European Biophysics Journal最新文献_第3页

Homologous series of N-acylmelatonins: synthesis, biophysical studies, enhanced antioxidant, antimicrobial and anticancer activities n -酰基褪黑素同源系列：合成、生物物理研究、增强抗氧化、抗菌和抗癌活性。

IF 2.3 4区生物学

European Biophysics Journal Pub Date : 2026-05-28 DOI: 10.1007/s00249-026-01849-0

Surendra Pothuraju, Arya Jyothibabu Sajila, Ravi Kanth Kamlekar

{"title":"Homologous series of N-acylmelatonins: synthesis, biophysical studies, enhanced antioxidant, antimicrobial and anticancer activities","authors":"Surendra Pothuraju, Arya Jyothibabu Sajila, Ravi Kanth Kamlekar","doi":"10.1007/s00249-026-01849-0","DOIUrl":"10.1007/s00249-026-01849-0","url":null,"abstract":"<div><p><i>N</i>-acylmelatonins (NAMs), a class of fatty acid amides featuring melatonin as the polar head group, demonstrate significant biomedical potential. In this study, a homologous series of saturated NAMs (<i>n</i> = 9–18) were synthesized and fully characterized <i>via</i> FTIR, NMR and HRMS. The biophysical self-assembly behaviour was assessed by powder X-ray diffraction and fluorescence spectroscopy. The <i>d</i>-spacing determined from PXRD increases linearly with an increment of ~ 0.90 Å per CH₂ group, suggesting that the NAMs adopt a tilted bilayer structure. Fluorescence spectroscopy was employed to measure the critical micellar concentrations (CMCs) of NAMs by monitoring the spectral changes of 8-anilinonaphthalene-1-sulfonate (ANS). Fluorescence emission and lifetime measurements of NAMs show a red-shift (~ 10 nm) in emission maxima and a 2.4-fold emission intensity enhancement due to the hydrophobic acyl chain. Antioxidant properties, determined by DPPH radical scavenging assays, increased with concentration, showcasing potent activity. Notably, NAMs exhibited enhanced antimicrobial efficacy against clinically relevant bacterial and fungal strains, with minimum inhibitory concentrations comparable to standard drugs. <i>In vitro</i> anticancer screening revealed significant cytotoxicity against multiple human cancer cell lines, particularly with medium-chain NAMs. These findings highlight the broad-spectrum therapeutic potential of NAMs, driven by variable acyl chain length, self-assembly characteristics and strong biological applications.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":548,"journal":{"name":"European Biophysics Journal","volume":"55 4","pages":"711 - 729"},"PeriodicalIF":2.3,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148040704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and biophysical characterization of bioengineered cytochrome nanowires 生物工程细胞色素纳米线的合成及生物物理特性研究。

IF 2.3 4区生物学

European Biophysics Journal Pub Date : 2026-05-23 DOI: 10.1007/s00249-026-01848-1

M. Raquel Pacheco, Ana J. Carvalho, Marta A. Silva, Tomás Calmeiro, Nykola C. Jones, Søren V. Hoffmann, Leonor Morgado, M. Manuela A. Pereira, Elvira Fortunato, Carlos A. Salgueiro, Pedro Tavares, Alice S. Pereira

{"title":"Synthesis and biophysical characterization of bioengineered cytochrome nanowires","authors":"M. Raquel Pacheco, Ana J. Carvalho, Marta A. Silva, Tomás Calmeiro, Nykola C. Jones, Søren V. Hoffmann, Leonor Morgado, M. Manuela A. Pereira, Elvira Fortunato, Carlos A. Salgueiro, Pedro Tavares, Alice S. Pereira","doi":"10.1007/s00249-026-01848-1","DOIUrl":"10.1007/s00249-026-01848-1","url":null,"abstract":"<div><p><i>Geobacter</i> bacteria produce multiheme <i>c</i>-type cytochrome nanowires that are involved in long-range extracellular electron transfer. The ability of these protein nanowires to conduct electrical current makes them promising candidates for electronic devices, offering several functional and sustainable advantages over traditional materials. Therefore, this study focused on synthesizing hybrid protein fibers that mimic the natural <i>Geobacter</i> extracellular nanowires. To achieve this, a mutated PpcA triheme protein variant was used as the building block, with thiol-ene coupling employed to bind the protein molecules. This engineered PpcA variant (PpcAK9CK22C) maintained a structure similar to that of the native protein. Thermal denaturation studies revealed a two-state process, with a melting temperature of 62 ± 1 °C and an enthalpy change of 61 ± 2 kcal/mol. The new protein nanowires showed a lower heme group content than the precursor protein and displayed distinct secondary structure features, with a slight reduction in helical content and an increase in β-sheet and unordered structures. Their thermal stability also differed, as it could not be described by the same model applied to the PpcA variant. Despite these differences, the nanowires retained their ability to undergo redox cycling. Morphologically, they consisted of linear single-protein filaments extending over 300 nm in length.</p></div>","PeriodicalId":548,"journal":{"name":"European Biophysics Journal","volume":"55 :","pages":"459 - 469"},"PeriodicalIF":2.3,"publicationDate":"2026-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00249-026-01848-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148004279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tracking the rotational dynamics of Chlamydomonas reinhardtii across culture days using optical tweezers 利用光学镊子跟踪莱茵衣藻在整个培养日的旋转动态。

