利用3个线粒体基因序列测定西北不同地区猪蛔虫的地理变异

IF 1.1 4区生物学 Q4 GENETICS & HEREDITY

Mitochondrial Dna Part a Pub Date : 2017-05-01 DOI:10.3109/19401736.2015.1129404

Yong Zou, Fei Wu, Ya-Xu Guo, Hui-Bao Wang, Yan-Qin Fang, Ming Kang, Q. Lin

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引用次数: 3

摘要

摘要研究了中国西北4个地区的猪蛔虫线粒体DNA (mtDNA) 3个区域，即NADH脱氢酶亚基1 (pnad1)、细胞色素c氧化酶亚基1 (pcox1)和NADH脱氢酶亚基4 (pnad4)部分序列的多样性。PCR扩增得到pnad1、pcox1和pnad4基因长度分别为419 bp、711 bp和723 bp。pnad1、pcox1和pnad4的种内序列变异分别为0-2.9%、0-2.1%和0-3.1%。结合3个mtDNA片段序列的系统发育分析表明，所有标本均为单系类群，但来自同一地理来源的标本并不总是聚集在一起。结果表明，这3个mtDNA片段不能作为分子标记来鉴别4个地区的猪猪分离株，对研究猪猪的分子流行病学和群体遗传学具有重要意义。

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Determining geographical variations in Ascaris suum isolated from different regions in northwest China through sequences of three mitochondrial genes

Abstract The sequence diversities in three mitochondrial DNA (mtDNA) regions, namely portions of NADH dehydrogenase subunit 1 (pnad1), cytochrome c oxidase subunit 1 (pcox1), and NADH dehydrogenase subunit 4 (pnad4), were investigated in all Ascaris suum samples isolated from four regions in northwestern China. Those genes were amplified by PCR method and the lengths of pnad1, pcox1, and pnad4 were 419 bp, 711 bp, and 723 bp, respectively. The intraspecific sequence variations within A. suum samples were 0–2.9% for pnad1, 0–2.1% for pcox1, and 0–3.1% for pnad4. Phylogenetic analysis combined with three sequences of mtDNA fragments showed that all A. suum samples were monophyletic groups, but samples from the same geographical origin did not always cluster together. The results suggested that the three mtDNA fragments could not be used as molecular markers to identify the A. suum isolates from four regions, and have important implications for studying molecular epidemiology and population genetics of A. suum.

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来源期刊

Mitochondrial Dna Part a Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： Mitochondrial DNA Part A publishes original high-quality manuscripts on physical, chemical, and biochemical aspects of mtDNA and proteins involved in mtDNA metabolism, and/or interactions. Manuscripts on cytosolic and extracellular mtDNA, and on dysfunction caused by alterations in mtDNA integrity as well as methodological papers detailing novel approaches for mtDNA manipulation in vitro and in vivo are welcome. Descriptive papers on DNA sequences from mitochondrial genomes, and also analytical papers in the areas of population genetics, phylogenetics and human evolution that use mitochondrial DNA as a source of evidence for studies will be considered for publication. The Journal also considers manuscripts that examine population genetic and systematic theory that specifically address the use of mitochondrial DNA sequences, as well as papers that discuss the utility of mitochondrial DNA information in medical studies and in human evolutionary biology.