Iranian Journal of Immunology最新文献_第5页

Harnessing the Power of CAR-NK Cells: A Promising Off-the-Shelf Therapeutic Strategy for CD38-Positive Malignancies. 利用 CAR-NK 细胞的力量：CD38阳性恶性肿瘤的现成治疗策略大有可为

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-12-31 Epub Date: 2023-12-16 DOI: 10.22034/iji.2023.100424.2691

Maryam Asadi, Razie Kiani, Vahid Razban, Seyed Nooreddin Faraji, Amirhossein Ahmadi, Jafar Fallahi, Amin Ramezani, Nasrollah Erfani

{"title":"Harnessing the Power of CAR-NK Cells: A Promising Off-the-Shelf Therapeutic Strategy for CD38-Positive Malignancies.","authors":"Maryam Asadi, Razie Kiani, Vahid Razban, Seyed Nooreddin Faraji, Amirhossein Ahmadi, Jafar Fallahi, Amin Ramezani, Nasrollah Erfani","doi":"10.22034/iji.2023.100424.2691","DOIUrl":"10.22034/iji.2023.100424.2691","url":null,"abstract":"Background: CD38 is highly expressed on multiple myeloma (MM) cells and has been successfully targeted by different target therapy methods. This molecule is a critical prognostic marker in both diffuse large B-cell lymphoma and chronic lymphocytic leukemia.Objective: We have designed and generated an anti-CD38 CAR-NK cell applying NK 92 cell line. The approach has potential application as an off-the-shelf strategy for treatment of CD38 positive malignancies.Methods: A second generation of anti-CD38 CAR-NK cell was designed and generated, and their efficacy against CD38-positive cell lines was assessed in vitro. The PE-Annexin V and 7-AAD methods were used to determine the percentage of apoptotic target cells. Flow cytometry was used to measure IFN-γ, Perforin, and Granzyme-B production following intracellular staining. Using in silico analyses, the binding capacity and interaction interface were evaluated.Results: Using Lentivirus, cells were transduced with anti-CD38 construct and were expanded. The expression of anti-CD38 CAR on the surface of NK 92 cells was approximately 25%. As we expected from in silico analysis, our designed CD38-chimeric antigen receptor was bound appropriately to the CD38 protein. NK 92 cells that transduced with the CD38 chimeric antigen receptor, generated significantly more IFN-γ, perforin, and granzyme than Mock cells, and successfully lysed Daudi and Jurkat malignant cells in a CD38-dependent manner.Conclusion: The in vitro findings indicated that the anti-CD38 CAR-NK cells have the potential to be used as an off-the-shelf therapeutic strategy against CD38-positive malignancies. It is recommended that the present engineered NK cells undergo additional preclinical investigations before they can be considered for subsequent clinical trial studies.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 4","pages":"410-426"},"PeriodicalIF":0.9,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138813475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibody Production after COVID-19 Vaccination in Patients with Inborn Errors of Immunity. 先天性免疫错误患者接种新冠肺炎疫苗后的抗体产生。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-12-31 Epub Date: 2023-10-22 DOI: 10.22034/iji.2023.97759.2525

Maryam Nourizadeh, Elham Feizabadi, Milad Mirmoghtadaei, Ashraf Mohammadi, Mohammad Reza Fazlollahi, Leila Moradi, Zahra Pourpak

