Md. Fahim Ahmad, Md Ali Haidar, Nida Naseem, H. Ahsan, W. Siddiqui
{"title":"葡萄籽提取物对stz诱导的糖尿病大鼠的降血糖、降血脂和抗氧化作用","authors":"Md. Fahim Ahmad, Md Ali Haidar, Nida Naseem, H. Ahsan, W. Siddiqui","doi":"10.21705/mcbs.v7i1.282","DOIUrl":null,"url":null,"abstract":"Background: Celastrus paniculatus is a herb used in the Ayurvedic system of medicine that has been reported to show multiple pharmacological properties. In this study, we explored the antioxidative, hypolipidaemic and hypoglycaemic potential of C. paniculatus methanolic seed extract (CPMSE) in high-fat diet (HFD)/streptozotocin (STZ)-induced type 2 diabetes mellitus (T2DM) rats.Materials and methods: Seeds of C. paniculatus were extracted in methanol using Soxhlet extraction method. A total of 36 rats were induced with STZ and HFD and treated with glibenclamide or various concentrations of CPMSE. Upon treatment, blood samples were collected and kidney and liver samples were homogenised. Serum biochemical estimation was performed using several diagnostic kits. Protein was estimated by bicinchoninic acid (BCA) method. Oxidative stress was assessed by measuring malondialdehyde level and superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST) activity.Results: CPMSE caused improvements in glucose homeostasis, lipid profile, liver function and oxidative stress parameters in a dose-dependent manner. CPMSE significantly decreased the levels of fasting blood glucose and glycated haemoglobin as well as increased insulin level and total protein content. There was an increase in total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C), triglycerides (TG) levels and reduction in high density lipoprotein-cholesterol (HDL-C) level. There was a decrease in serum levels of serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT) and alkaline phosphatase (ALP). CPMSE decreased LPO and increased CAT, SOD and GST activity.Conclusion: CPMSE has hypoglycaemic, hypolipidaemic and antioxidant properties by reducing the oxidative stress.Keywords: diabetes mellitus, Celastrus paniculatus, antioxidant, phytochemicals, phytonutrients, streptozotocin, high-fat diet","PeriodicalId":53387,"journal":{"name":"MCBS Molecular and Cellular Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Hypoglycaemic, Hypolipidaemic and Antioxidant Properties of Celastrus paniculatus Seed Extract in STZ-induced Diabetic Rats\",\"authors\":\"Md. Fahim Ahmad, Md Ali Haidar, Nida Naseem, H. Ahsan, W. Siddiqui\",\"doi\":\"10.21705/mcbs.v7i1.282\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: Celastrus paniculatus is a herb used in the Ayurvedic system of medicine that has been reported to show multiple pharmacological properties. In this study, we explored the antioxidative, hypolipidaemic and hypoglycaemic potential of C. paniculatus methanolic seed extract (CPMSE) in high-fat diet (HFD)/streptozotocin (STZ)-induced type 2 diabetes mellitus (T2DM) rats.Materials and methods: Seeds of C. paniculatus were extracted in methanol using Soxhlet extraction method. A total of 36 rats were induced with STZ and HFD and treated with glibenclamide or various concentrations of CPMSE. Upon treatment, blood samples were collected and kidney and liver samples were homogenised. Serum biochemical estimation was performed using several diagnostic kits. Protein was estimated by bicinchoninic acid (BCA) method. Oxidative stress was assessed by measuring malondialdehyde level and superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST) activity.Results: CPMSE caused improvements in glucose homeostasis, lipid profile, liver function and oxidative stress parameters in a dose-dependent manner. CPMSE significantly decreased the levels of fasting blood glucose and glycated haemoglobin as well as increased insulin level and total protein