MCBS Molecular and Cellular Biomedical Sciences最新文献_第2页

Hypoglycaemic, Hypolipidaemic and Antioxidant Properties of Celastrus paniculatus Seed Extract in STZ-induced Diabetic Rats 葡萄籽提取物对stz诱导的糖尿病大鼠的降血糖、降血脂和抗氧化作用

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2023-03-28 DOI: 10.21705/mcbs.v7i1.282

Md. Fahim Ahmad, Md Ali Haidar, Nida Naseem, H. Ahsan, W. Siddiqui

{"title":"Hypoglycaemic, Hypolipidaemic and Antioxidant Properties of Celastrus paniculatus Seed Extract in STZ-induced Diabetic Rats","authors":"Md. Fahim Ahmad, Md Ali Haidar, Nida Naseem, H. Ahsan, W. Siddiqui","doi":"10.21705/mcbs.v7i1.282","DOIUrl":"https://doi.org/10.21705/mcbs.v7i1.282","url":null,"abstract":"Background: Celastrus paniculatus is a herb used in the Ayurvedic system of medicine that has been reported to show multiple pharmacological properties. In this study, we explored the antioxidative, hypolipidaemic and hypoglycaemic potential of C. paniculatus methanolic seed extract (CPMSE) in high-fat diet (HFD)/streptozotocin (STZ)-induced type 2 diabetes mellitus (T2DM) rats.Materials and methods: Seeds of C. paniculatus were extracted in methanol using Soxhlet extraction method. A total of 36 rats were induced with STZ and HFD and treated with glibenclamide or various concentrations of CPMSE. Upon treatment, blood samples were collected and kidney and liver samples were homogenised. Serum biochemical estimation was performed using several diagnostic kits. Protein was estimated by bicinchoninic acid (BCA) method. Oxidative stress was assessed by measuring malondialdehyde level and superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST) activity.Results: CPMSE caused improvements in glucose homeostasis, lipid profile, liver function and oxidative stress parameters in a dose-dependent manner. CPMSE significantly decreased the levels of fasting blood glucose and glycated haemoglobin as well as increased insulin level and total protein content. There was an increase in total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C), triglycerides (TG) levels and reduction in high density lipoprotein-cholesterol (HDL-C) level. There was a decrease in serum levels of serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT) and alkaline phosphatase (ALP). CPMSE decreased LPO and increased CAT, SOD and GST activity.Conclusion: CPMSE has hypoglycaemic, hypolipidaemic and antioxidant properties by reducing the oxidative stress.Keywords: diabetes mellitus, Celastrus paniculatus, antioxidant, phytochemicals, phytonutrients, streptozotocin, high-fat diet","PeriodicalId":53387,"journal":{"name":"MCBS Molecular and Cellular Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87058092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Association of ELMO1 Genetic Polymorphism (rs741301) with the Progression of Diabetic Kidney Disease in Balinese Patients with Type 2 Diabetes Mellitus ELMO1基因多态性(rs741301)与巴厘岛2型糖尿病患者糖尿病肾病进展的关系

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2023-03-28 DOI: 10.21705/mcbs.v7i1.297

Anak Agung Istri Kaila Kirtaniya, A. Lestarini, P. A. N. Permatananda, A. A. S. A. Aryastuti