IF 2.3 4区生物学

European Biophysics Journal Pub Date : 2026-05-11 DOI: 10.1007/s00249-026-01846-3

Shurui Deng, Yanshan Lin, Domna G. Kotsifaki

引用次数: 0

Interaction of Pf4 tactoids with bacteria and synthetic colloidal rods. Pf4亲和体与细菌及合成胶棒的相互作用。

IF 2.3 4区生物学

European Biophysics Journal Pub Date : 2026-05-11 DOI: 10.1007/s00249-026-01844-5

Mariana de Oliveira Silva, Iago Grobas, Abul K Tarafder, Tanmay A M Bharat, Dirk G A L Aarts

{"title":"Interaction of Pf4 tactoids with bacteria and synthetic colloidal rods.","authors":"Mariana de Oliveira Silva, Iago Grobas, Abul K Tarafder, Tanmay A M Bharat, Dirk G A L Aarts","doi":"10.1007/s00249-026-01844-5","DOIUrl":"10.1007/s00249-026-01844-5","url":null,"abstract":"<p><p>We investigated the interaction of liquid crystal droplets, formed by a phase separating system of elongated virus particles and polymer with rod-like impurities. The virus particles were Pf4, which when mixed with sodium alginate as polymer, phase separated into an isotropic phase in coexistence with a nematic phase of the virus particles. The nematic phase appeared in the form of spindle-shaped liquid crystal droplets, which are called tactoids. We studied the interaction of this mixture with micrometer sized rods, which were either bacteria (Bacillus subtilis or Escherichia coli) or inert colloidal particles (made of SU8 photoresist polymer or silica). Confocal laser scanning microscopy was used to examine the mixtures and four different dominant configurations were found, classified as no attachment, partial attachment of the rod to the tactoid, a \"sandwich\" state, where two tactoids interact with the same rod, and lastly encapsulation of the rods by the tactoids. We categorized the results in terms of surface properties and rod geometry (size and aspect ratio). We further investigated the system through numerical calculation in a simplified two-dimensional model. Finally, we addressed the role of confining the experimental system from three dimensions to a quasi two-dimensional setup, a relevant scenario in bacteria biofilms.</p>","PeriodicalId":548,"journal":{"name":"European Biophysics Journal","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7619265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147866321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamics of viral self-assembly, viral genome packaging, and virus-cell interactions studied by optical tweezers 用光学镊子研究病毒自组装、病毒基因组包装和病毒与细胞相互作用的动力学。

IF 2.3 4区生物学

European Biophysics Journal Pub Date : 2026-04-30 DOI: 10.1007/s00249-026-01843-6

Yanping Gong, Dorus T. Harmsen, Wouter H. Roos

{"title":"Dynamics of viral self-assembly, viral genome packaging, and virus-cell interactions studied by optical tweezers","authors":"Yanping Gong, Dorus T. Harmsen, Wouter H. Roos","doi":"10.1007/s00249-026-01843-6","DOIUrl":"10.1007/s00249-026-01843-6","url":null,"abstract":"<div><p>Viruses are complex supramolecular assemblies that propagate their genetic material from cell to cell, thereby relying on host cell mechanisms. Employing a combination of passive and active strategies, they efficiently package, transport and release nucleic acids. While structural and biochemical techniques offer insights into certain, static aspects of the viral life cycle, recent advancements in biophysical approaches now allow for direct measurement of their inherent dynamic activities in the research field commonly referred to as physical virology. One of these methods is optical tweezers, enabling the precise measurement of force and position at the single-molecule level over time. Over the past decades, the ability to optically trap beads and to manipulate biomolecules has revolutionised medical and biophysical research. In this paper, we provide a comprehensive analysis of optical tweezers, exploring its integration with imaging modalities and review its diverse applications in the study of viruses and viral components. In particular we focus on studies that use optical tweezers to study virus-cell interactions, genome packaging using molecular motors and co-assembly of viral assembly proteins with their nucleic acid.</p></div>","PeriodicalId":548,"journal":{"name":"European Biophysics Journal","volume":"55 :","pages":"391 - 411"},"PeriodicalIF":2.3,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00249-026-01843-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Process-analytical study of IL-6 refolding during batch buffer exchange 间歇缓冲液交换过程中IL-6再折叠的过程分析研究。

