Mitochondrial Dna Part a最新文献_第9页

Mitochondrial DNA phylogeny of camel spiders (Arachnida: Solifugae) from Iran 伊朗骆驼蛛(蛛形纲:骆驼蛛科)线粒体DNA系统发育

4区生物学

Mitochondrial Dna Part a Pub Date : 2017-11-01 DOI: 10.1080/24701394.2016.1209194

Hassan Maddahi, Mahsa Khazanehdari, M. Aliabadian, H. Kami, A. Mirshamsi, O. Mirshamsi

{"title":"Mitochondrial DNA phylogeny of camel spiders (Arachnida: Solifugae) from Iran","authors":"Hassan Maddahi, Mahsa Khazanehdari, M. Aliabadian, H. Kami, A. Mirshamsi, O. Mirshamsi","doi":"10.1080/24701394.2016.1209194","DOIUrl":"https://doi.org/10.1080/24701394.2016.1209194","url":null,"abstract":"Abstract In the present study, the mitochondrial DNA phylogeny of five solifuge families of Iran is presented using phylogenetic analysis of mitochondrial cytochrome c oxidase, subunit 1 (COI) sequence data. Moreover, we included available representatives from seven families from GenBank to examine the genetic distance between Old and New World taxa and test the phylogenetic relationships among more solifuge families. Phylogenetic relationships were reconstructed based on the two most probabilistic methods, Maximum Likelihood (ML) and Bayesian inference (BI) approaches. Resulting topologies demonstrated the monophyly of the families Daesiidae, Eremobatidae, Galeodidae, Karschiidae and Rhagodidae, whereas the monophyly of the families Ammotrechidae and Gylippidae was not supported. Also, within the family Eremobatidae, the subfamilies Eremobatinae and Therobatinae and the genus Hemerotrecha were paraphyletic or polyphyletic. According to the resulted topologies, the taxonomic placements of Trichotoma michaelseni (Gylippidae) and Nothopuga sp. 1 (Ammotrechidae) are still remain under question and their revision might be appropriate. According to the results of this study, within the family Galeodidae, the validity of the genus Galeodopsis is supported, while the validity of the genus Paragaleodes still remains uncertain. Moreover, our results revealed that the species Galeodes bacillatus, and Rhagodes melanochaetus are junior synonyms of G. caspius, and R. eylandti, respectively.","PeriodicalId":54298,"journal":{"name":"Mitochondrial Dna Part a","volume":"69 1","pages":"909 - 919"},"PeriodicalIF":0.0,"publicationDate":"2017-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84346630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Identification of fraud (with pig stuffs) in chicken-processed meat through information of mitochondrial cytochrome b 利用线粒体细胞色素b的信息识别鸡肉加工中的假猪肉

4区生物学

Mitochondrial Dna Part a Pub Date : 2017-11-01 DOI: 10.1080/24701394.2016.1197220

H. Yacoub, M. A. Sadek

{"title":"Identification of fraud (with pig stuffs) in chicken-processed meat through information of mitochondrial cytochrome b","authors":"H. Yacoub, M. A. Sadek","doi":"10.1080/24701394.2016.1197220","DOIUrl":"https://doi.org/10.1080/24701394.2016.1197220","url":null,"abstract":"Abstract This study was conducted to find out the fraud in chicken-processed meat ingredients to protect consumers from commercial adulteration and authentication through a reliable way: direct amplification of conserved segment of cytochrome b gene of mitochondrial DNA, in addition, using species-specific primer assay for a certain cytochrome b. The results reported that chicken-processed meats were identified as a chicken meat based on amplification of conserved cytochrome b gene of mtDNA, while different fragments sizes were produced after the application of species-specific primer as follows: 227, 157, 274, 331, 389 and 439 bp for raw meat of chicken, goat, cattle, sheep, pig and horse, respectively. The results revealed that all chicken meat products are produced with 227 bp in size. While, an adulteration with pork stuffs was observed in some of the chicken meat products using a species-specific primer of cytochrome b gene, namely, chicken luncheon and chicken burger. This study represents a reliable technique that could be used to provide a promising solution for identifying the commercial adulteration and substitutions in processed meat in retail markets.","PeriodicalId":54298,"journal":{"name":"Mitochondrial Dna Part a","volume":"28 1","pages":"855 - 859"},"PeriodicalIF":0.0,"publicationDate":"2017-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83520416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Unexpected diversity of sandflies (Diptera: Psychodidae) in tourist caves in Northern Thailand 泰国北部旅游洞穴中出人意料的白蛉多样性(双翅目:白蛉科)