{"title":"Association of ELMO1 Genetic Polymorphism (rs741301) with the Progression of Diabetic Kidney Disease in Balinese Patients with Type 2 Diabetes Mellitus","authors":"Anak Agung Istri Kaila Kirtaniya, A. Lestarini, P. A. N. Permatananda, A. A. S. A. Aryastuti","doi":"10.21705/mcbs.v7i1.297","DOIUrl":"https://doi.org/10.21705/mcbs.v7i1.297","url":null,"abstract":"Background: Diabetic kidney disease (DKD) is one of diabetes mellitus microvascular complications. Engulfment and cell motility 1 (ELMO1) protein interacts with dedicator of cytokinesis 180 (DOCK180) and cyclooxygenase (COX)-2, which affects gene expression in extracellular matrix (ECM) and causes glomerular damage in several mechanisms, such as ECM accumulation and renal tubules thickening. Single nucleotide polymorphism (SNP) rs741301 is one of the ELMO1 genetic polymorphisms involved in DKD. The aim of this study was to evaluate the association between ELMO1 rs741301 polymorphism and DKD in type 2 diabetes mellitus (T2DM) among Balinese.Materials and methods: This study was an observational analytical study with case-control method. Subjects were divided into control and case groups comprising 40 subjects each. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of DNA from T2DM patients were performed to detect the polymorphism in ELMO1 rs741301. Genotype and allele distribution obtained from this study was analyzed by chi-square (χ2) test and Hardy-Weinberg equilibrium law (p<0.05; CI: 95%).Results: There was no significant difference between genotype or allele distribution of ELMO1 rs741301 with DKD incidence. Genotype AA against GG had odds ratio (OR) of 0.793 (p=0.814), AG against GG had OR of 0.602 (p=0.674), and A allele against G allele had OR of 0.761 (p=0.509).Conclusion: There is no significant relationship between ELMO1 rs741301 polymorphism and DKD in T2DM patients among Balinese.Keywords: ELMO1 gene, diabetic kidney disease, polymorphism","PeriodicalId":53387,"journal":{"name":"MCBS Molecular and Cellular Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79590800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Docking of Citrus amblycarpa Active Compounds against FTO, Leptin, and Resistin Protein 柑橘抗FTO、瘦素和抵抗素活性化合物的分子对接

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2023-03-28 DOI: 10.21705/mcbs.v7i1.295

R. Panghiyangani, J. P. Utami, Muhammad Akbar Baitullah, Novi Dwi Maulida

{"title":"Molecular Docking of Citrus amblycarpa Active Compounds against FTO, Leptin, and Resistin Protein","authors":"R. Panghiyangani, J. P. Utami, Muhammad Akbar Baitullah, Novi Dwi Maulida","doi":"10.21705/mcbs.v7i1.295","DOIUrl":"https://doi.org/10.21705/mcbs.v7i1.295","url":null,"abstract":"Background: Citrus amblycarpa has been known to have various pharmacological activities, such as antioxidants, anticancer, antitumor, hepatoprotective, anti-inflammatory, antidiabetic, antiviral, antibacterial, and antifungal. Hesperidin, naringin, quercetin, rutin, gamma (γ)-aminobutyric acid (GABA), neoeriocitrin, and poncirin from C. amblycarpa were the major constituents that potentially act on some obesity proteins, such as fat mass and obesity-associated (FTO) protein, leptin, and resistin, the emerging targets in the treatment of obesity. This study aimed to investigate the interaction between major active compounds of C. amblycarpa with FTO, leptin and resistin.Materials and methods: The ligands of the docking study were seven major chemical compounds found in peel of C. amblycarpa, i.e., hesperidin, naringin, quercetin, rutin, GABA, neoeriocitrin, and poncirin. FTO, leptin and resistin structure were taken from Protein Data Bank, while the C. amblycarpa compounds were prepared using Open Babel integrated into PyRx 8.0. Molecular docking simulation was performed using Autodock Vina integrated into PyRx 8.0. Virtual prediction and visualization of protein–ligand complexes were analyzed and visualized using Discovery Studio.Results: All major compounds of C. amblycarpa peel used in this study did not have hepatotoxicity and AMES toxicity. Hesperidin had the lowest binding affinity score when interacted with FTO, leptin and resistin compared to other compounds. Moreover, GABA had the highest binding affinity score compared to other compounds.Conclusion: Hesperidin may be a candidate obesity protein antagonist and may have potential as a treatment for obesity.Keywords: Citrus amblycarpa, molecular docking, FTO, leptin, obesity, resistin","PeriodicalId":53387,"journal":{"name":"MCBS Molecular and Cellular Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79684998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between 25(OH)D3 Levels and the Presence of COVID-19 Symptoms 25(OH)D3水平与COVID-19症状之间的关系

