中华临床感染病杂志最新文献

{"title":"The interaction between 2019-nCoV and interferon I","authors":"J. Guan, Z. Chen, H. Zhu","doi":"10.3760/cma.j.issn.1674-2397.2021.02.004","DOIUrl":"https://doi.org/10.3760/cma.j.issn.1674-2397.2021.02.004","url":null,"abstract":"COVID-19 has swept across the world, causing widespread epidemics and millions of life lost worldwide. After infected with 2019-nCoV, the body quickly mobilizes the innate immune response and produces type Ⅰ interferon (IFN-Ⅰ). IFN-Ⅰ plays an important role in virus clearance in the early stage of disease. This article reviews the innate immune recognition after virus infection and the interaction between 2019-nCoV and IFN-Ⅰ, which would be conductive to understanding the pathogenesis and antiviral treatment of COVID-19. © 2021 Chinese Medical Association","PeriodicalId":52394,"journal":{"name":"Chinese Journal of Clinical Infectious Diseases","volume":"14 1","pages":"97-101"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70017692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress on the mechanism of multiple organ dysfunction in patients with COVID-19","authors":"K. Chen, S. Jiang, A. Hu","doi":"10.3760/cma.j.issn.1674-2397.2021.02.005","DOIUrl":"https://doi.org/10.3760/cma.j.issn.1674-2397.2021.02.005","url":null,"abstract":"The pathogen of COVID-19 is 2019-nCoV, which belongs to the beta coronavirus. Angiotensin-converting enzyme 2 (ACE2) is the receptor of 2019-nCoV as the same of SARS-CoV. Most of the severe patients were the elderly with underlying diseases, which may be related to the decrease in the number of naive T cells. In addition to pulmonary symptoms, COVID-19 can also cause multiple organ dysfunction and even multiple organ failure (liver, nervous system, heart, kidney, etc.). Pathogenic mechanisms such as direct virus invasion, cytokine storm, endothelial cells damage, and down-regulation of ACE2 may play important roles in the severity of the disease. © 2021 Chinese Medical Association","PeriodicalId":52394,"journal":{"name":"Chinese Journal of Clinical Infectious Diseases","volume":"14 1","pages":"102-106"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70017301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors for fatal outcome in patients with severe COVID-19: an analysis of 107 cases in Wuhan","authors":"Kai-ming Dai, A. Bao, P. Ye, M. Xu, Qinran Zhang, Yu Zhou, Wanli Jiang, Wubian Jiang, Huimin Wang, Meng Zhu, Lingling Tang, Chengliang Zhu, Yuchen Xia, Ying'an Jiang, Xiufen Zou, Lanjuan Li","doi":"10.3760/CMA.J.ISSN.1674-2397.2020.04.002","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1674-2397.2020.04.002","url":null,"abstract":"Objective: To analyze the risk factors of fatal outcome in patients with severe COVID-19 Methods: The clinical characteristics of 107 patients with severe COVID-19 admitted in Renmin Hospital of Wuhan University from February 12 to March 12, 2020 were retrospectively analyzed During the hospitalization 49 patients died (fatal group) and 58 patients survived (survival group) The clinical characteristics, baseline laboratory findings were analyzed using R and Python statistical software The risk factors of fatal outcome in patients with severe COVID-19 were analyzed with multivariate logistic regression Results: Univariate analysis showed that the two groups had statistically significant differences in age, clinical classification, dry cough, dyspnea and laboratory test indicators (P<0 05 or <0 01) The random forest model was used to rank the significance of the statistically significant variables in the univariate analysis, and the selected variables were included in the binary logistic regression model After stepwise regression analysis, the patient's clinical type, age, neutrophil count, and the proportion of CD3 cells are independent risk factors for death in severe COVID-19 patients Dry cough is an independent protective factor for the death of severe COVID-19 patients Conclusion: COVID-19 patients with fatal outcome are more likely to have suppressed immune function, secondary infection and inflammatory factor storm These factors may work together in severe patients, leading to intractable hypoxemia and multiple organ dysfunction and resulting in fatal outcome of patients The study indicates that timely intervention and treatment measures against above factors may be effective to save the lives of patients with severe COVID-19 Copyright © 2020 by the Chinese Medical Association","PeriodicalId":52394,"journal":{"name":"Chinese Journal of Clinical