{"title":"Enhancing salinity stress tolerance and phenylalanine ammonia lyase gene activity using osmolytes in Moringa seedling production","authors":"Amira R. Osman , Hany M. El-Naggar","doi":"10.1016/j.aoas.2022.06.002","DOIUrl":"10.1016/j.aoas.2022.06.002","url":null,"abstract":"<div><p>The salinity of irrigation water is one of the major abiotic stresses that have a variable reaction to soil structure, which has detrimental impacts on seedling development and quality. The osmolytes can help lessen these adverse effects on the establishment of <em>Moringa oleifera</em> seedlings. Therefore, this study was conducted in Egypt (30″53′30, 87″ N and 30″41′29, 77″ E) using polyethylene bags during May – July 2020 and 2021. Three factors in a factorial split –split plot experiment were arranged in randomized complete block design. The main plots were soil structure; sand:clay 2:1 (V/V) and sand 100 %, whereas the subplot treatments were salinity levels; tap water as a control 0.5, 1.5, 4, and 6 dS m<sup>−1</sup>, and sub–subplot osmolytes; tap water as a control, and glycine betaine, mannitol, proline, and sorbitol all at 0.2 g L<sup>−1</sup> as a soil drench. This study aimed to evaluate the effects of osmolytes and soil structure on growth parameters and phenylalanine ammonia lyase (PAL) gene expression in <em>Moringa</em> seedlings. The study concluded that <em>Moringa</em> seedlings in sand:clay performed significantly (<em>P</em> ˂ 0.05) better than those in the sand. With increasing salinity levels, osmolytes enhanced all seedling growth parameters and PAL gene expression compared with the control in the two soil structures. The order of tolerance to salinity stress was sorbitol ˃ proline ˃ mannitol ˃ glycine betaine ˃ control. <em>Moringa</em> seedlings produced in sand:clay treated with 0.2 g L<sup>−1</sup> sorbitol and salinity at 4 or 6 dS m<sup>−1</sup> were superior in most growth parameters and PAL gene expression.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"67 1","pages":"Pages 127-135"},"PeriodicalIF":5.9,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0570178322000112/pdfft?md5=128b138d7675f577f0a39c73c5732ab2&pid=1-s2.0-S0570178322000112-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44607118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of IAA and BAP application in varying concentration on seed yield and oil quality of Guizotia abyssinica (L.f.) Cass","authors":"Monaswita Talukdar, Dillip Kumar Swain, Pratap Bhanu Singh Bhadoria","doi":"10.1016/j.aoas.2022.02.002","DOIUrl":"10.1016/j.aoas.2022.02.002","url":null,"abstract":"<div><p>A low production of oil seed crops, paired with their increased demand, has become a challenge for the edible oil industry in India. <em>Guizotia abyssinica</em> (L.f.) Cass. (niger seed plant) is an annual, dicotyledonous, multipurpose oil seed crop belonging to the family of Asteraceae. Although this plant produces abundant flowers, it fails to produce mature seeds with vigor due to poor vascularization. We investigated the effects of the plant growth-promoting hormone auxin, indole-3-acetic acid (IAA), and the cytokinin, 6-benzyl aminopurine (BAP) individually and together on the growth and oil quality of niger in lateritic soil. Pot experiments were conducted during the wet seasons of 2014 and 2015 in subtropical eastern India. The plants were sprayed with 25, 50, 75, and 100 mg L<sup>−1</sup> each of IAA and BAP, 50, 75, and 100 mg L<sup>−1</sup> of IAA + BAP, or water (control). When applied individually—IAA or BAP at 75 mg L<sup>−1</sup> (I<sub>75</sub>B<sub>0</sub> or I<sub>0</sub>B<sub>75</sub>)—the biomass production was maximized. The combination of IAA (50 mg L<sup>−1</sup>) and BAP (100 mg L<sup>−1</sup>; I<sub>50</sub>B<sub>100</sub>) yielded significantly high biomass (38 and 40 g plant<sup>−1</sup>) and seed yield (13.24 and 12.67 g plant<sup>−1</sup>) in 2014 and 2015, respectively. The most effective combinations for increasing niger seed oleic acid content were I<sub>50</sub>B<sub>100</sub> and I<sub>50</sub>B<sub>75</sub> as compared to other combinations of growth hormone. These results suggest that exogenous phytohormones can improve the seed yield and oil quality of niger seed plants in acid lateritic soil.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"67 1","pages":"Pages 15-23"},"PeriodicalIF":5.9,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0570178322000021/pdfft?md5=40813bcd0e8ea19cbfaa0a7a1b086a3c&pid=1-s2.0-S0570178322000021-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42625236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B.B.M. Salim , A. Abou El-Yazied , Y.A.M. Salama , Ali Raza , Hany S. Osman