{"title":"Antibody Production after COVID-19 Vaccination in Patients with Inborn Errors of Immunity.","authors":"Maryam Nourizadeh, Elham Feizabadi, Milad Mirmoghtadaei, Ashraf Mohammadi, Mohammad Reza Fazlollahi, Leila Moradi, Zahra Pourpak","doi":"10.22034/iji.2023.97759.2525","DOIUrl":"10.22034/iji.2023.97759.2525","url":null,"abstract":"Background: Few studies have evaluated COVID-19 vaccine efficacy in patients with inborn errors of immunity (IEI).Objective: To evaluate the levels of antibody (Ab) production and function after COVID-19 vaccination in IEI patients with phagocytic, complement, and Ab deficiencies and their comparison with healthy controls.Methods: Serum samples were collected from 41 patients and 32 healthy controls at least one month after the second dose of vaccination, while clinical evaluations continued until the end of the third dose. Levels of specific anti-receptor-binding domain (RBD) IgG and anti-RBD neutralizing antibodies were measured using EUROIMMUN and ChemoBind kits, respectively. Conventional SARS-CoV-2 neutralization test (cVNT) was also performed. Cutoff values of ≤20, 20-80, and ≥80 (for cVNT and Chemobined) and 0.8-4.2, 4.2-8.5, and ≥8.5 (for EUROIMMUN) were defined as negative/weak, positive/moderate, and positive/significant, respectively.Results: A considerable distinction was observed between the Ab-deficient patients and the controls for Ab concentration (EUROIMMUN, p<0.01) and neutralization (ChemoBind, p<0.001). However, there was no significant difference compared with the other patient groups. A near-zero cVNT in Ab-deficient patients was found compared to the controls (p<0.01). A significant correlation between the two kits was found using the whole data (R2=0.82, p<0.0001).Conclusion: Despite varying degrees of Ab production, all Ab deficient patients, as well as almost half of those with complement and phagocytic defects, did not effectively neutralize the virus (cVNT). In light of the decreased production and efficiency of the vaccine, a revised immunization plan may be needed in IEI.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 4","pages":"400-409"},"PeriodicalIF":0.9,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49685192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway. SPIB敲低通过降低PD-L1 (CD274)表达和灭活JAK/STAT通路抑制卵巢癌细胞的免疫逃逸

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-09-01 DOI: 10.22034/iji.2023.98236.2559

Wenfei Gu, GuangTao Qi, Li Chen

{"title":"SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway.","authors":"Wenfei Gu, GuangTao Qi, Li Chen","doi":"10.22034/iji.2023.98236.2559","DOIUrl":"https://doi.org/10.22034/iji.2023.98236.2559","url":null,"abstract":"Background: Spi-B transcription factor (SPIB) is an E-twenty-six (ETS) transcription factor associated with tumor immunity.Objective: To investigate the functions and mechanisms of SPIB in ovarian cancer (OC) cells.Methods: Cell proliferation, apoptosis, migration, and invasion were determined using colony formation, EdU, flow cytometry, and transwell assays, respectively. The binding sites of programmed death-ligand 1 (PD-L1) and SPIB were predicted using the JASPAR database and verified using the ChIP and luciferase reporter assays.Results: SPIB knockdown inhibited OC cell proliferation, migration, and invasion, and significantly boosted apoptosis (p<0.05). SPIB directly enhanced PD-L1 transcription in OVCAR-3 and SKOV3 cells (p<0.05). Importantly, the JAK/STAT pathway was markedly inactivated in OC cells upon SPIB knockdown. SPIB knockdown markedly decreased JAK2 and STAT1 phosphorylation in OVCAR-3 and SKOV3 cells (p<0.05).Conclusion: These data indicate that SPIB knockdown inhibits OC cell progression by downregulating PD-L1 and inactivating the JAK/STAT pathway.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 3","pages":"335-347"},"PeriodicalIF":0.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10530729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Blocking NLRP3 Inflammasome on Type II Innate Lymphoid Cell Response in Allergic Rhinitis. 阻断 NLRP3 炎症小体对过敏性鼻炎中 II 型先天性淋巴细胞反应的影响

IF 1.1 4区医学

Iranian Journal of Immunology Pub Date : 2023-09-01 DOI: 10.22034/iji.2023.96966.2473

Guoqing Gong, Peng Huang, Changliang Yang, Chengcheng Huang, Zhao Zhang, Ruiyao Chen, Tingfeng Sun, Guang Yang