Infectious Diseases","volume":"13 1","pages":"257-263"},"PeriodicalIF":0.0,"publicationDate":"2020-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49050471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical features and chest CT findings in moderate and severe COVID-19 patients: an analysis of 506 cases from Wuhan Huoshenshan Hospital","authors":"Xu Fang, Jun Cai, Quan Liang, Xiao Li, Yun Bian","doi":"10.3760/CMA.J.ISSN.1674-2397.2020.03.001","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1674-2397.2020.03.001","url":null,"abstract":"Objective: To investigate the clinical features and chest CT findings in moderate and severe COVID-19 patients Methods: A total of 506 patients with COVID-19 treated in Wuhan Huoshenshan Hospital during February 9 to March 9, 2020 were enrolled in the study, including 365 moderate cases and 141 severe cases The clinical features and chest CT findings were retrospectively analyzed Chi-square test and Fisher's exact probability were used for data analysis Results: The proportions of patients with diabetes and hypertension in severe group were significantly higher than those in moderate group (χ2=9 377 and 15 085, P 10 cm and involved all five lung lobes (P<0 01) The severe patients had more imaging signs of air bronchogram, bronchovascular thickening, pleural thickening, mediastinal or hilar lymphnodes enlargement, pleural effusion and pericardial effusion than moderate patients (χ2=33 357, 11 114, 14 580, 5 978, 45 731 and 6 623, P<0 05 or <0 01) Conclusion: There are differences in clinical features and chest CT findings between moderate and severe patients, and chest CT findings can be used as important criteria for clinical classification Copyright © 2020 by the Chinese Medical Association","PeriodicalId":52394,"journal":{"name":"Chinese Journal of Clinical Infectious Diseases","volume":"13 1","pages":"161-166"},"PeriodicalIF":0.0,"publicationDate":"2020-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47593441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early antiviral therapy of abidor combined with lopinavir/ritonavir and re-combinant interferonα-2b in patients with novel coronavirus pneumonia in Zhejiang: A multicenter and prospective study/ 中华临床感染病杂志","authors":"Runan Wei, Nanhong Zheng, Xiangao Jiang, Chun-hong Ma, Xiaowei Xu, Shourong Liu, Yongping Chen, Kaijin Xu, Hainv Gao, Jiansheng Zhu, Q. Shu, J. Sheng, Xiaoqiang Zhang, Minghui Li, Yan Zhang, M. Ma, Xuan Zhang, Shibo Li, Qiujing Wang, L. Ying, Yongjun Zhang, Yun-zhen Shi, Lingyan Fan, Wanjun Yu, Huaying Wang, Dandan Sun, Xiaodong Wang, Jichan Shi, Ying-hu Chen, Xinsheng Xie, Yunqing Chen, Weihong Wang, Zhaowei Tong, Lingling Tang, Meng Zhu, Lingjian Zhang, Lanjuan Li","doi":"10.3760/CMA.J.CN115673-20200224-00069","DOIUrl":"https://doi.org/10.3760/CMA.J.CN115673-20200224-00069","url":null,"abstract":"Objective@#Comparing the benefit of Abidor, lopinavir/ritonavir and recombinant interferon α-2b triple combination antiviral therapy and lopinavir/ritonavir and interferon dual combination antiviral therapy to hospitalized novel coronavirus pneumonia 2019 in Zhejiang province.@*Methods@#A multi-center prospective study was carried out to compare the effect of triple combination antiviral therapy with dual combination antiviral therapy in 15 medical institutions of Zhejiang Province. All patients were treated with recombinant interferon α-2b (5 million U, 2 times/d) aerosol inhalation. 196 patients were treated with abidol (200 mg, 3 times/d) + lopinavir / ritonavir (2 tablets, 1 time/12 h) as the triple combination antiviral treatment group. 41 patients were treated with lopinavir / ritonavir (2 tablets, 1 time/12 h) as the dual combination antiviral treatment group. The patients who received triple combination antiviral therapy were divided into three groups: within 48 hours, 3-5 days and > 5 days after the symptom onset. To explore the therapeutic effects of triple combination antiviral drugs and dual combination antiviral drugs, as well as triple combination antiviral drugs with different antiviral initiate time. SPSS17.0 software was used to analyze the data.@*Results@#The time of virus nucleic acid turning negative was (12.2 ± 4.7) days in the triple combination antiviral drug group, which was shorter than that in the dual combination antiviral drug group [(15.0 ± 5.0) days] (t = 6.159, P 5 days after the symptom onset of triple combination antiviral drug group, the time from the symptom onset to the negative of viral shedding was 13 (10,16.8), 17 (13,22) and 21 (18-24) days respectively (Z = 32.983, P < 0.01), and the time from antiviral therapy to the negative of viral shedding was (11.8±3.9) , (13.5±5.1) and (11.2±4.3) d. The differences among the three groups were statistically significant (Z=32.983 and 6.722, P<0.01 or<0.05).