Anthony F Craig, Livio Heath, Jan E Crafford, Juergen A Richt, Robert Swanepoel
{"title":"Updated distribution and host records for the argasid tick Ornithodoros (Pavlovskyella) zumpti: A potential vector of African swine fever virus in South Africa.","authors":"Anthony F Craig, Livio Heath, Jan E Crafford, Juergen A Richt, Robert Swanepoel","doi":"10.4102/ojvr.v88i1.1960","DOIUrl":"https://doi.org/10.4102/ojvr.v88i1.1960","url":null,"abstract":"<p><p>African swine fever virus (ASFV) causes a lethal and contagious disease of domestic pigs. In South Africa, the virus historically circulated in warthogs and ornithodorid ticks that were only found in warthog burrows in the north of the country. Regulations implemented in 1935 to prevent transfer of infected animals or products to the south initially proved effective but from 2016 there have been outbreaks of disease in the south that cannot be traced to transfer of infection from the north. From 1963 there were widespread translocations of warthogs to the south, initially from a source considered to be free of ornithodorid ticks. We undertook to determine whether sylvatic circulation of ASFV occurs in the south, including identification of potential new vectors, through testing extralimital warthogs for antibody and ticks for virus. Results of testing warthogs for antibody and other species of ticks for virus will be presented separately. Here we report finding Ornithodoros (Pavlovskyella) zumpti ticks in warthog burrows for the first time. This occurred in the Eastern Cape Province (ECP) in 2019. Since African swine fever was recognised in the ECP for the first time in 2020 and outbreaks of the disease in domestic pigs continue to occur there, priority should be given to determining the distribution range and vector potential of O. (P.) zumpti for ASFV.</p>","PeriodicalId":54685,"journal":{"name":"Onderstepoort Journal of Veterinary Research","volume":"88 1","pages":"e1-e4"},"PeriodicalIF":2.7,"publicationDate":"2021-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39595803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adama Sanou, Amadou Dicko, Kadiatou R Sow, Arthur Djibougou, Antoinette Kabore, Bassirou Diarra, Arsène K Ouedraogo, Dezemon Zingue, Moumini Nouctara, Zekiba Tarnagda
{"title":"Epidemiology and microscopic diagnosis of tuberculosis in pigs and small ruminants slaughtered at Bobo-Dioulasso abattoir, Burkina Faso.","authors":"Adama Sanou, Amadou Dicko, Kadiatou R Sow, Arthur Djibougou, Antoinette Kabore, Bassirou Diarra, Arsène K Ouedraogo, Dezemon Zingue, Moumini Nouctara, Zekiba Tarnagda","doi":"10.4102/ojvr.v88i1.1908","DOIUrl":"https://doi.org/10.4102/ojvr.v88i1.1908","url":null,"abstract":"<p><p>Bovine tuberculosis (bTB) is a zoonotic, infectious, chronic and contagious disease, caused by Mycobacterium bovis that mainly affects cattle. This pathology has a negative impact on animals and animal products trade. Unfortunately, in Burkina Faso where agriculture and livestock sectors represent around 80% of the socio-economic activities, the real situation of the disease is not well known especially in small ruminants and swine. Thus, our study focused on both the epidemiology and the microbiological diagnosis of tuberculosis (TB) in small ruminants and pigs slaughtered at Bobo-Dioulasso abattoir. A prospective study was conducted between August 2017 and December 2017. Epidemiological data collection was performed during routine meat inspection; moreover, samples were taken and transported to the Bacteriology laboratory of Centre Muraz for microbiological analyses. This diagnosis consisted in search