Vaccine Research最新文献_第2页

The predominance of Newcastle disease virus genotype VII: genome diversity or poor cross-immunity of non-matched vaccines 新城疫病毒基因型VII的优势：基因组多样性或不匹配疫苗的交叉免疫差

Vaccine Research Pub Date : 2021-12-01 DOI: 10.52547/vacres.8.2.4

S. Shahsavandi, M. Ebrahimi, Majid Tebianain

引用次数: 0

Lessons Learned from Global Administration of Sinopharm (BBIBP-CorV) Vaccine and Its Efficacy against COVID-19 in Older People 中国医药集团全球管理(BBIBP-CorV)疫苗的经验教训及其对老年人的抗COVID-19效果

Vaccine Research Pub Date : 2021-12-01 DOI: 10.52547/vacres.8.2.1

S. Teo

引用次数: 0

Immunoinformatics Approach for Designing an HPV Epitope-Based Vaccine Candidate Harboring Built-in Adjuvants 利用免疫信息学方法设计含有内建佐剂的HPV表位候选疫苗

Vaccine Research Pub Date : 2021-12-01 DOI: 10.52547/vacres.8.2.28

Maryam Mashhadi Abolghasem Shirazi, A. Arashkia, S. Haghighat, F. Roohvand, S. M. Sadat

{"title":"Immunoinformatics Approach for Designing an HPV Epitope-Based Vaccine Candidate Harboring Built-in Adjuvants","authors":"Maryam Mashhadi Abolghasem Shirazi, A. Arashkia, S. Haghighat, F. Roohvand, S. M. Sadat","doi":"10.52547/vacres.8.2.28","DOIUrl":"https://doi.org/10.52547/vacres.8.2.28","url":null,"abstract":"against using tools. Methods: The HPV16 RG-1 epitope linked to built-in adjuvants including the D1 domain of flagellin as agonists, and a tetanus toxoid epitope for induction of immune responses. Using immunoinformatic tools, the immunological characteristics of the construct were evaluated. In the first step, MHC-I and II binding, CD4 + T cell immunogenicity prediction, and in the second step, immunogenicity simulation of the construct were investigated. Results: MHC-I and II predicted epitopes showed a high potentiality to bind to mice and human MHC alleles. The results of the binding of the RG-1 epitope to MHC-I and MHC-II showed that RG1 could induce humoral and cellular immune response while fused to three built-in adjuvants. Also, the CD4 + immunogenicity assessment results predicted that several epitopes in the designed construct, including epitopes of D1 domain and tetanus toxoid P2 epitope, behaved as potentially strong Th inducers. The immunogenicity simulation results showed that the construct could potentially provide sufficient antigen, and induce suitable humoral and cellular immune responses. Conclusion: The development of new vaccine strategies has been the focus of several studies. The results showed that the designed construct can potentially provide an effective model for developing a preventive vaccine candidate against a variety of HPV genotypes.","PeriodicalId":52727,"journal":{"name":"Vaccine Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41678483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of Polyomavirus and Herpesvirus Recombinant Glycoproteins with Immunoreactivity Using a Rapid and Novel Expression System in Insect Cells for Applications in Vaccines and Serological Assays 在昆虫细胞中使用快速新的表达系统生产具有免疫反应性的多瘤病毒和疱疹病毒重组糖蛋白，用于疫苗和血清学检测

