Genome-Wide Identification of WUSCHEL-Related Homeobox Gene Family and their Expression Analysis During Somatic Embryogenesis in Oil Palm (Elaeis guineensis)

IF 1.8 4区生物学 Q2 PLANT SCIENCES

Tropical Plant Biology Pub Date : 2021-09-06 DOI:10.1007/s12042-021-09299-y

Zhang, Anni, Li, Yinchun, Yarra, Rajesh, Li, Rui, Cao, Hongxing, Jin, Longfei

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引用次数: 3

Abstract

WUSCHEL-related homeobox (WOX) genes have been shown to play important roles in the regulation of gene transcription involved in the early phases of embryogenesis. In this study, a total of 15 EgWOXs were identified from the oil palm genome and their physicochemical properties were analyzed. Phylogenetic analysis divided 15 EgWOXs into three different clades i.e. modern, ancient, and intermediate. EgWOXs contain ten conserved motifs and two conserved domains. Further, cis-acting elements analysis identified the presence of light, stress, and phytohormone responsive elements in the promoter regions of EgWOXs. RNA-seq analysis of EgWOX genes in different embryogenesis stages of oil palm embryogenic callus (EC), non-embryogenic callus (NEC), and somatic embryo (SE), indicated the significant expression of EgWOXs in EC and SE stages. Real-time PCR analysis results showed the significant expression of EgWOX2, EgWOX7, EgWOX8, EgWOX9, and EgWOX12 genes in somatic embryogenesis stage, indicating their roles in regulating oil palm somatic embryogenesis.

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油棕(Elaeis guineensis) wuschell相关同源盒基因家族的全基因组鉴定及体细胞胚胎发生过程中的表达分析

wuschell相关同源盒(WOX)基因在胚胎发生早期参与的基因转录调控中发挥重要作用。本研究从油棕基因组中共鉴定出15个EgWOXs，并对其理化性质进行了分析。系统发育分析将15个egwox分为现代、古代和中间三个不同的支系。EgWOXs包含10个保守基序和2个保守结构域。此外，顺式作用元件分析发现，EgWOXs的启动子区域存在光、胁迫和植物激素响应元件。EgWOX基因在油棕胚性愈伤组织(EC)、非胚性愈伤组织(NEC)和体胚(SE)不同胚发生阶段的RNA-seq分析表明，EgWOX基因在EC和SE阶段均有显著表达。实时荧光定量PCR分析结果显示，EgWOX2、EgWOX7、EgWOX8、EgWOX9和EgWOX12基因在体胚发生阶段显著表达，提示其在调控油棕体胚发生中发挥作用。

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来源期刊

Tropical Plant Biology PLANT SCIENCES-

CiteScore

3.70

自引率

0.00%

发文量

期刊介绍： Tropical Plant Biology covers the most rapidly advancing aspects of tropical plant biology including physiology, evolution, development, cellular and molecular biology, genetics, genomics, genomic ecology, and molecular breeding. It publishes articles of original research, but it also accepts review articles and publishes occasional special issues focused on a single tropical crop species or breakthrough. Information published in this journal guides effort to increase the productivity and quality of tropical plants and preserve the world’s plant diversity. The journal serves as the primary source of newly published information for researchers and professionals in all of the aforementioned areas of tropical science.