of Acid Fast Bacilli (AFB) using the hot Ziehl-Neelsen staining. Out of a total of 14 648 small ruminants and 2430 pigs slaughtered during the study period, 156 and 17 had lesions suggestive of bTB with prevalence of 1.07% and 0.7%, respectively. Females and those between 2 and 4 years old were mainly infected. The most affected organs were: lungs, liver, spleen and lymph nodes. Finally, microscopy revealed 43.35% (75/173) of positive cases for AFB. These results confirm the presence of bTB in small ruminants and pigs in Burkina Faso. Efforts must still be made in the fight against this zoonosis in order to limit its economic and public health impacts.</p>","PeriodicalId":54685,"journal":{"name":"Onderstepoort Journal of Veterinary Research","volume":"88 1","pages":"e1-e6"},"PeriodicalIF":2.7,"publicationDate":"2021-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678950/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39580669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Opeyemi U Lawal, Abimbola O Adekanmbi, Olawale O Adelowo
{"title":"Occurrence of methicillin-resistant staphylococci in the pig-production chain in Ibadan, Nigeria.","authors":"Opeyemi U Lawal, Abimbola O Adekanmbi, Olawale O Adelowo","doi":"10.4102/ojvr.v88i1.1959","DOIUrl":"https://doi.org/10.4102/ojvr.v88i1.1959","url":null,"abstract":"<p><p>Staphylococcus species colonises humans and animals and is a major food contaminant with public health significance. Here, we assessed the occurrence of methicillin-resistant staphylococci (MRS) in the pig-production chain in Ibadan, Nigeria. Nares of 120 pigs and 10 farmers were sampled with sterile swabs whilst 54 pork samples were collected from a retail slaughterhouse. Staphylococcus species were isolated using enrichment, cefoxitin-aztreonam selective broth and Mannitol salt agar. Isolates were tested for susceptibility to cefoxitin (30 μg), oxacillin (1 μg) and vancomycin (30 μg). Methicillin-resistant staphylococci isolates were characterised using conventional biochemical tests. From 184 samples, 364 staphylococcal isolates were obtained. Amongst the 54 pork samples, 44.0% were contaminated with Staphylococcus species. Overall, 9 (2.5%) MRS were obtained and presumptively identified as Staphylococcus xylosus (n = 3), Staphylococcus sciuri (n = 3), Staphylococcus warneri (n = 2) and Staphylococcus cohnii (n = 1). There was no relationship between the prevalence of MRS between pigs and pig handlers in the farms, but Farm 2 had the highest frequency of 66.7% (p 0.05). Piglets had the highest prevalence of 66.7% (p 0.05) whilst MRS was absent in workers and pork samples. This study raises concerns about the cross-contamination of staphylococci in the food chain. Constant surveillance is imperative to ensure food safety.</p>","PeriodicalId":54685,"journal":{"name":"Onderstepoort Journal of Veterinary Research","volume":"88 1","pages":"e1-e4"},"PeriodicalIF":2.7,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8661277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39580668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Joanne Karzis, Inge-Marie Petzer, Vinny Naidoo, Edward F Donkin
