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The complete mitochondrial genome sequence from the longicorn beetle Obrium sp. (Coleoptera: Cerambycidae) 天牛甲虫(鞘翅目:天牛科)线粒体全基因组序列
4区 生物学
Mitochondrial Dna Part a Pub Date : 2017-05-01 DOI: 10.3109/19401736.2015.1122766
Nan Song, Hao Zhang, Xinming Yin, Aili Lin, Qing Zhai
{"title":"The complete mitochondrial genome sequence from the longicorn beetle Obrium sp. (Coleoptera: Cerambycidae)","authors":"Nan Song, Hao Zhang, Xinming Yin, Aili Lin, Qing Zhai","doi":"10.3109/19401736.2015.1122766","DOIUrl":"https://doi.org/10.3109/19401736.2015.1122766","url":null,"abstract":"Abstract The complete mitochondrial genome (mitogenome) of Obrium sp. was sequenced, which was the first representation from the longicorn beetle genus Obrium. The whole mitogenome is a typical circular DNA molecule of 15 680 bp, and has similar gene content and gene order to previously reported beetle species. In the sampled subfamilies of Cerambycidae, both Lamiinae and Cerambycinae were recovered with high supports in phylogenetic analysis. And the newly sequenced Obrium sp. was the sister group to Massicus raddei in Lamiinae.","PeriodicalId":54298,"journal":{"name":"Mitochondrial Dna Part a","volume":"66 1","pages":"326 - 327"},"PeriodicalIF":0.0,"publicationDate":"2017-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83834886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Determining geographical variations in Ascaris suum isolated from different regions in northwest China through sequences of three mitochondrial genes 利用3个线粒体基因序列测定西北不同地区猪蛔虫的地理变异
4区 生物学
Mitochondrial Dna Part a Pub Date : 2017-05-01 DOI: 10.3109/19401736.2015.1129404
Yong Zou, Fei Wu, Ya-Xu Guo, Hui-Bao Wang, Yan-Qin Fang, Ming Kang, Q. Lin
{"title":"Determining geographical variations in Ascaris suum isolated from different regions in northwest China through sequences of three mitochondrial genes","authors":"Yong Zou, Fei Wu, Ya-Xu Guo, Hui-Bao Wang, Yan-Qin Fang, Ming Kang, Q. Lin","doi":"10.3109/19401736.2015.1129404","DOIUrl":"https://doi.org/10.3109/19401736.2015.1129404","url":null,"abstract":"Abstract The sequence diversities in three mitochondrial DNA (mtDNA) regions, namely portions of NADH dehydrogenase subunit 1 (pnad1), cytochrome c oxidase subunit 1 (pcox1), and NADH dehydrogenase subunit 4 (pnad4), were investigated in all Ascaris suum samples isolated from four regions in northwestern China. Those genes were amplified by PCR method and the lengths of pnad1, pcox1, and pnad4 were 419 bp, 711 bp, and 723 bp, respectively. The intraspecific sequence variations within A. suum samples were 0–2.9% for pnad1, 0–2.1% for pcox1, and 0–3.1% for pnad4. Phylogenetic analysis combined with three sequences of mtDNA fragments showed that all A. suum samples were monophyletic groups, but samples from the same geographical origin did not always cluster together. The results suggested that the three mtDNA fragments could not be used as molecular markers to identify the A. suum isolates from four regions, and have important implications for studying molecular epidemiology and population genetics of A. suum.","PeriodicalId":54298,"journal":{"name":"Mitochondrial Dna Part a","volume":"10 1","pages":"411 - 415"},"PeriodicalIF":0.0,"publicationDate":"2017-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78402919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Complete mitochondrial genome of Hepialus xiaojinensis (Lepidoptera: Hepialidae) 小金河鼠线粒体全基因组(鳞翅目:河鼠科)
4区 生物学
Mitochondrial Dna Part a Pub Date : 2017-05-01 DOI: 10.3109/19401736.2015.1118089
Shijiang Chen, Ping Shi, De-li Zhang, Zeng-hui Lu, L. Li
