LabmedicinePub Date : 2014-05-01DOI: 10.1309/LMD0YIFPTOWZONAD
R. Agarwal
{"title":"Quality-Improvement Measures as Effective Ways of Preventing Laboratory Errors","authors":"R. Agarwal","doi":"10.1309/LMD0YIFPTOWZONAD","DOIUrl":"https://doi.org/10.1309/LMD0YIFPTOWZONAD","url":null,"abstract":"Laboratory error is defined as any defect from ordering tests to reporting and interpretation of results. Laboratory errors have a reported frequency of 0.012-0.6% of all test results which in turn has huge impact on diagnosis and patient management as 60–70% of all diagnosis are made on the basis of laboratory tests. Total testing process in the laboratory is a cyclical process divided into three phases: preanalytical, analytical and postanalytical. First, pre-analytical phase in which requirement for a test is determined, the test is ordered and the patient is identified. It is followed by specimen collection and transport to the laboratory. The specimen is prepared and tested in the analytical phase. During the postanalytical phase, the results are reported to the individual who ordered the test and any action or intervention is undertaken. Initially, the policies and procedures developed by the laboratory were more concerned on analytical phase to reduce errors during laboratory testing and emphasis was in ensuring proper calibration and testing. The last few decades have seen a significant decrease in the rates of analytical errors in clinical laboratories. Currently, available evidences demonstrate that the pre- and postanalytical steps are more error prone.\u0000\u0000* IOM\u0000 : Institute of Medicine\u0000 NABL\u0000 : National Accreditation Board for Testing and Calibration Laboratories\u0000 CAP\u0000 : College of American Pathologists\u0000 JCI\u0000 : The Joint Commission International\u0000 QC\u0000 : quality control\u0000 COES\u0000 : computerized order entry system\u0000 CLSI\u0000 : Clinical and Laboratory Standards Institute\u0000 RFID\u0000 : radio frequency identification\u0000 OCR\u0000 : optical character recognition\u0000 TTP\u0000 : thrombotic thrombocytopenic purpura\u0000 EQAS\u0000 : External Quality Assurance Program\u0000 CLIA\u0000 : Clinical Laboratory Improvement Amendments\u0000 IQC\u0000 : interlaboratory QC\u0000 WHO\u0000 : World Health Organization\u0000 ATE\u0000 : allowable total error\u0000 POCT\u0000 : point-of-care testing","PeriodicalId":54328,"journal":{"name":"Labmedicine","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73167531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LabmedicinePub Date : 2014-05-01DOI: 10.1309/LMM0VDJ4EA8HUUWX
S. Wallace
{"title":"Building a Molecular Diagnostics Department","authors":"S. Wallace","doi":"10.1309/LMM0VDJ4EA8HUUWX","DOIUrl":"https://doi.org/10.1309/LMM0VDJ4EA8HUUWX","url":null,"abstract":"My name is Stephanie Wallace, and I am a lead technologist in the microbiology department at the OSF Saint Francis Medical Center (SFMC) System Laboratory in Peoria, IL. I am native to the central Illinois area and am pleased to work in an atmosphere in which I can serve my community. I received my undergraduate education at Illinois State University and received my master’s degree in training and development at the University of St. Francis. I have worked in the microbiology department for a little longer than 10 years.\u0000\u0000As a student going through clinical rotations, I enjoyed most areas of the clinical laboratory, but microbiology was the area of my strongest interest. I started working as a 2nd-shift microbiology technologist with OSF SFMC in 2002. Then, in 2004, I took my current position as a 1st-shift microbiology lead technologist. In addition … \u0000\u0000[↵][1]* To whom correspondence should be addressed. E-mail: stephanie.e.wallace{at}osfhealthcare.org\u0000\u0000 [1]: #xref-corresp-1-1","PeriodicalId":54328,"journal":{"name":"Labmedicine","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77417621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LabmedicinePub Date : 2014-05-01DOI: 10.1309/LMYZ114PTWEBDZPT
S. Wallace