{"title":"The spread and antimicrobial resistance of Staphylococcus aureus in South African dairy herds - A review.","authors":"Joanne Karzis, Inge-Marie Petzer, Vinny Naidoo, Edward F Donkin","doi":"10.4102/ojvr.v88i1.1937","DOIUrl":"https://doi.org/10.4102/ojvr.v88i1.1937","url":null,"abstract":"<p><p>Staphylococcus aureus is internationally recognised as a principal agent of mastitis and the foremost reason for economic loss in the dairy industry. The limited data available on organism-specific antibiotic resistance surveillance in dairy cattle have stimulated the need for such a review article. The objective of this study was to review relevant literature on antimicrobial resistance of mastitis-causing staphylococci isolated from dairy cows in South Africa compared to other countries. Factors relating to the incidence of mastitis and treatment strategies in terms of the One Health concept and food security were included. The Web of Science (all databases) and relevant websites were used, and articles not written in English were excluded. The incidence of mastitis varied between South Africa and other countries. Antimicrobial resistance patterns caused by S. aureus also varied in regions within Southern Africa and those of other countries although some similarities were shown. Antimicrobial resistance differed between S. aureus bacteria that were maltose positive and negative (an emerging pathogen). The results highlighted the importance of the availability of organism-specific surveillance data of the incidence of mastitis and antibiotic resistance for specific countries and within similar climatic conditions. Accurate knowledge about whether a specific pathogen is resistant to an antibiotic within a certain climate, country, area or farm should reduce the incidence of unnecessary or incorrect treatment with antibiotics. This should enable dairy farmers to deal with these organisms in a more effective manner. Therefore such research should be ongoing.</p>","PeriodicalId":54685,"journal":{"name":"Onderstepoort Journal of Veterinary Research","volume":"88 1","pages":"e1-e10"},"PeriodicalIF":2.7,"publicationDate":"2021-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8603139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39905907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ciza A Mushagalusa, Eric Etter, Mary-Louise Penrith
{"title":"Review of African swine fever outbreaks history in South Africa: From 1926 to 2018.","authors":"Ciza A Mushagalusa, Eric Etter, Mary-Louise Penrith","doi":"10.4102/ojvr.v88i1.1919","DOIUrl":"https://doi.org/10.4102/ojvr.v88i1.1919","url":null,"abstract":"<p><p>The article reviews the outbreaks and distribution of African swine fever (ASF) in South Africa since the first probable outbreak that occurred in the Koedoesrand Ward in 1926. Retrospective data on the ASF outbreaks in South Africa were obtained from the World Organisation for Animal Health (OIE) disease database and the South African veterinary services annual reports in addition to published articles and online sources. South Africa has experienced many outbreaks that can be divided into 2 time periods: the period before the development of the OIE diseases database (1993) and the period after. More than 141 outbreaks of ASF were reported during the first period. Since the development of OIE disease database, 72 outbreaks directly involving 2968 cases, 2187 dead and 2358 killed pigs mainly in smallholder pig farms were reported. The median number of cases for a given ASF outbreak is 17, but in 50% of outbreaks no pigs were killed for prevention. The most important ASF outbreak was reported in April 2014 in the Greater Zeerust district (North West province) involving 326 cases and 1462 killed pigs. However, the outbreak with highest mortality involving 250 pigs was reported in 2016 (Free State province). According to phylogenetic analysis, nine p72 genotypes (I, III, IV, VII, VIII, XIX, XX, XXI and XXII) have been identified in South Africa. Season-wise, more outbreaks were recorded during summer. It was also observed that the OIE disease database could contain errors that would have been introduced through compiled forms at country level. Spatiotemporal studies on ASF outbreaks in South Africa are therefore required in order to assess statistically and quantitatively the clustering of outbreaks over space and time.