{"title":"Impact of silicon foliar application in enhancing antioxidants, growth, flowering and yield of squash plants under deficit irrigation condition","authors":"B.B.M. Salim , A. Abou El-Yazied , Y.A.M. Salama , Ali Raza , Hany S. Osman","doi":"10.1016/j.aoas.2021.12.003","DOIUrl":"10.1016/j.aoas.2021.12.003","url":null,"abstract":"<div><p>Plant production under climate changes requires unique treatments to overcome the detrimental effects of abiotic stress, such as water deficiency stress. Silicon (Si) has many beneficial effects, especially in plants subjected to different types of stress. Hence, Si foliar application was used to study its potential effects on boosting osmolytes content, activity of antioxidant enzymes, growth of vegetative and flowering organs, and yield of squash plants (<em>Cucurbita pepo</em> L.) under deficit irrigation. Two field experiments were conducted during the winter seasons of 2019 and 2020 at the Experimental Farm, Faculty of Agriculture, Ain Shams University, Egypt. Three concentrations of Si at 0, 2000 and 4000 ppm in the form of monosilicic acid (H<sub>4</sub>SiO<sub>4</sub>) under two levels of water irrigation at 80% of water holding capacity (WHC) as control and 50% of WHC as drought treatment. Drought treatment significantly reduced the growth parameters: shoot height, shoot fresh and dry weights, leaves number/plant, average leaf area, fruit setting and yield traits, and the physiological attributes: leaf relative water content (LRWC), total chlorophylls (SPAD), total soluble proteins (TSP) and catalase (CAT) activity. Meanwhile, significant increases in the concentrations of stress indicators (total free amino acids and proline) were observed compared to the control plants (80% WHC). Both rates of Si achieved marked increases in the vegetative growth, flowering, chlorophylls, LRWC, TSP, superoxide dismutase (SOD), CAT, peroxidase (POD) and polyphenol oxidase (PPO) activity, which in turn reflects on improving fruit setting %, total fruits, and yield of squash plants under well or deficit irrigation. Spraying the high level of Si (4000 ppm) was the most effective treatment that emended the adverse effects of drought.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"66 2","pages":"Pages 176-183"},"PeriodicalIF":5.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0570178321000427/pdfft?md5=0c78465c4ac0bed8cfd29b6db2c539e3&pid=1-s2.0-S0570178321000427-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42001533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An investigation of upland soil fertility from different soil types","authors":"Quoc Thinh Tran, Kiwako S. Araki, Motoki Kubo","doi":"10.1016/j.aoas.2021.07.001","DOIUrl":"10.1016/j.aoas.2021.07.001","url":null,"abstract":"<div><p>Soil type is a vital determinant of soil fertility because of its characteristic biological, chemical, and physical properties. However, the soil fertility of upland soil is probably changed by different agricultural management practices regardless of soil type. This study investigated the features of soil fertility (bacterial biomass, total carbon (TC), and total nitrogen (TN)) in upland fields in Japan. One thousand soil samples from different soil types were collected from upland fields located on 36 prefectures in Japan. The soil fertility was analyzed using the Soil Fertility Index (SOFIX). There were six soil types included in this study: Organic soils (B), Andosols (D), Lowland soils (F), Red-yellow soils (G), Stagnic soils (H), and Brown Forest soils (I). Of these, the soil types D and F occupied the largest percentage of samples. The values of bacterial biomass, TC, and TN varied greatly, regardless of soil type. The soil fertility does not seem to be characterized by the soil type in upland soils in Japan. The correlations between bacterial biomass and TC (<em>r</em> = 0.23, <em>p</em> < 0.01) and bacterial biomass and TN (<em>r</em> = 0.16, <em>p</em> < 0.01) were relatively weak. Conversely, the correlation between TC and TN (<em>r</em> = 0.68, <em>p</em> < 0.01) was moderate. In soil type D, up to 40% of samples had no bacterial biomass detected and 17% possessed a low bacterial biomass. The effect of the soil types may not be a critical factor in soil fertility in the upland fields.