{"title":"Unraveling MicroRNA Mediated Gene Regulation in Centella Asiatica (L.) Urb. by High-Throughput Sequencing based Small-RNA Profiling.","authors":"Gouri Priya Ranjith, Jisha Satheesan, K. Sabu","doi":"10.21203/rs.3.rs-995211/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-995211/v1","url":null,"abstract":"\u0000 Centella asiatica is a widely spread herb mostly found in the tropics having extensive medicinal values. Here, we report for the first time, transcriptome-wide characterization of miRNA profile from the leaves of C. asiatica using high-throughput Illumina sequencing. We identified 227 conserved and 109 putative novel miRNAs. Computational screening revealed potential mRNA targets for both the conserved and novel miRNAs encoding diverse transcription factors and enzymes involved in plant development, disease resistance, metabolic and signaling pathways. Gene ontology annotation and KEGG analysis revealed the miRNA targets to be involved in a wide range of metabolomic and regulatory pathways. The differential expression of the miRNA encoding genes in diverse tissues was determined by real-time PCR analysis. We also found that gene expression levels of miR156, 159 and 1171 was reduced in salicylic acid treated axenic shoot cultures of C. asiatica compared to its control. Furthermore, RLM-RACE experiments mapped miRNA-mediated cleavage at two of the mRNA targets. The present study represents the large-scale identification of microRNAs from C. asiatica and contributes to the base for the up-coming studies on miRNA-mediated gene regulation of plant secondary metabolite pathways in particular.","PeriodicalId":54356,"journal":{"name":"Tropical Plant Biology","volume":"1 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2021-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43384686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Garcia, Marcelo Rodrigues, J. Pennacchi, Ane Marcela das Chagas Mendonça, Daynara Aparecida Rodrigues Gonçalves, Nayara Cristina de Melo, J. P. R. A. D. Barbosa
{"title":"Sugarcane Resilience to Recurrent Water Deficit is Dependent on the Systemic Acclimation of Leaf Physiological Traits","authors":"F. Garcia, Marcelo Rodrigues, J. Pennacchi, Ane Marcela das Chagas Mendonça, Daynara Aparecida Rodrigues Gonçalves, Nayara Cristina de Melo, J. P. R. A. D. Barbosa","doi":"10.1007/s12042-021-09303-5","DOIUrl":"https://doi.org/10.1007/s12042-021-09303-5","url":null,"abstract":"","PeriodicalId":54356,"journal":{"name":"Tropical Plant Biology","volume":"14 1","pages":"408 - 418"},"PeriodicalIF":2.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48823161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lijuan Yang, Shifang Li, Zimeng Zhang, Meiguang Lu
{"title":"Genome-Wide Identification of MicroRNAs that are Responsive to Virus/Viroid Infection in Nectarine Trees Through High-Throughput Sequencing","authors":"Lijuan Yang, Shifang Li, Zimeng Zhang, Meiguang Lu","doi":"10.1007/s12042-021-09300-8","DOIUrl":"https://doi.org/10.1007/s12042-021-09300-8","url":null,"abstract":"","PeriodicalId":54356,"journal":{"name":"Tropical Plant Biology","volume":"15 1","pages":"78 - 92"},"PeriodicalIF":2.0,"publicationDate":"2021-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48790802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of Genome-wide SSR Markers for Physical Map Construction with PCR-based Polymorphic SSRs in Jute (Corchorus Spp.)","authors":"Niyitanga, Sylvain, Yao, Jiayu, Ibrahim, Aminu kurawa, Afzal, Muhammad Zohaib, Chen, Siyuan, Zhang, Liwu","doi":"10.1007/s12042-021-09301-7","DOIUrl":"https://doi.org/10.1007/s12042-021-09301-7","url":null,"abstract":"<p>Despite substantial efforts in the past decades towards the development of microsatellites or SSR (simple sequence repeat) markers in jute, there is still an urgent necessity for additional SSR markers for performing genetic and breeding investigations. The availability of reference genomic sequences for both diploid cultivated jute species enabled us to identify a total of 154,715 and 160,173 SSRs from the <i>C. capsularis</i> and <i>C. olitorius</i> genomes, respectively. 6,337 and 7,012 total SSRs with a density of 187.6 and 185.01 SSR/Mb were also mined in coding sequences (CDS). 12bp was the most common repeat length in both genomic and CDS. Di- and tetra-nucleotide motifs were prevailed in the genomic sequences, while trinucleotides in the CDS. SSR frequency declined with increasing repeat units in both species. ATT and AT motifs were the most common in the genome. AG and CTT were prevalent in the CDS of both species while GC and CGC motifs were infrequent. Further, we used the flanking sequences of identified SSRs (from both species) to design a total of 1,117 SSR primer pairs. Genetic diversity analysis based on 110 sampled SSRs in 24 genotypes displayed high polymorphism. The first physical map with 535 PCR-based polymorphic SSRs were constructed by taking <i>C. capsularis</i> as the reference genome. These resources will advance genetics and breeding research in jute.</p>","PeriodicalId":54356,"journal":{"name":"Tropical Plant Biology","volume":"14 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2021-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138542081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genome-Wide Identification of WUSCHEL-Related Homeobox Gene Family and their Expression Analysis During Somatic Embryogenesis in Oil Palm (Elaeis guineensis)","authors":"Zhang, Anni, Li, Yinchun, Yarra, Rajesh, Li, Rui, Cao, Hongxing, Jin, Longfei","doi":"10.1007/s12042-021-09299-y","DOIUrl":"https://doi.org/10.1007/s12042-021-09299-y","url":null,"abstract":"<p><i>WUSCHEL</i>-related homeobox (<i>WOX</i>) genes have been shown to play important roles in the regulation of gene transcription involved in the early phases of embryogenesis. In this study, a total of 15 <i>EgWOXs</i> were identified from the oil palm genome and their physicochemical properties were analyzed. Phylogenetic analysis divided 15 EgWOXs into three different clades i.e. modern, ancient, and intermediate. EgWOXs contain ten conserved motifs and two conserved domains. Further, cis-acting elements analysis identified the presence of light, stress, and phytohormone responsive elements in the promoter regions of <i>EgWOX</i>s. RNA-seq analysis of <i>EgWOX</i> genes in different embryogenesis stages of oil palm embryogenic callus (EC), non-embryogenic callus (NEC), and somatic embryo (SE), indicated the significant expression of <i>EgWOXs</i> in EC and SE stages. Real-time PCR analysis results showed the significant expression of <i>EgWOX2</i>, <i>EgWOX7</i>, <i>EgWOX8</i>, <i>EgWOX9</i>, and <i>EgWOX12</i> genes in somatic embryogenesis stage, indicating their roles in regulating oil palm somatic embryogenesis.</p>","PeriodicalId":54356,"journal":{"name":"Tropical Plant Biology","volume":"351 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2021-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138508401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}