</p>","PeriodicalId":54685,"journal":{"name":"Onderstepoort Journal of Veterinary Research","volume":"88 1","pages":"e1-e10"},"PeriodicalIF":2.7,"publicationDate":"2021-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517827/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39507422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Samuel E Mantip, Anthony Sigismeau, Maurice Nanven, Atuman Joel, Abayomi M Qasim, Sada Aliyu, Ibrahim Musa, Ogechukwu Ezeanyika, Ibikunle Faramade, Garba Ahmed, Timothy Y Woma, David Shamaki, Genevieve Libeau, Souaibou Farougou, Arnaud Bataille
{"title":"Wide circulation of peste des petits ruminants virus in sheep and goats across Nigeria.","authors":"Samuel E Mantip, Anthony Sigismeau, Maurice Nanven, Atuman Joel, Abayomi M Qasim, Sada Aliyu, Ibrahim Musa, Ogechukwu Ezeanyika, Ibikunle Faramade, Garba Ahmed, Timothy Y Woma, David Shamaki, Genevieve Libeau, Souaibou Farougou, Arnaud Bataille","doi":"10.4102/ojvr.v88i1.1899","DOIUrl":"https://doi.org/10.4102/ojvr.v88i1.1899","url":null,"abstract":"<p><p>Peste des petits ruminants (PPR) is a highly contagious viral disease that mainly affects goats and sheep in Asia, Africa and the Middle East, and threatens Europe [R.E.1]. The disease is endemic on the African continent, particularly in West Africa, and is a major factor driving food insecurity in low-income populations. The aim of this research study was to carry out surveillance, genetic characterisation and isolation of recently circulating PPR viruses (PPRV) in sheep and goats from the six agro-ecological zones of Nigeria. A total of 268 post-mortem tissue samples of lung and mesenteric ganglia were collected from clinically suspected sheep and goats in 18 different states, of which five never previously sampled. The presence of PPRV was confirmed using a reverse-transcription coupled with a polymerase chain reaction (RT-PCR) assay. A total of 72 samples, 17 sheep (6%) and 55 goats (21%), were found to be PPR positive. Positive samples were distributed in almost all states, except Kano, where PPR was detected in previous studies. The PPRV-positive samples were further confirmed by sequencing or virus isolation in areas where the infection had never previously been detected. These results confirm the active circulation of PPRV across all six agro-ecological zones of Nigeria, and consequently, the need for introducing strict measures for the control and prevention of the disease in the country.</p>","PeriodicalId":54685,"journal":{"name":"Onderstepoort Journal of Veterinary Research","volume":"88 1","pages":"e1-e7"},"PeriodicalIF":2.7,"publicationDate":"2021-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39507421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"بررسی میزان شیوع و سیمای الکتروکاردیوگرافیک، پاتولوژیک و هماتولوژیک زخم شیردان گاو در کشتارگاه صنعتی سنندج","authors":"محمدسینا عباس زاده, شاهین فکور, لقمان اکرادی","doi":"10.22059/JVR.2020.295864.3014","DOIUrl":"https://doi.org/10.22059/JVR.2020.295864.3014","url":null,"abstract":"زمینۀ مطالعه: زخم شیردان یک بیماری گوارشی در نشخوارکنندگان به شمار میرود که متناسب با نوع آن نشانههای بالینی متفاوتی دارد؛ زیانهای اقتصادی زخم شیردان شامل کاهش تولید شیر و تلفات میباشد.هدف: تعیین شیوع زخم شیردان و نوع آن، تغییرات پاتولوژیک، الکتروکاردیوگرافی و هماتولوژی میباشد.