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"66 2","pages":"Pages 101-108"},"PeriodicalIF":5.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.aoas.2021.07.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42426730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sri Suharti , Hafni Oktafiani , Asep Sudarman , Myunggi Baik , Komang Gede Wiryawan
{"title":"Effect of cyanide-degrading bacteria inoculation on performance, rumen fermentation characteristics of sheep fed bitter cassava (Manihot esculenta Crantz) leaf meal","authors":"Sri Suharti , Hafni Oktafiani , Asep Sudarman , Myunggi Baik , Komang Gede Wiryawan","doi":"10.1016/j.aoas.2021.09.001","DOIUrl":"10.1016/j.aoas.2021.09.001","url":null,"abstract":"<div><p>This study aimed to evaluate the performance and fermentation characteristics in the rumen of Garut grade sheep fed bitter cassava (<em>M. esculenta</em> Crantz) leaf meal (BCL) supplemented with cyanide-degrading bacteria (CDB). Fifteen sheep (12 mo with average weight of 18.8 ± 1.02 kg) were placed in individual cages provided experimental diets and free access to water. A completely randomized design applying five treatments and three replicates was used. The treatments were T1 [40% Napier grass:60% concentrate (Con)], T2 [40% Napier grass:45% concentrate: 15% BCL (BCL15)], T3 (BCL15 + CDB), T4 [40% Napier grass:30% concentrate:30% BCL (BCL30)], and T5 (BCL30 + CDB). The data were subjected to analysis of variance and significant differences among treatments were further analyzed using Tukey test. The data of different levels of BCL and CDB inoculation were contrasted using orthogonal contrast test. Feeding BCL 15% and 30% to substitute concentrate ration did not affect feed intake and nutrient digestibility but decreased (<em>P</em> < 0.05) the total volatile fatty acid (VFA), concentration of NH<sub>3,</sub> feed efficiency and body weight gain of sheep. Inoculation of CDB to the sheep fed 15% and 30% BCL did not affect total VFA and NH<sub>3</sub> concentration, but it increased (<em>P</em> < 0.05) body weight and feed efficiency. The methane estimation were similar among treatments. In conclusion, CDB inoculation could detoxify cyanide in BCL and enhanced rumen fermentation profile when BCL 15% and 30%.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"66 2","pages":"Pages 131-136"},"PeriodicalIF":5.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0570178321000361/pdfft?md5=e0cf49fdab1b7860363f978d1749a3ae&pid=1-s2.0-S0570178321000361-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48921137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ahmed Aldughpassi , Sharifa Alkandari , Dina Alkandari , Fatima Al-Hassawi , Jiwan S. Sidhu , Hanan A. Al-Amiri , Ebtihal Al-Salem
{"title":"Effect of psyllium fiber addition on the quality of Arabic flatbread (Pita) produced in a commercial bakery","authors":"Ahmed Aldughpassi , Sharifa Alkandari , Dina Alkandari , Fatima Al-Hassawi , Jiwan S. Sidhu , Hanan A. Al-Amiri , Ebtihal Al-Salem","doi":"10.1016/j.aoas.2021.08.002","DOIUrl":"10.1016/j.aoas.2021.08.002","url":null,"abstract":"<div><p>Psyllium husk (<em>Plantago ovata</em> Forssk.), is an excellent source of natural soluble fiber. Arabic flatbread formulation standardized in a laboratory was scaled up for production in a commercial bakery. The water holding capacity (WHC) of wholegrain wheat flour (WGF), white wheat flour (WWF) and psyllium husk (PS) were found to be 2.9, 2.3 and 59.2 g water/g dry matter, respectively. The total dietary fiber (TDF) for WWF control bread (3.20 ± 0.23%) increased significantly to 5.62 ± 0.16 and 7.56 ± 0.18% with the addition of 3% and 5% psyllium, respectively. The corresponding soluble dietary fiber (SDF) values for these samples were 1.31 ± 0.13, 3.02 ± 0.16 and 4.15 ± 0.14%, respectively. The WWF