{"title":"Effects of Blocking NLRP3 Inflammasome on Type II Innate Lymphoid Cell Response in Allergic Rhinitis.","authors":"Guoqing Gong, Peng Huang, Changliang Yang, Chengcheng Huang, Zhao Zhang, Ruiyao Chen, Tingfeng Sun, Guang Yang","doi":"10.22034/iji.2023.96966.2473","DOIUrl":"10.22034/iji.2023.96966.2473","url":null,"abstract":"Background: Type 2 innate lymphoid cells (ILC2s) and NLRP3 inflammasome are related to allergic and inflammatory responses. NLRP3 inflammasome inhibitor MCC950 was demonstrated to ameliorate allergic rhinitis (AR) in animal models.Objective: To elucidate the effect of MCC950 on ILC2 responses in AR.Methods: NLRP3 inflammasome, ILC2s, IL-5+ILC2s, IL-13+ILC2s, and Th2-related factors were examined in 30 AR patients. ILC2s were identified as Lin-CRTH2+CD127+lymphocytes. ILC2s isolated from PBMCs were stimulated with LPS plus ATP. The effect of MCC950, IL-1β, and IL-18 on ILC2 responses was detected by flow cytometry. AR models were established in 60 BALB/c mice. Nasal symptoms and ILC2 responses in the AR models after MCC950 treatment were detected. Human nasal epithelial cells were stimulated with IL-13 (10 ng/mL) and treated with MCC950 (10 μM).Results: AR patients showed activated NLRP3 inflammasome and increased ILC2 responses compared to controls. NLRP3 inflammasome levels in the AR patients were positively related to the proportion of ILC2s, IL-5+ILC2s, and IL-13+ILC2s in total PBMCs. MCC950 treatment or IL-1β/IL-18 suppression inhibited ILC2 proliferation and Th2-related factors (GATA3, RORα, IL-5, and IL-13). MCC950 administration alleviated frequencies of nasal rubbing and sneezes in the AR models. ILC2s, IL-5+ILC2s, and IL-13+ILC2s in mice were reduced by MCC950. MCC950 inhibited NLRP3 inflammasome in the in vitro models of AR.Conclusion: MCC950 inhibited ILC2 responses in AR and mice models, suggesting that blocking NLRP3 inflammasome may be a promising target for AR clinical treatment.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"3 20","pages":"287-302"},"PeriodicalIF":1.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10134609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of SARS-CoV-2 Specific Antibodies in Recovered Patients by Different ELISA Kits. 不同ELISA试剂盒对康复患者SARS-CoV-2特异性抗体的评价

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-09-01 DOI: 10.22034/iji.2023.93206.2202

Alireza Fereidouni, Hamidreza Safari, Hadis Rezapoor, Sara Mahmoudzadeh, Mohammad Fereidouni

{"title":"Evaluation of SARS-CoV-2 Specific Antibodies in Recovered Patients by Different ELISA Kits.","authors":"Alireza Fereidouni, Hamidreza Safari, Hadis Rezapoor, Sara Mahmoudzadeh, Mohammad Fereidouni","doi":"10.22034/iji.2023.93206.2202","DOIUrl":"https://doi.org/10.22034/iji.2023.93206.2202","url":null,"abstract":"Background: The coronavirus disease 2019 (COVID-19) was first reported in December 2019 in Wuhan, Hubei Province of China. As long as the 27th of December 2021, approximately 280 million people have been infected with coronavirus, resulting in more than 5,418,421 deaths worldwide. Since the beginning of the COVID-19 pandemic, different methods were introduced for diagnosing coronavirus-infected patients and evaluating the immune response, following the vaccination.Objective: The current study aimed to compare the level of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) specific IgG in a group of patients who recovered from COVID-19, measured by three different enzyme-linked immunosorbent assay (ELISA) kits.Methods: This cross-sectional study was conducted on sera from patients who recovered from a real-time reverse transcriptase-polymerase chain reaction (RT-PCR)-confirmed COVID-19 in Birjand, South Khorasan, Iran. SARS-CoV-2 anti-nucleocapsid (N) and spike (S) protein IgG levels were measured using commercial ELISA kits. Comparison between groups was made using one-way ANOVA and Tukey post hoc tests.Results: The mean titer of anti-N IgG was significantly higher for the PishtazTeb Diagnostics kit than the Ideal Tashkhis Atieh kit (p<0.05). There was no correlation between the titer of anti-N IgG (PishtazTeb Diagnostics and Ideal Tashkhis Atieh) and anti-S IgG (Chemobind Company) antibodies.Conclusion: This study indicates that the domestic ELISA kits have variable but acceptable sensitivity for detecting SARS-CoV-2 specific IgG antibodies.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 3","pages":"374-381"},"PeriodicalIF":0.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10198886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Implications of Complement Imbalance in COVID-19: A Molecular Mechanistic Discussion on the Importance of Complement Balance. COVID-19 中补体失衡的影响：关于补体平衡重要性的分子机制讨论