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2023-03-28 DOI: 10.21705/mcbs.v7i1.306

Dessy Hermawan, Nurul Aryastuti, Nova Muhani, Syafik Arisandi, Made Prativi

{"title":"Association between 25(OH)D3 Levels and the Presence of COVID-19 Symptoms","authors":"Dessy Hermawan, Nurul Aryastuti, Nova Muhani, Syafik Arisandi, Made Prativi","doi":"10.21705/mcbs.v7i1.306","DOIUrl":"https://doi.org/10.21705/mcbs.v7i1.306","url":null,"abstract":"Background: Recently, there have been studies reporting a relationship between vitamin D levels in the blood and the immune system. This study aimed to analyze the association between vitamin D levels and symptoms of coronavirus disease 2019 (COVID-19).Materials and methods: This study was an analytical survey study with a cross-sectional approach, with lecturers at Universitas Malahayati that have been infected with COVID-19 in 2022 as subjects. Total 47 subjects were included. Subjects were fasted overnight, then blood samples were taken from subjects in the next morning. The blood was centrifuged, then the serum was separated for examination by the direct competitive chemiluminescence immunoassay (CLIA) method using Architect 25-OH Vitamin D Reagent. Data was analyzed using logistic regression.Results: None of the subjects had normal blood levels of 25(OH)D3 and almost half (48.9%) of the subjects had symptoms when infected with COVID-19. There was a significant relationship between the level of 25(OH)D3 (p=0.001) and the status of the COVID-19 vaccine (p=0.013) with the presence of symptoms in COVID-19 patients.Conclusion: The lower the level of 25(OH)D3 in the blood and the more incomplete the COVID-19 vaccine, the greater the onset potential of COVID-19 symptoms. It is necessary to maintain vitamin D intake and increase the coverage of the COVID-19 vaccine, especially at booster doses.Keywords: vitamin D, COVID-19, vaccination, health protocol","PeriodicalId":53387,"journal":{"name":"MCBS Molecular and Cellular Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85779245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

D-dimer as a Potential Biomarker of Severity in Children Confirmed with COVID-19 d -二聚体作为COVID-19确诊儿童严重程度的潜在生物标志物

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2022-11-02 DOI: 10.21705/mcbs.v6i3.276

Hanum Ferdian, Rustam Siregar, A. Moelyo

{"title":"D-dimer as a Potential Biomarker of Severity in Children Confirmed with COVID-19","authors":"Hanum Ferdian, Rustam Siregar, A. Moelyo","doi":"10.21705/mcbs.v6i3.276","DOIUrl":"https://doi.org/10.21705/mcbs.v6i3.276","url":null,"abstract":"Background: Coronavirus disease 2019 (COVID-19) in children spreads easily and has a relatively high incidence. Severe complications in children confirmed with COVID-19 are thought to be related to the multisystem inflammatory syndrome, which is associated with coagulation disorders. D-dimer is a fibrin degradation end product which is easy to examine, affordable, fast and reliable. This study investigated the potency of D-dimer levels as a biomarker and assessed optimal cut-off value of D-dimer on severity of COVID-19 in children. Materials and methods: An analytical observational study with a cross-sectional design was conducted in children aged 1-18 years confirmed to have mild, moderate or severe COVID-19 who were treated in the isolation room of Dr. Moewardi Hospital, Surakarta, Indonesia from September 2021 to February 2022. Statistical analysis was conducted using Mann- Whitney test and p<0.05 was considered as statistically significant. The cut-off value of D-dimer was determined with the receiver operating characteristic (ROC) curve. Results: There were 39 children with COVID-19. They were in mild (n=14; 35.9%), moderate (n=19; 48.7%) and severe (n= 6; 15.4%) stages. There were significant differences in D-dimer levels between mild and moderate stages (p=0.001), and mild and severe stages (p=0.001). No significant difference in D-dimer levels between moderate and severe stages (p=0.162). The cut-off value of D-dimer was 485 μg/mL with 92% sensitivity and 71.4% specificity. Conclusion: D-dimer can be used as a potential biomarker of severity in children with COVID-19.Keywords: D-dimer, COVID-19, severity, children","PeriodicalId":53387,"journal":{"name":"MCBS Molecular and Cellular Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89574827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mucin Level as a Potential Biomarker for Breast Cancer Diagnosis 黏液蛋白水平作为乳腺癌诊断的潜在生物标志物