Arabic flatbread samples also followed a similar trend for TDF and SDF contents with the addition of psyllium husk. At a level of 5% psyllium, the WWF test bread had a puncture force of 2.97 ± 0.16 N (much softer) compared with 3.40 ± 0.35 N for the WWF control bread (0% psyllium), whereas the WGF with 5% psyllium addition had a puncture force of 3.05 ± 0.19 N at the zero day of storage compared to that of 3.94 ± 0.19 N for WGF bread with no psyllium added. The major objective of this study was not only to enhance the soluble dietary fiber content in Arabic flatbread, but also to improve the consumer acceptability of this staple food of this region. Incorporation of psyllium husk did not adversely affect the objective color of crumb or overall consumer acceptability, and produced Arabic flatbread with softer texture, better flexibility (folding) and superior eating quality.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"66 2","pages":"Pages 115-120"},"PeriodicalIF":5.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.aoas.2021.08.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42643254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Asep I.M. Ali , Sofia Sandi , Riswandi , Muhamad N. Rofiq , Suhubdy
{"title":"Effect of feeding Asystasia gangetica weed on intake, nutrient utilization, and gain in Kacang goat","authors":"Asep I.M. Ali , Sofia Sandi , Riswandi , Muhamad N. Rofiq , Suhubdy","doi":"10.1016/j.aoas.2021.10.002","DOIUrl":"10.1016/j.aoas.2021.10.002","url":null,"abstract":"<div><p>A high concentration of nitrate and total nitrogen (N) in Chinese violet (CV) weed (<em>Asystasia gangetica</em>) has a potential benefit as a digestible protein source for ruminant production in humid tropical regions. We conducted a study using twelve Kacang goats in a crossover design to investigate the effect of CV weed on intake, nutrient utilization, and growth. Four dietary treatments were tested: Guinea grass (<em>Panicum maximum</em>) <em>ad libitum</em> (GG), GG <em>ad libitum</em> and CV (1% live weight, LW) (GG + CV), CV <em>ad libitum</em> (CV), and CV <em>ad libitum</em> plus cassava chip (1% LW) (CV + CC) (dry matter, DM basis). No sign of nitrite toxicity was observed when the goat fed CV diet while intake, N retention, and LW gain was higher than goats on the GG and GG + CV diets (<em>P</em> < 0.001). Digestibility of DM was similar to the goats on the GG + CV diet but higher than the goats on the GG diet (<em>P</em> < 0.001). However, the goats on the CV diet had a higher urinary N loss compared to the goats on GG and GG + CV diets (<em>P</em> < 0.001). The cassava supplementation (CV + CC) increased DM digestibility and lowered urinary N loss (<em>P</em> < 0.001). Hence, the CV weed could be fed as a sole diet or as a supplement to the Kacang goat fed a low-quality forage for improvement of nutrients intake, digestibility, and gain, while the urinary N loss could be lowered by cassava supplementation.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"66 2","pages":"Pages 137-141"},"PeriodicalIF":5.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0570178321000385/pdfft?md5=879f64c7b1f61feb5da504b20a4c19f2&pid=1-s2.0-S0570178321000385-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42285611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ayman M. Khalifah , Sara A. Abdalla , Waleed M. Dosoky , Mohamed G. Shehata , Mohamed M. Khalifah
{"title":"Utilization of lemongrass essential oil supplementation on growth performance, meat quality, blood traits and caecum microflora of growing quails","authors":"Ayman M. Khalifah , Sara A. Abdalla , Waleed M. Dosoky , Mohamed G. Shehata , Mohamed M. Khalifah","doi":"10.1016/j.aoas.2021.12.001","DOIUrl":"10.1016/j.aoas.2021.12.001","url":null,"abstract":"<div><p>Lemongrass (<em>Cymbopogon citrates</em>) is grown in tropical and semi-tropical climates. It is an aromatic herb widely used as a flavoring agent in human foods due to its potent flavor. It also includes essential oils, which are important for their antibacterial, antidiarrheal, antifungal, antioxidant, and growth-promoting properties. The experiment was carried out to evaluate lemongrass essential oil (LGEO) supplementation in growing quails. 