IF 1.1 4区医学

Iranian Journal of Immunology Pub Date : 2023-09-01 DOI: 10.22034/iji.2023.97585.2522

Mehdi Torabizadeh, Helia Modaresi Asfeh, Zeinab Deris Zayeri, Cerdan Dominique, Hashem Kazemi, Najmaldin Saki

{"title":"Implications of Complement Imbalance in COVID-19: A Molecular Mechanistic Discussion on the Importance of Complement Balance.","authors":"Mehdi Torabizadeh, Helia Modaresi Asfeh, Zeinab Deris Zayeri, Cerdan Dominique, Hashem Kazemi, Najmaldin Saki","doi":"10.22034/iji.2023.97585.2522","DOIUrl":"10.22034/iji.2023.97585.2522","url":null,"abstract":"Two central questions in COVID-19 treatment which should be considered are: \"How does the imbalance of the complement system affect the therapeutic approaches?\" and \"Do we consider complement inhibitors in therapeutic protocols?\". The complement system is a double-edged sword since it may either promote immune responses against COVID-19 or contribute to destructive inflammation in the host. Therefore, it is crucial to regulate this system with complement inhibitors. In this manuscript, we discuss the molecular mechanisms of complement and complement inhibitors in COVID-19 patients. We searched the terms \"COVID-19\", \"Complement\", \"Complement inhibitor\", \"SARS-CoV-2\", and all complement fragments and inhibitors from 2000 to 2022 in PubMed and google scholar and checked the pathways in \"KEGG pathway database\". Complement is not well-appreciated in the treatment protocols despite its multiple roles in the disease, and most of the preventive anti-inflammatory therapeutic approaches did not include a complement inhibitor in COVID-19 therapeutic protocols. In this review article, we discussed the most recent studies regarding complement components mediated interventions and the mechanism of these interventions in COVID-19 patients. Since the control of the complement system overactivation is associated with a better prognosis in the initial stages of COVID-19, heparin, anti-thrombin, C1-inhibitor, montelukast, and hydralazine can be effective in the initial stages of this viral infection. Recombinant complement activation (RCA) proteins are more effective in regulating complement compared to terminal pathway therapeutic approaches such as the C3a and C5a inhibitors.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"3 20","pages":"247-261"},"PeriodicalIF":1.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10516300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased Serum Levels of IL-1β after Ischemic Stroke are Inversely Associated with Vitamin D. 缺血性卒中后血清IL-1β水平升高与维生素D呈负相关。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-09-01 DOI: 10.22034/iji.2023.96384.2432

Mahnaz Bayat, Niloufar Razavi Moosavi, Najmeh Karimi, Moosa Rahimi, Afshin Borhani-Haghighi