{"title":"Creating a Molecular Atmosphere: Phase 1","authors":"S. Wallace","doi":"10.1309/LMYZ114PTWEBDZPT","DOIUrl":"https://doi.org/10.1309/LMYZ114PTWEBDZPT","url":null,"abstract":"The first phase of creating the molecular section in Microbiology started in late 2005 and early 2006, when we decided to bring in an analyzer that could perform real-time PCR assays. At that time, we were specifically interested in performing herpes simplex virus (HSV) typing but we wanted an analyzer that could accommodate a growing test menu. We chose the HSV to start because of its value to our pediatric infectious disease physician and its usefulness to other health care professionals. At the time, we performed virus cultures for HSV in-house and we sent out cerebrospinal fluid for HSV typing by polymerase chain reaction (PCR); the turnaround times were 2 to 4 days and 5 to 7 days, respectively. Offering this assay in-house would positively affect patient care by decreasing the turnaround time to 24 to 36 hours.\u0000\u0000Before creating a molecular section in our department, I had verified a qualitative assay approved by the United States Food and Drug Administration (FDA; running samples, performing statistical analysis, and completing a … \u0000\u0000[↵][1]* To whom correspondence should be addressed. E-mail: stephanie.e.wallace{at}osfhealthcare.org\u0000\u0000 [1]: #xref-corresp-1-1","PeriodicalId":54328,"journal":{"name":"Labmedicine","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73990049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LabmedicinePub Date : 2014-05-01DOI: 10.1309/LMLKRWWJNP6BXA32
S. Wallace
{"title":"Creating a Molecular Environment, Phase 4","authors":"S. Wallace","doi":"10.1309/LMLKRWWJNP6BXA32","DOIUrl":"https://doi.org/10.1309/LMLKRWWJNP6BXA32","url":null,"abstract":"When implementing molecular testing in the microbiology laboratory at OSF Saint Francis Medical Center, we started with the more difficult laboratory-developed testing and then moved to the United States Food and Drug Administration (FDA)–approved assays, which are equally important but require a less rigorous evaluation process. FDA-approved molecular testing can be less difficult because the assays in question have already been developed and validated. Our responsibility is to make sure that these assays work for our laboratory, write up the protocol with our specifications, and perform training before we turn out results on patients. Using FDA-approved testing usually involves following certain guidelines, which takes most of the guesswork out of the evaluation process.\u0000\u0000In 2007, the Illinois legislature passed a law known as the MRSA Screening and Report Act, which required all hospitals in Illinois to implement screening criteria for patients to determine whether they are carriers of methicillin-resistant Staphylococcus aureus (MRSA) on admission. This did not involve all patients but included those that were, according to the MRSA Screening and Report Act, “in all intensive care units, and other at-risk patients identified by the hospital”.\u0000\u0000OSF established guidelines for screening that have change slightly over the years. The most current screening criteria are as follows:\u0000\u0000[↵][1]* To whom correspondence should be addressed. E-mail: stephanie.e.wallace{at}osfhealthcare.org\u0000\u0000 [1]: #xref-corresp-1-1","PeriodicalId":54328,"journal":{"name":"Labmedicine","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81302764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LabmedicinePub Date : 2014-05-01DOI: 10.1309/LMS0JFCHA7DMQC0M
C. Cunningham