IF 2.3 4区生物学

European Biophysics Journal Pub Date : 2026-04-22 DOI: 10.1007/s00249-026-01845-4

Yuichi Nakahara, Yuta Endo, Yutaka Matsuda, Shunsuke Yamazaki, Mayumi Watanabe, Yutaka Sato, Masayo Date, Naoyuki Yamada, Yoshiyuki Takahara, Kazutoshi Takahashi, Keisuke Kato, Takahiro Okasora, Daisuke Ejima

{"title":"Process-analytical study of IL-6 refolding during batch buffer exchange","authors":"Yuichi Nakahara, Yuta Endo, Yutaka Matsuda, Shunsuke Yamazaki, Mayumi Watanabe, Yutaka Sato, Masayo Date, Naoyuki Yamada, Yoshiyuki Takahara, Kazutoshi Takahashi, Keisuke Kato, Takahiro Okasora, Daisuke Ejima","doi":"10.1007/s00249-026-01845-4","DOIUrl":"10.1007/s00249-026-01845-4","url":null,"abstract":"<div>\u0000 \u0000 <p>Interleukin-6 (IL-6) is an aggregation-prone cytokine whose recombinant production from Escherichia coli inclusion bodies remains challenging because of inefficient refolding. Here, we present a limited batch-mode process-analytical study comparing two practical buffer-exchange operations, “fast” and “slow”, during batch dilution refolding of IL-6. Using SDS-PAGE, size-exclusion chromatography (SEC), dynamic light scattering (DLS), and far-UV circular dichroism (CD) spectroscopy, we examined dimerization and aggregation under acidic and guanidine denaturation workflows. The data suggest that IL-6 forms predominantly non-covalent dimers and that batch buffer-exchange operation and timescale are associated with aggregation outcome. Because the acidic and guanidine workflows differed in starting solvent composition, residual denaturant, and final protein concentration, the results should be interpreted as comparisons of complete workflow conditions rather than isolated denaturant effects. Overall, the combined use of SEC, DLS, and far-UV CD provides a practical framework for monitoring IL-6 refolding outcomes, while mechanistic assignments remain beyond the scope of this study.</p>\u0000 </div>","PeriodicalId":548,"journal":{"name":"European Biophysics Journal","volume":"55 4","pages":"689 - 695"},"PeriodicalIF":2.3,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Statistical mechanics of soft collagenous tissues: a constitutive model with damage for cerebral bridging veins 软胶原组织的统计力学：脑桥静脉损伤本构模型。

IF 2.3 4区生物学

European Biophysics Journal Pub Date : 2026-04-16 DOI: 10.1007/s00249-026-01839-2

Silvia García-Vilana, David Sánchez-Molina, Hamed Abdi, Vafa Rahimi-Movaghar

{"title":"Statistical mechanics of soft collagenous tissues: a constitutive model with damage for cerebral bridging veins","authors":"Silvia García-Vilana, David Sánchez-Molina, Hamed Abdi, Vafa Rahimi-Movaghar","doi":"10.1007/s00249-026-01839-2","DOIUrl":"10.1007/s00249-026-01839-2","url":null,"abstract":"<div>\u0000 \u0000 <p>A novel constitutive material model for the collagenous tissue of bridging veins is presented in this article. The walls of blood vessels are structurally reinforced by the collagen fibers, which are interconnected with an elastin matrix. Collagen fibers are oriented according to a specific probability distribution, with a greater abundance of fibers along the longitudinal axis. It is possible to analyze the collective mechanical behavior of collagen fibers using statistical mechanics techniques. The article describes how to derive the macroscopic behavior of collagenous tissue using the formalism of the canonical ensemble of statistical mechanics. One of the innovations of this formulation is the incorporation of a damage variable that increases significantly under excessive stretching. This formalism enables the derivation of a constitutive material model that is thermodynamically consistent, in which entropy increases irreversibly with the level of damage. In the model, an increase in damage results in a permanent increase in entropy. Furthermore, for a given level of damage, an increase in strain induces organization in the fibers, which is consistent with the behavior of an entropically elastic material. In addition, the thermodynamic properties of the model account for specific empirical observations, such as the slight contraction that occurs in a blood vessel when it is heated. The model’s mechanical suitability was assessed by subjecting it to uniaxial tensile tests on bridging veins. The findings provide strong evidence that the proposed constitutive model accurately reproduces the stress–strain curves (<span>(R^2_{adj}> 0.99)</span>).</p>\u0000 </div>","PeriodicalId":548,"journal":{"name":"European Biophysics Journal","volume":"55 4","pages":"679 - 687"},"PeriodicalIF":2.3,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00249-026-01839-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147697442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flow-induced dispersion analysis for probing cyclodextrin–guest binding interactions 流动诱导分散分析探测环糊精-客体结合相互作用。