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2022-11-02 DOI: 10.21705/mcbs.v6i3.238

Tagwa Sayed Abdihalim, A. Idris

{"title":"Mucin Level as a Potential Biomarker for Breast Cancer Diagnosis","authors":"Tagwa Sayed Abdihalim, A. Idris","doi":"10.21705/mcbs.v6i3.238","DOIUrl":"https://doi.org/10.21705/mcbs.v6i3.238","url":null,"abstract":"Background: Breast cancer is the second leading cause of cancer death and a health problem worldwide. Secreted mucins are upregulated in ductal adenocarcinoma of the breast, however, the use of mucin as breast cancer biomarker has not been established before. This study aimed to determine the use of mucin level as a potential biomarker for breast cancer diagnosis. Materials and methods: This was a retrospective, cross-section study involving 40 women subjects with breast cancer. Mucin level was examined with a combination of Alcian blue/periodic acid Schiff (AB/PAS) technique applied to each specimen. The results obtained were statistically analyzed using SPSS.Results: Results of neutral mucin detection showed that among breast cancer subjects, 16 cases (40%) were neutral mucin score (+), 23 cases (57.5%) were neutral mucin score (++), and 1 case (2.5%) was neutral mucin score (+++). Meanwhile, 10 cases (25%) were acid mucin negative, 17 cases (42.5%) were acid mucin score (+), 11 cases (27.5%) were acid mucin score (++), and 2 cases (5%) were acid mucin score (+++). The most frequent type of mucin was the combination of acid and neutral mucin (30 cases; 75%) and neutral mucin were 10 cases (25%). Conclusion: Detection of mucin level can be used as an alternative technique for the diagnosis of breast cancer complementary to other types of special stains. Keywords: AB, PAS, breast cancer, histological grade, mucin level","PeriodicalId":53387,"journal":{"name":"MCBS Molecular and Cellular Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84003784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Safety and Efficacy of Mesenchymal Stem Cells in Burn Therapy: Systematic Review 间充质干细胞在烧伤治疗中的安全性和有效性:系统综述

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2022-11-02 DOI: 10.21705/mcbs.v6i3.252

Theresia Dini, Yudhi Nugraha, R. Revina, K. Karina

{"title":"Safety and Efficacy of Mesenchymal Stem Cells in Burn Therapy: Systematic Review","authors":"Theresia Dini, Yudhi Nugraha, R. Revina, K. Karina","doi":"10.21705/mcbs.v6i3.252","DOIUrl":"https://doi.org/10.21705/mcbs.v6i3.252","url":null,"abstract":"The experimental research on the use of mesenchymal stem cells (MSCs) for burn therapy has been published several times. However, current clinical procedure remains a challenging discussion. This systematic review assesses the safety and efficacy of administering mesenchymal stem cells (MSCs) to burns and determines the most effective source of MSCs for burn therapy. We reviewed several studies through PubMed, Google Scholar, Science Direct, and DOAJ online databases. PRISMA-P 2020 method was used based on inclusion and exclusion criteria that were re-selected through Joanna Briggs Institute (JBI) Critical Appraisal Tools. Results from 13 articles showed that MSCs are safe for burn therapy with minimal side effects/complications and have the potential to repair tissue and accelerate burn healing through several mechanisms. The treatment of MSCs in burns is influenced by donor characteristics and related to the severity and area of the burn. It can be concluded that the administration of MSCs is safe and effective in burn therapy. Keywords: burns, mesenchymal stem cells, therapeutic safety, therapeutic efficacy, wound healing","PeriodicalId":53387,"journal":{"name":"MCBS Molecular and Cellular Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91196204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