4区生物学

Mitochondrial Dna Part a Pub Date : 2017-11-01 DOI: 10.1080/24701394.2016.1214728

Jedsada Sukantamala, K. Sing, N. Jaturas, R. Polseela, John-James Wilson

{"title":"Unexpected diversity of sandflies (Diptera: Psychodidae) in tourist caves in Northern Thailand","authors":"Jedsada Sukantamala, K. Sing, N. Jaturas, R. Polseela, John-James Wilson","doi":"10.1080/24701394.2016.1214728","DOIUrl":"https://doi.org/10.1080/24701394.2016.1214728","url":null,"abstract":"Abstract Certain species of Phlebotomine sandflies (Diptera: Psychodidae) are vectors of the protozoa which causes leishmaniasis. Sandflies are found breeding in enclosed places like caves. Thailand is a popular tourist destination, including for ecotourism activities like caving, which increases the risk of contact between tourists and sandflies. Surveillance of sandflies is important for monitoring this risk but identification of species based on morphology is challenged by phenotypic plasticity and cryptic diversity. DNA barcodes have been used for the identification of sandflies in Thailand. We collected sandflies using CDC light trap from four tourist caves in Northern Thailand. Female sandflies were provisionally sorted into 13 morphospecies and 19 unidentified specimens. DNA was extracted from the thorax and legs of sandflies and the DNA barcode region of cytochrome c oxidase I mtDNA amplified and sequenced. The specimens were sorted into 22 molecular operational taxonomic units (MOTU) based on the 145 DNA barcodes, which is significantly more than the morphospecies. Several of the taxa thought to be present in multiple caves, based on morphospecies sorting, split into cave-specific MOTU which likely represent cryptic species. Several MOTU reported in an earlier study from Wihan Cave, Thailand, were also found in these caves. This supports the use of DNA barcodes to investigate species diversity of sandflies and their useful role in surveillance of sandflies in Thailand.","PeriodicalId":54298,"journal":{"name":"Mitochondrial Dna Part a","volume":"54 1","pages":"949 - 955"},"PeriodicalIF":0.0,"publicationDate":"2017-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84265566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Massive parallel sequencing of human whole mitochondrial genomes with Ion Torrent technology: an optimized workflow for Anthropological and Population Genetics studies 用离子激流技术对人类全线粒体基因组进行大规模平行测序:人类学和种群遗传学研究的优化工作流程

4区生物学

Mitochondrial Dna Part a Pub Date : 2017-11-01 DOI: 10.1080/24701394.2016.1197218

S. De Fanti, D. Vianello, C. Giuliani, Andrea Quagliariello, Anna Cherubini, F. Sevini, Nicoletta Iaquilano, C. Franceschi, M. Sazzini, D. Luiselli

{"title":"Massive parallel sequencing of human whole mitochondrial genomes with Ion Torrent technology: an optimized workflow for Anthropological and Population Genetics studies","authors":"S. De Fanti, D. Vianello, C. Giuliani, Andrea Quagliariello, Anna Cherubini, F. Sevini, Nicoletta Iaquilano, C. Franceschi, M. Sazzini, D. Luiselli","doi":"10.1080/24701394.2016.1197218","DOIUrl":"https://doi.org/10.1080/24701394.2016.1197218","url":null,"abstract":"Abstract Investigation of human mitochondrial DNA variation patterns and phylogeny has been extensively used in Anthropological and Population Genetics studies and sequencing the whole mitochondrial genome is progressively becoming the gold standard. Among the currently available massive parallel sequencing technologies, Ion Torrent™ semiconductor sequencing represents a promising approach for such studies. Nevertheless, an experimental protocol conceived to enable the achievement of both as high as possible yield and of the most homogeneous sequence coverage through the whole mitochondrial genome is still not available. The present work was thus aimed at improving the overall performance of whole mitochondrial genomes Ion Torrent™ sequencing, with special focus on the capability to obtain robust coverage and highly reliable variants calling. For this purpose, a series of cost-effective modifications in standard laboratory workflows was fine-tuned to optimize them for medium- and large-scale population studies. A total of 54 human samples were thus subjected to sequencing of the whole mitochondrial genome with the Ion Personal Genome Machine™ System in four distinct experiments and using Ion 314 chips. Seven of the selected samples were also characterized by means of conventional Sanger sequencing for the sake of comparison. Obtained results demonstrated that the implemented optimizations had definitely improved sequencing outputs in terms of both variants calling efficiency and coverage uniformity, enabling to setup an effective and accurate protocol for whole mitochondrial genome sequencing and a considerable reduction in experimental time consumption and sequencing costs.","PeriodicalId":54298,"journal":{"name":"Mitochondrial Dna Part a","volume":"34 1","pages":"843 - 850"},"PeriodicalIF":0.0,"publicationDate":"2017-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90018222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Forensically informative nucleotide sequencing (FINS) for the first time authentication of Indian Varanus species: implication in wildlife forensics and conservation 首次鉴定印度Varanus物种的法医信息核苷酸测序(FINS):在野生动物法医和保护中的意义