@*Conclusions@#The triple combination antiviral therapy of Abidor, Lopinavir/Litonavir and recombinant interferon α-2b showed shorter viral shedding time and hospitalization time compared with the dual combination antiviral therapy. The earlier the time to initiate triple antiviral treatment, the shorter the time of virus shedding.","PeriodicalId":52394,"journal":{"name":"Chinese Journal of Clinical Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45723365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrospective study of low-to-moderate dose glucocorticoids on viral clearance in patients with novel coronavirus pneumonia","authors":"N. Qin, Cheng Ding, Yongtao Li, Hong Zhao, Jun Liu, Xuan Zhang, Yanfei Chen, Yong-zheng Guo, Liang Yu, H. Ju, Jingjing Tao, Ping Yi, Guan-jing Lang, Jun-wei Su, D. Shi, Wen-rui Wu, Xiaoxin Wu, Ling Yu, J. Sheng, Kaijin Xu","doi":"10.3760/CMA.J.CN115673-20200225-00072","DOIUrl":"https://doi.org/10.3760/CMA.J.CN115673-20200225-00072","url":null,"abstract":"Objective@#To study the effect of low-to-moderate dose glucocorticoid therapy on viral clearance time in patients with COVID-19.@*Methods@#A total of 72 patients diagnosed with COVID-19 from January 19 to February 17, 2020 at the First Affiliated Hospital, School of Medicine, Zhejiang University were recruited. All patients received oral abidol and/or combined lopinavir/ritonavir, darunavir antiviral, and symptomatic supportive care. Among them, 51 patients received methylprednisolone (0.75-1.50 mg·kg-1·d-1) (glucocorticoid treatment group), and 21 patients who did not use glucocorticoid were the control group. The time of stable virologic conversion insputumand the time of radiologic recovery in lungsince onset were compared between the two groups and among the normal patients.The Kruskal-Wallis test or Fisher exact test was used to compare the difference between groups.@*Results@#The median ages of the glucocorticoid group and the control group were 52 [interquartile range (IQR):45, 62] years and 46 (IQR: 32, 56)years, and the differences were significant (P 0.05), and the difference was no statistically significant. The median times from onset to radiologic recovery were 13 (IQR: 11,15) days and 13 (IQR:12,17) days in the two groups, and there was no difference (P>0.05). In ordinary patients, the median timesfrom the onset tostable virologic conversion insputum were no difference (P>0.05), with 13 (IQR:11,18) days in the glucocorticoid group and 13 (IQR:12,15) days in the control group; The median times from onset to radiologic recovery in lungwere also no difference (P>0.05), with 12 (IQR: 10,15)days in the glucocorticoid group and 13 (IQR: 12,17) days inthe control group.@*Conclusions@#Low-to-moderate glucocorticoid treatment has no effect on the time of virus clearance in patients with different clinical types of COVID-19. The glucocorticoid is not recommended since no effectiveness on accelerating the improvement of radiologic recovery in lung has been observed.","PeriodicalId":52394,"journal":{"name":"Chinese Journal of Clinical Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44409180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of clinical features of 153 patients with novel coronavirus pneumonia in Chongqing/ 中华临床感染病杂志","authors":"Qiuhua Wan, A. Shi, Ting He, Li-xin Tang","doi":"10.3760/CMA.J.CN115673-20200212-00030","DOIUrl":"https://doi.org/10.3760/CMA.J.CN115673-20200212-00030","url":null,"abstract":"Objective \u0000To analyze the clinical data of 153 patients with novel coronavirus pneumonia (COVID-19) in chongqing ,and provide reference and thinking for the diagnosis and treatment. \u0000 \u0000 \u0000Methods \u0000Analyze the clinical data, laboratory examination and chest imaging characteristics of 153 COVID-19 patients in Chongqing Public Health Medical Center from January 26 to February 5, 2020. According to the relevant diagnostic criteria ,patients were divided into non-severe group(n=132) and severe group(n=21),and analyze the correlation between serum index changes and disease severity. \u0000 \u0000 \u0000Results \u0000Combined with diabetes and chronic respiratory diseases, the severity of the disease was statistically significant (χ2=11.04和6.94, P<0.05). No symptoms