In silico Study of Essential Oil of Bambusa vulgaris Leaves as an Anti Beta-lactamase Compound 竹叶挥发油抗内酰胺酶化合物的硅片研究

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2022-11-02 DOI: 10.21705/mcbs.v6i3.278

Pragati Wira Anggini, S. Amanina, Salwa Rainha Asyura, Romario Dion

{"title":"In silico Study of Essential Oil of Bambusa vulgaris Leaves as an Anti Beta-lactamase Compound","authors":"Pragati Wira Anggini, S. Amanina, Salwa Rainha Asyura, Romario Dion","doi":"10.21705/mcbs.v6i3.278","DOIUrl":"https://doi.org/10.21705/mcbs.v6i3.278","url":null,"abstract":"Background: Klebsiella pneumoniae is known as an extended spectrum beta (β)-lactamases (ESBLs)-producing bacteria, which produces enzymes that cause resistance to β-lactam antibiotics by degrading β-lactam ring. A solution is needed to prevent the degradation of the β-lactam ring. In this in silico study, combining β-lactam antibiotics with secondary metabolites has the possibility to inhibit the active site of the β-lactamase enzyme. This study aimed to explore the potential of the essential oil of yellow bamboo (Bambusa vulgaris) leaves as inhibitors of β-lactamase. Materials and methods: This research was conducted by simulating molecular docking to determine the interaction of ligands with proteins, pharmacological tests of compounds based on the Lipinski’s rule of five, and ligand toxicity tests with pkCSM. Results: The free bond energy values (∆G) were in the range of -4.3 to -8.0 kcal/mol. The ligands with the best ∆G value were sulfur pentafluoride (-8.0 kcal/mol), squalene (-7.3 kcal/mol), 3-aminodibenzofuran (-7.1 kcal/mol), and 2- monolaurin (-5.5 kcal/mol). Secondary metabolites from the essential oil of B. vulgaris leaves fulfilled Lipinski’s rule of five, so that oral use can be carried out except for squalene and tridecane. Conclusion: Secondary metabolite compounds in the essential oil that have potential as oral drugs based on the Lipinski pharmacological test and the pkCSM toxicity test are dipivaloylmethane, β-ocimene, 2-monolaurin, and undecane. Keywords: β-lactamase, Bambusa vulgaris, essential oil, Klebsiella pneumoniae","PeriodicalId":53387,"journal":{"name":"MCBS Molecular and Cellular Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91060597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Antimicrobial Activity of Ethanol Extract of Centella asiatica Leaves on Proteus mirabilis, Proteus vulgaris, and Yersinia enterocolitica in vitro 积雪草叶乙醇提取物对奇异变形杆菌、寻常变形杆菌和小肠结肠炎耶尔森菌的体外抑菌活性研究

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2022-11-02 DOI: 10.21705/mcbs.v6i3.266

