moreover, the taxonomy and delimitation of most species, originally based on shell morphology, have not yet been tested with molecular data. Here, we constructed three molecular datasets by sequencing three markers (<i>COI</i>+<i>16S</i> rRNA+<i>28S</i> rRNA) and complete mitogenomes for <i>Geloina coaxans</i> (Gmelin, 1791) and <i>Glauconome virens</i> (Linnaeus, 1767). <i>COI</i> barcoding clarifies the validity of <i>Geloina coaxans</i> and <i>Geloina erosa</i> that have been subject to controversy regarding synonymy. Additionally, the barcoding supports the existence of multiple cryptic species within the <i>Geloina expansa</i> complex. A multi-locus dataset (<i>COI</i>+<i>16S</i> rRNA+<i>28S</i> rRNA) provides the most comprehensive phylogeny of all eight recognised genera of Cyrenoidea to date. Phylogenetic results indicate that the currently recognised family Cyrenidae is polyphyletic. The type species <i>Geloina coaxans</i>, <i>Cyanocyclas limosa</i> (Maton, 1811) and <i>Polymesoda caroliniana</i> (Bosc, 1801) that have long been classified within the family Cyrenidae based on shell morphology, have a closer relationship with <i>Cyrenoida floridana</i> Dall, 1896 than with other Cyrenidae. Therefore we transfer the genera <i>Geloina</i>, <i>Cyanocyclas</i> and <i>Polymesoda</i> from the family Cyrenidae to the family Cyrenoididae. The mitochondrial phylogenomics further support the family-level relationships in Cyrenoidea obtained from the three-gene analyses, confirming that the newly defined Cyrenoididae is closely related to Glauconomidae as the sister group. We observed a novel gene arrangement in <i>Glauconome virens</i>, the first report on the mitogenome of the family Glauconomidae, by comparing gene arrangements. Three patterns of gene rearrangement identified in Cyrenoidea are shared by the families Glauconomidae, Cyrenoididae and Cyrenidae, suggesting that gene arrangements can be a valuable tool for phylogenetic studies.","PeriodicalId":54927,"journal":{"name":"Invertebrate Systematics","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135980891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}