were found in patients with mild illness (χ2=4.09, P<0.05) .The proportion of fever and muscle soreness in the severe group was higher than that in the non-severe group (χ2=4.40 and 22.67,P<0.05).Among the concomitant symptoms, the proportion of cough and shortness of breath in the severe group was higher than that in the non-severe group (χ2=8.46 and 4.80,P<0.05).C-reactive protein and d-dimer were higher in the severe group than in the non-severe group (t=43.44 and 37.13, P<0.05), and the number of CD3+T lymphocyte cells, CD4+T lymphocyte cells and CD8+T lymphocyte cells in the severe group was lower than that in the non-severe group (Z=27.25, 20.60 and 17.36, P<0.05).Compared with the non-severe group, both lungs and the right lung lower lobe were more susceptible to involved (χ2=6.95和20.39, P<0.05) . \u0000 \u0000 \u0000Conclusion \u0000Severity of COVID-19 was associated with underlying disease, symptoms, site of involvement, C-reactive protein, d-dimer, CD3+T lymphocyte count, CD4+T lymphocyte count, and CD8+T lymphocyte count. \u0000 \u0000 \u0000Key words: \u00002019 novel Coronavirus; Clinical features; Severe disease","PeriodicalId":52394,"journal":{"name":"Chinese Journal of Clinical Infectious Diseases","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41371396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the hemolysin and virulence-related genes of incomplete hemolysis Staphylococcus aureus","authors":"Yi Zheng, Xiao-fang Xie, Hong Du, Haifang Zhang, Xueming Zhu, Min Wang, Hua Yang, Aiqing Li, Y. Jiang, Hui-qin Zhou","doi":"10.3760/CMA.J.ISSN.1674-2397.2016.03.006","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1674-2397.2016.03.006","url":null,"abstract":"Objective \u0000To analyze hemolysin and virulence-related genes in incomplete hemolytic Staphylococcus aureus. \u0000 \u0000 \u0000Methods \u0000Fifty strains of incomplete hemolytic Staphylococcus aureus were isolated from patients admitted in the Second Affiliated Hospital of Soochow University during 2013 and 2014, and the isolates with complete hemolytic phenotype were also collected at the same period as the control strains. All the strains were inoculated and subcultured on four kinds of sheep blood agar plates supplied by different manufacturers to compare their hemolytic phenotype. The relative mRNA expressions of hemolysin genes (hla, hlb, hlc, hld) in standard strain, complete and incomplete hemolytic phenotype strains were detected by real-time quantitative polymerase chain reaction (RT-qPCR), and valued by 2-△△Ct method. t test was used to compare mRNA expressions of hemolysin genes. Western blot was performed to analyze the expression of α-hemolysin. Antibiotic susceptibility test of incomplete hemolytic strains was performed using broth microdilution method. Resistant gene mecA and virulence genes pvl, tst were detected by PCR. \u0000 \u0000 \u0000Results \u0000The steady and hereditary incomplete hemolysis was observed in 50 strains of incomplete hemolytic Staphylococcus aureus on the sheep blood agar plates from different suppliers. Taking mRNA expression of hla, hlb, hlc, hld in standard strain as 1, the relative mRNA expressions of hemolysin genes in incomplete hemolytic strains were 0.02, 7.51, 0.06 and 0.12 respectively, there were statistical differences between standard strain and incomplete hemolytic strains (t=8.46, -56.40, 8.12 and 7.61, all P<0.05). And the expression of α-hemolysin was decreased in incomplete hemolytic strains. All the strains were identified as methicillin resistant Staphylococcus aureus (MRSA). Three strains exhibited different minimum inhibitory concentrations of teicoplanin and linezolid after subcultured, but the differences had no impact on the final results of antibiotic susceptibility test. mecA, pvl and tst genes were positive in incomplete hemolytic strains. \u0000 \u0000 \u0000Conclusion \u0000Staphylococcus aureus with incomplete hemolytic phenotype is methicillin resistant with higher expression of β-hemolysin and lower expressions of α-hemolysin, γ-hemolysin and δ-hemolysin. It carries plv and tst virulence genes and is of high virulence. \u0000 \u0000 \u0000Key words: \u0000Staphylococcus aureus; Incomplete hemolysis; Hemolysin; Drug resistance; Virulence","PeriodicalId":52394,"journal":{"name":"Chinese Journal of Clinical Infectious Diseases","volume":"9 1","pages":"236-242"},"PeriodicalIF":0.0,"publicationDate":"2016-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70017674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}