4区生物学

Mitochondrial Dna Part a Pub Date : 2017-11-01 DOI: 10.1080/24701394.2016.1202943

Ankita Rajpoot, V. Kumar, A. Bahuguna, Dhyanendra Kumar

{"title":"Forensically informative nucleotide sequencing (FINS) for the first time authentication of Indian Varanus species: implication in wildlife forensics and conservation","authors":"Ankita Rajpoot, V. Kumar, A. Bahuguna, Dhyanendra Kumar","doi":"10.1080/24701394.2016.1202943","DOIUrl":"https://doi.org/10.1080/24701394.2016.1202943","url":null,"abstract":"Abstract Monitor lizards are Varanus species widely distributed, endangered reptile in the IUCN red data list. In India, based on the morphological and ecological characteristic, it is divided into four species viz. Bengal monitor lizard, Yellow monitor lizard, Desert monitor lizard and Water monitor lizard. These four species listed as Schedule I species in Indian Wildlife (Protection) Act 1972. This paper first attempt to present Forensically Informative Nucleotide Sequencing (FINS) for the Indian Varanus based on three mitochondrial genes. The molecular framework will be useful for the identification of Indian Varanus species and trade products derived from monitors and as such, have important applications for wildlife management and conservation. Here, we used known 14 individual skin pieces of four species of monitor lizards; the partial fragment of three mitochondrial genes (Cyt b, 12S rRNA, and 16S rRNA) were amplified for genetic study. In Cyt b, 12S rRNA and 16s rRNA, we observed, 5, 5 and 4 Haplotypes; 71, 69, and 43 Variables sites; 90, 89, and 50 Parsimony Informative sites within four species of Indian monitor lizards, respectively. Despite it, the nucleotide composition was T 26.4, C 32.8, A 29.2 and G11.6; T 18.8, C 29.7, A 34.0 and G 17.5; T 21.7, C 27.3, A 32.5 and G 18.5 in Cyt b, 12S rRNA and 16S rRNA, respectively. The neighbor joining phylogenetic tree and maximum parsimony tree of three mitochondrial genes, showed similar results and reveal that, there are two major clades are present in Indian monitor lizards.","PeriodicalId":54298,"journal":{"name":"Mitochondrial Dna Part a","volume":"1 1","pages":"892 - 900"},"PeriodicalIF":0.0,"publicationDate":"2017-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83095141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

High occurrence of length heteroplasmy in domestic Bactrian camel (Camelus bactrianus) 国内双峰驼(Camelus bactrianus)体长异质性发生率高

4区生物学

Mitochondrial Dna Part a Pub Date : 2017-11-01 DOI: 10.1080/24701394.2016.1197219

Xiao-hong He, Xiaofei Chen, Wenbin Zhang, Y. Pu, Shen Song, Jianlin Han, K. Dong, Qianjun Zhao, W. Guan, Yuehui Ma, Lin Jiang

引用次数: 3

Detecting signatures of selection within the Tibetan sheep mitochondrial genome 检测藏羊线粒体基因组的选择特征

4区生物学

Mitochondrial Dna Part a Pub Date : 2017-11-01 DOI: 10.1080/24701394.2016.1192614

L. Niu, Xiaoyong Chen, Ping Xiao, Qianjun Zhao, Jingxuan Zhou, Jiangtao Hu, Hongxin Sun, Jiazhong Guo, Li Li, Linjie Wang, Hongping Zhang, T. Zhong