240 seven days old unsexed Japanese quail (<em>Coturnix japonica</em>) were divided into four groups of 30 quails each. There were ten replicates for each group, with six birds per replicate. The first group received no supplementation, whereas the second, third, and fourth groups received LGEO supplements 0.2, 0.4, and 0.8 g/kg, respectively. Results showed that the growth performance (GP) traits were affected positively by LGEO addition (<em>P</em> ≤ 0.01) at 0.4 g/kg compared to control. All carcass traits were improved significantly (<em>P</em> ≤ 0.01) as affected by LGEO supplementation except carcass percentage. Also, LGEO supplementation enhances meat quality as it improves significantly (<em>P</em> ≤ 0.01) thigh meat water holding capacity (WHC) and pH values. Blood characteristics showed significantly decrease (<em>P</em> ≤ 0.01) on lipid profile, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and malondialdehyde (MDA) as affected by LGEO supplementation, inversely total protein, total antioxidant capacity (TAC), catalase, reduced glutathione (GSH) and superoxide dismutase (SOD) were significantly increased by LGEO supplementation. Likewise, LGEO supplementation improves the concentration of lactobacillus. Results indicated that adding LGEO at 0.4 g/kg diet in quails diets enhance GP, meat quality, blood characteristics, and caecum microorganisms.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"66 2","pages":"Pages 169-175"},"PeriodicalIF":5.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0570178321000403/pdfft?md5=699053ed0b771ea84dc14c7d78528e01&pid=1-s2.0-S0570178321000403-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45541954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Verónica R. Olate-Olave , Luis Guzmán , Xaviera A. López-Cortés , Rafael Cornejo , Fabiane M. Nachtigall , Marnix Doorn , Leonardo Silva Santos , Arturo Bejarano
{"title":"Comparison of Chilean honeys through MALDI-TOF-MS profiling and evaluation of their antioxidant and antibacterial potential","authors":"Verónica R. Olate-Olave , Luis Guzmán , Xaviera A. López-Cortés , Rafael Cornejo , Fabiane M. Nachtigall , Marnix Doorn , Leonardo Silva Santos , Arturo Bejarano","doi":"10.1016/j.aoas.2021.11.001","DOIUrl":"10.1016/j.aoas.2021.11.001","url":null,"abstract":"<div><p>Honey is the most famous natural sweet substance produced by honeybees (<em>Apis mellifera</em>). It contains numerous functional compounds, from which health benefits are obtained. In Chile, the production of honey is associated with its unique biodiversity, but it is exported mostly, as bulk honey. This work aimed to characterize the chemical and biological properties of Chilean honey on a large scale. The use of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) technique, combined with multivariate statistical analysis was introduced to study the chemical profiles of polyfloral honey. The use of a complementary mass spectrometry method allowed the identification of 25 different constituents in the studied honey, including hydrocarbons, acids, esters, glycoside isoprenoids, ketones, and a dihydroxyflavanone. The evaluation of biological properties in Chilean honey was measured in a representative number of polyfloral samples. For this purpose, the total phenolics and flavonoid content were measured in the selected samples. The antioxidant activity of the honey was evaluated through the ferric reducing antioxidant power assay (FRAP) and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. On the other hand, the antibacterial activity was assessed by the dilution technique to determine the minimum inhibitory concentration (MIC) of the honey against seven bacterial strains, including Gram-positive and Gram-negative bacteria. The results demonstrated that the antioxidant and antibacterial activities of Chilean honey are variable and they could have similar properties in comparison with other well-recognized bioactive honey.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"66 2","pages":"Pages 152-161"},"PeriodicalIF":5.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0570178321000397/pdfft?md5=1538c86a93ee2ae5f6ea2bcfb464f1f5&pid=1-s2.0-S0570178321000397-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42852067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}