{"title":"Increased Serum Levels of IL-1β after Ischemic Stroke are Inversely Associated with Vitamin D.","authors":"Mahnaz Bayat, Niloufar Razavi Moosavi, Najmeh Karimi, Moosa Rahimi, Afshin Borhani-Haghighi","doi":"10.22034/iji.2023.96384.2432","DOIUrl":"https://doi.org/10.22034/iji.2023.96384.2432","url":null,"abstract":"Background: The initial inflammatory reaction starts following occlusion in ischemic stroke (IS). Interleukin-1β (IL-1β) is a pro-inflammatory cytokine with a crucial role in the pathogenesis of neurodegenerative disorders.Objective: To investigate the levels of IL-1β and vitamin D (VitD) in patients with IS compared with controls and their correlation.Methods: The serum level of 25-OH VitD and IL-1β was assessed in 102 IS patients (0-24 h after stroke) and 102 controls with an enzyme-linked immunosorbent assay (ELISA) kit.Results: We found a significant increase in IL-1β (80.14±6.8 vs. 60.32±4.1 pg/ml, p<0.05) and a decrease in VitD level (24.3±1.4 vs. 29.9±1.5 ng/ml, p<0.01) in the IS patients compared with the controls. There was a significantly positive correlation between the National Institutes of Health Stroke Scale (NIHSS) and IL-1β according to both the Spearman correlation (r=0.35, p=0.0003) and the linear regression (beta=0.255, p=0.014). Also, a significant negative association between VitD and NIHSS was detected by the Spearman correlation (r=-0.41, p<0.0001) and the linear regression (beta=-0.381, p=0.000). Moreover, we found a significant negative correlation (r=-0.26, p=0.006) between the serum levels of VitD and IL-1β in the patient group.Conclusion: Ischemic stroke correlates positively with IL-1β and negatively with VitD levels. The speculated role of VitD deficiency in the evolution and severity of stroke may be justified by its role in the modification of inflammation.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 3","pages":"359-367"},"PeriodicalIF":0.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10167948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elevated HSP70 and HSP90 as Predictive Markers of Immune Activation and Lung Injury in SARS-COV-2 Disease. HSP70和HSP90升高作为SARS-COV-2疾病中免疫激活和肺损伤的预测标志物

IF 1.1 4区医学

Iranian Journal of Immunology Pub Date : 2023-09-01 DOI: 10.22034/iji.2023.97202.2488

Zivar Zangeneh, Gholamreza Khamisipour

{"title":"Elevated HSP70 and HSP90 as Predictive Markers of Immune Activation and Lung Injury in SARS-COV-2 Disease.","authors":"Zivar Zangeneh, Gholamreza Khamisipour","doi":"10.22034/iji.2023.97202.2488","DOIUrl":"10.22034/iji.2023.97202.2488","url":null,"abstract":"Background: Heat shock proteins (HSPs) are involved in innate and adaptive immune responses, especially inflammatory responses due to immune cell activation. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was one of the most important causes of death in the recent pandemic. Increased cellular stress and excessive inflammation are common in coronavirus disease-19 (COVID-19), although the underlying mechanisms are still poorly understood.Objective: To evaluate the relationship between HSP and the pathological effects of COVID-19.Methods: A group of 107 patients was categorized to two populations (mild and severe) based on their chest high-resolution computed tomography (HRCT) results. The HSP70, HSP90 alpha, and serum levels of C-reactive protein (CRP) were measured by enzyme-linked immunosorbent assay (ELISA). Lactate dehydrogenase (LDH), and creatine phosphokinase (CPK) were measured by the automated analyzer.Results: Our data showed increased levels of HSP70 and HSP90 in patients with COVID-19. The HSPs levels were elevated in the severe group compared to the mild group. This study demonstrated a positive correlation between both elevated levels of HSP70, HSP90, and HRCT grade and also a positive correlation with CRP and CPK in the severe group.Conclusion: HSP90 and HSP70 contribute to excessive immune responses and cytokine storms. They may serve as prognostic serum markers for COVID-19 lung injury. Additionally, they are candidates for anti-inflammatory therapy.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"3 20","pages":"368-373"},"PeriodicalIF":1.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10200004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Senescent CD153+ T Lymphocytes Increase in the Peripheral Blood of Patients with Thromboangiitis Obliterans. 闭塞性血栓性脉管炎患者外周血衰老性CD153+ T淋巴细胞升高。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-09-01 DOI: 10.22034/iji.2023.99188.2622

Niloofar Mashhadi, Leila Kasraian, Hamed Ghoddusi Johari, Ahmad Hosseinzadeh, Nasim Kheshtchin, Mehrnoosh Doroudchi