{"title":"Detecting signatures of selection within the Tibetan sheep mitochondrial genome","authors":"L. Niu, Xiaoyong Chen, Ping Xiao, Qianjun Zhao, Jingxuan Zhou, Jiangtao Hu, Hongxin Sun, Jiazhong Guo, Li Li, Linjie Wang, Hongping Zhang, T. Zhong","doi":"10.1080/24701394.2016.1192614","DOIUrl":"https://doi.org/10.1080/24701394.2016.1192614","url":null,"abstract":"Abstract Tibetan sheep, a Chinese indigenous breed, are mainly distributed in plateau and mountain-valley areas at a terrestrial elevation between 2260 and 4100 m. The herd is genetically distinct from the other domestic sheep and undergoes acclimatization to adapt to the hypoxic environment. To date, whether the mitochondrial DNA modification of Tibetan sheep shares the same feature as the other domestic breed remains unknown. In this study, we compared the whole mitogenome sequences from 32 Tibetan sheep, 22 domestic sheep and 24 commercial sheep to identify the selection signatures of hypoxic-tolerant in Tibetan sheep. Nucleotide diversity analysis using the sliding window method showed that the highest level of nucleotide diversity was observed in the control region with a peak value of π = 0.05215, while the lowest π value was detected in the tRNAs region. qPCR results showed that the relative mtDNA copy number in Tibetan sheep was significantly lower than that in Suffolk sheep. None of the mutations in 12S rRNA were fixed in Tibetan sheep, which indicated that there has been less artificial selection in this herd than the other domestic and commercial breeds. Although one site (1277G) might undergo the purifying selection, it was not identified as the breed-specific allele in Tibetan sheep. We proposed that nature selection was the main drive during the domestication of Tibetan sheep and single mutation (or locus) could not reveal the signature of selection as for the high diversity in the mitogenome of Tibetan sheep.","PeriodicalId":54298,"journal":{"name":"Mitochondrial Dna Part a","volume":"31 1","pages":"801 - 809"},"PeriodicalIF":0.0,"publicationDate":"2017-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91205651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Genetic characterization of wild swamp deer populations: ex situ conservation and forensics implications 野生沼泽鹿种群的遗传特征:迁地保护和法医学意义

4区生物学

Mitochondrial Dna Part a Pub Date : 2017-11-01 DOI: 10.1080/24701394.2016.1225732

V. Kumar, A. Shrivastwa, P. Nigam, Dhyanendra Kumar, S. Goyal

{"title":"Genetic characterization of wild swamp deer populations: ex situ conservation and forensics implications","authors":"V. Kumar, A. Shrivastwa, P. Nigam, Dhyanendra Kumar, S. Goyal","doi":"10.1080/24701394.2016.1225732","DOIUrl":"https://doi.org/10.1080/24701394.2016.1225732","url":null,"abstract":"Abstract Swamp deer (Rucervus duvaucelii) is an endemic, Scheduled I species under the Wildlife (Protection) Act 1972, India. According to variations in antler size, it has been classified into three subspecies, namely Western (R. duvaucelii duvaucelii), Central (R. duvaucelii branderi), and Eastern (R. duvaucelii ranjitsinhii). For planning effective ex situ and in situ conservation of a wide-ranging species in different bioclimatic regions and in wildlife forensic, the use of genetic characterization in defining morpho/ecotypes has been suggested because of the geographic clines and reproductive isolation. In spite of these morphotypes, very little is known about the genetic characteristics of the three subspecies, hence no strict subspecies-based breeding plan for retaining the evolutionary characteristics in captive populations for subsequent re-introduction is available except for a few studies. We describe the genetic characteristics of these three subspecies using cytochrome b of the mtDNA genome (400 bp). The DNA sequence data indicated 11 variable sites within the three subspecies. Two paraphyletic clades, namely the Central India and Western-Eastern populations were found, whereas the Western and Eastern populations are monophyletic with a bootstrap value of 69% within the clade. We suggest the need of sorting these three subspecies using different molecular mtDNA markers in zoos for captive breeding purposes so as to retain the genetic diversity of the separate geographic clines and to use a subspecies-specific fixed-state nucleotide to assess the extent of poaching to avoid any population demography stochastically in India.","PeriodicalId":54298,"journal":{"name":"Mitochondrial Dna Part a","volume":"34 1","pages":"965 - 970"},"PeriodicalIF":0.0,"publicationDate":"2017-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85442965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Phylogeography of the endangered orchid Dendrobium moniliforme in East Asia inferred from chloroplast DNA sequences 从叶绿体DNA序列推断东亚濒危兰花石斛的系统地理学

4区生物学

Mitochondrial Dna Part a Pub Date : 2017-11-01 DOI: 10.1080/24701394.2016.1202942