{"title":"Prezi Presentational Software as an Educational Tool for Analyzing Pathology Slides: Promoting a Learner Centered Environment Through Technology to Increase Student Satisfaction","authors":"C. Cunningham","doi":"10.1309/LMS0JFCHA7DMQC0M","DOIUrl":"https://doi.org/10.1309/LMS0JFCHA7DMQC0M","url":null,"abstract":"Objective: To test Prezi online presentational software as a learning tool for students enrolled in a physiopathology laboratory class.\u0000\u0000Methods: A Prezi presentation was created and used in place of a virtual microscopy scanning digital pathology slide during a laboratory session attended by a total of 179 students. Review of the normal anatomical features and architecture of a tissue was followed by an annotated tour covering important aspects of its pathologic characteristics. This was also accompanied by comparisons of normal with abnormal tissue to facilitate student learning and comprehension. Student feedback was gathered for 3 successive trimesters using a survey to assess student satisfaction with use of the Prezi presentation as an effective teaching strategy in a physiopathology laboratory class.\u0000\u0000Results: Students reported that they preferred the Prezi presentation compared with the digital pathology scanning slide technology. Students overwhelmingly expressed the desire to shift to the Prezi format from the virtual scanning microscope as the type of instructional technology used in class.\u0000\u0000Conclusion: Based on student feedback, Prezi is an effective teaching tool. The overall success of this exercise and the positive student feedback warrant further development of additional Prezi presentations for continued use in physiopathology laboratory classes.","PeriodicalId":54328,"journal":{"name":"Labmedicine","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85017008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LabmedicinePub Date : 2014-05-01DOI: 10.1309/LM8BLDCRH6WT0VPP
S. Aesif, M. Swierzbinski, J. Keiser
{"title":"Positive Blood Culture Results After Plasmodium falciparum Diagnosis","authors":"S. Aesif, M. Swierzbinski, J. Keiser","doi":"10.1309/LM8BLDCRH6WT0VPP","DOIUrl":"https://doi.org/10.1309/LM8BLDCRH6WT0VPP","url":null,"abstract":"We describe a case of falsely positive blood culture results due to the presence of Plasmodium falciparum in the cultured blood specimen. Although awareness among health care professionals of this phenomenon has been reported in the literature, we discovered that attending physicians and house staff at our institution were largely unaware of this phenomenon. Similarly, laboratory staff and technologists therein had little experience identifying this organism on Gram-stained blood smears. We believe that this report will serve as a useful reminder to some readers and will broaden the differential diagnoses generated by others.\u0000\u0000* WBCs\u0000 : white blood cells\u0000 RBCs\u0000 : red blood cells","PeriodicalId":54328,"journal":{"name":"Labmedicine","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89208012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LabmedicinePub Date : 2014-02-01DOI: 10.1309/LM24GWIUEXOKN7AP
W. Arneson
{"title":"Electrolytes: The Salts of the Earth","authors":"W. Arneson","doi":"10.1309/LM24GWIUEXOKN7AP","DOIUrl":"https://doi.org/10.1309/LM24GWIUEXOKN7AP","url":null,"abstract":"What do you need to know as medical laboratory scientists when performing plasma electrolyte analysis? What new information should you consider regarding best laboratory practice in electrolyte analysis? This review article will answer some of these questions. Background In human physiology it is best to refer to electrolytes, the main component in bodily fluids, in the context of extracellular fluid (ECF) volume and total water (H 2 O) content in the body. Electrolytes are also best referenced collectively rather than individually because they are part of an integrated physiological mechanism of H 2 O and ionic balance. Thirst, renal function, and hormonal response help to maintain homeostasis of electrolytes. 1 These entities play a role in general functions of metabolic pathways, enzyme activation, acid-base balance, muscular-function regulation, and nervous-tissue contractions. Control of electrolyte levels is based on H 2 O and pH balance and is enacted by the renal glands through processes such as active transport in the proximal convoluted tubules, osmosis, and passive diffusion. At a cellular level, sodium (NA) and potassium (K) levels are maintained by the Na-K–adenosine triphosphatase (ATPase) pump. 1 The endocrine system influences the distal convoluted tubules via the renin-aldosterone system and the circulating levels of vasopressin and natriuretic peptides in bodily fluids.","PeriodicalId":54328,"journal":{"name":"Labmedicine","volume":"71 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85902015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LabmedicinePub Date : 2014-02-01DOI: 10.1309/LMBR83WM3GNJEDLS