content. There was an increase in total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C), triglycerides (TG) levels and reduction in high density lipoprotein-cholesterol (HDL-C) level. There was a decrease in serum levels of serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT) and alkaline phosphatase (ALP). CPMSE decreased LPO and increased CAT, SOD and GST activity.Conclusion: CPMSE has hypoglycaemic, hypolipidaemic and antioxidant properties by reducing the oxidative stress.Keywords: diabetes mellitus, Celastrus paniculatus, antioxidant, phytochemicals, phytonutrients, streptozotocin, high-fat diet\",\"PeriodicalId\":53387,\"journal\":{\"name\":\"MCBS Molecular and Cellular Biomedical Sciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-03-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"MCBS Molecular and Cellular Biomedical Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21705/mcbs.v7i1.282\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"MCBS Molecular and Cellular Biomedical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21705/mcbs.v7i1.282","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
摘要
背景:蛇芹是一种在阿育吠陀医学系统中使用的草药，据报道显示出多种药理特性。在本研究中，我们探讨了高脂肪饮食(HFD)/链脲佐菌素(STZ)诱导的2型糖尿病(T2DM)大鼠的抗氧化、降血脂和降糖潜能。材料与方法:采用索氏提取法，在甲醇溶剂中提取金银花种子。36只大鼠分别用STZ和HFD诱导，并用格列本脲或不同浓度的CPMSE治疗。治疗后，采集血液样本，肾脏和肝脏样本均质化。使用几种诊断试剂盒进行血清生化评估。蛋白含量采用比辛胆酸(BCA)法测定。通过测定丙二醛水平、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽- s -转移酶(GST)活性来评估氧化应激。结果:CPMSE以剂量依赖的方式改善葡萄糖稳态、脂质谱、肝功能和氧化应激参数。CPMSE显著降低空腹血糖和糖化血红蛋白水平，增加胰岛素水平和总蛋白含量。总胆固醇(TC)、低密度脂蛋白-胆固醇(LDL-C)、甘油三酯(TG)水平升高，高密度脂蛋白-胆固醇(HDL-C)水平降低。血清谷氨酸丙酮转氨酶(SGPT)、谷氨酸草酰乙酸转氨酶(SGOT)和碱性磷酸酶(ALP)水平降低。CPMSE降低LPO，提高CAT、SOD和GST活性。结论:CPMSE具有降低氧化应激的降血糖、降血脂和抗氧化作用。关键词:糖尿病，芹菜，抗氧化剂，植物化学物质，植物营养素，链脲佐菌素，高脂饮食
Hypoglycaemic, Hypolipidaemic and Antioxidant Properties of Celastrus paniculatus Seed Extract in STZ-induced Diabetic Rats
Background: Celastrus paniculatus is a herb used in the Ayurvedic system of medicine that has been reported to show multiple pharmacological properties. In this study, we explored the antioxidative, hypolipidaemic and hypoglycaemic potential of C. paniculatus methanolic seed extract (CPMSE) in high-fat diet (HFD)/streptozotocin (STZ)-induced type 2 diabetes mellitus (T2DM) rats.Materials and methods: Seeds of C. paniculatus were extracted in methanol using Soxhlet extraction method. A total of 36 rats were induced with STZ and HFD and treated with glibenclamide or various concentrations of CPMSE. Upon treatment, blood samples were collected and kidney and liver samples were homogenised. Serum biochemical estimation was performed using several diagnostic kits. Protein was estimated by bicinchoninic acid (BCA) method. Oxidative stress was assessed by measuring malondialdehyde level and superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST) activity.Results: CPMSE caused improvements in glucose homeostasis, lipid profile, liver function and oxidative stress parameters in a dose-dependent manner. CPMSE significantly decreased the levels of fasting blood glucose and glycated haemoglobin as well as increased insulin level and total protein content. There was an increase in total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C), triglycerides (TG) levels and reduction in high density lipoprotein-cholesterol (HDL-C) level. There was a decrease in serum levels of serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT) and alkaline phosphatase (ALP). CPMSE decreased LPO and increased CAT, SOD and GST activity.Conclusion: CPMSE has hypoglycaemic, hypolipidaemic and antioxidant properties by reducing the oxidative stress.Keywords: diabetes mellitus, Celastrus paniculatus, antioxidant, phytochemicals, phytonutrients, streptozotocin, high-fat diet
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