Vaccine Research Pub Date : 2021-06-01 DOI: 10.52547/vacres.8.1.67

B. Abedi Kiasari, H. Najafi

{"title":"Production of Polyomavirus and Herpesvirus Recombinant Glycoproteins with Immunoreactivity Using a Rapid and Novel Expression System in Insect Cells for Applications in Vaccines and Serological Assays","authors":"B. Abedi Kiasari, H. Najafi","doi":"10.52547/vacres.8.1.67","DOIUrl":"https://doi.org/10.52547/vacres.8.1.67","url":null,"abstract":"Introduction: Although, conventional methods for the expression of polyomaviruses and herpesviruses recombinant proteins for serological assays and vaccine developments in baculoviruses are well established, the manipulations are laborious and time consuming. Methods: A new expression system based on plasmid was used to express two polyomaviruses major capsid protein VP1 (JCV VP1 and BKV VP1), and two herpesviruses glycoproteins (HSV-1 gD and VZV gE) in insect cells. A ligation independent cloning (LIC) was applied to generate the recombinant plasmids. Transfection of Sf9 insect cells were performed using the recombinants. The produced proteins were analysed using SDS-PAGE, immunofluorescence, and immunoblotting. Results: JCV-VP1, BKV-VP1, VZV-gE and HSV-1gD were successfully expressed in the insect cells, 48 h post-infection and detected in cytoplasm and cell membranes with immunoreactivity. This plasmid based expression system took 5 days to express the protein. Conclusion: The plasmid based expression system in insect cells was highly efficient and would be ideal for rapid expression of polyomaviruses and herpesviruses proteins in insect cells to be potentially used in applications such as vaccine components and serological assays.","PeriodicalId":52727,"journal":{"name":"Vaccine Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42987484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction and Rescue of a Rabies Virus with Duplicated Glycoprotein Gene 带重复糖蛋白基因的狂犬病毒的构建与抢救

Vaccine Research Pub Date : 2021-06-01 DOI: 10.52547/vacres.8.1.104

A. Alamdary, A. Gholami, M. Azizi, Z. Noormohammadi

引用次数: 0

Cutaneous Reactions after COVID-19 vaccines COVID-19疫苗后的皮肤反应

Vaccine Research Pub Date : 2021-06-01 DOI: 10.52547/vacres.8.1.98

Alicia Wan Yan Poh, S. Teo

{"title":"Cutaneous Reactions after COVID-19 vaccines","authors":"Alicia Wan Yan Poh, S. Teo","doi":"10.52547/vacres.8.1.98","DOIUrl":"https://doi.org/10.52547/vacres.8.1.98","url":null,"abstract":"This article provides an overview of cutaneous reactions after administration of COVID19 vaccines. Cutaneous reactions post COVID-19 trials range from acute and immediate reactions to delayed reactions. The suspected triggers for the hypersensitivity reactions are the inactive ingredients, such as polyethylene glycol in mRNA vaccines and polysorbate 80 in AstraZeneca. Localized or injection-site reactions are generally self-limiting and occur within seven days. Younger, female patients were more likely to report injection-site reactions. Cutaneous reactions after the second dose occurred earlier than after the first dose. Delayed large local reactions or „COVID arms‟ have been reported at least seven days post-vaccination and generally resolve within two weeks. However, this was reported as early as four days post-AstraZeneca vaccination. Other dermatological reactions, such as pityriasis rosea-like eruptions and flares of existing cutaneous conditions occurred in mRNA and AstraZeneca recipients but not with Sinopharm. Screening questions may be used to risk stratifying vaccine recipients into low, medium or high risk of developing severe allergic reactions. Skin testing may be considered for high-risk category patients. However, negative skin testing does not rule out a subsequent allergic response. Delayed cutaneous reactions may be misdiagnosed as cellulitis, resulting in unnecessary treatment with antibiotics. such as vitamins, other vaccines, anti-arrhythmics, anti-diabetics, thrombolytics, anti-cancer agents, contraceptives, creams, ointments","PeriodicalId":52727,"journal":{"name":"Vaccine Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46209388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pulmonary Thromboembolism Followed by Deep Vein Thrombosis In A Young Man With G6PD Deficiency After ChAd0x1 nCoV-19 Vaccine Administration 注射ChAd0x1 nCoV-19疫苗后G6PD缺乏症青年患者肺血栓栓塞并发深静脉血栓形成

Vaccine Research Pub Date : 2021-06-01 DOI: 10.52547/vacres.8.1.88

A. Kiani, A. Abedini, Z. Rouhani, Mohammadhossein Banitorfi, A. Saeidi, H. Zaheri

引用次数: 0

Design and Recombinant Expression of a multiepitope Vaccine Candidate Against Pathogenic Species of Shigella 志贺氏菌致病性多表位候选疫苗的设计与重组表达

Vaccine Research Pub Date : 2021-06-01 DOI: 10.52547/vacres.8.1.18

A. Namvar, A. Hajizade, S. Nazarian, Davoud Sadeghi, M. R. Akbari, Yousof Tarverdizade

引用次数: 0

Comparative Sequence Analysis, Pathogenicity and Immunogenicity of Attenuated Fowl Adenovirus Isolates as Experimental Vaccines against Inclusion Body Hepatitis of Commercial Broiler Chickens 鸡腺病毒减毒分离物作为商品肉鸡包涵体肝炎实验疫苗的比较序列分析、致病性和免疫原性