{"title":"Complete mitochondrial genome of Hepialus xiaojinensis (Lepidoptera: Hepialidae)","authors":"Shijiang Chen, Ping Shi, De-li Zhang, Zeng-hui Lu, L. Li","doi":"10.3109/19401736.2015.1118089","DOIUrl":"https://doi.org/10.3109/19401736.2015.1118089","url":null,"abstract":"Abstract Although Hepialus xiaojinensis is important as the host of Ophiocordyceps sinensis, it remains poorly known especially in genetic structure and phylogeny status. To get a better understanding of it, the complete mitochondrial genome sequence of H. xiaojinensis was sequenced for the first time. The genome was 15 397 bp in size and contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and an AT-rich region, and the gene composition and the arrangement of which were consistent with other insects of Hepialidae. The base composition of the genome was A (41.44%), T (39.68%), C (11.52%), and G (7.36%), with an AT content of 81.13%. Moreover, we have performed phylogenetic analysis of H. xiaojinensis and other 11 Lepidoptera species.","PeriodicalId":54298,"journal":{"name":"Mitochondrial Dna Part a","volume":"2 1","pages":"305 - 306"},"PeriodicalIF":0.0,"publicationDate":"2017-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79733086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
The phylogenetic placement of Odontobutis obscura base on complete mitochondrial DNA sequence 基于全线粒体DNA序列的黑齿蟾系统发育定位
4区 生物学
Mitochondrial Dna Part a Pub Date : 2017-05-01 DOI: 10.3109/19401736.2015.1127363
Yandong Niu, Xiquan Li, Canming Zhang, Ying Wei
{"title":"The phylogenetic placement of Odontobutis obscura base on complete mitochondrial DNA sequence","authors":"Yandong Niu, Xiquan Li, Canming Zhang, Ying Wei","doi":"10.3109/19401736.2015.1127363","DOIUrl":"https://doi.org/10.3109/19401736.2015.1127363","url":null,"abstract":"Abstract In our research, 16 sets of primers were used to amplify contiguous, overlapping segments of the complete mitochondrial DNA (mtDNA) of Odontobutis obscura. The aim of our research was to provide some important genetic background information for conservational practice of these valuable fish species. The total length of the mitochondrial genome is 16 864 bp and deposited in the GenBank with accession numbers KT438552. The gene arrangement and transcriptional direction were similar to other bony fishes which contained 37 genes (13 protein-coding genes, 2 ribosomal RNA, and 22 transfer RNAs) and a major non-coding control region. The nucleotide skewness for the coding strands of O. obscura (GC-skew = −0.26) is biased toward G and the negative GC-skew ranges from −0.51(ND2) to −0.24(CO1). The phylogenetic analysis showed that the Gobiiformes could be divided into three groups, Eleotridae, Odontobutidae and Rhyacichthyidae. The O. Obscura and O. Potamophila are sister species and clustered together.","PeriodicalId":54298,"journal":{"name":"Mitochondrial Dna Part a","volume":"43 1","pages":"399 - 400"},"PeriodicalIF":0.0,"publicationDate":"2017-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80796098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Sequencing and characterization of the Megachile sculpturalis (Hymenoptera: Megachilidae) mitochondrial genome 巨型蜂线粒体基因组的测序和鉴定(膜翅目:巨型蜂科
4区 生物学
Mitochondrial Dna Part a Pub Date : 2017-05-01 DOI: 10.3109/19401736.2015.1122774
Yan Zhang, Tianjuan Su, Bo He, P. Gu, Dunyuan Huang, Chaodong Zhu
{"title":"Sequencing and characterization of the Megachile sculpturalis (Hymenoptera: Megachilidae) mitochondrial genome","authors":"Yan Zhang, Tianjuan Su, Bo He, P. Gu, Dunyuan Huang, Chaodong Zhu","doi":"10.3109/19401736.2015.1122774","DOIUrl":"https://doi.org/10.3109/19401736.2015.1122774","url":null,"abstract":"Abstract The mitochondrial genome (mitogenome) of Megachile sculpturalis (Hymenoptera: Megachilidae) has been reported in this study. This is the first sequenced mitogenome from the family Megachilidae. The sequenced region of this mitochondrial genome is determined to be 16 581 bp in length, including 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs, and a partial sequence of the AT-rich region, and the AT-rich region contains several characteristic repeated sequences. Additionally, we conducted a phylogenetic analysis using all the mitochondrial genomes of Apoidea that are available in Genbank. Bayesian method supported the monophyly of both Apidae and Colletidae. And Megachilidae was inferred as the sister group of Apidae.","PeriodicalId":54298,"journal":{"name":"Mitochondrial Dna Part a","volume":"100 1","pages":"344 - 346"},"PeriodicalIF":0.0,"publicationDate":"2017-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81076794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
The complete mitochondrial genome of Paracheirodon innesi 中华副剑齿虎线粒体全基因组
4区 生物学
Mitochondrial Dna Part a Pub Date : 2017-05-01 DOI: 10.3109/19401736.2015.1126830