{"title":"Senescent CD153+ T Lymphocytes Increase in the Peripheral Blood of Patients with Thromboangiitis Obliterans.","authors":"Niloofar Mashhadi, Leila Kasraian, Hamed Ghoddusi Johari, Ahmad Hosseinzadeh, Nasim Kheshtchin, Mehrnoosh Doroudchi","doi":"10.22034/iji.2023.99188.2622","DOIUrl":"https://doi.org/10.22034/iji.2023.99188.2622","url":null,"abstract":"Background: Buerger's disease, also known as Thromboangiitis Obliterans (TAO), is a progressive, inflammatory vascular disease with unknown etiology.Objective: To address the degree of T cell immunosenescence in this inflammatory disease, the frequency of senescent T cells expressing CD57 and/or CD153 (CD30L) in patients with TAO.Methods: In this study, nine male cigarette smoker patients with TAO, nine male healthy cigarette smokers, and nine male healthy non-smoker blood donors were enrolled. PBMCs were extracted from the blood of all participants and stored in liquid nitrogen before use. The percentages of senescent T cells were detected by flow cytometry. The results were analyzed using non-parametric statistical tests.Results: The frequencies of senescent CD3+CD4+CD57+CD153+ and CD3+CD4+CD57-CD153+ T cells significantly increased in patients compared with the non-smoker controls (p=0.01 and p=0.04, respectively). The frequency of senescent CD3+CD4-CD57-CD153+ T cells was higher in patients compared with the smoker controls (p=0.02). In patients with TAO, CD57+CD153- cells were more frequent in CD3hiCD4- and CD3hiCD4+ T cells compared with the CD3loCD4- and CD3loCD4+ T cells (p=0.008 and p=0.0002, respectively). Conversely, the frequency of CD57-CD153+ T cells was significantly higher in CD3loCD4- T cells compared with the CD3hiCD4- T cells (p=0.004). The percentage of CD3+CD4+CD57+CD153- T cells correlated negatively with smoking level in smoker controls (p=0.02, Spearman r=-0.80).Conclusion: Elevated frequencies of senescent CD4+CD57+CD153+ and CD4+CD57-CD153+ T cells in patients compared with non-smoker and smoker controls suggest the contribution of immunosenescence in TAO.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 3","pages":"262-275"},"PeriodicalIF":0.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10143005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neutrophil Extracellular Traps Formation and Citrullinated Histones 3 Levels in Patients with Kawasaki Disease. 川崎病患者的中性粒细胞胞外陷阱形成和瓜氨酸化组蛋白 3 水平

IF 1.1 4区医学

Iranian Journal of Immunology Pub Date : 2023-09-01 DOI: 10.22034/iji.2023.97562.2523

Jing Hu, Wei Qian, Tianhe Wang, Jingjing Ling, Yingzuo Shi

{"title":"Neutrophil Extracellular Traps Formation and Citrullinated Histones 3 Levels in Patients with Kawasaki Disease.","authors":"Jing Hu, Wei Qian, Tianhe Wang, Jingjing Ling, Yingzuo Shi","doi":"10.22034/iji.2023.97562.2523","DOIUrl":"10.22034/iji.2023.97562.2523","url":null,"abstract":"Background: Kawasaki disease (KD) is a vasculitis associated with vascular injury and autoimmune response. Inflammatory factors stimulate neutrophils to produce web-like structures called neutrophil extracellular traps (NETs). Citrullinated histone 3 (H3Cit) is one of the main protein components of neutrophil extracellular traps involved in the process of NETosis. The levels of NETs and H3Cit in the KD are not known.Objective: To determine the changes in the levels of NETs and H3Cit in KD.Methods: Children with KD were recruited and divided into the acute KD and the sub-acute KD group according to the disease phase and whether intravenous immunoglobulin (IVIG) was used or not. Peripheral venous blood was taken before and after the IVIG administration and sent for the examination of NETs by flow cytometry. The level of H3Cit was measured by enzyme-linked immunosorbent assay (ELISA).Results: The counts of NETs in the acute KD group significantly increased compared with the healthy controls (p<0.01). The level of H3Cit was significantly higher in the acute KD group than in the healthy control subjects. Of note, both the counts of NETs and the level of H3Cit decreased in the KD patients treated with IVIG compared with the acute KD group (p<0.01).Conclusion: Acute KD is characterized by an increased formation of NETs and high levels of H3Cit. IVIG significantly inhibited NETs formation and also reduced the level of plasma H3Cit in children with KD.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"3 20","pages":"327-334"},"PeriodicalIF":1.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10144667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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