志贺氏菌致病性多表位候选疫苗的设计与重组表达

Vaccine Research Pub Date : 2021-06-01 DOI:10.52547/vacres.8.1.18

A. Namvar, A. Hajizade, S. Nazarian, Davoud Sadeghi, M. R. Akbari, Yousof Tarverdizade

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引用次数: 0

摘要

结果：利用聚合酶链式反应证实了该基因的亚克隆。基因表达分析表明，所需蛋白质具有合适的表达。蛋白质印迹分析证实了重组蛋白的表达。结论：表达并纯化的含有致病性志贺菌常见抗原主要表位的多表位重组蛋白，可作为设计志贺菌病多表位候选疫苗的第一步。

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Design and Recombinant Expression of a multiepitope Vaccine Candidate Against Pathogenic Species of Shigella

Results: The sub-cloning of the gene was confirmed using PCR reaction. Gene expression analysis showed that the desired protein had a suitable expression. Western blotting analysis confirmed the expression of the recombinant protein. Conclusion: The expressed and purified multi-epitope recombinant protein, containing the main epitopes of the common antigens of pathogenic Shigella species could be achieved as the first step to design a multiepitope vaccine candidate against shigellosis.

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来源期刊

Vaccine Research

自引率

0.00%

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审稿时长

15 weeks