Ai-fen Yan, Fang Liu, Hong Jiang, Cuilan Feng, Dong-sheng Tang
{"title":"The complete mitochondrial genome of Paracheirodon innesi","authors":"Ai-fen Yan, Fang Liu, Hong Jiang, Cuilan Feng, Dong-sheng Tang","doi":"10.3109/19401736.2015.1126830","DOIUrl":"https://doi.org/10.3109/19401736.2015.1126830","url":null,"abstract":"Abstract The complete mitochondrial genome of Paracheirodon innesi was determined using the next-generation sequencing (NGS). The mitochondrial genome was 16 962 bp long and consisted of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region. The gene arrangement and composition of P. Innesi mitochondrial genome was similar to that of most other vertebrates. Phylogenetic tree was constructed based on the complete mitogenomes of the species and closely related 13 teleost species to assess their phylogenetic relationship and evolution.","PeriodicalId":54298,"journal":{"name":"Mitochondrial Dna Part a","volume":"9 1","pages":"377 - 378"},"PeriodicalIF":0.0,"publicationDate":"2017-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73770266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
The first taxonomic revaluation of the Iranian water frogs of the genus Pelophylax (Anura: Ranidae) using sequences of the mitochondrial genome 利用线粒体基因组序列对伊朗水蛙属(无尾目:蛙科)进行首次分类重估
4区 生物学
Mitochondrial Dna Part a Pub Date : 2017-05-01 DOI: 10.3109/19401736.2015.1127362
A. Pesarakloo, E. Rastegar-pouyani, N. Rastegar-Pouyani, H. Kami, M. Najibzadeh, A. Khosravani, H. Oraie
{"title":"The first taxonomic revaluation of the Iranian water frogs of the genus Pelophylax (Anura: Ranidae) using sequences of the mitochondrial genome","authors":"A. Pesarakloo, E. Rastegar-pouyani, N. Rastegar-Pouyani, H. Kami, M. Najibzadeh, A. Khosravani, H. Oraie","doi":"10.3109/19401736.2015.1127362","DOIUrl":"https://doi.org/10.3109/19401736.2015.1127362","url":null,"abstract":"Abstract The Eurasian water frog species and their geographic ranges have undergone considerable changes in the last four decades, but the Iranian populations have largely remained unknown. All the Iranian populations of water frogs, despite their vast distribution range have attributed to a single species: Rana ridibunda. In order to understand the phylogenetic relationships and taxonomic status of water frogs of Iran, we collected samples from many populations across the country and used the mitochondrial DNA sequence variation. A data set with a final sequence length of 616 nucleotides was generated for Cyt b from 70 individuals of Pelophylax in which there are 422 invariable sites, 174 variable sites of which 123 were parsimony informative. In total, 43 haplotypes were found (Hd: 0.9752). The result demonstrated that, two major clades with strong support can be identified within the Iranian water frogs. One of these clades that include north western and southwestern populations forms a monophyletic group along with P. bedriagae samples from Turkey. The second clade consists of water frog populations of north and northeastern parts of Iran which in turn is subdivided into two subclades. Inclusion of water frog samples from adjacent areas showed that the second clade of our study is, most likely, a distinct taxonomic entity at species rank with its two subclades indicating two diagnosable subspecies for the clade. In conclusion, we suggest that two distinct species, P. bedriagae and Pelophylax sp., with its two subspecies, should be identified as water frogs of Iran. In Addition, another traditionally reported water frog of Iran, P.ridibundus, most likely should be excluded from the Iranian water frog’s checklist.","PeriodicalId":54298,"journal":{"name":"Mitochondrial Dna Part a","volume":"196 1","pages":"392 - 398"},"PeriodicalIF":0.0,"publicationDate":"2017-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75896369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
The complete chloroplast genome of Tibetan hulless barley 西藏无壳大麦叶绿体全基因组
4区 生物学
Mitochondrial Dna Part a Pub Date : 2017-05-01 DOI: 10.3109/19401736.2015.1122765
Quan Zeng, J. H. Yuan, L. Wang, Jun Qi Xu, T. Nyima