Muneeza Esani
{"title":"The Physiological Sources of, Clinical Significance of, and Laboratory-Testing Methods for Determining Enzyme Levels","authors":"Muneeza Esani","doi":"10.1309/LMBR83WM3GNJEDLS","DOIUrl":"https://doi.org/10.1309/LMBR83WM3GNJEDLS","url":null,"abstract":"Enzymes are organic molecules that accelerate biochemical reactions but emerge from the reaction unchanged. Abnormal levels of plasma enzymes are highly suggestive of damaged cells and provide clues to parts of the body that may be involved in disease processes. Enzyme levels are measured in the clinical laboratory to identify the site of damage and to quantify the amount of damage. This paper will discuss the physiological sources, clinical significance, and laboratory- testing methodology for lactate dehydrogenase (LD), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and gamma-glutamyltransferase (GGT).","PeriodicalId":54328,"journal":{"name":"Labmedicine","volume":"74 1","pages":"16-18"},"PeriodicalIF":0.0,"publicationDate":"2014-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79247196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LabmedicinePub Date : 2014-02-01DOI: 10.1309/LM3FXGAJ6S8TKRWK
D. Ernst
{"title":"Phlebotomy Worktext and Procedure Manual, 3rd Edition","authors":"D. Ernst","doi":"10.1309/LM3FXGAJ6S8TKRWK","DOIUrl":"https://doi.org/10.1309/LM3FXGAJ6S8TKRWK","url":null,"abstract":"Edited by Robin S Warekois, BS, MT(ASCP) and Richard Robinson 362 pages. St. Louis, Elsevier Saunders, 2012. $73.95. ISBN: 9781437709919 \u0000\u0000Instructors who purchase the newest edition of the Phlebotomy Worktext and Procedure Manual will find it to be a familiar resource. Retained in this edition are the hallmarks that have made this text stand out in the sea of competing titles published since the first edition was released in 2002. Such hallmarks include the logical organization of material, the detailed and well-written content, and the eye-pleasing graphics. This edition contains new images of needles replacing images of those without safety features, 50 additional mock examination questions, and certain updated passages to reflect some but not all provisions of the Clinical and Laboratory Standards Institute (CLSI) venipuncture standard, which was revised in 2007. Not much is new in this edition; despite certain issues concerning its content, as I will describe later in this review, it remains one of the more accurate texts on the subject in most regards.\u0000\u0000\u0000\u0000The organization of the material is logical; the content flows well, making it easy for educators to incorporate the contents into their curricula in the order in which they are presented. …","PeriodicalId":54328,"journal":{"name":"Labmedicine","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81870647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LabmedicinePub Date : 2014-02-01DOI: 10.1309/LMBW4EKPG1TMCTYO
C. Gebhart
{"title":"Molecular Microbiology: Diagnostic Principles and Practice, 2nd edition","authors":"C. Gebhart","doi":"10.1309/LMBW4EKPG1TMCTYO","DOIUrl":"https://doi.org/10.1309/LMBW4EKPG1TMCTYO","url":null,"abstract":"Edited by David H. Persing, Fred C. Tenover, Yi-Wei Tang, Frederick S. Nolte, Randall T. Hayden, and Alex Van Belkum 960 pages. Washington, DC: ASM Press, 2011. $189.95 ISBN: 9781555814977\u0000\u0000I found Molecular Microbiology: Diagnostic Principles and Practice, 2nd Edition to be an excellent resource for laboratory professionals and the clinical care team. This book provides chapter-by-chapter detail regarding the various molecular techniques that have been incorporated into use in microbiology laboratories during the past …","PeriodicalId":54328,"journal":{"name":"Labmedicine","volume":"1 1","pages":"8-8"},"PeriodicalIF":0.0,"publicationDate":"2014-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83757197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}