Salwa Putri Qurrotuaini, Nurul Wiqoyah, Arifa Mustika

{"title":"Antimicrobial Activity of Ethanol Extract of Centella asiatica Leaves on Proteus mirabilis, Proteus vulgaris, and Yersinia enterocolitica in vitro","authors":"Salwa Putri Qurrotuaini, Nurul Wiqoyah, Arifa Mustika","doi":"10.21705/mcbs.v6i3.266","DOIUrl":"https://doi.org/10.21705/mcbs.v6i3.266","url":null,"abstract":"Background: Centella asiatica leaves ethanol extract (CALEE) has higher concentration compared to other structures within the plant. The extract contains alkaloids, saponins and flavonoids, which play an active role as antioxidant and antibacterial. Current study aimed to determine the effect of CALEE on Proteus mirabilis, Proteus vulgaris, and Yersinia enterocolitica, Gram-negative bacteria that cause diarrhea. Materials and methods: Simplicia of C. asiatica leaves was dissolved in 96% ethanol and macerated to get condensed extract, which then produced a concentration of 1 g/mL. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined to assess the effectiveness of CALEE on P. mirabilis, P. vulgaris, and Y. enterocolitica. MIC was determined through serial dilution test with Mueller Hinton broth media. After incubation, the bacteria were streaked on nutrient agar or McConkey agar to determine the MBC. Results: The MIC value of CALEE could not be determined since the color of CALEE was dark, hence the turbidity could not be compared. CALEE had the same MBC value (0.25 g/mL) in all bacteria species used in this study. Conclusion: CALEE is effective against Gram-negative bacteria, such as P. mirabilis, P. vulgaris, and Y. enterocolitica. Further research is needed, especially in vivo experiments and evaluation of the cytotoxicity effect of CALEE. Keywords: Centella asiatica, Proteus mirabilis, Proteus vulgaris, Yersinia enterocolitica, antibacterial","PeriodicalId":53387,"journal":{"name":"MCBS Molecular and Cellular Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87627362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methanol Extract of Katuk (Sauropus androgynus) Leaves as an Anti-inflammatory Agent: Animal Study in Carrageenan-induced Rat Models of Inflammation 雌雄同体龙(Sauropus androgynus)叶甲醇提取物抗炎作用:卡拉胶诱导大鼠炎症模型的动物研究

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2022-11-02 DOI: 10.21705/mcbs.v6i3.263

Desy Andari, Farah Ibnu Khan, Safira Idofia Jakfar

{"title":"Methanol Extract of Katuk (Sauropus androgynus) Leaves as an Anti-inflammatory Agent: Animal Study in Carrageenan-induced Rat Models of Inflammation","authors":"Desy Andari, Farah Ibnu Khan, Safira Idofia Jakfar","doi":"10.21705/mcbs.v6i3.263","DOIUrl":"https://doi.org/10.21705/mcbs.v6i3.263","url":null,"abstract":"Background: Inflammation is a response in the human body to survive during infection, injury and tissue damage. Acute inflammation causes edema and polymorphonuclear neutrophils (PMNs) release. Sauropus androgynus leaves which contain flavonoids, tannins, saponins, steroids and triterpenoids may have anti-inflammatory properties. These compounds can be extracted with methanol. This research aimed to determine the anti-inflammatory effect of S. androgynus leaves methanol extract (SALME) on carrageenan induced-rats. Materials and methods: True experimental study was conducted using 12 Wistar rats. Rats were induced with carrageenan subcutaneously on the plantar pedis. Carrageenan-induced rats were treated with/without various doses of SALME. Edema volume was measured with a plethysmometer. The plantar pedis tissues were collected and stained with haematoxylin- eosin (HE) staining, then PMNs were observed and counted under a light microscope. All data were analyzed by one-way ANOVA, Bonferroni post hoc test, Pearson correlation and linear regression. Results: SALME had significant effects on the volume of edema (p=0.000) and the number of PMNs (p=0.000). The most effective dose to reduce the edema volume and decrease the PMNs cell number was 37.80 mg/200 g body weight (BW). SALME doses may affect 91.0% of edema volume and 89.2% of PMNs cell number. Edema volume had a significant, robust correlation (92.3%) with PMNs cell number. Conclusion: SALME is confirmed to have an anti-inflammatory activity by reducing the edema volume and decreasing the PMNs cell number. Keywords: Sauropus androgynus, methanol extract, anti-inflamamatory, carrageenan, plantar pedis, rats","PeriodicalId":53387,"journal":{"name":"MCBS Molecular and Cellular Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74851566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0