Journal of Tropical Biodiversity and Biotechnology最新文献

{"title":"Stomata characters of sugarcane (Saccharum officinarum L.) mutants of GMP3 variety at PT Gunung Madu Plantations, Lampung, Indonesia","authors":"Mahfut Mahfut, Putri Kendari, Admi Syarif, Sri Wahyuningsih, Endah Susiyanti","doi":"10.22146/jtbb.79860","DOIUrl":"https://doi.org/10.22146/jtbb.79860","url":null,"abstract":"The induction of colchicine mutations is one method of breeding. PT Gunung Madu Plantations, for example, has induced mutations of commercial sugarcane (Saccharum officinarum L.) varieties, however, investigations on the impact of colchicine on stomatal characters have received less attention. Therefore, this study aimed to analyse the stomata character of 21 sugarcane mutants of the GMP3 variety at PT Gunung Madu Plantations, Lampung, Indonesia with a focused look at stomata aperture width, stomata length and width, number of stomata, stomatal density, and stomata index. The collected data were analysed using cluster and Principal Component Analysis (PCA) through MVSP software. This study showed that all GMP3 mutants had Graminae-type stomata. In terms of stomata length and width, the average size of the GMP3 variety mutant was greater than that of the control. The diversity of stomata characters is fairly high due to differences in stomata size between GMP3 and control mutants. With a similarity index of 0.20, the phenetic analysis of 21 mutants of the GMP3 variety revealed that the relationship between mutants and controls was getting further. A six-character principal component analysis revealed that axis I's total variation accounted for 40.54 percent of the variation and had an eigenvalue of 2.43, whereas axis II's contribution to the variation was 19.02 percent and had an eigenvalue of 1.14. The findings indicate that stomata are excellent taxonomic evidence for identifying and analysing sugarcane varieties induced by colchicine-induced breeding.","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139342948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico Determination of Host-Viral Interaction of Apoptotic Mimicry Pathway Proteins During Hepatitis B Viral Pathogenesis","authors":"Prachie Sharma, K. Rawal, K. Kumar","doi":"10.22146/jtbb.72578","DOIUrl":"https://doi.org/10.22146/jtbb.72578","url":null,"abstract":"Viruses are the opportunistic pathogens that have developed several elegant strategies to deploy their host systems for a pathogenic invasion. Viral apoptotic mimicry is characterized by the exposure of host cell phospholipid, the phosphatidylserine which marks the host cell for apoptotic activation. The Hepatitis b virus, an enveloped virus has recently been found to interact with Phosphatidylserine (Ptdser) on the host through its large surface protein experimentally. Nonetheless, the employment of apoptotic mimicry during the pathogenesis of HBV has not been determined.  Therefore, in the present study, we attempt the in-silico exploration of the interaction of the apoptosis initiating receptors activated by Phosphatidylserine Receptors such as TIM3, AXL, MERTK and GAS6 by Hepatitis B Virus L protein. Molecular Docking of Phosphatidylserine Receptor were studied to observe protein – protein interaction against Surface L Protein of Hepatitis B Virus by using online protein  interaction software. It was found from the in-silico studies that Phosphatidylserine Receptors i.e. TIM3 (PDB: 5F71), AXL (PDB: 5U6B), MERTK (PDB: 2POC) and Gas6 (Growth Arrest Specific protein 6) (PDB: 2C5D) have shown effective binding efficacy against Surface L Protein of Hepatitis B Virus, whereas TIM3 (PDB: F71) and Gas6 (PDB: 2C5D) has shown maximum binding energy with respect to both the software used to analyse the protein-protein docking. This interaction study can form the basis of the experimental attempt in understanding the viral-host protein interaction pattern during hepatitis b viral infection.","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43236246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining Moderate and High Resolution of Satellite Images for Characterizing Suitable Habitat for Vegetation and Wildlife","authors":"S. M. Razali, Zaiton Samdin, M. Lion","doi":"10.22146/jtbb.77710","DOIUrl":"https://doi.org/10.22146/jtbb.77710","url":null,"abstract":"Combining different resolution of remote sensing satellites becomes a unique approach for vegetation and wildlife habitat assessment study. Remote sensing technology can reach land and water on the Earth's surface, and it can interpret signals from spectral responses. When these techniques are combined with Geographical Information Systems (GIS), land can be monitored in a variety of ways. Meanwhile, changes in land use