评估强直性脊柱炎患者的 PD-1 基因表达谱和调控区甲基化情况

IF 1.1 4区 医学 Q4 IMMUNOLOGY
Iranian Journal of Immunology Pub Date : 2024-06-30 Epub Date: 2024-06-24 DOI:10.22034/iji.2024.101565.2757
Narjes Soleimanifar, Sara Assadiasl, Mohammed Sameer Al-Shammari, Abdolrahman Rostamian, Maryam Sadr, Sepideh Shahkarami, Hanieh Mojtahedi, Mohammad Hossein Nicknam
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引用次数: 0

摘要

背景:强直性脊柱炎(AS)是一种以椎骨关节融合和轴关节炎为特征的慢性自身免疫性疾病。程序性死亡-1(PD-1)抑制受体在控制T细胞功能方面起着关键作用,可能对强直性脊柱炎等自身免疫性疾病的发病机制有重要影响:通过检测PD-1基因调控位点的甲基化CpG岛,研究PD-1基因的表达及其表观遗传调控。方法:采用亚硫酸氢盐转换和甲基化特异性 PCR 技术检测 30 名 AS 患者和 30 名健康人内含子 1 中的 16 个 CpG 岛。此外,还从新鲜外周血单核细胞(PBMCs)中分离出 RNA 样本,合成互补 DNA(cDNA)后,利用实时 PCR 技术评估 PD-1 基因的表达水平:结果:PD-1基因内含子区的CpG岛在强直性脊柱炎患者和健康对照组中均存在高甲基化。与对照组相比,强直性脊柱炎患者的PD-1基因表达明显下调(P=0.017)。巴斯强直性脊柱炎疾病活动指数与PD-1基因表达之间也呈负相关:结论:PD-1基因的低水平表达与强直性脊柱炎的发病机制有关。结论:PD-1基因的低水平表达与强直性脊柱炎的发病机制有关。然而,在两组患者中,PD-1基因内含子1 CpG岛的甲基化水平表明,与内含子的甲基化相比,其他调控机制与PD-1基因的表达更为相关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of PD-1 Gene Expression Profile and Methylation of the Regulatory Regions in Patients with Ankylosing Spondylitis.

Background: Ankylosing spondylitis (AS) is a chronic autoimmune disorder characterized by the fusion of vertebral joints and axial arthritis. The programmed death-1 (PD-1) inhibitory receptor has a pivotal role in controlling T cell function and may have a significant impact on the pathogenesis of autoimmune diseases such as AS pathogenesis.

Objective: To investigate PD-1 gene expression and its epigenetic regulation by detecting methylated CpG islands in the regulatory sites of the gene. This will provide insight into the mechanisms involved in the disease.

Methods: 30 AS patients and 30 healthy individuals were examined to detect the 16 CpG islands in intron 1 using bisulfite conversion and methylation-specific PCR technique. In addition, RNA samples were isolated from fresh peripheral blood mononuclear cells (PBMCs), and after complementary DNA (cDNA) synthesis, the expression level of the PD-1 gene was evaluated using Real-Time PCR.

Results: The CpG islands located in the intronic zone of the PD-1 gene were hyper-methylated in both the patients with AS and the healthy controls. The gene expression of PD-1 was significantly downregulated in AS patients compared with the controls (p=0.017). A negative correlation between the Bath Ankylosing Spondylitis Disease Activity Index and PD-1 gene expression was also revealed.

Conclusion: The low level of PD-1 gene expression is implicated in the pathogenesis of AS. However, in both groups, the methylation level of the intron 1 CpG islands of the PD-1 gene suggests that other regulatory mechanisms are more relevant to PD-1 gene expression than methylation in the intron.

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来源期刊
Iranian Journal of Immunology
Iranian Journal of Immunology Medicine-Immunology and Allergy
CiteScore
1.60
自引率
0.00%
发文量
50
审稿时长
12 weeks
期刊介绍: The Iranian Journal of Immunology (I.J.I) is an internationally disseminated peer-reviewed publication and publishes a broad range of experimental and theoretical studies concerned with all aspects of immunology.
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