روشکار: تعداد 400 رأس گاو در دو جنس و در گروههای سنی 2-1، 3-2، 4-3 و بیشتر از 4 سال در سه فصل سال بهصورت تصادفی انتخاب شدند پس از انجام الکتروکاردیوگرافی، خونگیری به عمل آمد و زخمهای شیردان، مورد مطالعه ماکروسکوپی و هیستوپاتولوژی قرار گرفتند.نتایج: مطالعه حاضر نشان داد که 51 مورد (75/12درصد) از کل نمونهها مبتلا به زخم شیردان بودند. تمام زخمها از نوع 1 و تحت نوعهای a1، b1، c1، d1 تشخیص داده شدند. نتایج الکتروکاردیوگرافی در 7/68 درصد موارد انواع آریتمی را نشان دادند که بیشترین نوع آنها آریتمی و تاکی کاردی سینوسی بود. نتایج آزمایشهای خونشناسی نوتروفیلی و کاهش MCHC را در گاوهای مبتلا به زخم شیردان بهصورت معنیداری نشان داد (05/0>p )، ارتباط معنیداری بین شیوع زخم شیردان با آریتمی، سن، جنس و فصل نشان داده شد. همچنین ارتباط آماری معنیداری میان تغییرات الکتروکاردیوگرافی و تغییرات هماتولوژی در گاوهای مبتلا مشاهده شد (05/0>p ).نتیجهگیری نهایی: نتایج مطالعه حاضر شیوع بالای زخم شیردان را در منطقه نشان داد و همچنین بیان کرد که تغییرات الکتروکاردیوگرافی و خونشناسی میتوانند درکنارسایر یافتهها به تشخیص بیماری کمک کنند.","PeriodicalId":54685,"journal":{"name":"Onderstepoort Journal of Veterinary Research","volume":"1 1","pages":"216-224"},"PeriodicalIF":2.7,"publicationDate":"2021-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90199265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"تعیین سویه کیستهای هیداتید گوسفندان و بزهای کشتار شده در کشتارگاه بیرجند با روشهای مورفولوژیکی و مولکولی با استفاده از ژن ITS1","authors":"سوسن انصاری, حسن برجی, ابوالقاسم نقیبی","doi":"10.22059/JVR.2020.299752.3037","DOIUrl":"https://doi.org/10.22059/JVR.2020.299752.3037","url":null,"abstract":"زمینۀ مطالعه: اکینوکوکوزیس یکی از مهمترین بیماریهای مشترک بین انسان و دام در نواحی مختلف ایران است. درحالیکه این بیماری یک معضل بهداشتی مهم در انسان میباشد اطلاعات محدودی در مورد چرخه انتقال و سویههای این انگل در مناطق شرق ایران وجود دارد. \u0000هدف: در مطالعه حاضر تعیین هدف اصلی سویههای کیست هیداتید گوسفندان و بزهای کشتار شده در کشتارگاه بیرجند با روش مورفولوژی و مولکولی است. \u0000روشکار: تعداد 30 عدد کیست هیداتید از گوسفند و تعداد 30 عدد کیست هیداتید از بزهای کشتارگاه بیرجند جمعآوری گردید و با استفاده از روش مورفولوژی (شمارش تعداد و اندازهگیری طول قلابها) و روش مولکولی (PCR-RFLP of ITS1) اقدام به تعیین سویه کیست هیداتید گردید. همچنین قطعاتی از ژن ITS تعیین توالی گردید. \u0000نتایج: در بین دوسویه مشخص و متفاوت گوسفندی و شتری، سویه گوسفندی شایعترین سویه در بین کیستهای هیداتید گوسفند و بز بود. کل کیستهای جداشده از گوسفندان و تعداد 20 کیست جداشده از بزها آلوده به سویه گوسفندی (G1-G3) بودند. درحالیکه سویه شتری فقط از بز جدا شد و تعداد 10 کیست از مجموع 30 کیست آلوده به سویه شتری (G6/G7) بود. سویه شتری الگوی برش آنزیمی متفاوت با سویه گوسفندی نشان داد. \u0000نتیجهگیری نهایی: نتایج مطالعه حاضر نشان داد که سویه گوسفندی (G1) شایعترین سویه در گوسفندان و بزهای کشتار شده در این منطقه باشد. علاوه بر این،حضور سویه شتری (G6) در بزهای کشتار شده در بیرجند مورد تأیید قرار میگیرد.","PeriodicalId":54685,"journal":{"name":"Onderstepoort Journal of Veterinary Research","volume":"23 1","pages":"146-153"},"PeriodicalIF":2.7,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78183656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"بررسی مورفولوژیکی و مولکولی ککهای کتنوسفالیدس جدا شده از سگهای ولگرد شهر تهران","authors":"سارا آزادبه, عبدالحسین دلیمی, شهرام جمشیدی","doi":"10.22059/JVR.2020.227440.2586","DOIUrl":"https://doi.org/10.22059/JVR.2020.227440.2586","url":null,"abstract":"زمینۀ مطالعه: تاکنون گونههای مختلفی از انگلهای خارجی از سگ گزارش شدهاند که در این میان ککها از اهمیت قابل توجهی برخوردارند و شناسایی صحیح آنها میتواند به لحاظ بهداشتی ما را در کنترل این انگل خارجی یاری کند. \u0000هدف: با توجه به عدم شناسایی دقیق ککها، در