Meirong Ye, Wei Liu, Qing-yun Xue, B. Hou, Jing Luo, Xiaoyu Ding

{"title":"Phylogeography of the endangered orchid Dendrobium moniliforme in East Asia inferred from chloroplast DNA sequences","authors":"Meirong Ye, Wei Liu, Qing-yun Xue, B. Hou, Jing Luo, Xiaoyu Ding","doi":"10.1080/24701394.2016.1202942","DOIUrl":"https://doi.org/10.1080/24701394.2016.1202942","url":null,"abstract":"Abstract The aim of the current study was to elucidate the phylogeographic history of Dendrobium moniliforme, an endangered orchid species, based on two chloroplast DNA (cpDNA) markers (trnC-petN and trnE-trnT). One hundred and thirty-five samples were collected from 18 natural populations of D. moniliforme covering the entire range of the Sino-Japanese Floristic Region (SJFR) of East Asia. A total of 35 distinct cpDNA haplotypes were identified in these populations, of which 23 haplotypes were each present in only one sample and thus restricted to a single population. The significantly larger NST value (0.586) than GST (0.328) (p < 0.05) demonstrated the presence of strong phylogeographic structure. Phylogenetic analyses indicated that all haplotypes were clustered into two lineages. The genetic diversity of D. moniliforme was high at the species level, reflected in its haplotype diversity (Hd=0.8862), nucleotide diversity (Pi=0.00361), total genetic diversity (HT=0.9011), and significant differentiation (ΦST=0.5482). Based on mismatch distribution analysis and neutrality tests, population expansion was evident in all sampled populations and also in all populations sampled in mainland China. Three refuge areas were identified, one each in southwestern China, central-southeastern China, and the CKJ (Taiwan, Japan and Korea) Islands. The results supported the hypothesis that glacial refugia were maintained on different spatial-temporal scales in the SJFR during the last glacial maximum or earlier cold periods, suggesting that Quaternary refugial isolation promoted allopatric speciation of D. moniliforme in East Asia.","PeriodicalId":54298,"journal":{"name":"Mitochondrial Dna Part a","volume":"31 1","pages":"880 - 891"},"PeriodicalIF":0.0,"publicationDate":"2017-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74191420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

An evaluation of the suitability of COI and COII gene variation for reconstructing the phylogeny of, and identifying cryptic species in, anopheline mosquitoes (Diptera Culicidae) COI和COII基因变异对库蚊系统发育重建和隐种鉴定的适用性评价

4区生物学

Mitochondrial Dna Part a Pub Date : 2017-09-01 DOI: 10.1080/24701394.2016.1186665

Gang Wang, Chun-Xiao Li, Wei Zheng, F. Song, Xiao-xia Guo, Zhonghua Wu, Peng Luo, Yongyao Yang, Lei He, T. Zhao

{"title":"An evaluation of the suitability of COI and COII gene variation for reconstructing the phylogeny of, and identifying cryptic species in, anopheline mosquitoes (Diptera Culicidae)","authors":"Gang Wang, Chun-Xiao Li, Wei Zheng, F. Song, Xiao-xia Guo, Zhonghua Wu, Peng Luo, Yongyao Yang, Lei He, T. Zhao","doi":"10.1080/24701394.2016.1186665","DOIUrl":"https://doi.org/10.1080/24701394.2016.1186665","url":null,"abstract":"Abstract We assessed the practicality and effectiveness of using variation in the mitochondrial COI and COII genes to discriminate species and reconstruct the phylogeny of anophelene mosquitoes. Phylogenetic relationships among the subfamily Anophelinae were inferred from portions of the mitochondrial COI (92 species) and COII genes (108 species). Phylogenetic trees were reconstructed on the basis of parsimony, maximum likelihood and Bayesian methods. The suitability of COI and COII gene variation for identifying cryptic species was compared by comparing the sequence divergence within species groups and complexes. The results show that the COI gene was more useful for identifying sibling and cryptic species, but that phylogenetic relationships reconstructed using the COII gene were more similar to those based on morphological data. We conclude that: (1) there is a significant molecular divergence among An. sinensis; (2) the COI and COII are valid genetic markers for resolving taxonomic relationships among anopheline mosquitoes and the resultant phylogeny raises some questions about the taxonomic status of anopheline species groups and complexes; (3) the genus Anopheles is not demonstrably monophyletic with regard to the genus Bironella; (4) the subgenera Kerteszia and Nyssorhynchus are monophyletic; (5) below the group-level, COI data support the existence of monophyletic taxa within the Anopheles funestus, Anopheles maculipennis and Anopheles strode and Anopheles barbirostris subgroups, and within the Anopheles nuneztovari complex, whereas COII data support the monophyletic taxa within the Anopheles minimus and Anopheles oswaldoi subgroups, and Anopheles hyrcanus group. The monophyletic taxa within the Anopheles gambiae and Anopheles albitarsis complexes are supported by both COI and COII data.","PeriodicalId":54298,"journal":{"name":"Mitochondrial Dna Part a","volume":"15 1","pages":"769 - 777"},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85154573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19