{"title":"The complete chloroplast genome of Tibetan hulless barley","authors":"Quan Zeng, J. H. Yuan, L. Wang, Jun Qi Xu, T. Nyima","doi":"10.3109/19401736.2015.1122765","DOIUrl":"https://doi.org/10.3109/19401736.2015.1122765","url":null,"abstract":"Abstract We report complete chloroplast genome of the Tibetan hulless barely using IIlumina Hiseq 2000 sequencing technology (IIlumina Inc., San Diego, CA). The chloroplast genome size is 136 462 bp in length that includes two inverted repeats (IRs) of 10 528 bp, which are separated by the large single copy (LSC 101 375 bp) and small single copy (SSC 14 030 bp). Hulless barely chloroplast genome encodes 76 protein-coding genes, four rRNA genes, and 29 tRNA genes. The maximum-likelihood (ML) phylogenetic tree of the nine complete chloroplast genomes was selected from Poaceae family using Oryza sativa japonica as the out-group, supporting that hulless barely is closely related to the Hordeum vulgare subsp. vulgare.","PeriodicalId":54298,"journal":{"name":"Mitochondrial Dna Part a","volume":"427 1","pages":"324 - 325"},"PeriodicalIF":0.0,"publicationDate":"2017-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84955009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
The complete mitochondrial genome sequence of Buteo hemilasius (Falconiformes: Accipitridae) 半爪蟾线粒体全基因组序列(隼形目:棘足蟾科)
4区 生物学
Mitochondrial Dna Part a Pub Date : 2017-05-01 DOI: 10.3109/19401736.2015.1122764
Guolei Sun, Xiufeng Yang, Yongqiang Lu, Xiaodong Gao, Chao-Yi Zhao, H. Dou, Honghai Zhang
{"title":"The complete mitochondrial genome sequence of Buteo hemilasius (Falconiformes: Accipitridae)","authors":"Guolei Sun, Xiufeng Yang, Yongqiang Lu, Xiaodong Gao, Chao-Yi Zhao, H. Dou, Honghai Zhang","doi":"10.3109/19401736.2015.1122764","DOIUrl":"https://doi.org/10.3109/19401736.2015.1122764","url":null,"abstract":"Abstract In this study, the complete mitochondrial genome of the Upland Buzzard (Buteo hemilasius) was sequenced and reported for the first time using the muscle tissue. The mitochondrial genome is a circular molecule of 18 079 bp in length, consisting of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, 1 control region, and 1 extra pseudo-control region. A phylogenetic analysis on the basis of 12 protein-coding genes (except ND6 gene) of 14 Falconiformes species’ mitochondrial genomes using maximum-likelihood (ML) and Bayesian inference (BI) demonstrated that all species cluster into two clades and B. hemilasius is close to B. buteo.","PeriodicalId":54298,"journal":{"name":"Mitochondrial Dna Part a","volume":"26 1","pages":"322 - 323"},"PeriodicalIF":0.0,"publicationDate":"2017-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84823799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The complete mitochondrial genome of the Placidochromis longimanus 长尾长尾龙的线粒体全基因组
4区 生物学
Mitochondrial Dna Part a Pub Date : 2017-05-01 DOI: 10.3109/19401736.2015.1122772
Y. Xiong, Yuxia Qin, Liyun Liu, Jing Liu, Jie Jiang, Pei Liu, Lianqin Zhang, Dashi Qi, Zongyun Li
{"title":"The complete mitochondrial genome of the Placidochromis longimanus","authors":"Y. Xiong, Yuxia Qin, Liyun Liu, Jing Liu, Jie Jiang, Pei Liu, Lianqin Zhang, Dashi Qi, Zongyun Li","doi":"10.3109/19401736.2015.1122772","DOIUrl":"https://doi.org/10.3109/19401736.2015.1122772","url":null,"abstract":"Abstract The Placidochromis longimanus (P. longimanus), one species of Cichlidae, resides in the Lake Malawi in East Africa. In present study, for the first time, we reported the complete mitochondrial genome of P. longimanus, which has 16 581 bp in length, including 13 protein-coding genes, 2 ribosomal RNA, 22 transfer RNA genes, and 1 control zone. Moreover, its GC content is 45.99% (27.44% A, 26.58% T, 30.11% C, and 15.87% G), similar to that of Astatotilapia calliptera (the GC content of 45.90%). We further made the phylogenetic tree on the complete mitochondrial genomes of the above two species and other 11 closely related species to show their phylogenic relationship. The above results would facilitate our understanding of the evolution of Cichlidae mitochondrial genome.","PeriodicalId":54298,"journal":{"name":"Mitochondrial Dna Part a","volume":"24 1","pages":"340 - 341"},"PeriodicalIF":0.0,"publicationDate":"2017-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73742408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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