Vaccine Research Pub Date : 2021-06-01 DOI: 10.52547/vacres.8.1.81

Norfitriah Mohamed Sohaimi, M. Hair-Bejo

{"title":"Comparative Sequence Analysis, Pathogenicity and Immunogenicity of Attenuated Fowl Adenovirus Isolates as Experimental Vaccines against Inclusion Body Hepatitis of Commercial Broiler Chickens","authors":"Norfitriah Mohamed Sohaimi, M. Hair-Bejo","doi":"10.52547/vacres.8.1.81","DOIUrl":"https://doi.org/10.52547/vacres.8.1.81","url":null,"abstract":"and compared based on sequence analysis of and genes. Their pathogenicities and determined in the chickens. Groups of were inoculated with 0.5ml chicken-embryonated-derived attenuated FAdV isolates via oral and IP routes and trachea, and gizzard collected at days 3, 7, 14 and 21post-inoculation. Results: Molecular analysis revealed both isolates had 99.1% and 97.3% homologies in the L1 loop region of hexon gene and knob region of fiber gene, respectively. Molecular changes in UPM1137E20 were prominent in the knob of fiber gens with 3 amino acid changes, while for UPM1137CEL35, notable in the L1 loop region with 3 amino acid changes. It was demonstrated that both attenuated isolates are non-pathogenic and safe in commercial broiler chickens. Neither gross nor histopathological lesions were recorded in all tested groups. Both isolates induced high antibody response significantly via intraperitoneal route when compared to the control. Conclusion: UPM1137E20 isolate had a high potential to be further evaluated as a live attenuated vaccine against viral poultry diseases such as IBH.","PeriodicalId":52727,"journal":{"name":"Vaccine Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46246150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Assessment of Peste Des Petits Ruminants Antibodies in Vaccinated Yankasa Pregnant Ewes from Nigeria and the Duration of Maternal Immunity in Their Lambs 尼日利亚Yankasa妊娠母羊接种小反刍兽疫抗体及母羊羔羊免疫持续时间的评估

Vaccine Research Pub Date : 2021-06-01 DOI: 10.52547/vacres.8.1.47

O. Olaolu, H. Kazeem, J. Adamu, T. Markus, T. Woma

{"title":"Assessment of Peste Des Petits Ruminants Antibodies in Vaccinated Yankasa Pregnant Ewes from Nigeria and the Duration of Maternal Immunity in Their Lambs","authors":"O. Olaolu, H. Kazeem, J. Adamu, T. Markus, T. Woma","doi":"10.52547/vacres.8.1.47","DOIUrl":"https://doi.org/10.52547/vacres.8.1.47","url":null,"abstract":"examined using Competitive ELISA (c-ELISA) for the presence of specific PPR-N antibodies. Results: The analysed result showed that there was significant difference ( P < 0.05) in the mean PPRV-N specific antibody c-ELISA values (0-13) before vaccination and the percentage competition protective values (> 50%). However, no significant difference ( p > 0.05) post-vaccination in both pregnant and non-pregnant ewes was observed throughout the period of the study with mean PPRV-N specific c-ELISA antibodies of 72-86 and 52-86, respectively. The mean PPRV-N specific antibodies values were maintained within the protective value (> 50 %). The result of this study also showed that there was significant difference ( P < 0.05) with mean PPRV-N specific c-ELISA antibodies (17.3-29.4; 87.5%) of lambs born to vaccinated pregnant Yankassa ewes from 8 weeks. Conclusion: This study showed that vaccination does not affect pregnancy with Nigeria 75/1 strain of PPR vaccine in ewes as there was no record of abortion. There was a rapid PPR maternal antibody decay in lambs from the 8th week of age as it was observed that at age 10 weeks, only 37.5 % of the lambs had protective titre. It is therefore recommended that lambs can be vaccinated at 9 th week to avoid the window of susceptibility to PPR virus infection.","PeriodicalId":52727,"journal":{"name":"Vaccine Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44833913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2