IF 2.3 4区生物学

European Biophysics Journal Pub Date : 2026-04-15 DOI: 10.1007/s00249-026-01842-7

Josef Hamacek, Federico Perche

{"title":"Flow-induced dispersion analysis for probing cyclodextrin–guest binding interactions","authors":"Josef Hamacek, Federico Perche","doi":"10.1007/s00249-026-01842-7","DOIUrl":"10.1007/s00249-026-01842-7","url":null,"abstract":"<div>\u0000 \u0000 <p>Host–guest interactions between cyclodextrins and small molecules are fundamental in drug delivery, supramolecular chemistry, and molecular sensing. In this study, we applied Flow-Induced Dispersion Analysis (FIDA) to investigate the binding of a tryptophan derivative with α-, β-, and γ-cyclodextrins. For this purpose, L-tryptophan (<i>trp</i>) was fluorescently labelled with fluorescein (Fl-trp) to serve as a detectable indicator, enabling both direct binding and competitive displacement assays. Direct FIDA measurements revealed preferential binding of Fl-trp to β-cyclodextrin, with an apparent association constant K ~ 470 M<sup>− 1</sup>, while α- and γ-cyclodextrins showed weaker interactions, in agreement with literature. In competitive displacement experiments, the addition of unlabelled <i>trp</i> that binds to cyclodextrins effectively shifts the equilibria to the displacement of Fl-trp from the cyclodextrin cavities. The titration with [<i>trp</i>]<sub>tot</sub> allows to indirectly monitor the complex dissociation via changes in Rₕ. These results demonstrate that FIDA can be successfully applied to quantify small-molecule host–guest interactions, including weak or transient complexes, by tracking relative Rₕ changes. This approach provides a versatile, quantitative complement to conventional techniques such as NMR, ITC, or fluorescence titrations, and can be extended to other small-molecule binding systems in complex matrices.</p>\u0000 </div>","PeriodicalId":548,"journal":{"name":"European Biophysics Journal","volume":"55 :","pages":"413 - 418"},"PeriodicalIF":2.3,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147687636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing Italian biophysics: insights from the 27th SIBPA congress 推进意大利生物物理学：来自第27届SIBPA大会的见解。

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2026-04-11 DOI: 10.1007/s00249-026-01840-9

Silvia Caponi, Rita Carrotta, Paolo Bianchini, Velia Minicozzi, Alberto Diaspro

引用次数: 0

Indenting multicellular spheroids with various tip geometries 具有各种尖端几何形状的缩进多细胞球体。

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2026-04-06 DOI: 10.1007/s00249-026-01838-3

Kajangi Gnanachandran, Ewelina Lorenc, Alessandro Podestà, Małgorzata Lekka

{"title":"Indenting multicellular spheroids with various tip geometries","authors":"Kajangi Gnanachandran, Ewelina Lorenc, Alessandro Podestà, Małgorzata Lekka","doi":"10.1007/s00249-026-01838-3","DOIUrl":"10.1007/s00249-026-01838-3","url":null,"abstract":"<div>\u0000 \u0000 <p>Spheroids are of great interest in the study of cancer as they can partially mimic the tumour microenvironment, thus allowing to investigate several aspects of cell – microenvironment interactions in healthy and diseased conditions, including those pertaining to mechanobiology. Atomic Force Microscopy (AFM) is a versatile tool for studying biological samples and their mechanobiological properties. In AFM, the tip shape and dimensions determine the contact geometry between the tip and the sample and the length scales at which the mechanical properties are probed. Given the complex multiscale structure of spheroids, the choice of tip geometry and size would allow, in principle, to dissect the mechanical response of the overall system into the contributions of the constituents, from the single cell level to the cellular aggregate. In this work, we studied the mechanical properties of spheroids derived from four cell lines (A549, NHLF, HT-29, and CCD-18Co cells). Our studies revealed that using different contact geometries in the fitting procedure results in significantly different Young’s modulus values, highlighting the multiscale response of these complex cellular systems and the importance of a precise experimental design and choice of the AFM probe for the nanomechanical measurements. We observed that the location of F-actin filaments is correlated with the rigidity of the spheroids.</p>\u0000 </div>","PeriodicalId":548,"journal":{"name":"European Biophysics Journal","volume":"55 :","pages":"319 - 330"},"PeriodicalIF":2.4,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00249-026-01838-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0