led to changes in vegetation on the ground, with natural vegetation being removed from natural forests, leaving a degraded forest. This issue was not investigated for assessing habitat suitability for important plantations such as Eucalyptus plantation. Therefore, the study employed remote sensing and Geographical Information System (GIS) to model suitability of habitat to live and to survive in the Eucalyptus plantation. Normalized Difference Vegetation Index (NDVI) and Normalized Difference Water Index (NDWI) derived from a mathematical equation can demonstrate intensity of greenness of green vegetation in particular area and time, and availability of soil moisture, respectively, is very suitable to model the greenness of the area. WorldView-2 satellite image was pre-proceed, proceed, and classified to produce land use indicator in Sabah Softwoods Berhad plantation majoring Eucalyptus spp. tree planted in Tawau, Sabah. Sentinel and Landsat 8 image were used for vegetation and water stress indicator were downloaded from Land Viewer application. Net Primary Productivity (NPP) at monthly scale was also calculated and ranked the productivity for the suitability mapping. Climatic condition based on monthly precipitation and seasonality derived from ASEAN Specialized Meteorological Centre (ASMC) was employed for ranking its suitability value. In this study, natural forest and oil palm plantation is tested to developed suitability map for vegetation and wildlife habitat to live with. All indicators were ranked 10 to 40 presenting benefit and usefulness of the indicator to vegetation and wildlife in the study area. Then, final classification was made from accumulation of those indicators into 0 to 200 (Not suitable to Highly suitable). The results showed 59.9% of the area classified as moderately suitable, 36.9% highly suitable, 3.2% least suitable and no area was classified as not suitable. This type of study assisted forest managers and policymakers for better managing of their forests for better life of trees and wildlife under their management. The methodology adapted in the study is ecologically sounded and economically viable to be modified and complied in Sustainable Forest Management (SFM) in Malaysia and other tropical forest regions. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46205328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of AtRKD4 Gene and Induction of Somatic Embryo in Transformant of Phalaenopsis amabilis Carrying 35S::GR::AtRKD4","authors":"D. Sundari, N.R.G. Perdana, Windi Mose, Jose Gutierrez -Marcos, E. Semiarti","doi":"10.22146/jtbb.71211","DOIUrl":"https://doi.org/10.22146/jtbb.71211","url":null,"abstract":"Phalaenopsis amabilis (L.) Blume is a native Indonesian orchid that plays an important role in the breeding of orchid’s hybrid worldwide. The high consumer demand causes a decline in the population of orchids for commercial trade. Plant propagation through induction of somatic embryogenesis will be very beneficial, because plants can be obtained in large numbers and uniform. AtRKD4 gene is an important gene in the model plant Arabidopsis thaliana which functions very early in development stage to initiate embryo formation. The AtRKD4 gene has been inserted into the P. amabilis orchid and several transformants have been obtained. This study aims to determine the stability integration of the AtRKD4 gene in the transformant P. amabilis genome and induce somatic embryo formation on transformant orchids. Plantation of leaf explants from P. amabilis transformants on hormone-free New Phalaenopsis basic medium induced somatic embryo formation by 20%.  Anatomical analysis showed that there is no difference stage between anatomy of somatic embryo development pattern in P. amabilis transformant and somatic embryo development pattern of monocot plants in general, PCR analysis using AtRKD4 specific primers showed that the embryos still contained 198 bp fragments of the AtRKD4 gene. In conclusion, the AtRKD4 gene is stably integrated in the P. amabilis orchid genome and can continuously induce the formation of somatic embryo from somatic cells of orchid transformants.","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47590019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Updated Species Check-list of the Indonesian Stingless Bees (Hymenoptera, Apidae, Apinae, Meliponini)","authors":"M. Trianto, Tuty Arisuryanti, Hari Purwanto, Rosichon Ubaidillah","doi":"10.22146/jtbb.77160","DOIUrl":"https://doi.org/10.22146/jtbb.77160","url":null,"abstract":"A catalog provides an index to previous studies in taxonomy, behavioral research, and pollination ecology, thus consolidating the existing knowledge in an accessible format. In this study, we explore