مطالعه حاضر سعی در شناسایی ککهای کتنوسفالیدس به دو روش مورفولوژیکی و مولکولی شده است. \u0000روشکار: در مطالعه حاضر ابتدا سگهای ارجاع شده به بیمارستان دامپزشکی دانشگاه تهران مورد بررسی قرار گرفتند، سپس نسبت به جمع آوری و شناسایی ککهای جدا شده از سگها اقدام شد. شناسایی مورفولوژیک توسط میکروسکوپ و کلیدهای تشخیصی انجام شد و مطالعه مولکولی با استفاده از روش PCR و تکثیر ژن COX1 ککهای کتنوسفالیدس انجام شد. در مرحله بعد شناسایی با استفاده از آزمایش RFLP و تعیین ترادف بازی صورت گرفت. \u0000نتایج: در مطالعه حاضر از20 قلاده سگ آلوده به کک، 605 عدد کک جداسازی شد. در مطالعات مورفولوژیک، سه گونه کتنوسفالیدس فلیس، کتنوسفالیدس کنیس و پولکس ایریتانس شناسایی گردید. از لحاظ مولکولی نیز قطعهای به طول 552 جفت بازی از ژن COX1 ککهای کتنوسفالیدس تکثیر و روی ژل ظاهر شد. پس از تعیین توالی، مشخص گردید که در توالی جایگاه ژنی COX1، دو گونه کتنوسفالیدس فلیس وکتنوسفالیدس کنیس 99 درصد مشابهت وجود دارد. در آزمایش PCR-RFLP که از آنزیم Taq1 برای تفکیک دو گونه استفاده شد، نتایج برای هر دو گونه مشابه بود. \u0000نتیجهگیری نهایی: گرچه این دو گونه کک سگ از لحاظ مورفولوژی قابل تفکیک میباشند ولی تشخیص مولکولی آنها با استفاده از ژنهای COX1 موفقیتآمیز نبوده است.","PeriodicalId":54685,"journal":{"name":"Onderstepoort Journal of Veterinary Research","volume":"13 1","pages":"154-161"},"PeriodicalIF":2.7,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85245002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"بررسی مقایسهای پروفایل بیان ژنهای MHC-I، CD90 و CD29 در سلولهای بنیادی مزانشیمی گرفته شده از مغزاستخوان و بافت چربی اسب","authors":"فاطمه نظری, عباس پرهام, ادهم فانی ملکی","doi":"10.22059/JVR.2020.293443.2997","DOIUrl":"https://doi.org/10.22059/JVR.2020.293443.2997","url":null,"abstract":"زمینۀ مطالعه: تاکنون مطالعات بسیاری بر روی سلولهای بنیادی مزانشیمی به عنوان راه درمان بیماریهای اسکلتی – عضلانی در اسب انجام شده است. مغز استخوان و بافت چربی منابع اصلی جداسازی سلولهای بنیادی مزانشیمی در اسب بودهاند. علیرغم وجود شباهت زیاد سلولهای این دو منبع، تفاوتهایی نیز در ویژگیهای زیستشناختی، تکثیری، تحریک ایمنی و تمایزی آنها وجود دارد و هنوز توافقی بر سر اینکه کدام منبع برای ایجاد بانک سلولهای بنیادی مزانشیمی آلوژن مناسبتر است، وجود ندارد. ازآنجاییکه برخی از این تفاوتها در سطح بیان ژن قابلردیابی هستند، استفاده از روش آنالیز کمی بیان ژن (qPCR) در شناسایی بهتر این سلولها و مقایسه این منابع توصیه میشود. \u0000هدف: بررسی مقایسهای میزان رونوشت ژنهای Major Histocompatibility Complex-I (MHC-I)، Cluster of Differentiation-90 (CD90) و CD29 در سلولهای بنیادی مزانشیمی بافت چربی و مغزاستخوان اسب که به ترتیب ژنهای مؤثر در ایمنیزایی و نشانگرهای شناسایی مؤثر در استئوژنز و لانهگزینی در این سلولها میباشند. \u0000روشکار: برای این منظور، سلولهای بنیادی مزانشیمی بافت چربی و مغز استخوان 3 رأس اسب مورد استفاده قرار گرفت. RNA تام سلولهای پاساژ سوم استخراج گردید و پس از سنتز cDNA، میزان بیان ژنهای مورد بررسی، با روش qPCR مورد ارزیابی قرار گرفت. \u0000نتایج: بر اساس نتایج این مطالعه مشخص شد که سلولهای بنیادی مزانشیمی بافت چربی میزان برابری از ژن MHC-I و میزان بیشتری از ژنهای CD90 و CD29 را در مقایسه با سلولهای بنیادی مزانشیمی مغزاستخوان بیان میکنند که در خصوصیات بیولوژیک آنها از جمله در مهاجرت و توان تمایزی تأثیر گذار میباشند. \u0000نتیجهگیری نهایی: بر اساس نتایج به دست آمده، سلولهای بنیادی مزانشیمی گرفته شده از مغزاستخوان و بافت چربی اسب از نظر الگوی بیان ژنی تفاوتهایی را نشان میدهند که مؤید تفاوتهای عملکردی آنها میباشد. تفاوتهایی که باید در درمانهای مبتنی بر سلول مورد توجه قرار گیرند.","PeriodicalId":54685,"journal":{"name":"Onderstepoort Journal of Veterinary Research","volume":"7 1","pages":"251-260"},"PeriodicalIF":2.7,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75723415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}