the annotated catalog and bibliography of the Indonesian meliponini stingless bees (Hymenoptera, Apidae, Apinae, Meliponini). The catalog format is arranged based on Rasmussen (2008). All available literature was reviewed for compiling this catalog and bibliography. References to a Meliponini genus only were not included in the list of references. Cited references must have used a trackable specific epithet to have been included. In total, Indonesia has 52 recorded stingless bee species across the Indonesian archipelagoes of Sumatera (27 species), Java (13 species), Nusa Tenggara (1 species), Kalimantan (34 species), Sulawesi (8 species), Bali (1 species), Maluku (4 species), and Papua (12 species). After the data was updated, there was an increase in the number of stingless bee species in Indonesia, namely 46 species (before update) to 52 species (after update). An up-to-date, comprehensive taxonomic and biological catalog is fundamental to any comparative evolutionary, ecological, and behavioral research on any group of organisms. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44532741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Identification of Two Mudskipper Species (Oxudercidae: Periophthalmus) from Kulon Progo, Special Region of Yogyakarta, Indonesia","authors":"D. Febriyanti, Katon Waskito Aji, D. Priyono, Tuty Arisuryanti","doi":"10.22146/jtbb.78161","DOIUrl":"https://doi.org/10.22146/jtbb.78161","url":null,"abstract":"Mudskippers are commonly cryptic species, making identification based solely on morphological characteristics challenging. This study used the DNA barcoding method to identify mudskipper species based on the COI mitochondrial gene. The analysis revealed two distinct species, P. kalolo (20 samples) and P. argentilineatus (3 samples) with high GC contents ranging from 42.94-45.2%. The genetic divergence analysis of P. kalolo showed that they divided into two clades, while P. argentilineatus is divided into three clades with two of the clades (C and D)  still conspecific groups, and those two clades with clade E exhibit a genetic distance greater than 3.5%, suggesting the presence of cryptic species. These findings provide valuable insights into the intraspecies genetic diversity of mudskippers in Indonesia, which could have essential implications for conservation efforts and highlight the potential of DNA barcoding as a powerful tool for the identification of cryptic species. Further research combining molecular and morphological identification could lead to a more comprehensive understanding of species identification and help address the challenges posed by cryptic species.","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42833917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autecology of Nepenthes spp. in Peat Swamp and Heath Forest Pematang Gadung, West Kalimantan","authors":"Nofi Utari, Sulistijorini Sulistijorini, N. S. Ariyanti","doi":"10.22146/jtbb.81351","DOIUrl":"https://doi.org/10.22146/jtbb.81351","url":null,"abstract":"Nepenthes occur in various habitat types in West Kalimantan. But some species are categorized as vulnerable on the IUCN red list. Autecological studies of Nepenthes spp. are required for species management and conservation. The study aimed to analyze species diversity, distribution pattern, association, and environmental factors that correlated with Nepenthes spp. at peat swamp and heath forest in Pematang Gadung. Data was collected in September 2021 by purposive sampling on 60 plots measuring 3 x 3 m2 in each habitat type. Species diversity was analyzed based on the presence and abundance of species in the plots. The distribution pattern was determined using a standardized Morisita index (Ip). Meanwhile, the association type was determined based on the Jaccard index (JI). Environmental data were analyzed by Canonical Correspondence Analysis (CCA) using Past Version 4.03. This study found six wild species in a peat swamp and four species in a heath forest. The population of Nepenthes spp. has clumped distribution pattern with Ip>0. Nepenthes spp. are associated with plants such as Barringtonia racemosa, Syzygium sp., Nephrolepis biserrata, and Campnosperma auriculatum. The result of CCA revealed that environmental factors in both habitat types affected the presence of each Nepenthes species. The air temperature, humidity, soil moisture, and light intensity show different influences on different species.  ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47658022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional Contents and Bioactive Compounds among Several Variants of Dolichos lablab: Fundamental Facts for Functional Food Development","authors":"E. Purwanti, F. Hermanto, Wahyu Prihanta, T. Permana, I. G. Wiwekananda","doi":"10.22146/jtbb.81339","DOIUrl":"https://doi.org/10.22146/jtbb.81339","url":null,"abstract":"To date, the data describing various nutritional and secondary metabolites content of Lablab beans is incomplete. Therefore, this study evaluated the nutritional value, secondary metabolites, and antioxidant activity of three different variants of Lablab beans, i.e., brown, black, and cream beans. The results showed that the brown Lablab beans had outperformed other variants according to their nutritional value and flavonoid content with outstanding DPPH scavenging activity. However, the black beans also showed good bioactive contents through their total phenolic percentage with decent reducing activity via the FRAP assay. Those who are keen in developing functional food from Lablab beans should consider this data as a reference. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46614043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Naturalização de Hibiscus sabdariffa L. em região de transição dos Biomas Pantanal, Floresta Amazônica e Cerrado","authors":"Sebastião Marcos Silva Valentim, Petterson Baptista da Luz, Elaidy Laura Oliveira Cardoso, Gabriel Moretto, Antônio Carlos Silva Moreira, Anna Júlia Cardoso Amaral, Bruna Samy de Oliveira Miranda","doi":"10.20873/jbb.uft.cemaf.v11n2.valentim","DOIUrl":"https://doi.org/10.20873/jbb.uft.cemaf.v11n2.valentim","url":null,"abstract":"A espécie Hibiscus sabdariffa L. é originária do continente Africano. Trata-se de uma espécie arbustiva que pode chegar até 2 m de altura, onde devido aos seus vários usos, se espalhou para diversas regiões no mundo, onde atua de forma exótica, podendo se tornar uma espécie naturalizada. Na cidade de Cáceres- MT existe uma pequena população da espécie em uma área antropizada, no qual, de acordo com os moradores locais, as plantas já estão presentes no local há vários anos, mesmo havendo diversas vezes a ocorrência de perturbações na área. Sendo assim, o objetivo do trabalho foi analisar a possível naturalização de Hibiscus sabdariffa em uma localidade antropizada presente na região de transição dos Biomas Pantanal, Floresta Amazônica e Cerrado na cidade de Cáceres- MT. Para a execução do trabalho realizou-se uma pesquisa qualitativa, de natureza exploratória e descritiva, pesquisa bibliográfica sobre Hibiscus sabdariffa e sobre naturalização de plantas. Os dados sobre as perturbações foram coletados através de observações ao decorrer dos anos e por entrevistas com pequenos produtores que residem na comunidade. A pequena população da espécie Hibiscus sabdariffa encontrada na comunidade rural em Cáceres- MT possui as características de naturalização, podendo assim, ser considerada uma espécie naturalizada no local e participante da biodiversidade local. Além disso, o fato de a espécie estar presente na área há vários anos mesmo com as perturbações recorrentes, reforça a ocorrência de naturalização.","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80390923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Desenvolvimento de biocompósito utilizando resíduos agroindustriais e macrofungo","authors":"Álefe Galbiatti De Oliveira","doi":"10.20873/jbb.uft.cemaf.v11n2.oliveira","DOIUrl":"https://doi.org/10.20873/jbb.uft.cemaf.v11n2.oliveira","url":null,"abstract":"A sociedade está passando por uma nova etapa de desenvolvimento tecnológico e científico que urge inovações para aplicação de uma Bioeconomia sustentável a fim de reduzir impactos ambientais como o acúmulo de materiais poliméricos sintéticos derivados do petróleo que, consequentemente, promove a contaminação do solo, devido a sua difícil degradação. Junto a isso, resíduos orgânicos agroindustriais necessitam de aproveitamento, a fim de aumentar seu valor agregado, o que contribui para uma Bioeconomia Circular. Assim, o objetivo deste trabalho é aproveitar a capacidade do micélio fúngico em incorporar resíduos orgânicos, acelerar a decomposição e produzir um composto biodegradável para desenvolver um biocompósito, utilizando resíduos agroindustriais como substrato. A metodologia utilizada iniciou a partir de uma pesquisa bibliográfica para evidenciar um macrofungo com potencial de crescimento rápido, e finalizou com um trabalho prático de cultivo fúngico para o desenvolvimento micelial. Como resultado, identificou-se a condição de produção mais promissora de biocompósito obtido entre as diferentes formulações de substrato considerando suas características físicas, a fim de obter um biocompósito para produção de futuros produtos biodegradáveis.","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"75 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79255694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}